This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.21 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 153,305 (5.2%) Reads that were too short: 62,175 (2.1%) Reads written (passing filters): 91,130 (3.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 15,499,775 bp (1.8%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 153305 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 968 714.6 0 968 7 6993 714.6 0 6993 8 3456 714.6 0 3456 9 58 714.6 0 58 10 21 714.6 0 21 11 17 714.6 0 17 12 18 714.6 0 18 13 20 714.6 0 20 14 22 714.6 0 22 15 17 714.6 0 17 16 21 714.6 0 21 17 15 714.6 0 15 18 16 714.6 0 16 19 25 714.6 0 25 20 22 714.6 0 22 21 15 714.6 0 15 22 12 714.6 0 12 23 17 714.6 0 17 24 17 714.6 0 17 25 23 714.6 0 23 26 20 714.6 0 20 27 54 714.6 0 54 28 17 714.6 0 17 29 15 714.6 0 15 30 24 714.6 0 24 31 16 714.6 0 16 32 29 714.6 0 29 33 14 714.6 0 14 34 28 714.6 0 28 35 27 714.6 0 27 36 39 714.6 0 39 37 30 714.6 0 30 38 76 714.6 0 76 39 27 714.6 0 27 40 34 714.6 0 34 41 12 714.6 0 12 42 47 714.6 0 47 43 12 714.6 0 12 44 17 714.6 0 17 45 18 714.6 0 18 46 28 714.6 0 28 47 16 714.6 0 16 48 19 714.6 0 19 49 18 714.6 0 18 50 24 714.6 0 24 51 13 714.6 0 13 52 15 714.6 0 15 53 112 714.6 0 112 54 117 714.6 0 117 55 28 714.6 0 28 56 32 714.6 0 32 57 338 714.6 0 338 58 17 714.6 0 17 59 9 714.6 0 9 60 28 714.6 0 28 61 19 714.6 0 19 62 13 714.6 0 13 63 26 714.6 0 26 64 13 714.6 0 13 65 21 714.6 0 21 66 90 714.6 0 90 67 53 714.6 0 53 68 13 714.6 0 13 69 21 714.6 0 21 70 17 714.6 0 17 71 27 714.6 0 27 72 26 714.6 0 26 73 54 714.6 0 54 74 44 714.6 0 44 75 36 714.6 0 36 76 46 714.6 0 46 77 42 714.6 0 42 78 81 714.6 0 81 79 49 714.6 0 49 80 109 714.6 0 109 81 121 714.6 0 121 82 166 714.6 0 166 83 144 714.6 0 144 84 125 714.6 0 125 85 178 714.6 0 178 86 210 714.6 0 210 87 227 714.6 0 227 88 246 714.6 0 246 89 307 714.6 0 307 90 216 714.6 0 216 91 452 714.6 0 452 92 221 714.6 0 221 93 565 714.6 0 565 94 474 714.6 0 474 95 223 714.6 0 223 96 57 714.6 0 57 97 104 714.6 0 104 98 112 714.6 0 112 99 80 714.6 0 80 100 67 714.6 0 67 101 205 714.6 0 205 102 250 714.6 0 250 103 433 714.6 0 433 104 358 714.6 0 358 105 400 714.6 0 400 106 208 714.6 0 208 107 451 714.6 0 451 108 156 714.6 0 156 109 216 714.6 0 216 110 157 714.6 0 157 111 773 714.6 0 773 112 658 714.6 0 658 113 853 714.6 0 853 114 670 714.6 0 670 115 398 714.6 0 398 116 211 714.6 0 211 117 185 714.6 0 185 118 154 714.6 0 154 119 203 714.6 0 203 120 315 714.6 0 315 121 245 714.6 0 245 122 208 714.6 0 208 123 277 714.6 0 277 124 350 714.6 0 350 125 1376 714.6 0 1376 126 2310 714.6 0 2310 127 1005 714.6 0 1005 128 298 714.6 0 298 129 315 714.6 0 315 130 485 714.6 0 485 131 269 714.6 0 269 132 507 714.6 0 507 133 519 714.6 0 519 134 640 714.6 0 640 135 405 714.6 0 405 136 290 714.6 0 290 137 459 714.6 0 459 138 585 714.6 0 585 139 601 714.6 0 601 140 570 714.6 0 570 141 484 714.6 0 484 142 288 714.6 0 288 143 374 714.6 0 374 144 920 714.6 0 920 145 920 714.6 0 920 146 711 714.6 0 711 147 526 714.6 0 526 148 390 714.6 0 390 149 449 714.6 0 449 150 979 714.6 0 979 151 1486 714.6 0 1486 152 789 714.6 0 789 153 601 714.6 0 601 154 677 714.6 0 677 155 1833 714.6 0 1833 156 1044 714.6 0 1044 157 1028 714.6 0 1028 158 1251 714.6 0 1251 159 1203 714.6 0 1203 160 6035 714.6 0 6035 161 4696 714.6 0 4696 162 2368 714.6 0 2368 163 615 714.6 0 615 164 1130 714.6 0 1130 165 1044 714.6 0 1044 166 762 714.6 0 762 167 435 714.6 0 435 168 508 714.6 0 508 169 652 714.6 0 652 170 547 714.6 0 547 171 431 714.6 0 431 172 470 714.6 0 470 173 510 714.6 0 510 174 441 714.6 0 441 175 475 714.6 0 475 176 546 714.6 0 546 177 686 714.6 0 686 178 692 714.6 0 692 179 721 714.6 0 721 180 1771 714.6 0 1771 181 1733 714.6 0 1733 182 1614 714.6 0 1614 183 534 714.6 0 534 184 519 714.6 0 519 185 579 714.6 0 579 186 843 714.6 0 843 187 472 714.6 0 472 188 475 714.6 0 475 189 659 714.6 0 659 190 625 714.6 0 625 191 619 714.6 0 619 192 360 714.6 0 360 193 280 714.6 0 280 194 202 714.6 0 202 195 307 714.6 0 307 196 403 714.6 0 403 197 415 714.6 0 415 198 350 714.6 0 350 199 395 714.6 0 395 200 429 714.6 0 429 201 623 714.6 0 623 202 424 714.6 0 424 203 287 714.6 0 287 204 338 714.6 0 338 205 369 714.6 0 369 206 426 714.6 0 426 207 537 714.6 0 537 208 633 714.6 0 633 209 471 714.6 0 471 210 505 714.6 0 505 211 689 714.6 0 689 212 1446 714.6 0 1446 213 1593 714.6 0 1593 214 2428 714.6 0 2428 215 562 714.6 0 562 216 725 714.6 0 725 217 718 714.6 0 718 218 661 714.6 0 661 219 418 714.6 0 418 220 586 714.6 0 586 221 672 714.6 0 672 222 630 714.6 0 630 223 548 714.6 0 548 224 711 714.6 0 711 225 1009 714.6 0 1009 226 741 714.6 0 741 227 738 714.6 0 738 228 558 714.6 0 558 229 580 714.6 0 580 230 565 714.6 0 565 231 539 714.6 0 539 232 424 714.6 0 424 233 517 714.6 0 517 234 552 714.6 0 552 235 345 714.6 0 345 236 394 714.6 0 394 237 565 714.6 0 565 238 525 714.6 0 525 239 508 714.6 0 508 240 554 714.6 0 554 241 878 714.6 0 878 242 667 714.6 0 667 243 747 714.6 0 747 244 698 714.6 0 698 245 841 714.6 0 841 246 777 714.6 0 777 247 693 714.6 0 693 248 488 714.6 0 488 249 431 714.6 0 431 250 601 714.6 0 601 251 492 714.6 0 492 252 437 714.6 0 437 253 492 714.6 0 492 254 964 714.6 0 964 255 1063 714.6 0 1063 256 1581 714.6 0 1581 257 995 714.6 0 995 258 447 714.6 0 447 259 336 714.6 0 336 260 472 714.6 0 472 261 439 714.6 0 439 262 453 714.6 0 453 263 427 714.6 0 427 264 585 714.6 0 585 265 558 714.6 0 558 266 1260 714.6 0 1260 267 801 714.6 0 801 268 607 714.6 0 607 269 569 714.6 0 569 270 650 714.6 0 650 271 584 714.6 0 584 272 616 714.6 0 616 273 1055 714.6 0 1055 274 710 714.6 0 710 275 518 714.6 0 518 276 1658 714.6 0 1658 277 924 714.6 0 924 278 521 714.6 0 521 279 395 714.6 0 395 280 600 714.6 0 600 281 649 714.6 0 649 282 1122 714.6 0 1122 283 650 714.6 0 650 284 792 714.6 0 792 285 451 714.6 0 451 286 265 714.6 0 265 287 523 714.6 0 523 288 650 714.6 0 650 289 601 714.6 0 601 290 323 714.6 0 323 291 381 714.6 0 381 292 208 714.6 0 208 293 237 714.6 0 237 294 250 714.6 0 250 295 216 714.6 0 216 296 209 714.6 0 209 297 211 714.6 0 211 298 295 714.6 0 295 299 204 714.6 0 204 300 226 714.6 0 226 301 191 714.6 0 191 Finished in 3.37 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 91,130 Reads with adapters: 7,984 (8.8%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 7,942 (8.7%) Total basepairs processed: 15,499,775 bp Total written (filtered): 2,173,618 bp (14.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7984 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 2 1 1 19 108 0.0 3 0 105 2 1 20 7206 0.0 4 7045 142 13 4 2 21 586 0.0 4 555 27 1 2 1 22 10 0.0 4 1 3 5 0 1 23 5 0.0 4 0 0 0 5 24 4 0.0 4 1 0 0 1 2 26 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 57 2 0.0 4 0 0 0 0 2 60 3 0.0 4 0 0 0 0 3 61 1 0.0 4 0 0 0 0 1 63 3 0.0 4 0 0 0 0 3 71 2 0.0 4 0 0 0 1 1 77 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 1 1 97 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 103 2 0.0 4 0 0 0 0 2 105 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 111 4 0.0 4 0 0 0 4 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 128 5 0.0 4 0 0 0 0 5 130 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 163 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 251 2 0.0 4 0 0 0 0 2 252 1 0.0 4 0 0 0 0 1 253 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 7,942 Reads with adapters: 256 (3.2%) Reads written (passing filters): 7,942 (100.0%) Total basepairs processed: 2,173,618 bp Total written (filtered): 2,167,383 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 256 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.7% C: 5.5% G: 81.6% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 124.1 0 8 10 2 0.0 1 0 2 11 215 0.0 1 1 214 12 13 0.0 1 0 13 13 1 0.0 1 0 1 152 1 0.0 1 1 191 1 0.0 1 0 1 209 1 0.0 1 1 210 10 0.0 1 9 1 211 1 0.0 1 1 261 2 0.0 1 2 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.