This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.64 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 205,699 (7.0%) Reads that were too short: 99,004 (3.4%) Reads written (passing filters): 106,695 (3.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 17,588,205 bp (2.0%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 205699 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 41 714.6 0 41 7 185 714.6 0 185 8 3340 714.6 0 3340 9 17 714.6 0 17 10 44 714.6 0 44 11 35 714.6 0 35 12 33 714.6 0 33 13 230 714.6 0 230 14 94 714.6 0 94 15 77 714.6 0 77 16 22 714.6 0 22 17 23 714.6 0 23 18 21 714.6 0 21 19 13 714.6 0 13 20 10 714.6 0 10 21 14 714.6 0 14 22 17 714.6 0 17 23 18 714.6 0 18 24 20 714.6 0 20 25 29 714.6 0 29 26 42 714.6 0 42 27 70 714.6 0 70 28 18 714.6 0 18 29 10 714.6 0 10 30 11 714.6 0 11 31 10 714.6 0 10 32 17 714.6 0 17 33 19 714.6 0 19 34 22 714.6 0 22 35 15 714.6 0 15 36 13 714.6 0 13 37 15 714.6 0 15 38 590 714.6 0 590 39 20 714.6 0 20 40 8 714.6 0 8 41 19 714.6 0 19 42 20 714.6 0 20 43 11 714.6 0 11 44 13 714.6 0 13 45 9 714.6 0 9 46 9 714.6 0 9 47 22 714.6 0 22 48 19 714.6 0 19 49 17 714.6 0 17 50 25 714.6 0 25 51 14 714.6 0 14 52 12 714.6 0 12 53 17 714.6 0 17 54 38 714.6 0 38 55 18 714.6 0 18 56 11 714.6 0 11 57 45 714.6 0 45 58 15 714.6 0 15 59 18 714.6 0 18 60 23 714.6 0 23 61 35 714.6 0 35 62 45 714.6 0 45 63 12 714.6 0 12 64 17 714.6 0 17 65 17 714.6 0 17 66 25 714.6 0 25 67 13 714.6 0 13 68 12 714.6 0 12 69 18 714.6 0 18 70 18 714.6 0 18 71 18 714.6 0 18 72 35 714.6 0 35 73 60 714.6 0 60 74 96 714.6 0 96 75 254 714.6 0 254 76 463 714.6 0 463 77 198 714.6 0 198 78 316 714.6 0 316 79 294 714.6 0 294 80 593 714.6 0 593 81 604 714.6 0 604 82 1236 714.6 0 1236 83 906 714.6 0 906 84 411 714.6 0 411 85 742 714.6 0 742 86 430 714.6 0 430 87 766 714.6 0 766 88 1340 714.6 0 1340 89 1482 714.6 0 1482 90 916 714.6 0 916 91 731 714.6 0 731 92 228 714.6 0 228 93 525 714.6 0 525 94 976 714.6 0 976 95 632 714.6 0 632 96 585 714.6 0 585 97 563 714.6 0 563 98 595 714.6 0 595 99 521 714.6 0 521 100 488 714.6 0 488 101 359 714.6 0 359 102 703 714.6 0 703 103 1364 714.6 0 1364 104 495 714.6 0 495 105 503 714.6 0 503 106 536 714.6 0 536 107 503 714.6 0 503 108 209 714.6 0 209 109 243 714.6 0 243 110 429 714.6 0 429 111 1019 714.6 0 1019 112 1003 714.6 0 1003 113 736 714.6 0 736 114 761 714.6 0 761 115 500 714.6 0 500 116 414 714.6 0 414 117 358 714.6 0 358 118 815 714.6 0 815 119 943 714.6 0 943 120 1155 714.6 0 1155 121 794 714.6 0 794 122 606 714.6 0 606 123 837 714.6 0 837 124 837 714.6 0 837 125 558 714.6 0 558 126 636 714.6 0 636 127 913 714.6 0 913 128 731 714.6 0 731 129 634 714.6 0 634 130 654 714.6 0 654 131 540 714.6 0 540 132 717 714.6 0 717 133 738 714.6 0 738 134 921 714.6 0 921 135 772 714.6 0 772 136 587 714.6 0 587 137 672 714.6 0 672 138 727 714.6 0 727 139 1260 714.6 0 1260 140 1618 714.6 0 1618 141 1113 714.6 0 1113 142 667 714.6 0 667 143 601 714.6 0 601 144 861 714.6 0 861 145 808 714.6 0 808 146 792 714.6 0 792 147 2553 714.6 0 2553 148 854 714.6 0 854 149 530 714.6 0 530 150 571 714.6 0 571 151 758 714.6 0 758 152 889 714.6 0 889 153 915 714.6 0 915 154 757 714.6 0 757 155 805 714.6 0 805 156 653 714.6 0 653 157 754 714.6 0 754 158 888 714.6 0 888 159 984 714.6 0 984 160 2019 714.6 0 2019 161 1953 714.6 0 1953 162 1307 714.6 0 1307 163 673 714.6 0 673 164 850 714.6 0 850 165 715 714.6 0 715 166 533 714.6 0 533 167 519 714.6 0 519 168 908 714.6 0 908 169 911 714.6 0 911 170 602 714.6 0 602 171 513 714.6 0 513 172 620 714.6 0 620 173 935 714.6 0 935 174 664 714.6 0 664 175 638 714.6 0 638 176 810 714.6 0 810 177 913 714.6 0 913 178 699 714.6 0 699 179 830 714.6 0 830 180 968 714.6 0 968 181 1135 714.6 0 1135 182 1347 714.6 0 1347 183 840 714.6 0 840 184 754 714.6 0 754 185 881 714.6 0 881 186 1027 714.6 0 1027 187 1954 714.6 0 1954 188 1501 714.6 0 1501 189 1240 714.6 0 1240 190 797 714.6 0 797 191 715 714.6 0 715 192 577 714.6 0 577 193 769 714.6 0 769 194 498 714.6 0 498 195 621 714.6 0 621 196 1053 714.6 0 1053 197 1102 714.6 0 1102 198 661 714.6 0 661 199 531 714.6 0 531 200 685 714.6 0 685 201 1120 714.6 0 1120 202 1731 714.6 0 1731 203 953 714.6 0 953 204 807 714.6 0 807 205 716 714.6 0 716 206 1033 714.6 0 1033 207 1131 714.6 0 1131 208 969 714.6 0 969 209 870 714.6 0 870 210 956 714.6 0 956 211 1025 714.6 0 1025 212 917 714.6 0 917 213 791 714.6 0 791 214 1012 714.6 0 1012 215 767 714.6 0 767 216 756 714.6 0 756 217 764 714.6 0 764 218 875 714.6 0 875 219 1017 714.6 0 1017 220 1015 714.6 0 1015 221 1122 714.6 0 1122 222 1263 714.6 0 1263 223 935 714.6 0 935 224 757 714.6 0 757 225 782 714.6 0 782 226 1067 714.6 0 1067 227 850 714.6 0 850 228 765 714.6 0 765 229 819 714.6 0 819 230 1223 714.6 0 1223 231 1360 714.6 0 1360 232 1096 714.6 0 1096 233 977 714.6 0 977 234 1014 714.6 0 1014 235 747 714.6 0 747 236 441 714.6 0 441 237 671 714.6 0 671 238 998 714.6 0 998 239 1168 714.6 0 1168 240 1017 714.6 0 1017 241 1466 714.6 0 1466 242 1180 714.6 0 1180 243 852 714.6 0 852 244 747 714.6 0 747 245 943 714.6 0 943 246 891 714.6 0 891 247 557 714.6 0 557 248 500 714.6 0 500 249 473 714.6 0 473 250 987 714.6 0 987 251 1414 714.6 0 1414 252 1082 714.6 0 1082 253 790 714.6 0 790 254 788 714.6 0 788 255 829 714.6 0 829 256 896 714.6 0 896 257 771 714.6 0 771 258 621 714.6 0 621 259 528 714.6 0 528 260 723 714.6 0 723 261 822 714.6 0 822 262 955 714.6 0 955 263 755 714.6 0 755 264 872 714.6 0 872 265 757 714.6 0 757 266 943 714.6 0 943 267 730 714.6 0 730 268 589 714.6 0 589 269 741 714.6 0 741 270 1303 714.6 0 1303 271 1049 714.6 0 1049 272 865 714.6 0 865 273 982 714.6 0 982 274 962 714.6 0 962 275 1051 714.6 0 1051 276 1415 714.6 0 1415 277 1799 714.6 0 1799 278 1352 714.6 0 1352 279 1109 714.6 0 1109 280 852 714.6 0 852 281 853 714.6 0 853 282 1211 714.6 0 1211 283 1414 714.6 0 1414 284 1302 714.6 0 1302 285 1114 714.6 0 1114 286 601 714.6 0 601 287 640 714.6 0 640 288 869 714.6 0 869 289 1149 714.6 0 1149 290 1119 714.6 0 1119 291 1092 714.6 0 1092 292 998 714.6 0 998 293 906 714.6 0 906 294 1045 714.6 0 1045 295 1349 714.6 0 1349 296 875 714.6 0 875 297 855 714.6 0 855 298 1125 714.6 0 1125 299 1196 714.6 0 1196 300 1870 714.6 0 1870 301 3303 714.6 0 3303 Finished in 3.77 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 106,695 Reads with adapters: 3,529 (3.3%) Reads that were too short: 59 (0.1%) Reads written (passing filters): 3,470 (3.3%) Total basepairs processed: 17,588,205 bp Total written (filtered): 944,326 bp (5.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3529 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 3 0.0 3 0 0 2 1 19 33 0.0 3 0 28 3 2 20 3392 0.0 4 3280 96 13 2 1 21 17 0.0 4 2 14 0 0 1 22 1 0.0 4 0 0 0 0 1 23 6 0.0 4 0 0 0 0 6 26 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 0 2 75 3 0.0 4 0 0 0 0 3 77 2 0.0 4 0 0 0 0 2 80 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 91 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 104 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 1 1 113 1 0.0 4 0 0 0 1 115 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,470 Reads with adapters: 385 (11.1%) Reads written (passing filters): 3,470 (100.0%) Total basepairs processed: 944,326 bp Total written (filtered): 938,950 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 385 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 1.6% G: 91.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 54.2 0 1 9 4 0.0 0 0 4 10 34 0.0 1 1 33 11 340 0.0 1 4 336 209 4 0.0 1 4 210 1 0.0 1 1 211 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.