This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 90.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 513,429 (14.8%) Reads that were too short: 195,044 (5.6%) Reads written (passing filters): 318,385 (9.2%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 53,658,530 bp (5.1%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 513429 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 140 846.0 0 140 7 234 846.0 0 234 8 288 846.0 0 288 9 22072 846.0 0 22072 10 39 846.0 0 39 11 50 846.0 0 50 12 22 846.0 0 22 13 18 846.0 0 18 14 11 846.0 0 11 15 18 846.0 0 18 16 27 846.0 0 27 17 15 846.0 0 15 18 10 846.0 0 10 19 14 846.0 0 14 20 18 846.0 0 18 21 7 846.0 0 7 22 10 846.0 0 10 23 12 846.0 0 12 24 9 846.0 0 9 25 23 846.0 0 23 26 36 846.0 0 36 27 29 846.0 0 29 28 15 846.0 0 15 29 27 846.0 0 27 30 23 846.0 0 23 31 24 846.0 0 24 32 48 846.0 0 48 33 93 846.0 0 93 34 77 846.0 0 77 35 76 846.0 0 76 36 55 846.0 0 55 37 68 846.0 0 68 38 106 846.0 0 106 39 6273 846.0 0 6273 40 38 846.0 0 38 41 16 846.0 0 16 42 18 846.0 0 18 43 12 846.0 0 12 44 13 846.0 0 13 45 16 846.0 0 16 46 14 846.0 0 14 47 23 846.0 0 23 48 10 846.0 0 10 49 22 846.0 0 22 50 15 846.0 0 15 51 14 846.0 0 14 52 7 846.0 0 7 53 16 846.0 0 16 54 24 846.0 0 24 55 19 846.0 0 19 56 16 846.0 0 16 57 11 846.0 0 11 58 11 846.0 0 11 59 77 846.0 0 77 60 19 846.0 0 19 61 14 846.0 0 14 62 36 846.0 0 36 63 707 846.0 0 707 64 36 846.0 0 36 65 24 846.0 0 24 66 22 846.0 0 22 67 20 846.0 0 20 68 230 846.0 0 230 69 30 846.0 0 30 70 19 846.0 0 19 71 20 846.0 0 20 72 47 846.0 0 47 73 32 846.0 0 32 74 68 846.0 0 68 75 59 846.0 0 59 76 26 846.0 0 26 77 24 846.0 0 24 78 119 846.0 0 119 79 764 846.0 0 764 80 2225 846.0 0 2225 81 2047 846.0 0 2047 82 2691 846.0 0 2691 83 2717 846.0 0 2717 84 2443 846.0 0 2443 85 3267 846.0 0 3267 86 3317 846.0 0 3317 87 2222 846.0 0 2222 88 1550 846.0 0 1550 89 1185 846.0 0 1185 90 556 846.0 0 556 91 1236 846.0 0 1236 92 4710 846.0 0 4710 93 1604 846.0 0 1604 94 2184 846.0 0 2184 95 2202 846.0 0 2202 96 1100 846.0 0 1100 97 782 846.0 0 782 98 1013 846.0 0 1013 99 1033 846.0 0 1033 100 2759 846.0 0 2759 101 2519 846.0 0 2519 102 1798 846.0 0 1798 103 2373 846.0 0 2373 104 1359 846.0 0 1359 105 728 846.0 0 728 106 656 846.0 0 656 107 557 846.0 0 557 108 1430 846.0 0 1430 109 2164 846.0 0 2164 110 2418 846.0 0 2418 111 1825 846.0 0 1825 112 2032 846.0 0 2032 113 1496 846.0 0 1496 114 1402 846.0 0 1402 115 1596 846.0 0 1596 116 2429 846.0 0 2429 117 2899 846.0 0 2899 118 3030 846.0 0 3030 119 2827 846.0 0 2827 120 3057 846.0 0 3057 121 2587 846.0 0 2587 122 2807 846.0 0 2807 123 2071 846.0 0 2071 124 1937 846.0 0 1937 125 2180 846.0 0 2180 126 1769 846.0 0 1769 127 1433 846.0 0 1433 128 1689 846.0 0 1689 129 2162 846.0 0 2162 130 1865 846.0 0 1865 131 3051 846.0 0 3051 132 2150 846.0 0 2150 133 1729 846.0 0 1729 134 1682 846.0 0 1682 135 1749 846.0 0 1749 136 1923 846.0 0 1923 137 2336 846.0 0 2336 138 2648 846.0 0 2648 139 2473 846.0 0 2473 140 1906 846.0 0 1906 141 1940 846.0 0 1940 142 2530 846.0 0 2530 143 2146 846.0 0 2146 144 2092 846.0 0 2092 145 2754 846.0 0 2754 146 2548 846.0 0 2548 147 2508 846.0 0 2508 148 2268 846.0 0 2268 149 1975 846.0 0 1975 150 2238 846.0 0 2238 151 2126 846.0 0 2126 152 2576 846.0 0 2576 153 2645 846.0 0 2645 154 2020 846.0 0 2020 155 2435 846.0 0 2435 156 1924 846.0 0 1924 157 2103 846.0 0 2103 158 2353 846.0 0 2353 159 2184 846.0 0 2184 160 2349 846.0 0 2349 161 1881 846.0 0 1881 162 2169 846.0 0 2169 163 1734 846.0 0 1734 164 1916 846.0 0 1916 165 1500 846.0 0 1500 166 3685 846.0 0 3685 167 5656 846.0 0 5656 168 3033 846.0 0 3033 169 2360 846.0 0 2360 170 2702 846.0 0 2702 171 3277 846.0 0 3277 172 2898 846.0 0 2898 173 1861 846.0 0 1861 174 1808 846.0 0 1808 175 2315 846.0 0 2315 176 2149 846.0 0 2149 177 2309 846.0 0 2309 178 2379 846.0 0 2379 179 2114 846.0 0 2114 180 2378 846.0 0 2378 181 2616 846.0 0 2616 182 7982 846.0 0 7982 183 16876 846.0 0 16876 184 3060 846.0 0 3060 185 2556 846.0 0 2556 186 1804 846.0 0 1804 187 2207 846.0 0 2207 188 2234 846.0 0 2234 189 2041 846.0 0 2041 190 1894 846.0 0 1894 191 1947 846.0 0 1947 192 1856 846.0 0 1856 193 1776 846.0 0 1776 194 1741 846.0 0 1741 195 1846 846.0 0 1846 196 1661 846.0 0 1661 197 2433 846.0 0 2433 198 2933 846.0 0 2933 199 2289 846.0 0 2289 200 2893 846.0 0 2893 201 1622 846.0 0 1622 202 1741 846.0 0 1741 203 2117 846.0 0 2117 204 2145 846.0 0 2145 205 2109 846.0 0 2109 206 2031 846.0 0 2031 207 1884 846.0 0 1884 208 1633 846.0 0 1633 209 1544 846.0 0 1544 210 1709 846.0 0 1709 211 1827 846.0 0 1827 212 1920 846.0 0 1920 213 2896 846.0 0 2896 214 2813 846.0 0 2813 215 2861 846.0 0 2861 216 1870 846.0 0 1870 217 1781 846.0 0 1781 218 2248 846.0 0 2248 219 2568 846.0 0 2568 220 1812 846.0 0 1812 221 1506 846.0 0 1506 222 1581 846.0 0 1581 223 2040 846.0 0 2040 224 1906 846.0 0 1906 225 1462 846.0 0 1462 226 1504 846.0 0 1504 227 1531 846.0 0 1531 228 1962 846.0 0 1962 229 1662 846.0 0 1662 230 1544 846.0 0 1544 231 1334 846.0 0 1334 232 1664 846.0 0 1664 233 1724 846.0 0 1724 234 1588 846.0 0 1588 235 1570 846.0 0 1570 236 1319 846.0 0 1319 237 1508 846.0 0 1508 238 1791 846.0 0 1791 239 2071 846.0 0 2071 240 1692 846.0 0 1692 241 1496 846.0 0 1496 242 1788 846.0 0 1788 243 1605 846.0 0 1605 244 1538 846.0 0 1538 245 1469 846.0 0 1469 246 1824 846.0 0 1824 247 1626 846.0 0 1626 248 1822 846.0 0 1822 249 1444 846.0 0 1444 250 1866 846.0 0 1866 251 1743 846.0 0 1743 252 2560 846.0 0 2560 253 1856 846.0 0 1856 254 1626 846.0 0 1626 255 1423 846.0 0 1423 256 1482 846.0 0 1482 257 1286 846.0 0 1286 258 1425 846.0 0 1425 259 1207 846.0 0 1207 260 1305 846.0 0 1305 261 1463 846.0 0 1463 262 2309 846.0 0 2309 263 2240 846.0 0 2240 264 1824 846.0 0 1824 265 1497 846.0 0 1497 266 1830 846.0 0 1830 267 12007 846.0 0 12007 268 6115 846.0 0 6115 269 5779 846.0 0 5779 270 1970 846.0 0 1970 271 1520 846.0 0 1520 272 2097 846.0 0 2097 273 1956 846.0 0 1956 274 1404 846.0 0 1404 275 1157 846.0 0 1157 276 1294 846.0 0 1294 277 1401 846.0 0 1401 278 1618 846.0 0 1618 279 1594 846.0 0 1594 280 1230 846.0 0 1230 281 1585 846.0 0 1585 282 1280 846.0 0 1280 283 1494 846.0 0 1494 284 1430 846.0 0 1430 285 1077 846.0 0 1077 286 1400 846.0 0 1400 287 1339 846.0 0 1339 288 1233 846.0 0 1233 289 1153 846.0 0 1153 290 1631 846.0 0 1631 291 2039 846.0 0 2039 292 2546 846.0 0 2546 293 1650 846.0 0 1650 294 1381 846.0 0 1381 295 1316 846.0 0 1316 296 1562 846.0 0 1562 297 2230 846.0 0 2230 298 2558 846.0 0 2558 299 3152 846.0 0 3152 300 2715 846.0 0 2715 301 3109 846.0 0 3109 Finished in 9.84 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 318,385 Reads with adapters: 22,641 (7.1%) Reads that were too short: 63 (0.0%) Reads written (passing filters): 22,578 (7.1%) Total basepairs processed: 53,658,530 bp Total written (filtered): 6,198,565 bp (11.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22641 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 6 1 16 151 0.0 3 0 145 5 1 17 22256 0.0 3 21832 412 11 1 18 48 0.0 3 4 42 1 1 19 21 0.0 3 2 0 12 7 20 20 0.0 3 0 0 0 20 21 9 0.0 3 0 0 0 9 24 9 0.0 3 0 0 0 9 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 30 3 0.0 3 1 1 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 3 0.0 3 1 0 0 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 1 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 62 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 1 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 6 0.0 3 0 0 0 6 73 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 0 4 106 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 3 0.0 3 0 0 0 3 109 2 0.0 3 0 0 0 2 111 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 136 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.82 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 22,578 Reads with adapters: 1,951 (8.6%) Reads written (passing filters): 22,578 (100.0%) Total basepairs processed: 6,198,565 bp Total written (filtered): 6,192,708 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1951 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.0% C: 4.7% G: 62.6% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1949 352.8 0 1949 4 1 88.2 0 1 6 1 5.5 0 1