This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.83 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 253,688 (8.7%) Reads that were too short: 69,017 (2.4%) Reads written (passing filters): 184,671 (6.3%) Total basepairs processed: 881,011,047 bp Total written (filtered): 39,102,650 bp (4.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 253688 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2082 714.6 0 2082 7 63402 714.6 0 63402 8 284 714.6 0 284 9 3429 714.6 0 3429 10 87 714.6 0 87 11 126 714.6 0 126 12 91 714.6 0 91 13 99 714.6 0 99 14 119 714.6 0 119 15 104 714.6 0 104 16 92 714.6 0 92 17 122 714.6 0 122 18 82 714.6 0 82 19 91 714.6 0 91 20 113 714.6 0 113 21 112 714.6 0 112 22 122 714.6 0 122 23 107 714.6 0 107 24 96 714.6 0 96 25 196 714.6 0 196 26 236 714.6 0 236 27 94 714.6 0 94 28 89 714.6 0 89 29 101 714.6 0 101 30 100 714.6 0 100 31 65 714.6 0 65 32 103 714.6 0 103 33 106 714.6 0 106 34 78 714.6 0 78 35 90 714.6 0 90 36 118 714.6 0 118 37 87 714.6 0 87 38 103 714.6 0 103 39 93 714.6 0 93 40 71 714.6 0 71 41 115 714.6 0 115 42 87 714.6 0 87 43 82 714.6 0 82 44 116 714.6 0 116 45 96 714.6 0 96 46 91 714.6 0 91 47 82 714.6 0 82 48 83 714.6 0 83 49 87 714.6 0 87 50 103 714.6 0 103 51 148 714.6 0 148 52 96 714.6 0 96 53 122 714.6 0 122 54 76 714.6 0 76 55 85 714.6 0 85 56 105 714.6 0 105 57 82 714.6 0 82 58 95 714.6 0 95 59 101 714.6 0 101 60 105 714.6 0 105 61 242 714.6 0 242 62 113 714.6 0 113 63 308 714.6 0 308 64 67 714.6 0 67 65 93 714.6 0 93 66 96 714.6 0 96 67 91 714.6 0 91 68 79 714.6 0 79 69 110 714.6 0 110 70 118 714.6 0 118 71 205 714.6 0 205 72 94 714.6 0 94 73 70 714.6 0 70 74 123 714.6 0 123 75 98 714.6 0 98 76 103 714.6 0 103 77 193 714.6 0 193 78 359 714.6 0 359 79 227 714.6 0 227 80 412 714.6 0 412 81 563 714.6 0 563 82 767 714.6 0 767 83 1352 714.6 0 1352 84 629 714.6 0 629 85 273 714.6 0 273 86 187 714.6 0 187 87 159 714.6 0 159 88 176 714.6 0 176 89 195 714.6 0 195 90 137 714.6 0 137 91 311 714.6 0 311 92 417 714.6 0 417 93 381 714.6 0 381 94 668 714.6 0 668 95 455 714.6 0 455 96 280 714.6 0 280 97 471 714.6 0 471 98 336 714.6 0 336 99 460 714.6 0 460 100 453 714.6 0 453 101 504 714.6 0 504 102 398 714.6 0 398 103 239 714.6 0 239 104 194 714.6 0 194 105 280 714.6 0 280 106 250 714.6 0 250 107 261 714.6 0 261 108 328 714.6 0 328 109 512 714.6 0 512 110 545 714.6 0 545 111 515 714.6 0 515 112 3971 714.6 0 3971 113 5673 714.6 0 5673 114 9021 714.6 0 9021 115 420 714.6 0 420 116 473 714.6 0 473 117 665 714.6 0 665 118 792 714.6 0 792 119 1019 714.6 0 1019 120 1082 714.6 0 1082 121 1447 714.6 0 1447 122 1718 714.6 0 1718 123 477 714.6 0 477 124 500 714.6 0 500 125 672 714.6 0 672 126 557 714.6 0 557 127 864 714.6 0 864 128 522 714.6 0 522 129 491 714.6 0 491 130 512 714.6 0 512 131 653 714.6 0 653 132 553 714.6 0 553 133 510 714.6 0 510 134 605 714.6 0 605 135 607 714.6 0 607 136 1016 714.6 0 1016 137 636 714.6 0 636 138 590 714.6 0 590 139 798 714.6 0 798 140 622 714.6 0 622 141 570 714.6 0 570 142 845 714.6 0 845 143 1080 714.6 0 1080 144 928 714.6 0 928 145 680 714.6 0 680 146 631 714.6 0 631 147 696 714.6 0 696 148 744 714.6 0 744 149 755 714.6 0 755 150 579 714.6 0 579 151 690 714.6 0 690 152 767 714.6 0 767 153 606 714.6 0 606 154 745 714.6 0 745 155 673 714.6 0 673 156 566 714.6 0 566 157 697 714.6 0 697 158 601 714.6 0 601 159 596 714.6 0 596 160 555 714.6 0 555 161 691 714.6 0 691 162 579 714.6 0 579 163 664 714.6 0 664 164 741 714.6 0 741 165 669 714.6 0 669 166 1076 714.6 0 1076 167 1027 714.6 0 1027 168 847 714.6 0 847 169 1030 714.6 0 1030 170 911 714.6 0 911 171 1542 714.6 0 1542 172 2180 714.6 0 2180 173 1670 714.6 0 1670 174 932 714.6 0 932 175 982 714.6 0 982 176 750 714.6 0 750 177 853 714.6 0 853 178 778 714.6 0 778 179 764 714.6 0 764 180 856 714.6 0 856 181 798 714.6 0 798 182 819 714.6 0 819 183 1982 714.6 0 1982 184 2345 714.6 0 2345 185 1279 714.6 0 1279 186 692 714.6 0 692 187 726 714.6 0 726 188 741 714.6 0 741 189 642 714.6 0 642 190 848 714.6 0 848 191 1949 714.6 0 1949 192 1661 714.6 0 1661 193 1378 714.6 0 1378 194 1104 714.6 0 1104 195 803 714.6 0 803 196 691 714.6 0 691 197 997 714.6 0 997 198 867 714.6 0 867 199 1596 714.6 0 1596 200 783 714.6 0 783 201 614 714.6 0 614 202 654 714.6 0 654 203 833 714.6 0 833 204 665 714.6 0 665 205 623 714.6 0 623 206 609 714.6 0 609 207 594 714.6 0 594 208 616 714.6 0 616 209 584 714.6 0 584 210 609 714.6 0 609 211 756 714.6 0 756 212 669 714.6 0 669 213 663 714.6 0 663 214 641 714.6 0 641 215 816 714.6 0 816 216 917 714.6 0 917 217 694 714.6 0 694 218 792 714.6 0 792 219 808 714.6 0 808 220 756 714.6 0 756 221 538 714.6 0 538 222 527 714.6 0 527 223 762 714.6 0 762 224 716 714.6 0 716 225 733 714.6 0 733 226 619 714.6 0 619 227 657 714.6 0 657 228 835 714.6 0 835 229 796 714.6 0 796 230 673 714.6 0 673 231 583 714.6 0 583 232 630 714.6 0 630 233 590 714.6 0 590 234 648 714.6 0 648 235 858 714.6 0 858 236 762 714.6 0 762 237 632 714.6 0 632 238 573 714.6 0 573 239 657 714.6 0 657 240 612 714.6 0 612 241 472 714.6 0 472 242 517 714.6 0 517 243 754 714.6 0 754 244 649 714.6 0 649 245 738 714.6 0 738 246 652 714.6 0 652 247 655 714.6 0 655 248 356 714.6 0 356 249 361 714.6 0 361 250 327 714.6 0 327 251 725 714.6 0 725 252 1737 714.6 0 1737 253 992 714.6 0 992 254 622 714.6 0 622 255 736 714.6 0 736 256 733 714.6 0 733 257 697 714.6 0 697 258 557 714.6 0 557 259 574 714.6 0 574 260 441 714.6 0 441 261 507 714.6 0 507 262 684 714.6 0 684 263 717 714.6 0 717 264 652 714.6 0 652 265 778 714.6 0 778 266 517 714.6 0 517 267 935 714.6 0 935 268 1481 714.6 0 1481 269 872 714.6 0 872 270 523 714.6 0 523 271 644 714.6 0 644 272 793 714.6 0 793 273 640 714.6 0 640 274 577 714.6 0 577 275 493 714.6 0 493 276 432 714.6 0 432 277 495 714.6 0 495 278 667 714.6 0 667 279 565 714.6 0 565 280 850 714.6 0 850 281 573 714.6 0 573 282 492 714.6 0 492 283 1013 714.6 0 1013 284 879 714.6 0 879 285 529 714.6 0 529 286 600 714.6 0 600 287 645 714.6 0 645 288 524 714.6 0 524 289 512 714.6 0 512 290 817 714.6 0 817 291 1060 714.6 0 1060 292 1036 714.6 0 1036 293 1027 714.6 0 1027 294 594 714.6 0 594 295 493 714.6 0 493 296 686 714.6 0 686 297 826 714.6 0 826 298 960 714.6 0 960 299 882 714.6 0 882 300 910 714.6 0 910 301 142 714.6 0 142 Finished in 6.83 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 184,671 Reads with adapters: 3,548 (1.9%) Reads that were too short: 70 (0.0%) Reads written (passing filters): 3,478 (1.9%) Total basepairs processed: 39,102,650 bp Total written (filtered): 947,059 bp (2.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3548 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 26 0.0 3 0 25 0 1 17 3357 0.0 3 3291 65 1 18 17 0.0 3 1 9 5 2 19 10 0.0 3 0 0 3 7 20 4 0.0 3 0 0 0 4 21 3 0.0 3 0 0 0 3 24 8 0.0 3 0 0 1 7 26 2 0.0 3 0 0 0 2 27 3 0.0 3 0 0 0 3 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 1 1 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 55 2 0.0 3 0 0 0 2 57 2 0.0 3 0 0 1 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 110 2 0.0 3 0 0 0 2 112 4 0.0 3 0 0 0 4 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 1 132 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 162 4 0.0 3 0 0 0 4 168 2 0.0 3 0 0 0 2 169 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 181 8 0.0 3 0 0 1 7 182 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 1 218 2 0.0 3 0 0 0 2 252 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 289 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 3,478 Reads with adapters: 320 (9.2%) Reads written (passing filters): 3,478 (100.0%) Total basepairs processed: 947,059 bp Total written (filtered): 946,096 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 320 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.6% C: 8.4% G: 32.8% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 317 54.3 0 317 4 3 13.6 0 3