This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.81 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 31,358 (1.1%) Reads that were too short: 8,074 (0.3%) Reads written (passing filters): 23,284 (0.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 4,149,299 bp (0.5%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 31358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 85 714.6 0 85 7 82 714.6 0 82 8 117 714.6 0 117 9 1737 714.6 0 1737 10 55 714.6 0 55 11 52 714.6 0 52 12 46 714.6 0 46 13 45 714.6 0 45 14 57 714.6 0 57 15 40 714.6 0 40 16 42 714.6 0 42 17 47 714.6 0 47 18 50 714.6 0 50 19 51 714.6 0 51 20 59 714.6 0 59 21 50 714.6 0 50 22 30 714.6 0 30 23 35 714.6 0 35 24 42 714.6 0 42 25 30 714.6 0 30 26 37 714.6 0 37 27 32 714.6 0 32 28 38 714.6 0 38 29 36 714.6 0 36 30 35 714.6 0 35 31 48 714.6 0 48 32 42 714.6 0 42 33 37 714.6 0 37 34 34 714.6 0 34 35 44 714.6 0 44 36 53 714.6 0 53 37 40 714.6 0 40 38 46 714.6 0 46 39 54 714.6 0 54 40 50 714.6 0 50 41 42 714.6 0 42 42 43 714.6 0 43 43 30 714.6 0 30 44 37 714.6 0 37 45 34 714.6 0 34 46 56 714.6 0 56 47 31 714.6 0 31 48 35 714.6 0 35 49 44 714.6 0 44 50 33 714.6 0 33 51 23 714.6 0 23 52 137 714.6 0 137 53 91 714.6 0 91 54 58 714.6 0 58 55 40 714.6 0 40 56 46 714.6 0 46 57 31 714.6 0 31 58 37 714.6 0 37 59 45 714.6 0 45 60 40 714.6 0 40 61 50 714.6 0 50 62 32 714.6 0 32 63 63 714.6 0 63 64 90 714.6 0 90 65 50 714.6 0 50 66 40 714.6 0 40 67 39 714.6 0 39 68 39 714.6 0 39 69 43 714.6 0 43 70 50 714.6 0 50 71 52 714.6 0 52 72 77 714.6 0 77 73 752 714.6 0 752 74 801 714.6 0 801 75 212 714.6 0 212 76 33 714.6 0 33 77 31 714.6 0 31 78 28 714.6 0 28 79 47 714.6 0 47 80 53 714.6 0 53 81 34 714.6 0 34 82 39 714.6 0 39 83 57 714.6 0 57 84 75 714.6 0 75 85 94 714.6 0 94 86 191 714.6 0 191 87 140 714.6 0 140 88 177 714.6 0 177 89 124 714.6 0 124 90 57 714.6 0 57 91 57 714.6 0 57 92 75 714.6 0 75 93 44 714.6 0 44 94 55 714.6 0 55 95 83 714.6 0 83 96 34 714.6 0 34 97 37 714.6 0 37 98 33 714.6 0 33 99 36 714.6 0 36 100 44 714.6 0 44 101 43 714.6 0 43 102 36 714.6 0 36 103 21 714.6 0 21 104 21 714.6 0 21 105 32 714.6 0 32 106 21 714.6 0 21 107 35 714.6 0 35 108 54 714.6 0 54 109 54 714.6 0 54 110 27 714.6 0 27 111 30 714.6 0 30 112 36 714.6 0 36 113 33 714.6 0 33 114 38 714.6 0 38 115 72 714.6 0 72 116 51 714.6 0 51 117 34 714.6 0 34 118 41 714.6 0 41 119 34 714.6 0 34 120 34 714.6 0 34 121 48 714.6 0 48 122 63 714.6 0 63 123 40 714.6 0 40 124 31 714.6 0 31 125 55 714.6 0 55 126 44 714.6 0 44 127 35 714.6 0 35 128 65 714.6 0 65 129 81 714.6 0 81 130 103 714.6 0 103 131 69 714.6 0 69 132 41 714.6 0 41 133 75 714.6 0 75 134 48 714.6 0 48 135 108 714.6 0 108 136 112 714.6 0 112 137 172 714.6 0 172 138 166 714.6 0 166 139 62 714.6 0 62 140 47 714.6 0 47 141 87 714.6 0 87 142 93 714.6 0 93 143 56 714.6 0 56 144 51 714.6 0 51 145 85 714.6 0 85 146 202 714.6 0 202 147 819 714.6 0 819 148 772 714.6 0 772 149 71 714.6 0 71 150 75 714.6 0 75 151 46 714.6 0 46 152 64 714.6 0 64 153 99 714.6 0 99 154 87 714.6 0 87 155 144 714.6 0 144 156 172 714.6 0 172 157 171 714.6 0 171 158 540 714.6 0 540 159 311 714.6 0 311 160 248 714.6 0 248 161 110 714.6 0 110 162 136 714.6 0 136 163 72 714.6 0 72 164 44 714.6 0 44 165 83 714.6 0 83 166 216 714.6 0 216 167 172 714.6 0 172 168 109 714.6 0 109 169 153 714.6 0 153 170 251 714.6 0 251 171 121 714.6 0 121 172 84 714.6 0 84 173 83 714.6 0 83 174 628 714.6 0 628 175 628 714.6 0 628 176 243 714.6 0 243 177 106 714.6 0 106 178 83 714.6 0 83 179 87 714.6 0 87 180 925 714.6 0 925 181 837 714.6 0 837 182 161 714.6 0 161 183 157 714.6 0 157 184 110 714.6 0 110 185 117 714.6 0 117 186 176 714.6 0 176 187 935 714.6 0 935 188 571 714.6 0 571 189 131 714.6 0 131 190 313 714.6 0 313 191 76 714.6 0 76 192 106 714.6 0 106 193 71 714.6 0 71 194 75 714.6 0 75 195 54 714.6 0 54 196 37 714.6 0 37 197 34 714.6 0 34 198 41 714.6 0 41 199 54 714.6 0 54 200 66 714.6 0 66 201 44 714.6 0 44 202 71 714.6 0 71 203 69 714.6 0 69 204 122 714.6 0 122 205 161 714.6 0 161 206 93 714.6 0 93 207 73 714.6 0 73 208 68 714.6 0 68 209 75 714.6 0 75 210 52 714.6 0 52 211 50 714.6 0 50 212 57 714.6 0 57 213 63 714.6 0 63 214 79 714.6 0 79 215 91 714.6 0 91 216 75 714.6 0 75 217 69 714.6 0 69 218 88 714.6 0 88 219 62 714.6 0 62 220 46 714.6 0 46 221 60 714.6 0 60 222 75 714.6 0 75 223 59 714.6 0 59 224 55 714.6 0 55 225 80 714.6 0 80 226 63 714.6 0 63 227 155 714.6 0 155 228 276 714.6 0 276 229 123 714.6 0 123 230 62 714.6 0 62 231 79 714.6 0 79 232 75 714.6 0 75 233 55 714.6 0 55 234 71 714.6 0 71 235 63 714.6 0 63 236 55 714.6 0 55 237 68 714.6 0 68 238 76 714.6 0 76 239 32 714.6 0 32 240 53 714.6 0 53 241 40 714.6 0 40 242 46 714.6 0 46 243 57 714.6 0 57 244 63 714.6 0 63 245 62 714.6 0 62 246 63 714.6 0 63 247 60 714.6 0 60 248 77 714.6 0 77 249 65 714.6 0 65 250 71 714.6 0 71 251 190 714.6 0 190 252 940 714.6 0 940 253 238 714.6 0 238 254 222 714.6 0 222 255 139 714.6 0 139 256 59 714.6 0 59 257 70 714.6 0 70 258 75 714.6 0 75 259 30 714.6 0 30 260 47 714.6 0 47 261 80 714.6 0 80 262 199 714.6 0 199 263 123 714.6 0 123 264 88 714.6 0 88 265 60 714.6 0 60 266 44 714.6 0 44 267 44 714.6 0 44 268 48 714.6 0 48 269 46 714.6 0 46 270 37 714.6 0 37 271 30 714.6 0 30 272 50 714.6 0 50 273 74 714.6 0 74 274 47 714.6 0 47 275 40 714.6 0 40 276 32 714.6 0 32 277 50 714.6 0 50 278 87 714.6 0 87 279 72 714.6 0 72 280 37 714.6 0 37 281 66 714.6 0 66 282 68 714.6 0 68 283 89 714.6 0 89 284 26 714.6 0 26 285 71 714.6 0 71 286 69 714.6 0 69 287 35 714.6 0 35 288 30 714.6 0 30 289 69 714.6 0 69 290 36 714.6 0 36 291 79 714.6 0 79 292 52 714.6 0 52 293 31 714.6 0 31 294 42 714.6 0 42 295 56 714.6 0 56 296 26 714.6 0 26 297 53 714.6 0 53 298 39 714.6 0 39 299 52 714.6 0 52 300 48 714.6 0 48 301 36 714.6 0 36 Finished in 0.91 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 23,284 Reads with adapters: 1,819 (7.8%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 1,809 (7.8%) Total basepairs processed: 4,149,299 bp Total written (filtered): 492,035 bp (11.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1819 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 21 0.0 3 0 20 1 20 1741 0.0 4 1623 103 11 2 2 21 7 0.0 4 3 2 0 1 1 22 1 0.0 4 0 0 0 1 23 34 0.0 4 29 2 1 1 1 24 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 2 112 1 0.0 4 0 0 0 0 1 155 5 0.0 4 0 0 0 0 5 177 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 1,809 Reads with adapters: 122 (6.7%) Reads written (passing filters): 1,809 (100.0%) Total basepairs processed: 492,035 bp Total written (filtered): 490,300 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 122 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 4.9% G: 87.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 26 0.0 0 2 24 10 63 0.0 1 1 62 11 31 0.0 1 0 31 260 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.