This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 87.75 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 52,363 (1.5%) Reads that were too short: 4,094 (0.1%) Reads written (passing filters): 48,269 (1.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 11,772,119 bp (1.1%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 52363 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 354 846.0 0 354 7 1134 846.0 0 1134 8 5327 846.0 0 5327 9 25 846.0 0 25 10 14 846.0 0 14 11 9 846.0 0 9 12 11 846.0 0 11 13 8 846.0 0 8 14 11 846.0 0 11 15 15 846.0 0 15 16 4 846.0 0 4 17 9 846.0 0 9 18 18 846.0 0 18 19 19 846.0 0 19 20 18 846.0 0 18 21 15 846.0 0 15 22 17 846.0 0 17 23 8 846.0 0 8 24 11 846.0 0 11 25 15 846.0 0 15 26 15 846.0 0 15 27 22 846.0 0 22 28 51 846.0 0 51 29 12 846.0 0 12 30 17 846.0 0 17 31 9 846.0 0 9 32 8 846.0 0 8 33 20 846.0 0 20 34 13 846.0 0 13 35 23 846.0 0 23 36 23 846.0 0 23 37 36 846.0 0 36 38 294 846.0 0 294 39 632 846.0 0 632 40 62 846.0 0 62 41 338 846.0 0 338 42 44 846.0 0 44 43 146 846.0 0 146 44 60 846.0 0 60 45 42 846.0 0 42 46 114 846.0 0 114 47 321 846.0 0 321 48 63 846.0 0 63 49 412 846.0 0 412 50 89 846.0 0 89 51 149 846.0 0 149 52 186 846.0 0 186 53 429 846.0 0 429 54 533 846.0 0 533 55 13710 846.0 0 13710 56 6539 846.0 0 6539 57 8072 846.0 0 8072 58 50 846.0 0 50 59 12 846.0 0 12 60 9 846.0 0 9 61 13 846.0 0 13 62 12 846.0 0 12 63 93 846.0 0 93 64 11 846.0 0 11 65 11 846.0 0 11 66 3 846.0 0 3 67 22 846.0 0 22 68 20 846.0 0 20 69 14 846.0 0 14 70 16 846.0 0 16 71 12 846.0 0 12 72 21 846.0 0 21 73 31 846.0 0 31 74 20 846.0 0 20 75 17 846.0 0 17 76 18 846.0 0 18 77 19 846.0 0 19 78 11 846.0 0 11 79 13 846.0 0 13 80 23 846.0 0 23 81 50 846.0 0 50 82 34 846.0 0 34 83 74 846.0 0 74 84 60 846.0 0 60 85 2386 846.0 0 2386 86 735 846.0 0 735 87 1821 846.0 0 1821 88 20 846.0 0 20 89 49 846.0 0 49 90 19 846.0 0 19 91 9 846.0 0 9 92 10 846.0 0 10 93 16 846.0 0 16 94 52 846.0 0 52 95 4 846.0 0 4 96 11 846.0 0 11 97 10 846.0 0 10 98 6 846.0 0 6 99 13 846.0 0 13 100 4 846.0 0 4 101 15 846.0 0 15 102 75 846.0 0 75 103 101 846.0 0 101 104 27 846.0 0 27 105 8 846.0 0 8 106 8 846.0 0 8 107 11 846.0 0 11 108 5 846.0 0 5 109 9 846.0 0 9 110 17 846.0 0 17 111 28 846.0 0 28 112 7 846.0 0 7 113 12 846.0 0 12 114 10 846.0 0 10 115 12 846.0 0 12 116 20 846.0 0 20 117 13 846.0 0 13 118 33 846.0 0 33 119 18 846.0 0 18 120 10 846.0 0 10 121 13 846.0 0 13 122 8 846.0 0 8 123 27 846.0 0 27 124 18 846.0 0 18 125 11 846.0 0 11 126 107 846.0 0 107 127 129 846.0 0 129 128 101 846.0 0 101 129 17 846.0 0 17 130 36 846.0 0 36 131 46 846.0 0 46 132 27 846.0 0 27 133 43 846.0 0 43 134 14 846.0 0 14 135 15 846.0 0 15 136 12 846.0 0 12 137 95 846.0 0 95 138 44 846.0 0 44 139 112 846.0 0 112 140 16 846.0 0 16 141 13 846.0 0 13 142 15 846.0 0 15 143 16 846.0 0 16 144 14 846.0 0 14 145 10 846.0 0 10 146 26 846.0 0 26 147 6 846.0 0 6 148 25 846.0 0 25 149 17 846.0 0 17 150 13 846.0 0 13 151 36 846.0 0 36 152 17 846.0 0 17 153 11 846.0 0 11 154 22 846.0 0 22 155 7 846.0 0 7 156 12 846.0 0 12 157 82 846.0 0 82 158 33 846.0 0 33 159 46 846.0 0 46 160 9 846.0 0 9 161 13 846.0 0 13 162 72 846.0 0 72 163 21 846.0 0 21 164 25 846.0 0 25 165 20 846.0 0 20 166 21 846.0 0 21 167 55 846.0 0 55 168 26 846.0 0 26 169 38 846.0 0 38 170 15 846.0 0 15 171 17 846.0 0 17 172 26 846.0 0 26 173 17 846.0 0 17 174 14 846.0 0 14 175 15 846.0 0 15 176 10 846.0 0 10 177 7 846.0 0 7 178 9 846.0 0 9 179 7 846.0 0 7 180 7 846.0 0 7 181 15 846.0 0 15 182 21 846.0 0 21 183 21 846.0 0 21 184 23 846.0 0 23 185 19 846.0 0 19 186 13 846.0 0 13 187 105 846.0 0 105 188 91 846.0 0 91 189 39 846.0 0 39 190 54 846.0 0 54 191 52 846.0 0 52 192 22 846.0 0 22 193 16 846.0 0 16 194 19 846.0 0 19 195 13 846.0 0 13 196 12 846.0 0 12 197 6 846.0 0 6 198 15 846.0 0 15 199 17 846.0 0 17 200 104 846.0 0 104 201 17 846.0 0 17 202 10 846.0 0 10 203 9 846.0 0 9 204 11 846.0 0 11 205 24 846.0 0 24 206 25 846.0 0 25 207 24 846.0 0 24 208 14 846.0 0 14 209 7 846.0 0 7 210 11 846.0 0 11 211 12 846.0 0 12 212 7 846.0 0 7 213 28 846.0 0 28 214 22 846.0 0 22 215 18 846.0 0 18 216 22 846.0 0 22 217 26 846.0 0 26 218 26 846.0 0 26 219 120 846.0 0 120 220 615 846.0 0 615 221 30 846.0 0 30 222 13 846.0 0 13 223 14 846.0 0 14 224 20 846.0 0 20 225 16 846.0 0 16 226 46 846.0 0 46 227 17 846.0 0 17 228 11 846.0 0 11 229 18 846.0 0 18 230 9 846.0 0 9 231 10 846.0 0 10 232 9 846.0 0 9 233 20 846.0 0 20 234 15 846.0 0 15 235 12 846.0 0 12 236 20 846.0 0 20 237 11 846.0 0 11 238 19 846.0 0 19 239 34 846.0 0 34 240 29 846.0 0 29 241 16 846.0 0 16 242 11 846.0 0 11 243 14 846.0 0 14 244 12 846.0 0 12 245 27 846.0 0 27 246 29 846.0 0 29 247 47 846.0 0 47 248 4 846.0 0 4 249 15 846.0 0 15 250 11 846.0 0 11 251 15 846.0 0 15 252 20 846.0 0 20 253 26 846.0 0 26 254 14 846.0 0 14 255 12 846.0 0 12 256 21 846.0 0 21 257 70 846.0 0 70 258 25 846.0 0 25 259 26 846.0 0 26 260 26 846.0 0 26 261 17 846.0 0 17 262 20 846.0 0 20 263 19 846.0 0 19 264 27 846.0 0 27 265 24 846.0 0 24 266 47 846.0 0 47 267 15 846.0 0 15 268 14 846.0 0 14 269 16 846.0 0 16 270 7 846.0 0 7 271 9 846.0 0 9 272 15 846.0 0 15 273 12 846.0 0 12 274 36 846.0 0 36 275 23 846.0 0 23 276 416 846.0 0 416 277 52 846.0 0 52 278 521 846.0 0 521 279 19 846.0 0 19 280 30 846.0 0 30 281 92 846.0 0 92 282 28 846.0 0 28 283 34 846.0 0 34 284 19 846.0 0 19 285 45 846.0 0 45 286 16 846.0 0 16 287 27 846.0 0 27 288 23 846.0 0 23 289 28 846.0 0 28 290 9 846.0 0 9 291 17 846.0 0 17 292 23 846.0 0 23 293 6 846.0 0 6 294 48 846.0 0 48 295 27 846.0 0 27 296 126 846.0 0 126 297 95 846.0 0 95 298 42 846.0 0 42 299 93 846.0 0 93 300 83 846.0 0 83 301 29 846.0 0 29 Finished in 1.99 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 48,269 Reads with adapters: 1,091 (2.3%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 1,081 (2.2%) Total basepairs processed: 11,772,119 bp Total written (filtered): 298,955 bp (2.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1091 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 3 0.0 3 0 3 17 1071 0.0 3 1059 11 1 18 3 0.0 3 0 3 19 1 0.0 3 0 0 1 28 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (28 us/read; 2.16 M reads/minute). === Summary === Total reads processed: 1,081 Reads with adapters: 105 (9.7%) Reads written (passing filters): 1,081 (100.0%) Total basepairs processed: 298,955 bp Total written (filtered): 298,640 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 105 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.9% C: 95.2% G: 1.9% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 105 16.9 0 105 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.