This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.44 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 139,570 (4.0%) Reads that were too short: 59,153 (1.7%) Reads written (passing filters): 80,417 (2.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 14,856,332 bp (1.4%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 139570 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 157 846.0 0 157 7 19712 846.0 0 19712 8 63 846.0 0 63 9 9 846.0 0 9 10 8 846.0 0 8 11 6 846.0 0 6 12 8 846.0 0 8 13 71 846.0 0 71 14 103 846.0 0 103 15 78 846.0 0 78 16 20 846.0 0 20 17 5 846.0 0 5 18 7 846.0 0 7 19 6 846.0 0 6 20 5 846.0 0 5 21 6 846.0 0 6 22 6 846.0 0 6 23 14 846.0 0 14 24 20 846.0 0 20 25 998 846.0 0 998 26 86 846.0 0 86 27 9 846.0 0 9 28 11 846.0 0 11 29 2 846.0 0 2 30 13 846.0 0 13 31 12 846.0 0 12 32 6 846.0 0 6 33 5 846.0 0 5 34 8 846.0 0 8 35 5 846.0 0 5 36 11 846.0 0 11 37 7 846.0 0 7 38 9 846.0 0 9 39 11 846.0 0 11 40 6 846.0 0 6 41 2 846.0 0 2 42 9 846.0 0 9 43 9 846.0 0 9 44 9 846.0 0 9 45 7 846.0 0 7 46 8 846.0 0 8 47 8 846.0 0 8 48 9 846.0 0 9 49 6 846.0 0 6 50 14 846.0 0 14 51 12 846.0 0 12 52 6 846.0 0 6 53 7 846.0 0 7 54 7 846.0 0 7 55 6 846.0 0 6 56 19 846.0 0 19 57 10 846.0 0 10 58 12 846.0 0 12 59 11 846.0 0 11 60 27 846.0 0 27 61 37 846.0 0 37 62 120 846.0 0 120 63 16 846.0 0 16 64 5 846.0 0 5 65 16 846.0 0 16 66 11 846.0 0 11 67 14 846.0 0 14 68 8 846.0 0 8 69 16 846.0 0 16 70 11 846.0 0 11 71 11 846.0 0 11 72 11 846.0 0 11 73 12 846.0 0 12 74 9 846.0 0 9 75 17 846.0 0 17 76 15 846.0 0 15 77 15 846.0 0 15 78 11 846.0 0 11 79 19 846.0 0 19 80 48 846.0 0 48 81 145 846.0 0 145 82 426 846.0 0 426 83 365 846.0 0 365 84 349 846.0 0 349 85 111 846.0 0 111 86 76 846.0 0 76 87 130 846.0 0 130 88 616 846.0 0 616 89 303 846.0 0 303 90 234 846.0 0 234 91 157 846.0 0 157 92 76 846.0 0 76 93 142 846.0 0 142 94 289 846.0 0 289 95 194 846.0 0 194 96 318 846.0 0 318 97 158 846.0 0 158 98 233 846.0 0 233 99 338 846.0 0 338 100 226 846.0 0 226 101 204 846.0 0 204 102 366 846.0 0 366 103 515 846.0 0 515 104 472 846.0 0 472 105 215 846.0 0 215 106 214 846.0 0 214 107 261 846.0 0 261 108 90 846.0 0 90 109 64 846.0 0 64 110 157 846.0 0 157 111 402 846.0 0 402 112 508 846.0 0 508 113 291 846.0 0 291 114 317 846.0 0 317 115 186 846.0 0 186 116 171 846.0 0 171 117 125 846.0 0 125 118 343 846.0 0 343 119 433 846.0 0 433 120 561 846.0 0 561 121 275 846.0 0 275 122 287 846.0 0 287 123 428 846.0 0 428 124 422 846.0 0 422 125 247 846.0 0 247 126 240 846.0 0 240 127 333 846.0 0 333 128 216 846.0 0 216 129 290 846.0 0 290 130 324 846.0 0 324 131 266 846.0 0 266 132 312 846.0 0 312 133 362 846.0 0 362 134 494 846.0 0 494 135 308 846.0 0 308 136 264 846.0 0 264 137 278 846.0 0 278 138 365 846.0 0 365 139 682 846.0 0 682 140 846 846.0 0 846 141 591 846.0 0 591 142 306 846.0 0 306 143 311 846.0 0 311 144 420 846.0 0 420 145 528 846.0 0 528 146 375 846.0 0 375 147 401 846.0 0 401 148 379 846.0 0 379 149 275 846.0 0 275 150 238 846.0 0 238 151 317 846.0 0 317 152 404 846.0 0 404 153 461 846.0 0 461 154 354 846.0 0 354 155 385 846.0 0 385 156 334 846.0 0 334 157 392 846.0 0 392 158 465 846.0 0 465 159 532 846.0 0 532 160 1373 846.0 0 1373 161 1681 846.0 0 1681 162 788 846.0 0 788 163 343 846.0 0 343 164 377 846.0 0 377 165 359 846.0 0 359 166 255 846.0 0 255 167 293 846.0 0 293 168 480 846.0 0 480 169 588 846.0 0 588 170 348 846.0 0 348 171 293 846.0 0 293 172 363 846.0 0 363 173 504 846.0 0 504 174 360 846.0 0 360 175 370 846.0 0 370 176 381 846.0 0 381 177 465 846.0 0 465 178 394 846.0 0 394 179 456 846.0 0 456 180 582 846.0 0 582 181 473 846.0 0 473 182 630 846.0 0 630 183 473 846.0 0 473 184 451 846.0 0 451 185 584 846.0 0 584 186 691 846.0 0 691 187 9675 846.0 0 9675 188 792 846.0 0 792 189 2982 846.0 0 2982 190 506 846.0 0 506 191 374 846.0 0 374 192 309 846.0 0 309 193 362 846.0 0 362 194 257 846.0 0 257 195 355 846.0 0 355 196 500 846.0 0 500 197 530 846.0 0 530 198 352 846.0 0 352 199 314 846.0 0 314 200 390 846.0 0 390 201 753 846.0 0 753 202 1033 846.0 0 1033 203 413 846.0 0 413 204 405 846.0 0 405 205 563 846.0 0 563 206 599 846.0 0 599 207 678 846.0 0 678 208 581 846.0 0 581 209 500 846.0 0 500 210 610 846.0 0 610 211 487 846.0 0 487 212 503 846.0 0 503 213 436 846.0 0 436 214 528 846.0 0 528 215 434 846.0 0 434 216 410 846.0 0 410 217 421 846.0 0 421 218 469 846.0 0 469 219 548 846.0 0 548 220 621 846.0 0 621 221 678 846.0 0 678 222 623 846.0 0 623 223 450 846.0 0 450 224 401 846.0 0 401 225 480 846.0 0 480 226 440 846.0 0 440 227 522 846.0 0 522 228 396 846.0 0 396 229 570 846.0 0 570 230 988 846.0 0 988 231 700 846.0 0 700 232 590 846.0 0 590 233 557 846.0 0 557 234 586 846.0 0 586 235 431 846.0 0 431 236 314 846.0 0 314 237 369 846.0 0 369 238 538 846.0 0 538 239 643 846.0 0 643 240 772 846.0 0 772 241 819 846.0 0 819 242 748 846.0 0 748 243 499 846.0 0 499 244 404 846.0 0 404 245 546 846.0 0 546 246 477 846.0 0 477 247 295 846.0 0 295 248 227 846.0 0 227 249 213 846.0 0 213 250 589 846.0 0 589 251 827 846.0 0 827 252 511 846.0 0 511 253 377 846.0 0 377 254 405 846.0 0 405 255 413 846.0 0 413 256 513 846.0 0 513 257 487 846.0 0 487 258 489 846.0 0 489 259 342 846.0 0 342 260 408 846.0 0 408 261 436 846.0 0 436 262 475 846.0 0 475 263 457 846.0 0 457 264 591 846.0 0 591 265 449 846.0 0 449 266 608 846.0 0 608 267 405 846.0 0 405 268 299 846.0 0 299 269 388 846.0 0 388 270 775 846.0 0 775 271 620 846.0 0 620 272 401 846.0 0 401 273 392 846.0 0 392 274 612 846.0 0 612 275 1850 846.0 0 1850 276 557 846.0 0 557 277 804 846.0 0 804 278 871 846.0 0 871 279 766 846.0 0 766 280 384 846.0 0 384 281 468 846.0 0 468 282 736 846.0 0 736 283 912 846.0 0 912 284 548 846.0 0 548 285 589 846.0 0 589 286 536 846.0 0 536 287 719 846.0 0 719 288 350 846.0 0 350 289 525 846.0 0 525 290 678 846.0 0 678 291 578 846.0 0 578 292 581 846.0 0 581 293 490 846.0 0 490 294 453 846.0 0 453 295 608 846.0 0 608 296 763 846.0 0 763 297 612 846.0 0 612 298 590 846.0 0 590 299 671 846.0 0 671 300 1523 846.0 0 1523 301 3207 846.0 0 3207 Finished in 2.93 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 80,417 Reads with adapters: 19,842 (24.7%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 19,800 (24.6%) Total basepairs processed: 14,856,332 bp Total written (filtered): 5,482,055 bp (36.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19842 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 5 0.0 3 0 0 4 1 16 155 0.0 3 0 154 1 17 19549 0.0 3 19348 195 5 1 18 52 0.0 3 3 46 2 1 19 4 0.0 3 0 0 4 20 18 0.0 3 0 0 0 18 33 3 0.0 3 0 0 0 3 37 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 141 27 0.0 3 0 0 0 27 148 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.70 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 19,800 Reads with adapters: 948 (4.8%) Reads written (passing filters): 19,800 (100.0%) Total basepairs processed: 5,482,055 bp Total written (filtered): 5,479,210 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 948 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.2% C: 94.1% G: 2.4% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 947 309.4 0 947 4 1 77.3 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.