This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 92.71 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 1,259,969 (36.4%) Reads that were too short: 112,072 (3.2%) Reads written (passing filters): 1,147,897 (33.1%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 311,038,046 bp (29.8%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 1259969 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 74 846.0 0 74 7 175 846.0 0 175 8 8992 846.0 0 8992 9 63 846.0 0 63 10 32 846.0 0 32 11 59 846.0 0 59 12 32 846.0 0 32 13 27 846.0 0 27 14 39 846.0 0 39 15 66 846.0 0 66 16 75 846.0 0 75 17 29 846.0 0 29 18 59 846.0 0 59 19 162 846.0 0 162 20 855138 846.0 0 855138 21 72614 846.0 0 72614 22 292 846.0 0 292 23 77 846.0 0 77 24 123 846.0 0 123 25 42 846.0 0 42 26 460 846.0 0 460 27 71 846.0 0 71 28 35 846.0 0 35 29 51 846.0 0 51 30 40 846.0 0 40 31 74 846.0 0 74 32 214 846.0 0 214 33 55931 846.0 0 55931 34 2018 846.0 0 2018 35 128 846.0 0 128 36 40 846.0 0 40 37 41 846.0 0 41 38 72 846.0 0 72 39 78 846.0 0 78 40 72 846.0 0 72 41 88 846.0 0 88 42 272 846.0 0 272 43 553 846.0 0 553 44 297 846.0 0 297 45 73 846.0 0 73 46 188 846.0 0 188 47 76 846.0 0 76 48 69 846.0 0 69 49 84 846.0 0 84 50 161 846.0 0 161 51 276 846.0 0 276 52 102 846.0 0 102 53 632 846.0 0 632 54 134 846.0 0 134 55 380 846.0 0 380 56 240 846.0 0 240 57 215 846.0 0 215 58 355 846.0 0 355 59 262 846.0 0 262 60 272 846.0 0 272 61 259 846.0 0 259 62 320 846.0 0 320 63 276 846.0 0 276 64 266 846.0 0 266 65 288 846.0 0 288 66 324 846.0 0 324 67 391 846.0 0 391 68 414 846.0 0 414 69 526 846.0 0 526 70 633 846.0 0 633 71 571 846.0 0 571 72 670 846.0 0 670 73 956 846.0 0 956 74 1370 846.0 0 1370 75 13423 846.0 0 13423 76 27015 846.0 0 27015 77 3087 846.0 0 3087 78 9466 846.0 0 9466 79 763 846.0 0 763 80 2010 846.0 0 2010 81 839 846.0 0 839 82 1495 846.0 0 1495 83 4503 846.0 0 4503 84 12286 846.0 0 12286 85 979 846.0 0 979 86 1150 846.0 0 1150 87 17474 846.0 0 17474 88 2584 846.0 0 2584 89 1093 846.0 0 1093 90 2663 846.0 0 2663 91 5211 846.0 0 5211 92 1430 846.0 0 1430 93 312 846.0 0 312 94 268 846.0 0 268 95 310 846.0 0 310 96 357 846.0 0 357 97 455 846.0 0 455 98 283 846.0 0 283 99 301 846.0 0 301 100 681 846.0 0 681 101 371 846.0 0 371 102 271 846.0 0 271 103 294 846.0 0 294 104 299 846.0 0 299 105 235 846.0 0 235 106 236 846.0 0 236 107 246 846.0 0 246 108 211 846.0 0 211 109 235 846.0 0 235 110 215 846.0 0 215 111 221 846.0 0 221 112 234 846.0 0 234 113 232 846.0 0 232 114 280 846.0 0 280 115 298 846.0 0 298 116 249 846.0 0 249 117 246 846.0 0 246 118 179 846.0 0 179 119 208 846.0 0 208 120 249 846.0 0 249 121 200 846.0 0 200 122 221 846.0 0 221 123 526 846.0 0 526 124 262 846.0 0 262 125 262 846.0 0 262 126 260 846.0 0 260 127 217 846.0 0 217 128 252 846.0 0 252 129 246 846.0 0 246 130 493 846.0 0 493 131 417 846.0 0 417 132 658 846.0 0 658 133 261 846.0 0 261 134 275 846.0 0 275 135 230 846.0 0 230 136 1965 846.0 0 1965 137 2412 846.0 0 2412 138 1996 846.0 0 1996 139 290 846.0 0 290 140 268 846.0 0 268 141 180 846.0 0 180 142 202 846.0 0 202 143 167 846.0 0 167 144 143 846.0 0 143 145 200 846.0 0 200 146 188 846.0 0 188 147 172 846.0 0 172 148 186 846.0 0 186 149 200 846.0 0 200 150 188 846.0 0 188 151 284 846.0 0 284 152 163 846.0 0 163 153 202 846.0 0 202 154 169 846.0 0 169 155 168 846.0 0 168 156 147 846.0 0 147 157 172 846.0 0 172 158 157 846.0 0 157 159 173 846.0 0 173 160 120 846.0 0 120 161 185 846.0 0 185 162 226 846.0 0 226 163 159 846.0 0 159 164 177 846.0 0 177 165 172 846.0 0 172 166 174 846.0 0 174 167 168 846.0 0 168 168 167 846.0 0 167 169 122 846.0 0 122 170 195 846.0 0 195 171 651 846.0 0 651 172 158 846.0 0 158 173 151 846.0 0 151 174 120 846.0 0 120 175 201 846.0 0 201 176 157 846.0 0 157 177 192 846.0 0 192 178 153 846.0 0 153 179 143 846.0 0 143 180 161 846.0 0 161 181 157 846.0 0 157 182 146 846.0 0 146 183 158 846.0 0 158 184 168 846.0 0 168 185 176 846.0 0 176 186 167 846.0 0 167 187 210 846.0 0 210 188 212 846.0 0 212 189 434 846.0 0 434 190 183 846.0 0 183 191 140 846.0 0 140 192 140 846.0 0 140 193 195 846.0 0 195 194 192 846.0 0 192 195 192 846.0 0 192 196 226 846.0 0 226 197 206 846.0 0 206 198 112 846.0 0 112 199 139 846.0 0 139 200 154 846.0 0 154 201 131 846.0 0 131 202 189 846.0 0 189 203 193 846.0 0 193 204 155 846.0 0 155 205 138 846.0 0 138 206 145 846.0 0 145 207 136 846.0 0 136 208 164 846.0 0 164 209 164 846.0 0 164 210 160 846.0 0 160 211 135 846.0 0 135 212 164 846.0 0 164 213 188 846.0 0 188 214 177 846.0 0 177 215 189 846.0 0 189 216 153 846.0 0 153 217 170 846.0 0 170 218 140 846.0 0 140 219 166 846.0 0 166 220 159 846.0 0 159 221 165 846.0 0 165 222 178 846.0 0 178 223 192 846.0 0 192 224 220 846.0 0 220 225 229 846.0 0 229 226 648 846.0 0 648 227 636 846.0 0 636 228 612 846.0 0 612 229 524 846.0 0 524 230 446 846.0 0 446 231 886 846.0 0 886 232 296 846.0 0 296 233 309 846.0 0 309 234 299 846.0 0 299 235 269 846.0 0 269 236 488 846.0 0 488 237 1074 846.0 0 1074 238 946 846.0 0 946 239 301 846.0 0 301 240 276 846.0 0 276 241 408 846.0 0 408 242 911 846.0 0 911 243 566 846.0 0 566 244 449 846.0 0 449 245 510 846.0 0 510 246 670 846.0 0 670 247 813 846.0 0 813 248 1097 846.0 0 1097 249 966 846.0 0 966 250 2094 846.0 0 2094 251 69059 846.0 0 69059 252 4708 846.0 0 4708 253 11316 846.0 0 11316 254 239 846.0 0 239 255 177 846.0 0 177 256 183 846.0 0 183 257 179 846.0 0 179 258 161 846.0 0 161 259 122 846.0 0 122 260 131 846.0 0 131 261 134 846.0 0 134 262 131 846.0 0 131 263 131 846.0 0 131 264 117 846.0 0 117 265 126 846.0 0 126 266 122 846.0 0 122 267 146 846.0 0 146 268 162 846.0 0 162 269 152 846.0 0 152 270 116 846.0 0 116 271 127 846.0 0 127 272 143 846.0 0 143 273 125 846.0 0 125 274 136 846.0 0 136 275 123 846.0 0 123 276 96 846.0 0 96 277 91 846.0 0 91 278 89 846.0 0 89 279 96 846.0 0 96 280 140 846.0 0 140 281 164 846.0 0 164 282 108 846.0 0 108 283 131 846.0 0 131 284 128 846.0 0 128 285 123 846.0 0 123 286 139 846.0 0 139 287 110 846.0 0 110 288 132 846.0 0 132 289 87 846.0 0 87 290 93 846.0 0 93 291 92 846.0 0 92 292 119 846.0 0 119 293 153 846.0 0 153 294 161 846.0 0 161 295 157 846.0 0 157 296 148 846.0 0 148 297 169 846.0 0 169 298 106 846.0 0 106 299 241 846.0 0 241 300 118 846.0 0 118 301 52 846.0 0 52 Finished in 50.27 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 1,147,897 Reads with adapters: 10,233 (0.9%) Reads that were too short: 612 (0.1%) Reads written (passing filters): 9,621 (0.8%) Total basepairs processed: 311,038,046 bp Total written (filtered): 2,551,151 bp (0.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10233 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 3 0.0 3 0 0 0 2 1 19 54 0.0 3 0 38 4 6 6 20 8861 0.0 4 8583 230 38 7 3 21 31 0.0 4 3 26 1 1 23 29 0.0 4 2 0 0 19 8 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 46 1 0.0 4 0 1 54 3 0.0 4 0 0 0 0 3 60 17 0.0 4 0 0 0 0 17 61 1 0.0 4 0 0 0 1 64 2 0.0 4 0 0 0 0 2 65 5 0.0 4 0 0 0 1 4 66 3 0.0 4 0 0 0 0 3 67 3 0.0 4 0 0 0 0 3 68 2 0.0 4 0 0 0 0 2 69 5 0.0 4 0 0 0 1 4 71 4 0.0 4 0 0 0 0 4 72 1 0.0 4 0 0 0 0 1 73 6 0.0 4 0 0 0 0 6 74 5 0.0 4 0 0 0 0 5 75 3 0.0 4 0 0 0 0 3 76 4 0.0 4 0 0 0 0 4 77 5 0.0 4 0 0 0 0 5 78 19 0.0 4 0 0 0 1 18 79 23 0.0 4 0 0 0 0 23 80 29 0.0 4 0 0 1 1 27 81 13 0.0 4 0 0 0 1 12 82 15 0.0 4 0 0 0 1 14 83 6 0.0 4 0 0 0 0 6 84 7 0.0 4 0 0 0 0 7 85 7 0.0 4 0 0 0 0 7 86 1 0.0 4 0 0 0 0 1 87 4 0.0 4 0 0 0 0 4 88 2 0.0 4 0 0 0 0 2 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 3 0.0 4 0 0 0 0 3 96 8 0.0 4 0 0 0 0 8 97 3 0.0 4 0 0 0 0 3 98 7 0.0 4 0 0 0 1 6 99 22 0.0 4 0 0 0 2 20 100 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 3 0.0 4 0 0 0 0 3 106 2 0.0 4 0 0 0 0 2 107 5 0.0 4 0 0 0 0 5 108 10 0.0 4 0 0 0 1 9 109 4 0.0 4 0 0 0 1 3 110 5 0.0 4 0 0 0 0 5 111 4 0.0 4 0 0 0 0 4 112 4 0.0 4 0 0 0 0 4 113 5 0.0 4 0 0 0 0 5 114 3 0.0 4 0 0 0 0 3 115 18 0.0 4 0 0 0 1 17 116 10 0.0 4 0 0 0 0 10 117 5 0.0 4 0 0 0 0 5 118 6 0.0 4 0 0 0 0 6 119 9 0.0 4 0 0 0 0 9 120 3 0.0 4 0 0 0 0 3 121 5 0.0 4 0 0 0 0 5 122 4 0.0 4 0 0 0 0 4 123 1 0.0 4 0 0 0 0 1 124 4 0.0 4 0 0 0 0 4 125 3 0.0 4 0 0 0 0 3 126 5 0.0 4 0 0 0 0 5 127 2 0.0 4 0 0 0 0 2 128 4 0.0 4 0 0 0 0 4 129 2 0.0 4 0 0 0 0 2 130 6 0.0 4 0 0 0 0 6 131 9 0.0 4 0 0 0 0 9 132 8 0.0 4 0 0 0 0 8 133 10 0.0 4 0 0 0 0 10 134 2 0.0 4 0 0 0 1 1 135 3 0.0 4 0 0 0 0 3 136 6 0.0 4 0 0 0 0 6 137 2 0.0 4 0 0 0 0 2 138 5 0.0 4 0 0 0 0 5 139 12 0.0 4 0 0 0 1 11 140 13 0.0 4 0 0 0 1 12 141 14 0.0 4 0 0 0 2 12 142 5 0.0 4 0 0 0 0 5 143 2 0.0 4 0 0 0 0 2 144 8 0.0 4 0 0 0 0 8 145 8 0.0 4 0 0 0 1 7 146 4 0.0 4 0 0 0 0 4 147 1 0.0 4 0 0 0 0 1 148 2 0.0 4 0 0 0 0 2 149 3 0.0 4 0 0 0 0 3 150 3 0.0 4 0 0 0 0 3 151 4 0.0 4 0 0 0 0 4 152 7 0.0 4 0 0 0 0 7 153 3 0.0 4 0 0 0 0 3 154 6 0.0 4 0 0 0 1 5 155 4 0.0 4 0 0 0 1 3 156 4 0.0 4 0 0 0 0 4 157 4 0.0 4 0 0 0 0 4 158 6 0.0 4 0 0 0 0 6 159 8 0.0 4 0 0 0 0 8 160 12 0.0 4 0 0 0 0 12 161 6 0.0 4 0 0 0 0 6 162 4 0.0 4 0 0 0 0 4 163 5 0.0 4 0 0 0 0 5 164 5 0.0 4 0 0 0 0 5 165 5 0.0 4 0 0 0 0 5 166 6 0.0 4 0 0 0 0 6 167 3 0.0 4 0 0 0 0 3 168 8 0.0 4 0 0 0 0 8 169 7 0.0 4 0 0 0 1 6 170 11 0.0 4 0 0 0 0 11 171 6 0.0 4 0 0 0 0 6 172 6 0.0 4 0 0 0 0 6 173 5 0.0 4 0 0 0 0 5 174 10 0.0 4 0 0 0 1 9 175 8 0.0 4 0 0 0 0 8 176 7 0.0 4 0 0 0 0 7 177 10 0.0 4 0 0 0 0 10 178 7 0.0 4 0 0 0 1 6 179 10 0.0 4 0 0 0 1 9 180 9 0.0 4 0 0 0 0 9 181 6 0.0 4 0 0 0 0 6 182 6 0.0 4 0 0 0 0 6 183 9 0.0 4 0 0 0 0 9 184 10 0.0 4 0 0 0 0 10 185 4 0.0 4 0 0 0 0 4 186 5 0.0 4 0 0 0 0 5 187 3 0.0 4 0 0 0 0 3 188 6 0.0 4 0 0 0 0 6 189 8 0.0 4 0 0 0 0 8 190 7 0.0 4 0 0 0 0 7 191 10 0.0 4 0 0 0 0 10 192 10 0.0 4 0 0 0 0 10 193 12 0.0 4 0 0 0 0 12 194 5 0.0 4 0 0 0 0 5 195 11 0.0 4 0 0 0 0 11 196 6 0.0 4 0 0 0 0 6 197 12 0.0 4 0 0 0 0 12 198 5 0.0 4 0 0 0 0 5 199 7 0.0 4 0 0 0 0 7 200 12 0.0 4 0 0 0 0 12 201 8 0.0 4 0 0 0 0 8 202 7 0.0 4 0 0 0 0 7 203 7 0.0 4 0 0 0 0 7 204 10 0.0 4 0 0 0 0 10 205 7 0.0 4 0 0 0 0 7 206 13 0.0 4 0 0 0 0 13 207 7 0.0 4 0 0 0 0 7 208 11 0.0 4 0 0 0 0 11 209 18 0.0 4 0 0 0 0 18 210 5 0.0 4 0 0 0 0 5 211 7 0.0 4 0 0 0 0 7 212 8 0.0 4 0 0 0 0 8 213 10 0.0 4 0 0 0 0 10 214 5 0.0 4 0 0 0 0 5 215 7 0.0 4 0 0 0 0 7 216 4 0.0 4 0 0 0 0 4 217 8 0.0 4 0 0 0 0 8 218 4 0.0 4 0 0 0 0 4 219 8 0.0 4 0 0 0 0 8 220 5 0.0 4 0 0 0 0 5 221 5 0.0 4 0 0 0 0 5 222 3 0.0 4 0 0 0 0 3 223 9 0.0 4 0 0 0 0 9 224 7 0.0 4 0 0 0 1 6 225 10 0.0 4 0 0 0 0 10 226 6 0.0 4 0 0 0 0 6 227 4 0.0 4 0 0 0 0 4 228 6 0.0 4 0 0 0 0 6 229 6 0.0 4 0 0 0 0 6 230 1 0.0 4 0 0 0 0 1 231 3 0.0 4 0 0 0 0 3 232 4 0.0 4 0 0 0 0 4 233 1 0.0 4 0 0 0 0 1 234 9 0.0 4 0 0 0 0 9 235 4 0.0 4 0 0 0 0 4 236 4 0.0 4 0 0 0 0 4 237 4 0.0 4 0 0 0 0 4 238 5 0.0 4 0 0 0 0 5 239 4 0.0 4 0 0 0 0 4 240 3 0.0 4 0 0 0 0 3 241 1 0.0 4 0 0 0 0 1 242 3 0.0 4 0 0 0 1 2 243 2 0.0 4 0 0 0 0 2 244 2 0.0 4 0 0 0 0 2 245 2 0.0 4 0 0 0 0 2 246 3 0.0 4 0 0 0 0 3 247 3 0.0 4 0 0 0 1 2 248 6 0.0 4 0 0 0 0 6 249 4 0.0 4 0 0 0 0 4 250 1 0.0 4 0 0 0 0 1 251 7 0.0 4 0 0 0 0 7 252 5 0.0 4 0 0 0 0 5 253 2 0.0 4 0 0 0 0 2 254 5 0.0 4 0 0 0 0 5 255 3 0.0 4 0 0 0 0 3 256 3 0.0 4 0 0 0 0 3 257 1 0.0 4 0 0 0 0 1 258 2 0.0 4 0 0 0 0 2 259 2 0.0 4 0 0 0 0 2 260 4 0.0 4 0 0 0 0 4 261 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 263 3 0.0 4 0 0 0 0 3 264 3 0.0 4 0 0 0 0 3 265 2 0.0 4 0 0 0 0 2 266 2 0.0 4 0 0 0 0 2 267 2 0.0 4 0 0 0 0 2 268 4 0.0 4 0 0 0 0 4 269 3 0.0 4 0 0 0 0 3 270 3 0.0 4 0 0 0 0 3 271 2 0.0 4 0 0 0 0 2 272 4 0.0 4 0 0 0 1 3 273 8 0.0 4 0 0 0 0 8 274 7 0.0 4 0 0 0 1 6 275 6 0.0 4 0 0 0 0 6 276 4 0.0 4 0 0 0 0 4 277 5 0.0 4 0 0 0 1 4 278 5 0.0 4 0 0 0 0 5 279 3 0.0 4 0 0 0 0 3 280 2 0.0 4 0 0 0 0 2 281 6 0.0 4 0 0 0 0 6 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 9,621 Reads with adapters: 894 (9.3%) Reads written (passing filters): 9,621 (100.0%) Total basepairs processed: 2,551,151 bp Total written (filtered): 2,533,778 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 894 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 4.9% G: 90.2% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 150.3 0 1 4 23 37.6 0 23 6 1 2.3 0 1 9 3 0.0 0 0 3 10 74 0.0 1 5 69 11 761 0.0 1 7 754 261 22 0.0 1 20 2 262 2 0.0 1 2 263 2 0.0 1 2 264 2 0.0 1 2 270 1 0.0 1 1 272 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.