This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.48 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 820,785 (23.7%) Reads that were too short: 442,781 (12.8%) Reads written (passing filters): 378,004 (10.9%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 63,647,227 bp (6.1%) === Adapter 1 === Sequence: AACAAC; Type: regular 5'; Length: 6; Trimmed: 820785 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 237 846.0 0 237 7 83 846.0 0 83 8 1038 846.0 0 1038 9 43 846.0 0 43 10 47 846.0 0 47 11 46 846.0 0 46 12 35 846.0 0 35 13 36 846.0 0 36 14 45 846.0 0 45 15 39 846.0 0 39 16 37 846.0 0 37 17 37 846.0 0 37 18 36 846.0 0 36 19 34 846.0 0 34 20 26 846.0 0 26 21 34 846.0 0 34 22 41 846.0 0 41 23 24 846.0 0 24 24 42 846.0 0 42 25 28 846.0 0 28 26 57 846.0 0 57 27 53 846.0 0 53 28 32 846.0 0 32 29 37 846.0 0 37 30 35 846.0 0 35 31 35 846.0 0 35 32 41 846.0 0 41 33 23 846.0 0 23 34 30 846.0 0 30 35 24 846.0 0 24 36 43 846.0 0 43 37 29 846.0 0 29 38 34 846.0 0 34 39 47 846.0 0 47 40 36 846.0 0 36 41 35 846.0 0 35 42 27 846.0 0 27 43 59 846.0 0 59 44 38 846.0 0 38 45 40 846.0 0 40 46 37 846.0 0 37 47 41 846.0 0 41 48 41 846.0 0 41 49 29 846.0 0 29 50 39 846.0 0 39 51 41 846.0 0 41 52 30 846.0 0 30 53 45 846.0 0 45 54 42 846.0 0 42 55 56 846.0 0 56 56 57 846.0 0 57 57 35 846.0 0 35 58 34 846.0 0 34 59 40 846.0 0 40 60 35 846.0 0 35 61 51 846.0 0 51 62 121 846.0 0 121 63 197 846.0 0 197 64 199 846.0 0 199 65 263 846.0 0 263 66 199 846.0 0 199 67 784 846.0 0 784 68 341 846.0 0 341 69 332 846.0 0 332 70 2289 846.0 0 2289 71 8640 846.0 0 8640 72 5606 846.0 0 5606 73 3610 846.0 0 3610 74 4536 846.0 0 4536 75 1933 846.0 0 1933 76 1588 846.0 0 1588 77 2223 846.0 0 2223 78 3078 846.0 0 3078 79 1971 846.0 0 1971 80 1996 846.0 0 1996 81 3274 846.0 0 3274 82 2229 846.0 0 2229 83 3530 846.0 0 3530 84 2680 846.0 0 2680 85 1797 846.0 0 1797 86 1826 846.0 0 1826 87 2614 846.0 0 2614 88 1457 846.0 0 1457 89 2308 846.0 0 2308 90 3947 846.0 0 3947 91 2326 846.0 0 2326 92 2551 846.0 0 2551 93 2039 846.0 0 2039 94 1417 846.0 0 1417 95 1214 846.0 0 1214 96 815 846.0 0 815 97 1109 846.0 0 1109 98 1333 846.0 0 1333 99 973 846.0 0 973 100 1983 846.0 0 1983 101 2912 846.0 0 2912 102 2137 846.0 0 2137 103 3501 846.0 0 3501 104 5529 846.0 0 5529 105 5876 846.0 0 5876 106 2926 846.0 0 2926 107 3864 846.0 0 3864 108 4046 846.0 0 4046 109 3316 846.0 0 3316 110 4023 846.0 0 4023 111 3343 846.0 0 3343 112 3178 846.0 0 3178 113 2678 846.0 0 2678 114 2838 846.0 0 2838 115 2986 846.0 0 2986 116 2507 846.0 0 2507 117 2616 846.0 0 2616 118 2728 846.0 0 2728 119 2714 846.0 0 2714 120 3296 846.0 0 3296 121 3518 846.0 0 3518 122 2898 846.0 0 2898 123 2907 846.0 0 2907 124 3587 846.0 0 3587 125 4334 846.0 0 4334 126 4047 846.0 0 4047 127 3091 846.0 0 3091 128 2718 846.0 0 2718 129 2643 846.0 0 2643 130 2575 846.0 0 2575 131 2710 846.0 0 2710 132 3040 846.0 0 3040 133 2708 846.0 0 2708 134 2903 846.0 0 2903 135 2362 846.0 0 2362 136 2489 846.0 0 2489 137 2632 846.0 0 2632 138 2280 846.0 0 2280 139 2240 846.0 0 2240 140 2706 846.0 0 2706 141 2868 846.0 0 2868 142 2976 846.0 0 2976 143 2785 846.0 0 2785 144 2781 846.0 0 2781 145 3158 846.0 0 3158 146 2997 846.0 0 2997 147 3099 846.0 0 3099 148 2753 846.0 0 2753 149 3432 846.0 0 3432 150 3422 846.0 0 3422 151 2880 846.0 0 2880 152 3241 846.0 0 3241 153 3182 846.0 0 3182 154 2985 846.0 0 2985 155 2420 846.0 0 2420 156 2522 846.0 0 2522 157 2546 846.0 0 2546 158 2520 846.0 0 2520 159 2656 846.0 0 2656 160 2790 846.0 0 2790 161 3137 846.0 0 3137 162 3183 846.0 0 3183 163 3232 846.0 0 3232 164 2371 846.0 0 2371 165 2313 846.0 0 2313 166 2599 846.0 0 2599 167 2466 846.0 0 2466 168 2680 846.0 0 2680 169 3075 846.0 0 3075 170 3699 846.0 0 3699 171 3900 846.0 0 3900 172 3362 846.0 0 3362 173 2739 846.0 0 2739 174 3201 846.0 0 3201 175 2781 846.0 0 2781 176 2199 846.0 0 2199 177 2855 846.0 0 2855 178 3071 846.0 0 3071 179 2799 846.0 0 2799 180 2674 846.0 0 2674 181 2882 846.0 0 2882 182 2930 846.0 0 2930 183 2867 846.0 0 2867 184 2179 846.0 0 2179 185 1886 846.0 0 1886 186 2244 846.0 0 2244 187 2727 846.0 0 2727 188 2491 846.0 0 2491 189 2498 846.0 0 2498 190 2872 846.0 0 2872 191 2626 846.0 0 2626 192 2369 846.0 0 2369 193 1843 846.0 0 1843 194 2689 846.0 0 2689 195 2622 846.0 0 2622 196 1741 846.0 0 1741 197 2344 846.0 0 2344 198 2589 846.0 0 2589 199 2691 846.0 0 2691 200 2553 846.0 0 2553 201 1886 846.0 0 1886 202 2187 846.0 0 2187 203 2683 846.0 0 2683 204 2522 846.0 0 2522 205 1900 846.0 0 1900 206 1660 846.0 0 1660 207 1760 846.0 0 1760 208 1988 846.0 0 1988 209 1773 846.0 0 1773 210 1646 846.0 0 1646 211 1893 846.0 0 1893 212 2125 846.0 0 2125 213 2616 846.0 0 2616 214 2728 846.0 0 2728 215 2470 846.0 0 2470 216 2247 846.0 0 2247 217 2318 846.0 0 2318 218 3025 846.0 0 3025 219 2116 846.0 0 2116 220 2772 846.0 0 2772 221 2083 846.0 0 2083 222 1980 846.0 0 1980 223 2429 846.0 0 2429 224 2652 846.0 0 2652 225 3054 846.0 0 3054 226 4619 846.0 0 4619 227 3563 846.0 0 3563 228 3677 846.0 0 3677 229 3148 846.0 0 3148 230 5489 846.0 0 5489 231 4040 846.0 0 4040 232 3223 846.0 0 3223 233 2274 846.0 0 2274 234 1998 846.0 0 1998 235 2027 846.0 0 2027 236 2318 846.0 0 2318 237 2008 846.0 0 2008 238 1776 846.0 0 1776 239 3054 846.0 0 3054 240 2755 846.0 0 2755 241 2158 846.0 0 2158 242 1954 846.0 0 1954 243 2486 846.0 0 2486 244 2078 846.0 0 2078 245 1568 846.0 0 1568 246 1903 846.0 0 1903 247 2390 846.0 0 2390 248 2494 846.0 0 2494 249 3143 846.0 0 3143 250 2944 846.0 0 2944 251 2779 846.0 0 2779 252 3447 846.0 0 3447 253 3098 846.0 0 3098 254 2111 846.0 0 2111 255 1518 846.0 0 1518 256 1426 846.0 0 1426 257 1626 846.0 0 1626 258 1989 846.0 0 1989 259 2634 846.0 0 2634 260 2830 846.0 0 2830 261 3427 846.0 0 3427 262 5264 846.0 0 5264 263 12045 846.0 0 12045 264 23996 846.0 0 23996 265 141991 846.0 0 141991 266 9180 846.0 0 9180 267 2528 846.0 0 2528 268 4022 846.0 0 4022 269 2568 846.0 0 2568 270 2368 846.0 0 2368 271 2558 846.0 0 2558 272 2944 846.0 0 2944 273 2066 846.0 0 2066 274 2357 846.0 0 2357 275 3733 846.0 0 3733 276 3346 846.0 0 3346 277 2408 846.0 0 2408 278 2983 846.0 0 2983 279 3090 846.0 0 3090 280 2920 846.0 0 2920 281 2569 846.0 0 2569 282 2091 846.0 0 2091 283 2953 846.0 0 2953 284 3036 846.0 0 3036 285 2516 846.0 0 2516 286 2567 846.0 0 2567 287 3054 846.0 0 3054 288 6108 846.0 0 6108 289 2707 846.0 0 2707 290 3778 846.0 0 3778 291 3332 846.0 0 3332 292 3526 846.0 0 3526 293 2404 846.0 0 2404 294 2006 846.0 0 2006 295 1936 846.0 0 1936 296 2410 846.0 0 2410 297 2668 846.0 0 2668 298 2543 846.0 0 2543 299 2353 846.0 0 2353 300 3058 846.0 0 3058 301 2200 846.0 0 2200 Finished in 13.00 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 378,004 Reads with adapters: 1,497 (0.4%) Reads that were too short: 185 (0.0%) Reads written (passing filters): 1,312 (0.3%) Total basepairs processed: 63,647,227 bp Total written (filtered): 341,606 bp (0.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1497 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 1 19 12 0.0 3 0 10 0 1 1 20 976 0.0 4 943 24 4 2 3 21 4 0.0 4 0 1 1 1 1 22 5 0.0 4 1 0 0 1 3 23 187 0.0 4 173 11 0 1 2 24 5 0.0 4 0 1 0 0 4 25 3 0.0 4 0 0 0 0 3 26 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 28 4 0.0 4 0 0 0 0 4 29 1 0.0 4 0 0 0 0 1 30 5 0.0 4 0 0 0 0 5 31 3 0.0 4 0 0 0 0 3 32 2 0.0 4 0 0 0 0 2 33 2 0.0 4 0 0 0 0 2 34 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 36 2 0.0 4 0 0 0 0 2 37 3 0.0 4 0 0 0 0 3 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 3 0.0 4 0 0 0 0 3 42 2 0.0 4 0 0 0 0 2 43 2 0.0 4 0 0 0 0 2 44 4 0.0 4 0 0 0 0 4 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 3 0.0 4 0 0 0 0 3 49 2 0.0 4 0 0 0 0 2 50 2 0.0 4 0 0 0 0 2 51 2 0.0 4 0 0 0 0 2 52 6 0.0 4 0 0 0 3 3 53 2 0.0 4 0 0 0 0 2 54 2 0.0 4 0 0 0 0 2 55 7 0.0 4 0 0 0 5 2 56 2 0.0 4 0 0 0 0 2 57 2 0.0 4 0 0 0 0 2 58 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 61 2 0.0 4 0 0 0 0 2 63 2 0.0 4 0 0 0 0 2 64 3 0.0 4 0 0 0 0 3 65 2 0.0 4 0 0 0 0 2 66 2 0.0 4 0 0 0 0 2 67 2 0.0 4 0 0 0 0 2 68 3 0.0 4 0 0 0 0 3 70 2 0.0 4 0 0 0 0 2 71 2 0.0 4 0 0 0 0 2 72 6 0.0 4 0 0 0 0 6 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 3 0.0 4 0 0 0 0 3 76 6 0.0 4 0 0 0 0 6 77 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 84 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 87 3 0.0 4 0 0 0 0 3 88 2 0.0 4 0 0 0 0 2 90 4 0.0 4 0 0 0 0 4 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 2 0.0 4 0 0 0 0 2 95 4 0.0 4 0 0 0 0 4 96 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 99 1 0.0 4 0 0 0 0 1 100 3 0.0 4 0 0 0 0 3 101 3 0.0 4 0 0 0 0 3 102 3 0.0 4 0 0 0 0 3 103 1 0.0 4 0 0 0 0 1 104 3 0.0 4 0 0 0 0 3 106 3 0.0 4 0 0 0 0 3 107 4 0.0 4 0 0 0 0 4 108 3 0.0 4 0 0 0 0 3 109 2 0.0 4 0 0 0 0 2 110 4 0.0 4 0 0 0 0 4 111 24 0.0 4 0 0 0 22 2 112 3 0.0 4 0 0 0 0 3 113 2 0.0 4 0 0 0 0 2 114 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 117 2 0.0 4 0 0 0 0 2 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 120 3 0.0 4 0 0 0 0 3 121 3 0.0 4 0 0 0 0 3 124 3 0.0 4 0 0 0 0 3 125 3 0.0 4 0 0 0 0 3 126 3 0.0 4 0 0 0 0 3 127 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 146 3 0.0 4 0 0 0 0 3 147 2 0.0 4 0 0 0 0 2 150 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 3 0.0 4 0 0 0 1 2 156 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 175 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 184 2 0.0 4 0 0 0 0 2 188 2 0.0 4 0 0 0 0 2 189 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 2 0.0 4 0 0 0 1 1 206 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 1,312 Reads with adapters: 136 (10.4%) Reads written (passing filters): 1,312 (100.0%) Total basepairs processed: 341,606 bp Total written (filtered): 335,597 bp (98.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 136 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 3.7% G: 86.8% T: 6.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 3 0.0 0 0 3 10 10 0.0 1 0 10 11 105 0.0 1 0 105 258 5 0.0 1 5 259 1 0.0 1 1 262 2 0.0 1 2 263 6 0.0 1 6 264 1 0.0 1 1 270 2 0.0 1 2 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.