This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.01 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 207,266 (6.0%) Reads that were too short: 40,838 (1.2%) Reads written (passing filters): 166,428 (4.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 41,718,981 bp (4.0%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 207266 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 699 846.0 0 699 7 8241 846.0 0 8241 8 347 846.0 0 347 9 5240 846.0 0 5240 10 594 846.0 0 594 11 757 846.0 0 757 12 18995 846.0 0 18995 13 29186 846.0 0 29186 14 49858 846.0 0 49858 15 105 846.0 0 105 16 7 846.0 0 7 17 16 846.0 0 16 18 16 846.0 0 16 19 13 846.0 0 13 20 18 846.0 0 18 21 14 846.0 0 14 22 19 846.0 0 19 23 23 846.0 0 23 24 59 846.0 0 59 25 35 846.0 0 35 26 39 846.0 0 39 27 54 846.0 0 54 28 27 846.0 0 27 29 15 846.0 0 15 30 27 846.0 0 27 31 28 846.0 0 28 32 21 846.0 0 21 33 20 846.0 0 20 34 25 846.0 0 25 35 24 846.0 0 24 36 41 846.0 0 41 37 64 846.0 0 64 38 2418 846.0 0 2418 39 26 846.0 0 26 40 28 846.0 0 28 41 20 846.0 0 20 42 45 846.0 0 45 43 42 846.0 0 42 44 471 846.0 0 471 45 21 846.0 0 21 46 27 846.0 0 27 47 28 846.0 0 28 48 18 846.0 0 18 49 17 846.0 0 17 50 27 846.0 0 27 51 17 846.0 0 17 52 17 846.0 0 17 53 29 846.0 0 29 54 12 846.0 0 12 55 12 846.0 0 12 56 15 846.0 0 15 57 10 846.0 0 10 58 14 846.0 0 14 59 24 846.0 0 24 60 17 846.0 0 17 61 26 846.0 0 26 62 13 846.0 0 13 63 13 846.0 0 13 64 12 846.0 0 12 65 16 846.0 0 16 66 20 846.0 0 20 67 16 846.0 0 16 68 18 846.0 0 18 69 15 846.0 0 15 70 21 846.0 0 21 71 15 846.0 0 15 72 18 846.0 0 18 73 17 846.0 0 17 74 20 846.0 0 20 75 13 846.0 0 13 76 21 846.0 0 21 77 20 846.0 0 20 78 21 846.0 0 21 79 23 846.0 0 23 80 43 846.0 0 43 81 41 846.0 0 41 82 32 846.0 0 32 83 80 846.0 0 80 84 57 846.0 0 57 85 59 846.0 0 59 86 86 846.0 0 86 87 98 846.0 0 98 88 85 846.0 0 85 89 248 846.0 0 248 90 321 846.0 0 321 91 478 846.0 0 478 92 373 846.0 0 373 93 209 846.0 0 209 94 412 846.0 0 412 95 513 846.0 0 513 96 453 846.0 0 453 97 367 846.0 0 367 98 157 846.0 0 157 99 92 846.0 0 92 100 100 846.0 0 100 101 104 846.0 0 104 102 89 846.0 0 89 103 107 846.0 0 107 104 2498 846.0 0 2498 105 811 846.0 0 811 106 522 846.0 0 522 107 192 846.0 0 192 108 815 846.0 0 815 109 694 846.0 0 694 110 379 846.0 0 379 111 306 846.0 0 306 112 268 846.0 0 268 113 243 846.0 0 243 114 141 846.0 0 141 115 148 846.0 0 148 116 117 846.0 0 117 117 91 846.0 0 91 118 106 846.0 0 106 119 171 846.0 0 171 120 494 846.0 0 494 121 418 846.0 0 418 122 260 846.0 0 260 123 96 846.0 0 96 124 205 846.0 0 205 125 349 846.0 0 349 126 952 846.0 0 952 127 2351 846.0 0 2351 128 3056 846.0 0 3056 129 656 846.0 0 656 130 183 846.0 0 183 131 115 846.0 0 115 132 151 846.0 0 151 133 258 846.0 0 258 134 277 846.0 0 277 135 679 846.0 0 679 136 507 846.0 0 507 137 304 846.0 0 304 138 420 846.0 0 420 139 565 846.0 0 565 140 963 846.0 0 963 141 508 846.0 0 508 142 333 846.0 0 333 143 163 846.0 0 163 144 239 846.0 0 239 145 327 846.0 0 327 146 591 846.0 0 591 147 371 846.0 0 371 148 307 846.0 0 307 149 241 846.0 0 241 150 189 846.0 0 189 151 806 846.0 0 806 152 1911 846.0 0 1911 153 914 846.0 0 914 154 482 846.0 0 482 155 323 846.0 0 323 156 304 846.0 0 304 157 219 846.0 0 219 158 552 846.0 0 552 159 1103 846.0 0 1103 160 496 846.0 0 496 161 520 846.0 0 520 162 335 846.0 0 335 163 247 846.0 0 247 164 259 846.0 0 259 165 341 846.0 0 341 166 321 846.0 0 321 167 260 846.0 0 260 168 173 846.0 0 173 169 211 846.0 0 211 170 240 846.0 0 240 171 208 846.0 0 208 172 255 846.0 0 255 173 326 846.0 0 326 174 516 846.0 0 516 175 423 846.0 0 423 176 287 846.0 0 287 177 222 846.0 0 222 178 168 846.0 0 168 179 273 846.0 0 273 180 277 846.0 0 277 181 497 846.0 0 497 182 374 846.0 0 374 183 294 846.0 0 294 184 202 846.0 0 202 185 359 846.0 0 359 186 340 846.0 0 340 187 334 846.0 0 334 188 241 846.0 0 241 189 191 846.0 0 191 190 364 846.0 0 364 191 442 846.0 0 442 192 502 846.0 0 502 193 330 846.0 0 330 194 407 846.0 0 407 195 150 846.0 0 150 196 171 846.0 0 171 197 195 846.0 0 195 198 263 846.0 0 263 199 308 846.0 0 308 200 233 846.0 0 233 201 305 846.0 0 305 202 439 846.0 0 439 203 581 846.0 0 581 204 392 846.0 0 392 205 192 846.0 0 192 206 298 846.0 0 298 207 580 846.0 0 580 208 454 846.0 0 454 209 352 846.0 0 352 210 194 846.0 0 194 211 159 846.0 0 159 212 193 846.0 0 193 213 214 846.0 0 214 214 358 846.0 0 358 215 391 846.0 0 391 216 413 846.0 0 413 217 287 846.0 0 287 218 276 846.0 0 276 219 314 846.0 0 314 220 481 846.0 0 481 221 447 846.0 0 447 222 260 846.0 0 260 223 222 846.0 0 222 224 519 846.0 0 519 225 365 846.0 0 365 226 642 846.0 0 642 227 271 846.0 0 271 228 319 846.0 0 319 229 253 846.0 0 253 230 276 846.0 0 276 231 289 846.0 0 289 232 310 846.0 0 310 233 497 846.0 0 497 234 560 846.0 0 560 235 428 846.0 0 428 236 434 846.0 0 434 237 288 846.0 0 288 238 235 846.0 0 235 239 209 846.0 0 209 240 418 846.0 0 418 241 404 846.0 0 404 242 536 846.0 0 536 243 522 846.0 0 522 244 451 846.0 0 451 245 279 846.0 0 279 246 218 846.0 0 218 247 289 846.0 0 289 248 150 846.0 0 150 249 163 846.0 0 163 250 79 846.0 0 79 251 426 846.0 0 426 252 672 846.0 0 672 253 698 846.0 0 698 254 343 846.0 0 343 255 322 846.0 0 322 256 310 846.0 0 310 257 324 846.0 0 324 258 294 846.0 0 294 259 231 846.0 0 231 260 292 846.0 0 292 261 468 846.0 0 468 262 573 846.0 0 573 263 514 846.0 0 514 264 370 846.0 0 370 265 298 846.0 0 298 266 276 846.0 0 276 267 856 846.0 0 856 268 691 846.0 0 691 269 464 846.0 0 464 270 234 846.0 0 234 271 313 846.0 0 313 272 381 846.0 0 381 273 248 846.0 0 248 274 360 846.0 0 360 275 365 846.0 0 365 276 404 846.0 0 404 277 739 846.0 0 739 278 810 846.0 0 810 279 885 846.0 0 885 280 867 846.0 0 867 281 896 846.0 0 896 282 1205 846.0 0 1205 283 611 846.0 0 611 284 488 846.0 0 488 285 324 846.0 0 324 286 253 846.0 0 253 287 343 846.0 0 343 288 1653 846.0 0 1653 289 481 846.0 0 481 290 266 846.0 0 266 291 300 846.0 0 300 292 388 846.0 0 388 293 431 846.0 0 431 294 619 846.0 0 619 295 358 846.0 0 358 296 263 846.0 0 263 297 215 846.0 0 215 298 151 846.0 0 151 299 205 846.0 0 205 300 256 846.0 0 256 301 203 846.0 0 203 Finished in 6.74 s (40 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 166,428 Reads with adapters: 8,060 (4.8%) Reads that were too short: 152 (0.1%) Reads written (passing filters): 7,908 (4.8%) Total basepairs processed: 41,718,981 bp Total written (filtered): 2,147,583 bp (5.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8060 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 27 0.0 3 0 25 2 17 7501 0.0 3 7427 73 1 18 22 0.0 3 2 20 19 2 0.0 3 0 0 2 20 78 0.0 3 66 0 0 12 22 2 0.0 3 0 0 1 1 25 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 1 35 1 0.0 3 0 0 0 1 37 3 0.0 3 2 0 0 1 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 52 1 0.0 3 1 53 5 0.0 3 0 0 0 5 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 15 0.0 3 0 0 0 15 70 8 0.0 3 0 0 0 8 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 1 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 6 0.0 3 0 0 0 6 141 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 152 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 154 13 0.0 3 0 0 0 13 155 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 158 2 0.0 3 0 0 0 2 159 23 0.0 3 0 0 0 23 160 11 0.0 3 0 0 0 11 161 4 0.0 3 0 0 0 4 162 10 0.0 3 0 0 0 10 163 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 5 0.0 3 0 0 0 5 179 57 0.0 3 0 0 0 57 180 69 0.0 3 0 0 0 69 181 2 0.0 3 0 0 0 2 182 14 0.0 3 0 0 0 14 183 3 0.0 3 0 0 0 3 187 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 266 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 46 0.0 3 0 0 0 46 272 8 0.0 3 0 0 0 8 273 2 0.0 3 0 0 0 2 274 21 0.0 3 0 0 1 20 275 4 0.0 3 0 0 0 4 276 4 0.0 3 0 0 0 4 279 1 0.0 3 0 0 0 1 280 7 0.0 3 0 0 0 7 281 17 0.0 3 0 0 2 15 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 7,908 Reads with adapters: 661 (8.4%) Reads written (passing filters): 7,908 (100.0%) Total basepairs processed: 2,147,583 bp Total written (filtered): 2,145,600 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 661 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.4% C: 90.0% G: 5.1% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 661 123.6 0 661 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.