This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 69,309 (2.4%) Reads that were too short: 42,390 (1.4%) Reads written (passing filters): 26,919 (0.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 4,836,241 bp (0.5%) === Adapter 1 === Sequence: AACGCG; Type: regular 5'; Length: 6; Trimmed: 69309 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 88 714.6 0 88 7 83 714.6 0 83 8 49 714.6 0 49 9 92 714.6 0 92 10 80 714.6 0 80 11 45 714.6 0 45 12 110 714.6 0 110 13 63 714.6 0 63 14 54 714.6 0 54 15 59 714.6 0 59 16 76 714.6 0 76 17 63 714.6 0 63 18 80 714.6 0 80 19 44 714.6 0 44 20 63 714.6 0 63 21 59 714.6 0 59 22 50 714.6 0 50 23 54 714.6 0 54 24 47 714.6 0 47 25 66 714.6 0 66 26 54 714.6 0 54 27 52 714.6 0 52 28 102 714.6 0 102 29 68 714.6 0 68 30 65 714.6 0 65 31 65 714.6 0 65 32 63 714.6 0 63 33 53 714.6 0 53 34 67 714.6 0 67 35 60 714.6 0 60 36 42 714.6 0 42 37 67 714.6 0 67 38 59 714.6 0 59 39 255 714.6 0 255 40 58 714.6 0 58 41 69 714.6 0 69 42 67 714.6 0 67 43 52 714.6 0 52 44 66 714.6 0 66 45 53 714.6 0 53 46 88 714.6 0 88 47 75 714.6 0 75 48 58 714.6 0 58 49 54 714.6 0 54 50 47 714.6 0 47 51 51 714.6 0 51 52 55 714.6 0 55 53 62 714.6 0 62 54 58 714.6 0 58 55 56 714.6 0 56 56 45 714.6 0 45 57 45 714.6 0 45 58 35 714.6 0 35 59 55 714.6 0 55 60 61 714.6 0 61 61 48 714.6 0 48 62 56 714.6 0 56 63 58 714.6 0 58 64 47 714.6 0 47 65 40 714.6 0 40 66 38 714.6 0 38 67 52 714.6 0 52 68 49 714.6 0 49 69 55 714.6 0 55 70 75 714.6 0 75 71 1177 714.6 0 1177 72 691 714.6 0 691 73 550 714.6 0 550 74 70 714.6 0 70 75 88 714.6 0 88 76 80 714.6 0 80 77 125 714.6 0 125 78 105 714.6 0 105 79 165 714.6 0 165 80 114 714.6 0 114 81 162 714.6 0 162 82 244 714.6 0 244 83 194 714.6 0 194 84 251 714.6 0 251 85 148 714.6 0 148 86 165 714.6 0 165 87 54 714.6 0 54 88 107 714.6 0 107 89 112 714.6 0 112 90 167 714.6 0 167 91 230 714.6 0 230 92 267 714.6 0 267 93 134 714.6 0 134 94 160 714.6 0 160 95 73 714.6 0 73 96 96 714.6 0 96 97 91 714.6 0 91 98 77 714.6 0 77 99 86 714.6 0 86 100 91 714.6 0 91 101 89 714.6 0 89 102 80 714.6 0 80 103 56 714.6 0 56 104 59 714.6 0 59 105 45 714.6 0 45 106 60 714.6 0 60 107 64 714.6 0 64 108 87 714.6 0 87 109 116 714.6 0 116 110 117 714.6 0 117 111 125 714.6 0 125 112 116 714.6 0 116 113 138 714.6 0 138 114 135 714.6 0 135 115 147 714.6 0 147 116 115 714.6 0 115 117 155 714.6 0 155 118 188 714.6 0 188 119 145 714.6 0 145 120 118 714.6 0 118 121 147 714.6 0 147 122 182 714.6 0 182 123 184 714.6 0 184 124 295 714.6 0 295 125 242 714.6 0 242 126 139 714.6 0 139 127 199 714.6 0 199 128 233 714.6 0 233 129 191 714.6 0 191 130 127 714.6 0 127 131 146 714.6 0 146 132 129 714.6 0 129 133 135 714.6 0 135 134 174 714.6 0 174 135 214 714.6 0 214 136 198 714.6 0 198 137 138 714.6 0 138 138 113 714.6 0 113 139 138 714.6 0 138 140 139 714.6 0 139 141 138 714.6 0 138 142 122 714.6 0 122 143 149 714.6 0 149 144 161 714.6 0 161 145 143 714.6 0 143 146 137 714.6 0 137 147 108 714.6 0 108 148 128 714.6 0 128 149 137 714.6 0 137 150 142 714.6 0 142 151 131 714.6 0 131 152 134 714.6 0 134 153 132 714.6 0 132 154 150 714.6 0 150 155 143 714.6 0 143 156 123 714.6 0 123 157 153 714.6 0 153 158 134 714.6 0 134 159 179 714.6 0 179 160 123 714.6 0 123 161 128 714.6 0 128 162 123 714.6 0 123 163 171 714.6 0 171 164 194 714.6 0 194 165 163 714.6 0 163 166 163 714.6 0 163 167 150 714.6 0 150 168 132 714.6 0 132 169 163 714.6 0 163 170 167 714.6 0 167 171 261 714.6 0 261 172 190 714.6 0 190 173 185 714.6 0 185 174 163 714.6 0 163 175 136 714.6 0 136 176 167 714.6 0 167 177 213 714.6 0 213 178 236 714.6 0 236 179 241 714.6 0 241 180 237 714.6 0 237 181 187 714.6 0 187 182 405 714.6 0 405 183 336 714.6 0 336 184 269 714.6 0 269 185 146 714.6 0 146 186 136 714.6 0 136 187 108 714.6 0 108 188 236 714.6 0 236 189 362 714.6 0 362 190 335 714.6 0 335 191 247 714.6 0 247 192 211 714.6 0 211 193 118 714.6 0 118 194 102 714.6 0 102 195 128 714.6 0 128 196 235 714.6 0 235 197 479 714.6 0 479 198 400 714.6 0 400 199 138 714.6 0 138 200 77 714.6 0 77 201 97 714.6 0 97 202 190 714.6 0 190 203 235 714.6 0 235 204 206 714.6 0 206 205 144 714.6 0 144 206 103 714.6 0 103 207 133 714.6 0 133 208 126 714.6 0 126 209 101 714.6 0 101 210 117 714.6 0 117 211 160 714.6 0 160 212 162 714.6 0 162 213 166 714.6 0 166 214 165 714.6 0 165 215 171 714.6 0 171 216 174 714.6 0 174 217 287 714.6 0 287 218 288 714.6 0 288 219 363 714.6 0 363 220 626 714.6 0 626 221 1016 714.6 0 1016 222 718 714.6 0 718 223 445 714.6 0 445 224 300 714.6 0 300 225 329 714.6 0 329 226 301 714.6 0 301 227 262 714.6 0 262 228 267 714.6 0 267 229 151 714.6 0 151 230 135 714.6 0 135 231 134 714.6 0 134 232 146 714.6 0 146 233 150 714.6 0 150 234 222 714.6 0 222 235 217 714.6 0 217 236 330 714.6 0 330 237 207 714.6 0 207 238 202 714.6 0 202 239 173 714.6 0 173 240 168 714.6 0 168 241 150 714.6 0 150 242 283 714.6 0 283 243 265 714.6 0 265 244 1210 714.6 0 1210 245 1498 714.6 0 1498 246 1930 714.6 0 1930 247 553 714.6 0 553 248 226 714.6 0 226 249 124 714.6 0 124 250 173 714.6 0 173 251 246 714.6 0 246 252 310 714.6 0 310 253 290 714.6 0 290 254 200 714.6 0 200 255 248 714.6 0 248 256 193 714.6 0 193 257 100 714.6 0 100 258 49 714.6 0 49 259 61 714.6 0 61 260 65 714.6 0 65 261 146 714.6 0 146 262 177 714.6 0 177 263 247 714.6 0 247 264 270 714.6 0 270 265 264 714.6 0 264 266 189 714.6 0 189 267 270 714.6 0 270 268 256 714.6 0 256 269 205 714.6 0 205 270 207 714.6 0 207 271 249 714.6 0 249 272 192 714.6 0 192 273 133 714.6 0 133 274 107 714.6 0 107 275 100 714.6 0 100 276 80 714.6 0 80 277 219 714.6 0 219 278 226 714.6 0 226 279 167 714.6 0 167 280 71 714.6 0 71 281 95 714.6 0 95 282 149 714.6 0 149 283 362 714.6 0 362 284 295 714.6 0 295 285 275 714.6 0 275 286 280 714.6 0 280 287 468 714.6 0 468 288 2194 714.6 0 2194 289 8593 714.6 0 8593 290 6240 714.6 0 6240 291 771 714.6 0 771 292 156 714.6 0 156 293 80 714.6 0 80 294 85 714.6 0 85 295 113 714.6 0 113 296 153 714.6 0 153 297 88 714.6 0 88 298 86 714.6 0 86 299 77 714.6 0 77 300 67 714.6 0 67 301 24 714.6 0 24 Finished in 1.07 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 26,919 Reads with adapters: 49 (0.2%) Reads that were too short: 25 (0.1%) Reads written (passing filters): 24 (0.1%) Total basepairs processed: 4,836,241 bp Total written (filtered): 5,266 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 49 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 4 0.0 4 4 21 7 0.0 4 6 0 0 0 1 22 3 0.0 4 0 2 0 0 1 28 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 1 106 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 117 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 0 2 146 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 166 3 0.0 4 0 0 0 0 3 167 2 0.0 4 0 0 0 0 2 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (417 us/read; 0.14 M reads/minute). === Summary === Total reads processed: 24 Reads with adapters: 0 (0.0%) Reads written (passing filters): 24 (100.0%) Total basepairs processed: 5,266 bp Total written (filtered): 5,266 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.