This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.49 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 66,923 (2.3%) Reads that were too short: 24,398 (0.8%) Reads written (passing filters): 42,525 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,986,326 bp (1.0%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 66923 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 175 714.6 0 175 7 2000 714.6 0 2000 8 6822 714.6 0 6822 9 79 714.6 0 79 10 35 714.6 0 35 11 41 714.6 0 41 12 43 714.6 0 43 13 37 714.6 0 37 14 48 714.6 0 48 15 36 714.6 0 36 16 34 714.6 0 34 17 50 714.6 0 50 18 55 714.6 0 55 19 49 714.6 0 49 20 38 714.6 0 38 21 64 714.6 0 64 22 68 714.6 0 68 23 414 714.6 0 414 24 48 714.6 0 48 25 39 714.6 0 39 26 37 714.6 0 37 27 41 714.6 0 41 28 42 714.6 0 42 29 52 714.6 0 52 30 68 714.6 0 68 31 275 714.6 0 275 32 51 714.6 0 51 33 79 714.6 0 79 34 51 714.6 0 51 35 73 714.6 0 73 36 36 714.6 0 36 37 41 714.6 0 41 38 50 714.6 0 50 39 77 714.6 0 77 40 59 714.6 0 59 41 77 714.6 0 77 42 40 714.6 0 40 43 80 714.6 0 80 44 51 714.6 0 51 45 47 714.6 0 47 46 50 714.6 0 50 47 43 714.6 0 43 48 28 714.6 0 28 49 43 714.6 0 43 50 42 714.6 0 42 51 52 714.6 0 52 52 56 714.6 0 56 53 49 714.6 0 49 54 36 714.6 0 36 55 58 714.6 0 58 56 47 714.6 0 47 57 104 714.6 0 104 58 48 714.6 0 48 59 54 714.6 0 54 60 57 714.6 0 57 61 54 714.6 0 54 62 40 714.6 0 40 63 62 714.6 0 62 64 92 714.6 0 92 65 9178 714.6 0 9178 66 341 714.6 0 341 67 43 714.6 0 43 68 38 714.6 0 38 69 46 714.6 0 46 70 57 714.6 0 57 71 54 714.6 0 54 72 40 714.6 0 40 73 46 714.6 0 46 74 43 714.6 0 43 75 50 714.6 0 50 76 47 714.6 0 47 77 37 714.6 0 37 78 43 714.6 0 43 79 48 714.6 0 48 80 37 714.6 0 37 81 67 714.6 0 67 82 69 714.6 0 69 83 87 714.6 0 87 84 82 714.6 0 82 85 125 714.6 0 125 86 171 714.6 0 171 87 70 714.6 0 70 88 45 714.6 0 45 89 36 714.6 0 36 90 52 714.6 0 52 91 61 714.6 0 61 92 79 714.6 0 79 93 75 714.6 0 75 94 84 714.6 0 84 95 115 714.6 0 115 96 138 714.6 0 138 97 99 714.6 0 99 98 134 714.6 0 134 99 168 714.6 0 168 100 167 714.6 0 167 101 208 714.6 0 208 102 250 714.6 0 250 103 285 714.6 0 285 104 169 714.6 0 169 105 85 714.6 0 85 106 48 714.6 0 48 107 64 714.6 0 64 108 50 714.6 0 50 109 40 714.6 0 40 110 100 714.6 0 100 111 142 714.6 0 142 112 120 714.6 0 120 113 148 714.6 0 148 114 97 714.6 0 97 115 94 714.6 0 94 116 138 714.6 0 138 117 140 714.6 0 140 118 148 714.6 0 148 119 117 714.6 0 117 120 80 714.6 0 80 121 66 714.6 0 66 122 91 714.6 0 91 123 209 714.6 0 209 124 246 714.6 0 246 125 239 714.6 0 239 126 100 714.6 0 100 127 119 714.6 0 119 128 96 714.6 0 96 129 114 714.6 0 114 130 121 714.6 0 121 131 151 714.6 0 151 132 217 714.6 0 217 133 270 714.6 0 270 134 165 714.6 0 165 135 195 714.6 0 195 136 124 714.6 0 124 137 183 714.6 0 183 138 289 714.6 0 289 139 301 714.6 0 301 140 153 714.6 0 153 141 142 714.6 0 142 142 264 714.6 0 264 143 400 714.6 0 400 144 483 714.6 0 483 145 444 714.6 0 444 146 137 714.6 0 137 147 126 714.6 0 126 148 120 714.6 0 120 149 133 714.6 0 133 150 133 714.6 0 133 151 166 714.6 0 166 152 117 714.6 0 117 153 128 714.6 0 128 154 102 714.6 0 102 155 85 714.6 0 85 156 106 714.6 0 106 157 132 714.6 0 132 158 234 714.6 0 234 159 201 714.6 0 201 160 190 714.6 0 190 161 108 714.6 0 108 162 171 714.6 0 171 163 119 714.6 0 119 164 135 714.6 0 135 165 115 714.6 0 115 166 120 714.6 0 120 167 204 714.6 0 204 168 222 714.6 0 222 169 234 714.6 0 234 170 309 714.6 0 309 171 365 714.6 0 365 172 311 714.6 0 311 173 158 714.6 0 158 174 88 714.6 0 88 175 89 714.6 0 89 176 123 714.6 0 123 177 264 714.6 0 264 178 269 714.6 0 269 179 212 714.6 0 212 180 233 714.6 0 233 181 125 714.6 0 125 182 123 714.6 0 123 183 182 714.6 0 182 184 292 714.6 0 292 185 239 714.6 0 239 186 172 714.6 0 172 187 381 714.6 0 381 188 283 714.6 0 283 189 267 714.6 0 267 190 144 714.6 0 144 191 123 714.6 0 123 192 145 714.6 0 145 193 150 714.6 0 150 194 251 714.6 0 251 195 172 714.6 0 172 196 184 714.6 0 184 197 190 714.6 0 190 198 350 714.6 0 350 199 309 714.6 0 309 200 121 714.6 0 121 201 69 714.6 0 69 202 84 714.6 0 84 203 88 714.6 0 88 204 169 714.6 0 169 205 165 714.6 0 165 206 203 714.6 0 203 207 188 714.6 0 188 208 132 714.6 0 132 209 104 714.6 0 104 210 221 714.6 0 221 211 380 714.6 0 380 212 280 714.6 0 280 213 196 714.6 0 196 214 637 714.6 0 637 215 732 714.6 0 732 216 747 714.6 0 747 217 176 714.6 0 176 218 165 714.6 0 165 219 202 714.6 0 202 220 140 714.6 0 140 221 104 714.6 0 104 222 101 714.6 0 101 223 664 714.6 0 664 224 685 714.6 0 685 225 438 714.6 0 438 226 167 714.6 0 167 227 165 714.6 0 165 228 212 714.6 0 212 229 182 714.6 0 182 230 128 714.6 0 128 231 103 714.6 0 103 232 74 714.6 0 74 233 94 714.6 0 94 234 102 714.6 0 102 235 135 714.6 0 135 236 147 714.6 0 147 237 180 714.6 0 180 238 254 714.6 0 254 239 224 714.6 0 224 240 335 714.6 0 335 241 125 714.6 0 125 242 76 714.6 0 76 243 87 714.6 0 87 244 88 714.6 0 88 245 169 714.6 0 169 246 314 714.6 0 314 247 512 714.6 0 512 248 231 714.6 0 231 249 72 714.6 0 72 250 49 714.6 0 49 251 47 714.6 0 47 252 56 714.6 0 56 253 107 714.6 0 107 254 150 714.6 0 150 255 160 714.6 0 160 256 210 714.6 0 210 257 282 714.6 0 282 258 300 714.6 0 300 259 181 714.6 0 181 260 147 714.6 0 147 261 82 714.6 0 82 262 107 714.6 0 107 263 269 714.6 0 269 264 340 714.6 0 340 265 570 714.6 0 570 266 174 714.6 0 174 267 268 714.6 0 268 268 195 714.6 0 195 269 137 714.6 0 137 270 62 714.6 0 62 271 153 714.6 0 153 272 1068 714.6 0 1068 273 1374 714.6 0 1374 274 1106 714.6 0 1106 275 168 714.6 0 168 276 155 714.6 0 155 277 182 714.6 0 182 278 217 714.6 0 217 279 208 714.6 0 208 280 143 714.6 0 143 281 90 714.6 0 90 282 56 714.6 0 56 283 151 714.6 0 151 284 192 714.6 0 192 285 303 714.6 0 303 286 380 714.6 0 380 287 468 714.6 0 468 288 189 714.6 0 189 289 67 714.6 0 67 290 45 714.6 0 45 291 59 714.6 0 59 292 129 714.6 0 129 293 271 714.6 0 271 294 220 714.6 0 220 295 127 714.6 0 127 296 81 714.6 0 81 297 58 714.6 0 58 298 74 714.6 0 74 299 190 714.6 0 190 300 408 714.6 0 408 301 996 714.6 0 996 Finished in 1.60 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 42,525 Reads with adapters: 1,842 (4.3%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 1,829 (4.3%) Total basepairs processed: 8,986,326 bp Total written (filtered): 502,719 bp (5.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1842 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 51 0.0 3 0 8 43 17 1738 0.0 3 1694 29 14 1 18 8 0.0 3 3 3 2 19 2 0.0 3 0 0 0 2 20 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 1 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 1 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 4 0.0 3 0 0 0 4 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 135 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 224 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 1,829 Reads with adapters: 55 (3.0%) Reads written (passing filters): 1,829 (100.0%) Total basepairs processed: 502,719 bp Total written (filtered): 502,551 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 55 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 61.8% G: 14.5% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 54 28.6 0 54 6 1 0.4 0 1