This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGTGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.51 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 77,647 (2.2%) Reads that were too short: 9,610 (0.3%) Reads written (passing filters): 68,037 (2.0%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 16,034,366 bp (1.5%) === Adapter 1 === Sequence: AGTGTT; Type: regular 5'; Length: 6; Trimmed: 77647 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 219 846.0 0 219 7 359 846.0 0 359 8 23935 846.0 0 23935 9 104 846.0 0 104 10 46 846.0 0 46 11 44 846.0 0 44 12 43 846.0 0 43 13 51 846.0 0 51 14 56 846.0 0 56 15 52 846.0 0 52 16 51 846.0 0 51 17 45 846.0 0 45 18 45 846.0 0 45 19 53 846.0 0 53 20 44 846.0 0 44 21 88 846.0 0 88 22 72 846.0 0 72 23 68 846.0 0 68 24 47 846.0 0 47 25 63 846.0 0 63 26 41 846.0 0 41 27 59 846.0 0 59 28 183 846.0 0 183 29 65 846.0 0 65 30 289 846.0 0 289 31 79 846.0 0 79 32 6627 846.0 0 6627 33 81 846.0 0 81 34 58 846.0 0 58 35 68 846.0 0 68 36 33 846.0 0 33 37 61 846.0 0 61 38 43 846.0 0 43 39 53 846.0 0 53 40 45 846.0 0 45 41 63 846.0 0 63 42 54 846.0 0 54 43 37 846.0 0 37 44 54 846.0 0 54 45 48 846.0 0 48 46 58 846.0 0 58 47 41 846.0 0 41 48 53 846.0 0 53 49 49 846.0 0 49 50 53 846.0 0 53 51 62 846.0 0 62 52 50 846.0 0 50 53 46 846.0 0 46 54 51 846.0 0 51 55 64 846.0 0 64 56 79 846.0 0 79 57 49 846.0 0 49 58 51 846.0 0 51 59 42 846.0 0 42 60 56 846.0 0 56 61 58 846.0 0 58 62 161 846.0 0 161 63 154 846.0 0 154 64 206 846.0 0 206 65 87 846.0 0 87 66 131 846.0 0 131 67 153 846.0 0 153 68 4940 846.0 0 4940 69 845 846.0 0 845 70 2666 846.0 0 2666 71 66 846.0 0 66 72 78 846.0 0 78 73 89 846.0 0 89 74 45 846.0 0 45 75 122 846.0 0 122 76 43 846.0 0 43 77 119 846.0 0 119 78 69 846.0 0 69 79 198 846.0 0 198 80 115 846.0 0 115 81 94 846.0 0 94 82 86 846.0 0 86 83 62 846.0 0 62 84 65 846.0 0 65 85 60 846.0 0 60 86 82 846.0 0 82 87 116 846.0 0 116 88 136 846.0 0 136 89 74 846.0 0 74 90 119 846.0 0 119 91 62 846.0 0 62 92 97 846.0 0 97 93 145 846.0 0 145 94 366 846.0 0 366 95 270 846.0 0 270 96 306 846.0 0 306 97 198 846.0 0 198 98 197 846.0 0 197 99 1346 846.0 0 1346 100 2102 846.0 0 2102 101 1517 846.0 0 1517 102 57 846.0 0 57 103 45 846.0 0 45 104 53 846.0 0 53 105 44 846.0 0 44 106 41 846.0 0 41 107 39 846.0 0 39 108 68 846.0 0 68 109 63 846.0 0 63 110 57 846.0 0 57 111 83 846.0 0 83 112 77 846.0 0 77 113 57 846.0 0 57 114 83 846.0 0 83 115 57 846.0 0 57 116 60 846.0 0 60 117 63 846.0 0 63 118 67 846.0 0 67 119 51 846.0 0 51 120 83 846.0 0 83 121 81 846.0 0 81 122 53 846.0 0 53 123 77 846.0 0 77 124 80 846.0 0 80 125 91 846.0 0 91 126 110 846.0 0 110 127 145 846.0 0 145 128 288 846.0 0 288 129 111 846.0 0 111 130 138 846.0 0 138 131 148 846.0 0 148 132 173 846.0 0 173 133 138 846.0 0 138 134 95 846.0 0 95 135 90 846.0 0 90 136 128 846.0 0 128 137 88 846.0 0 88 138 79 846.0 0 79 139 106 846.0 0 106 140 96 846.0 0 96 141 108 846.0 0 108 142 337 846.0 0 337 143 211 846.0 0 211 144 246 846.0 0 246 145 3889 846.0 0 3889 146 402 846.0 0 402 147 389 846.0 0 389 148 77 846.0 0 77 149 98 846.0 0 98 150 64 846.0 0 64 151 54 846.0 0 54 152 65 846.0 0 65 153 88 846.0 0 88 154 51 846.0 0 51 155 62 846.0 0 62 156 80 846.0 0 80 157 66 846.0 0 66 158 74 846.0 0 74 159 83 846.0 0 83 160 58 846.0 0 58 161 69 846.0 0 69 162 68 846.0 0 68 163 82 846.0 0 82 164 82 846.0 0 82 165 80 846.0 0 80 166 78 846.0 0 78 167 175 846.0 0 175 168 113 846.0 0 113 169 83 846.0 0 83 170 52 846.0 0 52 171 51 846.0 0 51 172 50 846.0 0 50 173 82 846.0 0 82 174 87 846.0 0 87 175 109 846.0 0 109 176 177 846.0 0 177 177 82 846.0 0 82 178 73 846.0 0 73 179 64 846.0 0 64 180 71 846.0 0 71 181 72 846.0 0 72 182 61 846.0 0 61 183 75 846.0 0 75 184 77 846.0 0 77 185 86 846.0 0 86 186 73 846.0 0 73 187 67 846.0 0 67 188 67 846.0 0 67 189 70 846.0 0 70 190 87 846.0 0 87 191 184 846.0 0 184 192 189 846.0 0 189 193 416 846.0 0 416 194 368 846.0 0 368 195 161 846.0 0 161 196 113 846.0 0 113 197 183 846.0 0 183 198 460 846.0 0 460 199 554 846.0 0 554 200 612 846.0 0 612 201 194 846.0 0 194 202 101 846.0 0 101 203 158 846.0 0 158 204 128 846.0 0 128 205 137 846.0 0 137 206 155 846.0 0 155 207 169 846.0 0 169 208 110 846.0 0 110 209 105 846.0 0 105 210 106 846.0 0 106 211 213 846.0 0 213 212 169 846.0 0 169 213 149 846.0 0 149 214 114 846.0 0 114 215 94 846.0 0 94 216 160 846.0 0 160 217 77 846.0 0 77 218 85 846.0 0 85 219 198 846.0 0 198 220 126 846.0 0 126 221 162 846.0 0 162 222 118 846.0 0 118 223 124 846.0 0 124 224 107 846.0 0 107 225 77 846.0 0 77 226 89 846.0 0 89 227 77 846.0 0 77 228 81 846.0 0 81 229 67 846.0 0 67 230 83 846.0 0 83 231 120 846.0 0 120 232 240 846.0 0 240 233 222 846.0 0 222 234 238 846.0 0 238 235 120 846.0 0 120 236 105 846.0 0 105 237 80 846.0 0 80 238 97 846.0 0 97 239 102 846.0 0 102 240 118 846.0 0 118 241 152 846.0 0 152 242 150 846.0 0 150 243 159 846.0 0 159 244 107 846.0 0 107 245 73 846.0 0 73 246 69 846.0 0 69 247 78 846.0 0 78 248 64 846.0 0 64 249 64 846.0 0 64 250 111 846.0 0 111 251 66 846.0 0 66 252 123 846.0 0 123 253 142 846.0 0 142 254 108 846.0 0 108 255 125 846.0 0 125 256 95 846.0 0 95 257 63 846.0 0 63 258 61 846.0 0 61 259 52 846.0 0 52 260 70 846.0 0 70 261 103 846.0 0 103 262 66 846.0 0 66 263 87 846.0 0 87 264 76 846.0 0 76 265 95 846.0 0 95 266 66 846.0 0 66 267 89 846.0 0 89 268 105 846.0 0 105 269 80 846.0 0 80 270 57 846.0 0 57 271 51 846.0 0 51 272 78 846.0 0 78 273 85 846.0 0 85 274 59 846.0 0 59 275 50 846.0 0 50 276 44 846.0 0 44 277 58 846.0 0 58 278 63 846.0 0 63 279 84 846.0 0 84 280 63 846.0 0 63 281 38 846.0 0 38 282 56 846.0 0 56 283 96 846.0 0 96 284 91 846.0 0 91 285 61 846.0 0 61 286 50 846.0 0 50 287 38 846.0 0 38 288 58 846.0 0 58 289 42 846.0 0 42 290 46 846.0 0 46 291 59 846.0 0 59 292 106 846.0 0 106 293 68 846.0 0 68 294 44 846.0 0 44 295 43 846.0 0 43 296 38 846.0 0 38 297 51 846.0 0 51 298 39 846.0 0 39 299 60 846.0 0 60 300 90 846.0 0 90 301 64 846.0 0 64 Finished in 3.12 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 68,037 Reads with adapters: 24,338 (35.8%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 24,332 (35.8%) Total basepairs processed: 16,034,366 bp Total written (filtered): 6,639,465 bp (41.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24338 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 13 0.0 3 0 0 11 1 1 19 203 0.0 3 0 177 11 9 6 20 23963 0.0 4 23337 533 69 18 6 21 73 0.0 4 8 60 4 1 22 15 0.0 4 3 1 9 2 23 25 0.0 4 0 0 2 17 6 24 2 0.0 4 1 0 0 0 1 59 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 111 25 0.0 4 0 0 0 21 4 116 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 196 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 24,332 Reads with adapters: 2,817 (11.6%) Reads written (passing filters): 24,332 (100.0%) Total basepairs processed: 6,639,465 bp Total written (filtered): 6,598,459 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2817 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 3.9% G: 93.0% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 380.2 0 1 9 4 0.1 0 0 4 10 122 0.0 1 1 121 11 2634 0.0 1 13 2621 12 10 0.0 1 1 9 151 3 0.0 1 2 1 209 1 0.0 1 1 211 1 0.0 1 1 224 25 0.0 1 24 1 225 3 0.0 1 3 263 2 0.0 1 2 270 4 0.0 1 4 271 5 0.0 1 5 272 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.