This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.69 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 126,051 (3.6%) Reads that were too short: 62,821 (1.8%) Reads written (passing filters): 63,230 (1.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 12,852,792 bp (1.2%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 126051 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 214 846.0 0 214 7 246 846.0 0 246 8 889 846.0 0 889 9 18337 846.0 0 18337 10 95 846.0 0 95 11 39 846.0 0 39 12 15 846.0 0 15 13 76 846.0 0 76 14 16 846.0 0 16 15 11 846.0 0 11 16 13 846.0 0 13 17 40 846.0 0 40 18 11 846.0 0 11 19 10 846.0 0 10 20 16 846.0 0 16 21 14 846.0 0 14 22 11 846.0 0 11 23 13 846.0 0 13 24 21 846.0 0 21 25 21 846.0 0 21 26 16 846.0 0 16 27 17 846.0 0 17 28 16 846.0 0 16 29 18 846.0 0 18 30 13 846.0 0 13 31 13 846.0 0 13 32 18 846.0 0 18 33 16 846.0 0 16 34 12 846.0 0 12 35 16 846.0 0 16 36 12 846.0 0 12 37 10 846.0 0 10 38 25 846.0 0 25 39 14 846.0 0 14 40 15 846.0 0 15 41 15 846.0 0 15 42 16 846.0 0 16 43 15 846.0 0 15 44 18 846.0 0 18 45 13 846.0 0 13 46 9 846.0 0 9 47 7 846.0 0 7 48 9 846.0 0 9 49 13 846.0 0 13 50 16 846.0 0 16 51 14 846.0 0 14 52 25 846.0 0 25 53 16 846.0 0 16 54 12 846.0 0 12 55 10 846.0 0 10 56 20 846.0 0 20 57 15 846.0 0 15 58 12 846.0 0 12 59 15 846.0 0 15 60 17 846.0 0 17 61 15 846.0 0 15 62 47 846.0 0 47 63 15 846.0 0 15 64 16 846.0 0 16 65 22 846.0 0 22 66 19 846.0 0 19 67 24 846.0 0 24 68 23 846.0 0 23 69 16 846.0 0 16 70 130 846.0 0 130 71 499 846.0 0 499 72 920 846.0 0 920 73 166 846.0 0 166 74 142 846.0 0 142 75 93 846.0 0 93 76 399 846.0 0 399 77 298 846.0 0 298 78 211 846.0 0 211 79 127 846.0 0 127 80 129 846.0 0 129 81 211 846.0 0 211 82 385 846.0 0 385 83 746 846.0 0 746 84 530 846.0 0 530 85 450 846.0 0 450 86 223 846.0 0 223 87 127 846.0 0 127 88 71 846.0 0 71 89 162 846.0 0 162 90 198 846.0 0 198 91 141 846.0 0 141 92 597 846.0 0 597 93 529 846.0 0 529 94 225 846.0 0 225 95 323 846.0 0 323 96 159 846.0 0 159 97 118 846.0 0 118 98 137 846.0 0 137 99 82 846.0 0 82 100 160 846.0 0 160 101 212 846.0 0 212 102 120 846.0 0 120 103 332 846.0 0 332 104 158 846.0 0 158 105 62 846.0 0 62 106 85 846.0 0 85 107 261 846.0 0 261 108 262 846.0 0 262 109 181 846.0 0 181 110 199 846.0 0 199 111 198 846.0 0 198 112 204 846.0 0 204 113 223 846.0 0 223 114 264 846.0 0 264 115 216 846.0 0 216 116 286 846.0 0 286 117 317 846.0 0 317 118 227 846.0 0 227 119 312 846.0 0 312 120 307 846.0 0 307 121 259 846.0 0 259 122 228 846.0 0 228 123 347 846.0 0 347 124 400 846.0 0 400 125 440 846.0 0 440 126 885 846.0 0 885 127 789 846.0 0 789 128 335 846.0 0 335 129 243 846.0 0 243 130 174 846.0 0 174 131 208 846.0 0 208 132 251 846.0 0 251 133 283 846.0 0 283 134 389 846.0 0 389 135 303 846.0 0 303 136 380 846.0 0 380 137 363 846.0 0 363 138 275 846.0 0 275 139 312 846.0 0 312 140 371 846.0 0 371 141 276 846.0 0 276 142 190 846.0 0 190 143 236 846.0 0 236 144 357 846.0 0 357 145 351 846.0 0 351 146 301 846.0 0 301 147 264 846.0 0 264 148 316 846.0 0 316 149 215 846.0 0 215 150 322 846.0 0 322 151 248 846.0 0 248 152 322 846.0 0 322 153 492 846.0 0 492 154 376 846.0 0 376 155 384 846.0 0 384 156 392 846.0 0 392 157 734 846.0 0 734 158 383 846.0 0 383 159 271 846.0 0 271 160 369 846.0 0 369 161 210 846.0 0 210 162 208 846.0 0 208 163 251 846.0 0 251 164 384 846.0 0 384 165 396 846.0 0 396 166 317 846.0 0 317 167 243 846.0 0 243 168 262 846.0 0 262 169 370 846.0 0 370 170 444 846.0 0 444 171 358 846.0 0 358 172 462 846.0 0 462 173 692 846.0 0 692 174 449 846.0 0 449 175 485 846.0 0 485 176 308 846.0 0 308 177 320 846.0 0 320 178 358 846.0 0 358 179 348 846.0 0 348 180 434 846.0 0 434 181 461 846.0 0 461 182 318 846.0 0 318 183 295 846.0 0 295 184 243 846.0 0 243 185 372 846.0 0 372 186 304 846.0 0 304 187 220 846.0 0 220 188 188 846.0 0 188 189 427 846.0 0 427 190 743 846.0 0 743 191 518 846.0 0 518 192 410 846.0 0 410 193 375 846.0 0 375 194 170 846.0 0 170 195 326 846.0 0 326 196 366 846.0 0 366 197 903 846.0 0 903 198 692 846.0 0 692 199 200 846.0 0 200 200 189 846.0 0 189 201 240 846.0 0 240 202 556 846.0 0 556 203 620 846.0 0 620 204 331 846.0 0 331 205 363 846.0 0 363 206 310 846.0 0 310 207 298 846.0 0 298 208 215 846.0 0 215 209 195 846.0 0 195 210 194 846.0 0 194 211 243 846.0 0 243 212 212 846.0 0 212 213 271 846.0 0 271 214 303 846.0 0 303 215 301 846.0 0 301 216 540 846.0 0 540 217 1865 846.0 0 1865 218 675 846.0 0 675 219 470 846.0 0 470 220 267 846.0 0 267 221 241 846.0 0 241 222 451 846.0 0 451 223 458 846.0 0 458 224 326 846.0 0 326 225 474 846.0 0 474 226 497 846.0 0 497 227 556 846.0 0 556 228 791 846.0 0 791 229 3005 846.0 0 3005 230 3435 846.0 0 3435 231 964 846.0 0 964 232 253 846.0 0 253 233 306 846.0 0 306 234 335 846.0 0 335 235 389 846.0 0 389 236 269 846.0 0 269 237 193 846.0 0 193 238 209 846.0 0 209 239 263 846.0 0 263 240 147 846.0 0 147 241 148 846.0 0 148 242 170 846.0 0 170 243 230 846.0 0 230 244 266 846.0 0 266 245 520 846.0 0 520 246 427 846.0 0 427 247 329 846.0 0 329 248 162 846.0 0 162 249 85 846.0 0 85 250 180 846.0 0 180 251 349 846.0 0 349 252 545 846.0 0 545 253 365 846.0 0 365 254 302 846.0 0 302 255 249 846.0 0 249 256 300 846.0 0 300 257 249 846.0 0 249 258 220 846.0 0 220 259 321 846.0 0 321 260 1161 846.0 0 1161 261 879 846.0 0 879 262 615 846.0 0 615 263 431 846.0 0 431 264 513 846.0 0 513 265 490 846.0 0 490 266 737 846.0 0 737 267 471 846.0 0 471 268 400 846.0 0 400 269 176 846.0 0 176 270 369 846.0 0 369 271 381 846.0 0 381 272 309 846.0 0 309 273 434 846.0 0 434 274 233 846.0 0 233 275 248 846.0 0 248 276 494 846.0 0 494 277 444 846.0 0 444 278 599 846.0 0 599 279 656 846.0 0 656 280 400 846.0 0 400 281 161 846.0 0 161 282 255 846.0 0 255 283 487 846.0 0 487 284 698 846.0 0 698 285 566 846.0 0 566 286 479 846.0 0 479 287 1005 846.0 0 1005 288 891 846.0 0 891 289 557 846.0 0 557 290 808 846.0 0 808 291 645 846.0 0 645 292 762 846.0 0 762 293 4967 846.0 0 4967 294 4612 846.0 0 4612 295 6484 846.0 0 6484 296 259 846.0 0 259 297 158 846.0 0 158 298 238 846.0 0 238 299 261 846.0 0 261 300 257 846.0 0 257 301 623 846.0 0 623 Finished in 2.65 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 63,230 Reads with adapters: 19,632 (31.0%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 19,605 (31.0%) Total basepairs processed: 12,852,792 bp Total written (filtered): 5,331,114 bp (41.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19632 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 6 0.0 3 0 0 5 0 1 19 156 0.0 3 0 147 7 2 20 19334 0.0 4 18305 903 84 31 11 21 53 0.0 4 9 38 2 1 3 22 7 0.0 4 1 0 2 0 4 23 16 0.0 4 0 0 1 11 4 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 1 32 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 46 8 0.0 4 0 0 0 6 2 47 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 1 1 108 2 0.0 4 0 0 0 2 110 5 0.0 4 0 0 0 1 4 111 15 0.0 4 0 0 0 13 2 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.67 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 19,605 Reads with adapters: 1,273 (6.5%) Reads written (passing filters): 19,605 (100.0%) Total basepairs processed: 5,331,114 bp Total written (filtered): 5,306,616 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1273 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.5% C: 6.4% G: 87.7% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 3 76.6 0 3 9 165 0.1 0 14 151 10 614 0.0 1 34 580 11 438 0.0 1 1 437 13 1 0.0 1 0 1 150 2 0.0 1 1 1 208 2 0.0 1 2 223 32 0.0 1 31 1 224 1 0.0 1 1 225 1 0.0 1 1 256 1 0.0 1 1 261 1 0.0 1 1 262 2 0.0 1 2 263 2 0.0 1 2 269 2 0.0 1 2 270 3 0.0 1 3 271 2 0.0 1 2 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.