This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 87.43 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 84,911 (2.5%) Reads that were too short: 14,959 (0.4%) Reads written (passing filters): 69,952 (2.0%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 14,311,420 bp (1.4%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 84911 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 152 846.0 0 152 7 111 846.0 0 111 8 166 846.0 0 166 9 8078 846.0 0 8078 10 60 846.0 0 60 11 46 846.0 0 46 12 45 846.0 0 45 13 99 846.0 0 99 14 49 846.0 0 49 15 32 846.0 0 32 16 26 846.0 0 26 17 618 846.0 0 618 18 54 846.0 0 54 19 26 846.0 0 26 20 23 846.0 0 23 21 29 846.0 0 29 22 38 846.0 0 38 23 27 846.0 0 27 24 22 846.0 0 22 25 71 846.0 0 71 26 48 846.0 0 48 27 36 846.0 0 36 28 22 846.0 0 22 29 110 846.0 0 110 30 38 846.0 0 38 31 174 846.0 0 174 32 668 846.0 0 668 33 172 846.0 0 172 34 55 846.0 0 55 35 72 846.0 0 72 36 21 846.0 0 21 37 27 846.0 0 27 38 40 846.0 0 40 39 47 846.0 0 47 40 31 846.0 0 31 41 131 846.0 0 131 42 121 846.0 0 121 43 27 846.0 0 27 44 31 846.0 0 31 45 35 846.0 0 35 46 32 846.0 0 32 47 60 846.0 0 60 48 274 846.0 0 274 49 42 846.0 0 42 50 65 846.0 0 65 51 47 846.0 0 47 52 59 846.0 0 59 53 45 846.0 0 45 54 35 846.0 0 35 55 101 846.0 0 101 56 548 846.0 0 548 57 131 846.0 0 131 58 38 846.0 0 38 59 62 846.0 0 62 60 49 846.0 0 49 61 209 846.0 0 209 62 61 846.0 0 61 63 37 846.0 0 37 64 35 846.0 0 35 65 316 846.0 0 316 66 1129 846.0 0 1129 67 171 846.0 0 171 68 25 846.0 0 25 69 52 846.0 0 52 70 69 846.0 0 69 71 84 846.0 0 84 72 157 846.0 0 157 73 79 846.0 0 79 74 44 846.0 0 44 75 58 846.0 0 58 76 130 846.0 0 130 77 188 846.0 0 188 78 116 846.0 0 116 79 82 846.0 0 82 80 70 846.0 0 70 81 116 846.0 0 116 82 168 846.0 0 168 83 172 846.0 0 172 84 517 846.0 0 517 85 333 846.0 0 333 86 2045 846.0 0 2045 87 1483 846.0 0 1483 88 1109 846.0 0 1109 89 275 846.0 0 275 90 609 846.0 0 609 91 102 846.0 0 102 92 222 846.0 0 222 93 451 846.0 0 451 94 316 846.0 0 316 95 96 846.0 0 96 96 98 846.0 0 98 97 151 846.0 0 151 98 188 846.0 0 188 99 175 846.0 0 175 100 189 846.0 0 189 101 324 846.0 0 324 102 404 846.0 0 404 103 9355 846.0 0 9355 104 13726 846.0 0 13726 105 3478 846.0 0 3478 106 233 846.0 0 233 107 275 846.0 0 275 108 141 846.0 0 141 109 41 846.0 0 41 110 94 846.0 0 94 111 133 846.0 0 133 112 119 846.0 0 119 113 112 846.0 0 112 114 100 846.0 0 100 115 83 846.0 0 83 116 35 846.0 0 35 117 60 846.0 0 60 118 121 846.0 0 121 119 390 846.0 0 390 120 160 846.0 0 160 121 78 846.0 0 78 122 92 846.0 0 92 123 71 846.0 0 71 124 189 846.0 0 189 125 273 846.0 0 273 126 261 846.0 0 261 127 197 846.0 0 197 128 101 846.0 0 101 129 85 846.0 0 85 130 87 846.0 0 87 131 91 846.0 0 91 132 260 846.0 0 260 133 398 846.0 0 398 134 312 846.0 0 312 135 138 846.0 0 138 136 130 846.0 0 130 137 128 846.0 0 128 138 311 846.0 0 311 139 697 846.0 0 697 140 455 846.0 0 455 141 170 846.0 0 170 142 80 846.0 0 80 143 230 846.0 0 230 144 373 846.0 0 373 145 332 846.0 0 332 146 149 846.0 0 149 147 94 846.0 0 94 148 120 846.0 0 120 149 201 846.0 0 201 150 318 846.0 0 318 151 221 846.0 0 221 152 198 846.0 0 198 153 334 846.0 0 334 154 254 846.0 0 254 155 204 846.0 0 204 156 138 846.0 0 138 157 148 846.0 0 148 158 271 846.0 0 271 159 250 846.0 0 250 160 243 846.0 0 243 161 114 846.0 0 114 162 67 846.0 0 67 163 127 846.0 0 127 164 264 846.0 0 264 165 258 846.0 0 258 166 121 846.0 0 121 167 89 846.0 0 89 168 150 846.0 0 150 169 100 846.0 0 100 170 101 846.0 0 101 171 120 846.0 0 120 172 406 846.0 0 406 173 342 846.0 0 342 174 109 846.0 0 109 175 62 846.0 0 62 176 75 846.0 0 75 177 93 846.0 0 93 178 332 846.0 0 332 179 258 846.0 0 258 180 359 846.0 0 359 181 169 846.0 0 169 182 107 846.0 0 107 183 91 846.0 0 91 184 250 846.0 0 250 185 403 846.0 0 403 186 188 846.0 0 188 187 101 846.0 0 101 188 108 846.0 0 108 189 260 846.0 0 260 190 336 846.0 0 336 191 217 846.0 0 217 192 97 846.0 0 97 193 76 846.0 0 76 194 76 846.0 0 76 195 64 846.0 0 64 196 56 846.0 0 56 197 77 846.0 0 77 198 94 846.0 0 94 199 116 846.0 0 116 200 126 846.0 0 126 201 134 846.0 0 134 202 94 846.0 0 94 203 115 846.0 0 115 204 156 846.0 0 156 205 320 846.0 0 320 206 464 846.0 0 464 207 583 846.0 0 583 208 566 846.0 0 566 209 171 846.0 0 171 210 170 846.0 0 170 211 255 846.0 0 255 212 197 846.0 0 197 213 237 846.0 0 237 214 182 846.0 0 182 215 233 846.0 0 233 216 155 846.0 0 155 217 148 846.0 0 148 218 143 846.0 0 143 219 247 846.0 0 247 220 251 846.0 0 251 221 231 846.0 0 231 222 75 846.0 0 75 223 253 846.0 0 253 224 228 846.0 0 228 225 284 846.0 0 284 226 165 846.0 0 165 227 179 846.0 0 179 228 126 846.0 0 126 229 170 846.0 0 170 230 128 846.0 0 128 231 112 846.0 0 112 232 72 846.0 0 72 233 198 846.0 0 198 234 181 846.0 0 181 235 169 846.0 0 169 236 112 846.0 0 112 237 76 846.0 0 76 238 74 846.0 0 74 239 65 846.0 0 65 240 78 846.0 0 78 241 95 846.0 0 95 242 102 846.0 0 102 243 74 846.0 0 74 244 89 846.0 0 89 245 89 846.0 0 89 246 98 846.0 0 98 247 106 846.0 0 106 248 48 846.0 0 48 249 31 846.0 0 31 250 54 846.0 0 54 251 64 846.0 0 64 252 85 846.0 0 85 253 130 846.0 0 130 254 177 846.0 0 177 255 218 846.0 0 218 256 327 846.0 0 327 257 159 846.0 0 159 258 104 846.0 0 104 259 297 846.0 0 297 260 330 846.0 0 330 261 365 846.0 0 365 262 505 846.0 0 505 263 686 846.0 0 686 264 93 846.0 0 93 265 190 846.0 0 190 266 204 846.0 0 204 267 85 846.0 0 85 268 46 846.0 0 46 269 26 846.0 0 26 270 47 846.0 0 47 271 47 846.0 0 47 272 42 846.0 0 42 273 62 846.0 0 62 274 64 846.0 0 64 275 67 846.0 0 67 276 48 846.0 0 48 277 54 846.0 0 54 278 78 846.0 0 78 279 656 846.0 0 656 280 125 846.0 0 125 281 122 846.0 0 122 282 78 846.0 0 78 283 65 846.0 0 65 284 129 846.0 0 129 285 56 846.0 0 56 286 52 846.0 0 52 287 62 846.0 0 62 288 46 846.0 0 46 289 23 846.0 0 23 290 27 846.0 0 27 291 18 846.0 0 18 292 39 846.0 0 39 293 59 846.0 0 59 294 35 846.0 0 35 295 32 846.0 0 32 296 48 846.0 0 48 297 23 846.0 0 23 298 32 846.0 0 32 299 38 846.0 0 38 300 51 846.0 0 51 301 94 846.0 0 94 Finished in 2.85 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 69,952 Reads with adapters: 8,166 (11.7%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 8,159 (11.7%) Total basepairs processed: 14,311,420 bp Total written (filtered): 2,219,029 bp (15.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8166 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 29 0.0 3 0 26 1 1 1 20 8097 0.0 4 7648 400 36 11 2 21 22 0.0 4 3 17 1 0 1 22 3 0.0 4 0 0 3 23 4 0.0 4 0 0 0 4 59 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 1 152 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 8,159 Reads with adapters: 405 (5.0%) Reads written (passing filters): 8,159 (100.0%) Total basepairs processed: 2,219,029 bp Total written (filtered): 2,213,807 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 405 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.4% C: 4.0% G: 86.9% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 127.5 0 1 4 1 31.9 0 1 8 1 0.1 0 1 9 70 0.0 0 7 63 10 250 0.0 1 7 243 11 76 0.0 1 0 76 13 1 0.0 1 0 1 169 1 0.0 1 1 261 1 0.0 1 1 262 1 0.0 1 1 266 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.