This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.59 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 66,449 (2.3%) Reads that were too short: 21,211 (0.7%) Reads written (passing filters): 45,238 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 6,352,577 bp (0.7%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 66449 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 175 714.6 0 175 7 29 714.6 0 29 8 115 714.6 0 115 9 1578 714.6 0 1578 10 13 714.6 0 13 11 17 714.6 0 17 12 21 714.6 0 21 13 13 714.6 0 13 14 19 714.6 0 19 15 14 714.6 0 14 16 16 714.6 0 16 17 20 714.6 0 20 18 16 714.6 0 16 19 20 714.6 0 20 20 15 714.6 0 15 21 27 714.6 0 27 22 26 714.6 0 26 23 19 714.6 0 19 24 12 714.6 0 12 25 14 714.6 0 14 26 21 714.6 0 21 27 12 714.6 0 12 28 23 714.6 0 23 29 28 714.6 0 28 30 25 714.6 0 25 31 19 714.6 0 19 32 55 714.6 0 55 33 52 714.6 0 52 34 17 714.6 0 17 35 13 714.6 0 13 36 11 714.6 0 11 37 23 714.6 0 23 38 25 714.6 0 25 39 26 714.6 0 26 40 25 714.6 0 25 41 23 714.6 0 23 42 18 714.6 0 18 43 22 714.6 0 22 44 27 714.6 0 27 45 18 714.6 0 18 46 16 714.6 0 16 47 29 714.6 0 29 48 20 714.6 0 20 49 24 714.6 0 24 50 19 714.6 0 19 51 22 714.6 0 22 52 16 714.6 0 16 53 18 714.6 0 18 54 23 714.6 0 23 55 26 714.6 0 26 56 15 714.6 0 15 57 24 714.6 0 24 58 17 714.6 0 17 59 17 714.6 0 17 60 21 714.6 0 21 61 16 714.6 0 16 62 20 714.6 0 20 63 11 714.6 0 11 64 18 714.6 0 18 65 21 714.6 0 21 66 25 714.6 0 25 67 19 714.6 0 19 68 22 714.6 0 22 69 91 714.6 0 91 70 22 714.6 0 22 71 27 714.6 0 27 72 13 714.6 0 13 73 20 714.6 0 20 74 27 714.6 0 27 75 16 714.6 0 16 76 21 714.6 0 21 77 21 714.6 0 21 78 14 714.6 0 14 79 26 714.6 0 26 80 86 714.6 0 86 81 41 714.6 0 41 82 35 714.6 0 35 83 21 714.6 0 21 84 23 714.6 0 23 85 23 714.6 0 23 86 24 714.6 0 24 87 21 714.6 0 21 88 37 714.6 0 37 89 18 714.6 0 18 90 20 714.6 0 20 91 17 714.6 0 17 92 15 714.6 0 15 93 22 714.6 0 22 94 28 714.6 0 28 95 48 714.6 0 48 96 123 714.6 0 123 97 251 714.6 0 251 98 73 714.6 0 73 99 49 714.6 0 49 100 59 714.6 0 59 101 59 714.6 0 59 102 43 714.6 0 43 103 46 714.6 0 46 104 34 714.6 0 34 105 33 714.6 0 33 106 16 714.6 0 16 107 37 714.6 0 37 108 233 714.6 0 233 109 18 714.6 0 18 110 28 714.6 0 28 111 44 714.6 0 44 112 34 714.6 0 34 113 61 714.6 0 61 114 67 714.6 0 67 115 42 714.6 0 42 116 43 714.6 0 43 117 369 714.6 0 369 118 270 714.6 0 270 119 236 714.6 0 236 120 54 714.6 0 54 121 39 714.6 0 39 122 64 714.6 0 64 123 42 714.6 0 42 124 45 714.6 0 45 125 55 714.6 0 55 126 66 714.6 0 66 127 102 714.6 0 102 128 90 714.6 0 90 129 72 714.6 0 72 130 67 714.6 0 67 131 157 714.6 0 157 132 97 714.6 0 97 133 179 714.6 0 179 134 119 714.6 0 119 135 90 714.6 0 90 136 135 714.6 0 135 137 103 714.6 0 103 138 88 714.6 0 88 139 115 714.6 0 115 140 107 714.6 0 107 141 84 714.6 0 84 142 67 714.6 0 67 143 74 714.6 0 74 144 59 714.6 0 59 145 47 714.6 0 47 146 91 714.6 0 91 147 101 714.6 0 101 148 65 714.6 0 65 149 54 714.6 0 54 150 50 714.6 0 50 151 37 714.6 0 37 152 68 714.6 0 68 153 46 714.6 0 46 154 56 714.6 0 56 155 73 714.6 0 73 156 195 714.6 0 195 157 151 714.6 0 151 158 312 714.6 0 312 159 86 714.6 0 86 160 79 714.6 0 79 161 145 714.6 0 145 162 111 714.6 0 111 163 127 714.6 0 127 164 138 714.6 0 138 165 277 714.6 0 277 166 176 714.6 0 176 167 168 714.6 0 168 168 207 714.6 0 207 169 231 714.6 0 231 170 165 714.6 0 165 171 201 714.6 0 201 172 175 714.6 0 175 173 158 714.6 0 158 174 201 714.6 0 201 175 411 714.6 0 411 176 538 714.6 0 538 177 1060 714.6 0 1060 178 2164 714.6 0 2164 179 18903 714.6 0 18903 180 1416 714.6 0 1416 181 1691 714.6 0 1691 182 1332 714.6 0 1332 183 404 714.6 0 404 184 2014 714.6 0 2014 185 143 714.6 0 143 186 86 714.6 0 86 187 110 714.6 0 110 188 109 714.6 0 109 189 89 714.6 0 89 190 80 714.6 0 80 191 60 714.6 0 60 192 67 714.6 0 67 193 94 714.6 0 94 194 196 714.6 0 196 195 317 714.6 0 317 196 523 714.6 0 523 197 356 714.6 0 356 198 274 714.6 0 274 199 92 714.6 0 92 200 172 714.6 0 172 201 210 714.6 0 210 202 265 714.6 0 265 203 195 714.6 0 195 204 125 714.6 0 125 205 82 714.6 0 82 206 57 714.6 0 57 207 77 714.6 0 77 208 103 714.6 0 103 209 76 714.6 0 76 210 50 714.6 0 50 211 60 714.6 0 60 212 74 714.6 0 74 213 120 714.6 0 120 214 111 714.6 0 111 215 121 714.6 0 121 216 112 714.6 0 112 217 111 714.6 0 111 218 53 714.6 0 53 219 85 714.6 0 85 220 86 714.6 0 86 221 96 714.6 0 96 222 59 714.6 0 59 223 78 714.6 0 78 224 119 714.6 0 119 225 127 714.6 0 127 226 84 714.6 0 84 227 70 714.6 0 70 228 95 714.6 0 95 229 153 714.6 0 153 230 86 714.6 0 86 231 68 714.6 0 68 232 66 714.6 0 66 233 63 714.6 0 63 234 92 714.6 0 92 235 90 714.6 0 90 236 110 714.6 0 110 237 97 714.6 0 97 238 180 714.6 0 180 239 1492 714.6 0 1492 240 311 714.6 0 311 241 526 714.6 0 526 242 75 714.6 0 75 243 91 714.6 0 91 244 70 714.6 0 70 245 93 714.6 0 93 246 92 714.6 0 92 247 73 714.6 0 73 248 125 714.6 0 125 249 148 714.6 0 148 250 109 714.6 0 109 251 61 714.6 0 61 252 67 714.6 0 67 253 93 714.6 0 93 254 103 714.6 0 103 255 78 714.6 0 78 256 103 714.6 0 103 257 147 714.6 0 147 258 204 714.6 0 204 259 505 714.6 0 505 260 794 714.6 0 794 261 979 714.6 0 979 262 156 714.6 0 156 263 420 714.6 0 420 264 971 714.6 0 971 265 1213 714.6 0 1213 266 1264 714.6 0 1264 267 845 714.6 0 845 268 108 714.6 0 108 269 68 714.6 0 68 270 54 714.6 0 54 271 26 714.6 0 26 272 32 714.6 0 32 273 41 714.6 0 41 274 62 714.6 0 62 275 99 714.6 0 99 276 76 714.6 0 76 277 65 714.6 0 65 278 58 714.6 0 58 279 93 714.6 0 93 280 100 714.6 0 100 281 102 714.6 0 102 282 57 714.6 0 57 283 53 714.6 0 53 284 49 714.6 0 49 285 52 714.6 0 52 286 63 714.6 0 63 287 106 714.6 0 106 288 149 714.6 0 149 289 105 714.6 0 105 290 51 714.6 0 51 291 215 714.6 0 215 292 796 714.6 0 796 293 1388 714.6 0 1388 294 849 714.6 0 849 295 836 714.6 0 836 296 199 714.6 0 199 297 98 714.6 0 98 298 46 714.6 0 46 299 73 714.6 0 73 300 93 714.6 0 93 301 45 714.6 0 45 Finished in 1.37 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 45,238 Reads with adapters: 200 (0.4%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 181 (0.4%) Total basepairs processed: 6,352,577 bp Total written (filtered): 47,440 bp (0.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 200 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 8 1 0 1 18 1 0.0 3 0 0 1 20 156 0.0 3 151 5 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 181 Reads with adapters: 12 (6.6%) Reads written (passing filters): 181 (100.0%) Total basepairs processed: 47,440 bp Total written (filtered): 47,404 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 12 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 8.3% G: 58.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12 2.8 0 12