This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.20 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 84,952 (2.5%) Reads that were too short: 37,876 (1.1%) Reads written (passing filters): 47,076 (1.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 7,230,381 bp (0.7%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 84952 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 26 846.0 0 26 7 75 846.0 0 75 8 21 846.0 0 21 9 954 846.0 0 954 10 15 846.0 0 15 11 17 846.0 0 17 12 18 846.0 0 18 13 18 846.0 0 18 14 16 846.0 0 16 15 14 846.0 0 14 16 18 846.0 0 18 17 8 846.0 0 8 18 17 846.0 0 17 19 14 846.0 0 14 20 22 846.0 0 22 21 18 846.0 0 18 22 21 846.0 0 21 23 30 846.0 0 30 24 20 846.0 0 20 25 17 846.0 0 17 26 14 846.0 0 14 27 18 846.0 0 18 28 17 846.0 0 17 29 17 846.0 0 17 30 19 846.0 0 19 31 12 846.0 0 12 32 17 846.0 0 17 33 17 846.0 0 17 34 53 846.0 0 53 35 19 846.0 0 19 36 22 846.0 0 22 37 25 846.0 0 25 38 43 846.0 0 43 39 61 846.0 0 61 40 41 846.0 0 41 41 24 846.0 0 24 42 27 846.0 0 27 43 44 846.0 0 44 44 12 846.0 0 12 45 50 846.0 0 50 46 11 846.0 0 11 47 12 846.0 0 12 48 16 846.0 0 16 49 12 846.0 0 12 50 13 846.0 0 13 51 9 846.0 0 9 52 18 846.0 0 18 53 107 846.0 0 107 54 74 846.0 0 74 55 41 846.0 0 41 56 21 846.0 0 21 57 18 846.0 0 18 58 15 846.0 0 15 59 38 846.0 0 38 60 21 846.0 0 21 61 23 846.0 0 23 62 9 846.0 0 9 63 19 846.0 0 19 64 18 846.0 0 18 65 18 846.0 0 18 66 25 846.0 0 25 67 39 846.0 0 39 68 29 846.0 0 29 69 8 846.0 0 8 70 57 846.0 0 57 71 17 846.0 0 17 72 19 846.0 0 19 73 25 846.0 0 25 74 12 846.0 0 12 75 13 846.0 0 13 76 15 846.0 0 15 77 11 846.0 0 11 78 17 846.0 0 17 79 15 846.0 0 15 80 11 846.0 0 11 81 26 846.0 0 26 82 29 846.0 0 29 83 52 846.0 0 52 84 39 846.0 0 39 85 31 846.0 0 31 86 32 846.0 0 32 87 49 846.0 0 49 88 52 846.0 0 52 89 37 846.0 0 37 90 44 846.0 0 44 91 120 846.0 0 120 92 49 846.0 0 49 93 165 846.0 0 165 94 168 846.0 0 168 95 94 846.0 0 94 96 43 846.0 0 43 97 44 846.0 0 44 98 70 846.0 0 70 99 60 846.0 0 60 100 52 846.0 0 52 101 124 846.0 0 124 102 109 846.0 0 109 103 186 846.0 0 186 104 147 846.0 0 147 105 150 846.0 0 150 106 104 846.0 0 104 107 231 846.0 0 231 108 75 846.0 0 75 109 71 846.0 0 71 110 46 846.0 0 46 111 332 846.0 0 332 112 296 846.0 0 296 113 400 846.0 0 400 114 253 846.0 0 253 115 171 846.0 0 171 116 65 846.0 0 65 117 56 846.0 0 56 118 72 846.0 0 72 119 68 846.0 0 68 120 102 846.0 0 102 121 74 846.0 0 74 122 71 846.0 0 71 123 116 846.0 0 116 124 143 846.0 0 143 125 1319 846.0 0 1319 126 559 846.0 0 559 127 1099 846.0 0 1099 128 110 846.0 0 110 129 133 846.0 0 133 130 244 846.0 0 244 131 147 846.0 0 147 132 185 846.0 0 185 133 197 846.0 0 197 134 394 846.0 0 394 135 135 846.0 0 135 136 86 846.0 0 86 137 147 846.0 0 147 138 236 846.0 0 236 139 234 846.0 0 234 140 194 846.0 0 194 141 169 846.0 0 169 142 122 846.0 0 122 143 152 846.0 0 152 144 432 846.0 0 432 145 468 846.0 0 468 146 493 846.0 0 493 147 261 846.0 0 261 148 170 846.0 0 170 149 213 846.0 0 213 150 539 846.0 0 539 151 1855 846.0 0 1855 152 462 846.0 0 462 153 248 846.0 0 248 154 335 846.0 0 335 155 2270 846.0 0 2270 156 468 846.0 0 468 157 550 846.0 0 550 158 507 846.0 0 507 159 704 846.0 0 704 160 5270 846.0 0 5270 161 3471 846.0 0 3471 162 2940 846.0 0 2940 163 319 846.0 0 319 164 517 846.0 0 517 165 476 846.0 0 476 166 302 846.0 0 302 167 177 846.0 0 177 168 172 846.0 0 172 169 257 846.0 0 257 170 211 846.0 0 211 171 211 846.0 0 211 172 192 846.0 0 192 173 189 846.0 0 189 174 185 846.0 0 185 175 219 846.0 0 219 176 207 846.0 0 207 177 278 846.0 0 278 178 309 846.0 0 309 179 387 846.0 0 387 180 1411 846.0 0 1411 181 908 846.0 0 908 182 799 846.0 0 799 183 299 846.0 0 299 184 195 846.0 0 195 185 232 846.0 0 232 186 485 846.0 0 485 187 289 846.0 0 289 188 232 846.0 0 232 189 265 846.0 0 265 190 336 846.0 0 336 191 276 846.0 0 276 192 149 846.0 0 149 193 124 846.0 0 124 194 94 846.0 0 94 195 128 846.0 0 128 196 165 846.0 0 165 197 185 846.0 0 185 198 173 846.0 0 173 199 208 846.0 0 208 200 186 846.0 0 186 201 356 846.0 0 356 202 166 846.0 0 166 203 117 846.0 0 117 204 146 846.0 0 146 205 154 846.0 0 154 206 183 846.0 0 183 207 188 846.0 0 188 208 200 846.0 0 200 209 185 846.0 0 185 210 217 846.0 0 217 211 529 846.0 0 529 212 1424 846.0 0 1424 213 945 846.0 0 945 214 1090 846.0 0 1090 215 238 846.0 0 238 216 395 846.0 0 395 217 333 846.0 0 333 218 355 846.0 0 355 219 235 846.0 0 235 220 293 846.0 0 293 221 319 846.0 0 319 222 240 846.0 0 240 223 258 846.0 0 258 224 283 846.0 0 283 225 537 846.0 0 537 226 272 846.0 0 272 227 348 846.0 0 348 228 253 846.0 0 253 229 352 846.0 0 352 230 304 846.0 0 304 231 210 846.0 0 210 232 197 846.0 0 197 233 236 846.0 0 236 234 256 846.0 0 256 235 214 846.0 0 214 236 278 846.0 0 278 237 352 846.0 0 352 238 347 846.0 0 347 239 638 846.0 0 638 240 610 846.0 0 610 241 552 846.0 0 552 242 335 846.0 0 335 243 498 846.0 0 498 244 398 846.0 0 398 245 444 846.0 0 444 246 417 846.0 0 417 247 376 846.0 0 376 248 234 846.0 0 234 249 194 846.0 0 194 250 357 846.0 0 357 251 243 846.0 0 243 252 165 846.0 0 165 253 285 846.0 0 285 254 478 846.0 0 478 255 523 846.0 0 523 256 762 846.0 0 762 257 823 846.0 0 823 258 533 846.0 0 533 259 296 846.0 0 296 260 395 846.0 0 395 261 363 846.0 0 363 262 337 846.0 0 337 263 287 846.0 0 287 264 376 846.0 0 376 265 350 846.0 0 350 266 968 846.0 0 968 267 611 846.0 0 611 268 537 846.0 0 537 269 383 846.0 0 383 270 415 846.0 0 415 271 362 846.0 0 362 272 358 846.0 0 358 273 701 846.0 0 701 274 639 846.0 0 639 275 569 846.0 0 569 276 497 846.0 0 497 277 682 846.0 0 682 278 450 846.0 0 450 279 324 846.0 0 324 280 365 846.0 0 365 281 498 846.0 0 498 282 836 846.0 0 836 283 331 846.0 0 331 284 363 846.0 0 363 285 369 846.0 0 369 286 288 846.0 0 288 287 432 846.0 0 432 288 421 846.0 0 421 289 608 846.0 0 608 290 296 846.0 0 296 291 194 846.0 0 194 292 118 846.0 0 118 293 123 846.0 0 123 294 94 846.0 0 94 295 120 846.0 0 120 296 108 846.0 0 108 297 137 846.0 0 137 298 205 846.0 0 205 299 179 846.0 0 179 300 207 846.0 0 207 301 170 846.0 0 170 Finished in 1.44 s (31 us/read; 1.96 M reads/minute). === Summary === Total reads processed: 47,076 Reads with adapters: 941 (2.0%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 927 (2.0%) Total basepairs processed: 7,230,381 bp Total written (filtered): 254,261 bp (3.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 941 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 5 0.0 3 0 4 1 17 909 0.0 3 888 19 1 1 18 6 0.0 3 1 3 2 19 1 0.0 3 0 0 0 1 20 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 1 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 99 1 0.0 3 0 1 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 927 Reads with adapters: 26 (2.8%) Reads written (passing filters): 927 (100.0%) Total basepairs processed: 254,261 bp Total written (filtered): 254,183 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 26 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.6% C: 23.1% G: 42.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 14.5 0 26