This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCTAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 88.93 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 126,116 (3.6%) Reads that were too short: 72,271 (2.1%) Reads written (passing filters): 53,845 (1.6%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 9,114,306 bp (0.9%) === Adapter 1 === Sequence: GGCTAC; Type: regular 5'; Length: 6; Trimmed: 126116 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 29 846.0 0 29 7 61 846.0 0 61 8 41 846.0 0 41 9 27 846.0 0 27 10 20 846.0 0 20 11 20 846.0 0 20 12 53 846.0 0 53 13 119 846.0 0 119 14 438 846.0 0 438 15 243 846.0 0 243 16 51 846.0 0 51 17 20 846.0 0 20 18 10 846.0 0 10 19 8 846.0 0 8 20 22 846.0 0 22 21 9 846.0 0 9 22 19 846.0 0 19 23 20 846.0 0 20 24 18 846.0 0 18 25 8 846.0 0 8 26 11 846.0 0 11 27 12 846.0 0 12 28 13 846.0 0 13 29 51 846.0 0 51 30 22 846.0 0 22 31 45 846.0 0 45 32 21 846.0 0 21 33 14 846.0 0 14 34 9 846.0 0 9 35 20 846.0 0 20 36 20 846.0 0 20 37 27 846.0 0 27 38 18 846.0 0 18 39 33 846.0 0 33 40 65 846.0 0 65 41 34 846.0 0 34 42 20 846.0 0 20 43 18 846.0 0 18 44 19 846.0 0 19 45 23 846.0 0 23 46 19 846.0 0 19 47 9 846.0 0 9 48 22 846.0 0 22 49 20 846.0 0 20 50 59 846.0 0 59 51 129 846.0 0 129 52 22 846.0 0 22 53 13 846.0 0 13 54 26 846.0 0 26 55 21 846.0 0 21 56 17 846.0 0 17 57 15 846.0 0 15 58 18 846.0 0 18 59 25 846.0 0 25 60 13 846.0 0 13 61 12 846.0 0 12 62 15 846.0 0 15 63 13 846.0 0 13 64 15 846.0 0 15 65 19 846.0 0 19 66 16 846.0 0 16 67 15 846.0 0 15 68 13 846.0 0 13 69 11 846.0 0 11 70 10 846.0 0 10 71 13 846.0 0 13 72 9 846.0 0 9 73 8 846.0 0 8 74 10 846.0 0 10 75 15 846.0 0 15 76 15 846.0 0 15 77 14 846.0 0 14 78 21 846.0 0 21 79 20 846.0 0 20 80 22 846.0 0 22 81 35 846.0 0 35 82 37 846.0 0 37 83 90 846.0 0 90 84 118 846.0 0 118 85 42 846.0 0 42 86 37 846.0 0 37 87 73 846.0 0 73 88 103 846.0 0 103 89 86 846.0 0 86 90 41 846.0 0 41 91 19 846.0 0 19 92 22 846.0 0 22 93 23 846.0 0 23 94 23 846.0 0 23 95 33 846.0 0 33 96 40 846.0 0 40 97 32 846.0 0 32 98 17 846.0 0 17 99 30 846.0 0 30 100 44 846.0 0 44 101 35 846.0 0 35 102 34 846.0 0 34 103 23 846.0 0 23 104 41 846.0 0 41 105 19 846.0 0 19 106 35 846.0 0 35 107 20 846.0 0 20 108 33 846.0 0 33 109 97 846.0 0 97 110 241 846.0 0 241 111 254 846.0 0 254 112 67 846.0 0 67 113 66 846.0 0 66 114 144 846.0 0 144 115 147 846.0 0 147 116 101 846.0 0 101 117 91 846.0 0 91 118 110 846.0 0 110 119 122 846.0 0 122 120 146 846.0 0 146 121 176 846.0 0 176 122 274 846.0 0 274 123 689 846.0 0 689 124 16932 846.0 0 16932 125 5174 846.0 0 5174 126 8185 846.0 0 8185 127 129 846.0 0 129 128 148 846.0 0 148 129 74 846.0 0 74 130 116 846.0 0 116 131 60 846.0 0 60 132 34 846.0 0 34 133 339 846.0 0 339 134 49 846.0 0 49 135 50 846.0 0 50 136 26 846.0 0 26 137 33 846.0 0 33 138 51 846.0 0 51 139 46 846.0 0 46 140 57 846.0 0 57 141 58 846.0 0 58 142 44 846.0 0 44 143 58 846.0 0 58 144 62 846.0 0 62 145 82 846.0 0 82 146 99 846.0 0 99 147 204 846.0 0 204 148 256 846.0 0 256 149 283 846.0 0 283 150 62 846.0 0 62 151 158 846.0 0 158 152 2436 846.0 0 2436 153 256 846.0 0 256 154 443 846.0 0 443 155 189 846.0 0 189 156 324 846.0 0 324 157 943 846.0 0 943 158 310 846.0 0 310 159 939 846.0 0 939 160 339 846.0 0 339 161 276 846.0 0 276 162 621 846.0 0 621 163 78 846.0 0 78 164 61 846.0 0 61 165 68 846.0 0 68 166 46 846.0 0 46 167 49 846.0 0 49 168 74 846.0 0 74 169 71 846.0 0 71 170 106 846.0 0 106 171 85 846.0 0 85 172 178 846.0 0 178 173 154 846.0 0 154 174 228 846.0 0 228 175 66 846.0 0 66 176 166 846.0 0 166 177 2941 846.0 0 2941 178 910 846.0 0 910 179 1038 846.0 0 1038 180 260 846.0 0 260 181 35 846.0 0 35 182 44 846.0 0 44 183 34 846.0 0 34 184 35 846.0 0 35 185 28 846.0 0 28 186 36 846.0 0 36 187 34 846.0 0 34 188 46 846.0 0 46 189 24 846.0 0 24 190 182 846.0 0 182 191 34 846.0 0 34 192 59 846.0 0 59 193 47 846.0 0 47 194 82 846.0 0 82 195 55 846.0 0 55 196 57 846.0 0 57 197 49 846.0 0 49 198 15 846.0 0 15 199 26 846.0 0 26 200 26 846.0 0 26 201 18 846.0 0 18 202 18 846.0 0 18 203 19 846.0 0 19 204 31 846.0 0 31 205 35 846.0 0 35 206 27 846.0 0 27 207 58 846.0 0 58 208 57 846.0 0 57 209 49 846.0 0 49 210 18 846.0 0 18 211 18 846.0 0 18 212 32 846.0 0 32 213 48 846.0 0 48 214 55 846.0 0 55 215 43 846.0 0 43 216 32 846.0 0 32 217 42 846.0 0 42 218 33 846.0 0 33 219 44 846.0 0 44 220 67 846.0 0 67 221 42 846.0 0 42 222 26 846.0 0 26 223 26 846.0 0 26 224 25 846.0 0 25 225 31 846.0 0 31 226 38 846.0 0 38 227 27 846.0 0 27 228 39 846.0 0 39 229 39 846.0 0 39 230 56 846.0 0 56 231 53 846.0 0 53 232 29 846.0 0 29 233 220 846.0 0 220 234 92 846.0 0 92 235 157 846.0 0 157 236 54 846.0 0 54 237 18 846.0 0 18 238 24 846.0 0 24 239 44 846.0 0 44 240 47 846.0 0 47 241 26 846.0 0 26 242 34 846.0 0 34 243 30 846.0 0 30 244 39 846.0 0 39 245 32 846.0 0 32 246 43 846.0 0 43 247 54 846.0 0 54 248 42 846.0 0 42 249 122 846.0 0 122 250 41 846.0 0 41 251 22 846.0 0 22 252 39 846.0 0 39 253 31 846.0 0 31 254 56 846.0 0 56 255 76 846.0 0 76 256 69 846.0 0 69 257 254 846.0 0 254 258 228 846.0 0 228 259 1487 846.0 0 1487 260 115 846.0 0 115 261 105 846.0 0 105 262 162 846.0 0 162 263 1015 846.0 0 1015 264 198 846.0 0 198 265 260 846.0 0 260 266 50 846.0 0 50 267 114 846.0 0 114 268 701 846.0 0 701 269 221 846.0 0 221 270 252 846.0 0 252 271 79 846.0 0 79 272 35 846.0 0 35 273 43 846.0 0 43 274 49 846.0 0 49 275 71 846.0 0 71 276 73 846.0 0 73 277 30 846.0 0 30 278 24 846.0 0 24 279 54 846.0 0 54 280 233 846.0 0 233 281 86 846.0 0 86 282 36 846.0 0 36 283 48 846.0 0 48 284 22 846.0 0 22 285 34 846.0 0 34 286 68 846.0 0 68 287 123 846.0 0 123 288 805 846.0 0 805 289 475 846.0 0 475 290 777 846.0 0 777 291 243 846.0 0 243 292 1037 846.0 0 1037 293 12104 846.0 0 12104 294 33297 846.0 0 33297 295 13325 846.0 0 13325 296 1143 846.0 0 1143 297 76 846.0 0 76 298 41 846.0 0 41 299 56 846.0 0 56 300 17 846.0 0 17 301 36 846.0 0 36 Finished in 2.06 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 53,845 Reads with adapters: 42 (0.1%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 23 (0.0%) Total basepairs processed: 9,114,306 bp Total written (filtered): 5,826 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 1 0.0 3 0 0 1 20 18 0.0 4 11 6 1 23 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 63 3 0.0 4 0 0 0 0 3 64 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 1 1 73 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (435 us/read; 0.14 M reads/minute). === Summary === Total reads processed: 23 Reads with adapters: 0 (0.0%) Reads written (passing filters): 23 (100.0%) Total basepairs processed: 5,826 bp Total written (filtered): 5,826 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.