This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.69 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 483,534 (14.0%) Reads that were too short: 384,539 (11.1%) Reads written (passing filters): 98,995 (2.9%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 15,963,026 bp (1.5%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 483534 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 21 846.0 0 21 7 86 846.0 0 86 8 33 846.0 0 33 9 77 846.0 0 77 10 45 846.0 0 45 11 23 846.0 0 23 12 26 846.0 0 26 13 35 846.0 0 35 14 26 846.0 0 26 15 29 846.0 0 29 16 28 846.0 0 28 17 25 846.0 0 25 18 24 846.0 0 24 19 38 846.0 0 38 20 29 846.0 0 29 21 35 846.0 0 35 22 21 846.0 0 21 23 34 846.0 0 34 24 23 846.0 0 23 25 32 846.0 0 32 26 30 846.0 0 30 27 27 846.0 0 27 28 36 846.0 0 36 29 31 846.0 0 31 30 33 846.0 0 33 31 31 846.0 0 31 32 26 846.0 0 26 33 28 846.0 0 28 34 34 846.0 0 34 35 29 846.0 0 29 36 120 846.0 0 120 37 68 846.0 0 68 38 82 846.0 0 82 39 43 846.0 0 43 40 22 846.0 0 22 41 33 846.0 0 33 42 64 846.0 0 64 43 25 846.0 0 25 44 32 846.0 0 32 45 35 846.0 0 35 46 21 846.0 0 21 47 17 846.0 0 17 48 30 846.0 0 30 49 27 846.0 0 27 50 14 846.0 0 14 51 27 846.0 0 27 52 16 846.0 0 16 53 22 846.0 0 22 54 19 846.0 0 19 55 25 846.0 0 25 56 16 846.0 0 16 57 26 846.0 0 26 58 24 846.0 0 24 59 27 846.0 0 27 60 38 846.0 0 38 61 19 846.0 0 19 62 14 846.0 0 14 63 27 846.0 0 27 64 29 846.0 0 29 65 98 846.0 0 98 66 25 846.0 0 25 67 35 846.0 0 35 68 31 846.0 0 31 69 135 846.0 0 135 70 105 846.0 0 105 71 182 846.0 0 182 72 107 846.0 0 107 73 87 846.0 0 87 74 48 846.0 0 48 75 42 846.0 0 42 76 71 846.0 0 71 77 67 846.0 0 67 78 56 846.0 0 56 79 91 846.0 0 91 80 596 846.0 0 596 81 428 846.0 0 428 82 517 846.0 0 517 83 244 846.0 0 244 84 248 846.0 0 248 85 145 846.0 0 145 86 260 846.0 0 260 87 348 846.0 0 348 88 371 846.0 0 371 89 388 846.0 0 388 90 705 846.0 0 705 91 540 846.0 0 540 92 710 846.0 0 710 93 1031 846.0 0 1031 94 771 846.0 0 771 95 617 846.0 0 617 96 680 846.0 0 680 97 1181 846.0 0 1181 98 2827 846.0 0 2827 99 1691 846.0 0 1691 100 816 846.0 0 816 101 793 846.0 0 793 102 264 846.0 0 264 103 208 846.0 0 208 104 110 846.0 0 110 105 269 846.0 0 269 106 1373 846.0 0 1373 107 1005 846.0 0 1005 108 734 846.0 0 734 109 556 846.0 0 556 110 751 846.0 0 751 111 785 846.0 0 785 112 648 846.0 0 648 113 639 846.0 0 639 114 611 846.0 0 611 115 474 846.0 0 474 116 267 846.0 0 267 117 332 846.0 0 332 118 362 846.0 0 362 119 1038 846.0 0 1038 120 1028 846.0 0 1028 121 737 846.0 0 737 122 423 846.0 0 423 123 1369 846.0 0 1369 124 665 846.0 0 665 125 559 846.0 0 559 126 464 846.0 0 464 127 496 846.0 0 496 128 815 846.0 0 815 129 903 846.0 0 903 130 656 846.0 0 656 131 616 846.0 0 616 132 934 846.0 0 934 133 667 846.0 0 667 134 705 846.0 0 705 135 488 846.0 0 488 136 375 846.0 0 375 137 418 846.0 0 418 138 643 846.0 0 643 139 2372 846.0 0 2372 140 1707 846.0 0 1707 141 1084 846.0 0 1084 142 421 846.0 0 421 143 474 846.0 0 474 144 393 846.0 0 393 145 510 846.0 0 510 146 667 846.0 0 667 147 555 846.0 0 555 148 530 846.0 0 530 149 403 846.0 0 403 150 480 846.0 0 480 151 351 846.0 0 351 152 401 846.0 0 401 153 483 846.0 0 483 154 522 846.0 0 522 155 674 846.0 0 674 156 606 846.0 0 606 157 582 846.0 0 582 158 741 846.0 0 741 159 8566 846.0 0 8566 160 4839 846.0 0 4839 161 6377 846.0 0 6377 162 405 846.0 0 405 163 476 846.0 0 476 164 533 846.0 0 533 165 709 846.0 0 709 166 481 846.0 0 481 167 604 846.0 0 604 168 599 846.0 0 599 169 376 846.0 0 376 170 335 846.0 0 335 171 289 846.0 0 289 172 383 846.0 0 383 173 721 846.0 0 721 174 1228 846.0 0 1228 175 416 846.0 0 416 176 454 846.0 0 454 177 357 846.0 0 357 178 426 846.0 0 426 179 420 846.0 0 420 180 579 846.0 0 579 181 534 846.0 0 534 182 440 846.0 0 440 183 603 846.0 0 603 184 679 846.0 0 679 185 843 846.0 0 843 186 485 846.0 0 485 187 509 846.0 0 509 188 472 846.0 0 472 189 513 846.0 0 513 190 484 846.0 0 484 191 550 846.0 0 550 192 693 846.0 0 693 193 702 846.0 0 702 194 825 846.0 0 825 195 962 846.0 0 962 196 644 846.0 0 644 197 443 846.0 0 443 198 321 846.0 0 321 199 350 846.0 0 350 200 1019 846.0 0 1019 201 964 846.0 0 964 202 612 846.0 0 612 203 814 846.0 0 814 204 934 846.0 0 934 205 817 846.0 0 817 206 1246 846.0 0 1246 207 1728 846.0 0 1728 208 1395 846.0 0 1395 209 1192 846.0 0 1192 210 992 846.0 0 992 211 1115 846.0 0 1115 212 1139 846.0 0 1139 213 742 846.0 0 742 214 718 846.0 0 718 215 545 846.0 0 545 216 459 846.0 0 459 217 397 846.0 0 397 218 556 846.0 0 556 219 879 846.0 0 879 220 975 846.0 0 975 221 1203 846.0 0 1203 222 718 846.0 0 718 223 621 846.0 0 621 224 882 846.0 0 882 225 609 846.0 0 609 226 421 846.0 0 421 227 464 846.0 0 464 228 768 846.0 0 768 229 789 846.0 0 789 230 708 846.0 0 708 231 726 846.0 0 726 232 917 846.0 0 917 233 1176 846.0 0 1176 234 1176 846.0 0 1176 235 669 846.0 0 669 236 563 846.0 0 563 237 636 846.0 0 636 238 1021 846.0 0 1021 239 520 846.0 0 520 240 836 846.0 0 836 241 846 846.0 0 846 242 1088 846.0 0 1088 243 1051 846.0 0 1051 244 734 846.0 0 734 245 488 846.0 0 488 246 297 846.0 0 297 247 263 846.0 0 263 248 404 846.0 0 404 249 834 846.0 0 834 250 1595 846.0 0 1595 251 1740 846.0 0 1740 252 1969 846.0 0 1969 253 3488 846.0 0 3488 254 7675 846.0 0 7675 255 123579 846.0 0 123579 256 129709 846.0 0 129709 257 52746 846.0 0 52746 258 1731 846.0 0 1731 259 660 846.0 0 660 260 882 846.0 0 882 261 1197 846.0 0 1197 262 797 846.0 0 797 263 622 846.0 0 622 264 310 846.0 0 310 265 446 846.0 0 446 266 364 846.0 0 364 267 231 846.0 0 231 268 323 846.0 0 323 269 546 846.0 0 546 270 553 846.0 0 553 271 527 846.0 0 527 272 518 846.0 0 518 273 540 846.0 0 540 274 312 846.0 0 312 275 270 846.0 0 270 276 299 846.0 0 299 277 322 846.0 0 322 278 366 846.0 0 366 279 345 846.0 0 345 280 463 846.0 0 463 281 631 846.0 0 631 282 891 846.0 0 891 283 723 846.0 0 723 284 864 846.0 0 864 285 261 846.0 0 261 286 161 846.0 0 161 287 171 846.0 0 171 288 267 846.0 0 267 289 548 846.0 0 548 290 492 846.0 0 492 291 388 846.0 0 388 292 348 846.0 0 348 293 235 846.0 0 235 294 276 846.0 0 276 295 475 846.0 0 475 296 711 846.0 0 711 297 847 846.0 0 847 298 801 846.0 0 801 299 590 846.0 0 590 300 626 846.0 0 626 301 425 846.0 0 425 Finished in 3.15 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 98,995 Reads with adapters: 66 (0.1%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 41 (0.0%) Total basepairs processed: 15,963,026 bp Total written (filtered): 8,164 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 66 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 21 0.0 3 18 3 33 1 0.0 3 0 0 1 34 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 1 60 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (244 us/read; 0.25 M reads/minute). === Summary === Total reads processed: 41 Reads with adapters: 1 (2.4%) Reads written (passing filters): 41 (100.0%) Total basepairs processed: 8,164 bp Total written (filtered): 8,161 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.6 0 1