This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.69 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 378,810 (10.9%) Reads that were too short: 13,594 (0.4%) Reads written (passing filters): 365,216 (10.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 70,507,426 bp (6.8%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 378810 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 195 846.0 0 195 7 217 846.0 0 217 8 283 846.0 0 283 9 26044 846.0 0 26044 10 117 846.0 0 117 11 32 846.0 0 32 12 34 846.0 0 34 13 35 846.0 0 35 14 45 846.0 0 45 15 50 846.0 0 50 16 31 846.0 0 31 17 41 846.0 0 41 18 50 846.0 0 50 19 47 846.0 0 47 20 31 846.0 0 31 21 41 846.0 0 41 22 43 846.0 0 43 23 49 846.0 0 49 24 49 846.0 0 49 25 125 846.0 0 125 26 319 846.0 0 319 27 71 846.0 0 71 28 36 846.0 0 36 29 53 846.0 0 53 30 50 846.0 0 50 31 39 846.0 0 39 32 58 846.0 0 58 33 54 846.0 0 54 34 42 846.0 0 42 35 50 846.0 0 50 36 35 846.0 0 35 37 50 846.0 0 50 38 120 846.0 0 120 39 49 846.0 0 49 40 44 846.0 0 44 41 42 846.0 0 42 42 40 846.0 0 40 43 47 846.0 0 47 44 29 846.0 0 29 45 37 846.0 0 37 46 46 846.0 0 46 47 47 846.0 0 47 48 29 846.0 0 29 49 38 846.0 0 38 50 28 846.0 0 28 51 25 846.0 0 25 52 26 846.0 0 26 53 36 846.0 0 36 54 25 846.0 0 25 55 85 846.0 0 85 56 145 846.0 0 145 57 37 846.0 0 37 58 65 846.0 0 65 59 27 846.0 0 27 60 48 846.0 0 48 61 49 846.0 0 49 62 28 846.0 0 28 63 45 846.0 0 45 64 32 846.0 0 32 65 33 846.0 0 33 66 46 846.0 0 46 67 38 846.0 0 38 68 38 846.0 0 38 69 58 846.0 0 58 70 571 846.0 0 571 71 202 846.0 0 202 72 294 846.0 0 294 73 44 846.0 0 44 74 40 846.0 0 40 75 41 846.0 0 41 76 71 846.0 0 71 77 108 846.0 0 108 78 64 846.0 0 64 79 52 846.0 0 52 80 100 846.0 0 100 81 100 846.0 0 100 82 378 846.0 0 378 83 331 846.0 0 331 84 472 846.0 0 472 85 105 846.0 0 105 86 400 846.0 0 400 87 81 846.0 0 81 88 75 846.0 0 75 89 114 846.0 0 114 90 88 846.0 0 88 91 172 846.0 0 172 92 196 846.0 0 196 93 165 846.0 0 165 94 129 846.0 0 129 95 263 846.0 0 263 96 598 846.0 0 598 97 186 846.0 0 186 98 262 846.0 0 262 99 141 846.0 0 141 100 215 846.0 0 215 101 237 846.0 0 237 102 194 846.0 0 194 103 212 846.0 0 212 104 88 846.0 0 88 105 184 846.0 0 184 106 341 846.0 0 341 107 426 846.0 0 426 108 961 846.0 0 961 109 1006 846.0 0 1006 110 1481 846.0 0 1481 111 2727 846.0 0 2727 112 3703 846.0 0 3703 113 130516 846.0 0 130516 114 116244 846.0 0 116244 115 29109 846.0 0 29109 116 901 846.0 0 901 117 360 846.0 0 360 118 344 846.0 0 344 119 99 846.0 0 99 120 118 846.0 0 118 121 223 846.0 0 223 122 422 846.0 0 422 123 134 846.0 0 134 124 152 846.0 0 152 125 258 846.0 0 258 126 163 846.0 0 163 127 165 846.0 0 165 128 274 846.0 0 274 129 274 846.0 0 274 130 251 846.0 0 251 131 237 846.0 0 237 132 300 846.0 0 300 133 318 846.0 0 318 134 296 846.0 0 296 135 1623 846.0 0 1623 136 7821 846.0 0 7821 137 17954 846.0 0 17954 138 262 846.0 0 262 139 152 846.0 0 152 140 222 846.0 0 222 141 189 846.0 0 189 142 90 846.0 0 90 143 96 846.0 0 96 144 152 846.0 0 152 145 122 846.0 0 122 146 143 846.0 0 143 147 186 846.0 0 186 148 118 846.0 0 118 149 114 846.0 0 114 150 95 846.0 0 95 151 98 846.0 0 98 152 151 846.0 0 151 153 116 846.0 0 116 154 141 846.0 0 141 155 148 846.0 0 148 156 135 846.0 0 135 157 150 846.0 0 150 158 328 846.0 0 328 159 446 846.0 0 446 160 365 846.0 0 365 161 119 846.0 0 119 162 83 846.0 0 83 163 107 846.0 0 107 164 142 846.0 0 142 165 112 846.0 0 112 166 132 846.0 0 132 167 122 846.0 0 122 168 163 846.0 0 163 169 119 846.0 0 119 170 117 846.0 0 117 171 99 846.0 0 99 172 86 846.0 0 86 173 99 846.0 0 99 174 81 846.0 0 81 175 81 846.0 0 81 176 95 846.0 0 95 177 186 846.0 0 186 178 142 846.0 0 142 179 168 846.0 0 168 180 198 846.0 0 198 181 187 846.0 0 187 182 479 846.0 0 479 183 102 846.0 0 102 184 149 846.0 0 149 185 142 846.0 0 142 186 94 846.0 0 94 187 75 846.0 0 75 188 112 846.0 0 112 189 112 846.0 0 112 190 134 846.0 0 134 191 118 846.0 0 118 192 76 846.0 0 76 193 89 846.0 0 89 194 63 846.0 0 63 195 95 846.0 0 95 196 108 846.0 0 108 197 161 846.0 0 161 198 72 846.0 0 72 199 71 846.0 0 71 200 108 846.0 0 108 201 127 846.0 0 127 202 174 846.0 0 174 203 115 846.0 0 115 204 151 846.0 0 151 205 186 846.0 0 186 206 200 846.0 0 200 207 138 846.0 0 138 208 174 846.0 0 174 209 143 846.0 0 143 210 140 846.0 0 140 211 150 846.0 0 150 212 157 846.0 0 157 213 137 846.0 0 137 214 192 846.0 0 192 215 161 846.0 0 161 216 187 846.0 0 187 217 138 846.0 0 138 218 109 846.0 0 109 219 90 846.0 0 90 220 126 846.0 0 126 221 138 846.0 0 138 222 133 846.0 0 133 223 275 846.0 0 275 224 646 846.0 0 646 225 401 846.0 0 401 226 237 846.0 0 237 227 121 846.0 0 121 228 96 846.0 0 96 229 100 846.0 0 100 230 85 846.0 0 85 231 94 846.0 0 94 232 98 846.0 0 98 233 173 846.0 0 173 234 206 846.0 0 206 235 169 846.0 0 169 236 106 846.0 0 106 237 93 846.0 0 93 238 75 846.0 0 75 239 121 846.0 0 121 240 92 846.0 0 92 241 139 846.0 0 139 242 161 846.0 0 161 243 205 846.0 0 205 244 187 846.0 0 187 245 182 846.0 0 182 246 125 846.0 0 125 247 53 846.0 0 53 248 44 846.0 0 44 249 63 846.0 0 63 250 96 846.0 0 96 251 115 846.0 0 115 252 237 846.0 0 237 253 147 846.0 0 147 254 232 846.0 0 232 255 201 846.0 0 201 256 126 846.0 0 126 257 99 846.0 0 99 258 49 846.0 0 49 259 55 846.0 0 55 260 104 846.0 0 104 261 90 846.0 0 90 262 109 846.0 0 109 263 99 846.0 0 99 264 136 846.0 0 136 265 152 846.0 0 152 266 114 846.0 0 114 267 61 846.0 0 61 268 56 846.0 0 56 269 82 846.0 0 82 270 172 846.0 0 172 271 156 846.0 0 156 272 378 846.0 0 378 273 173 846.0 0 173 274 125 846.0 0 125 275 115 846.0 0 115 276 72 846.0 0 72 277 116 846.0 0 116 278 132 846.0 0 132 279 89 846.0 0 89 280 105 846.0 0 105 281 118 846.0 0 118 282 177 846.0 0 177 283 163 846.0 0 163 284 182 846.0 0 182 285 94 846.0 0 94 286 60 846.0 0 60 287 59 846.0 0 59 288 46 846.0 0 46 289 83 846.0 0 83 290 86 846.0 0 86 291 188 846.0 0 188 292 93 846.0 0 93 293 101 846.0 0 101 294 51 846.0 0 51 295 201 846.0 0 201 296 88 846.0 0 88 297 100 846.0 0 100 298 99 846.0 0 99 299 57 846.0 0 57 300 51 846.0 0 51 301 18 846.0 0 18 Finished in 14.31 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 365,216 Reads with adapters: 26,619 (7.3%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 26,606 (7.3%) Total basepairs processed: 70,507,426 bp Total written (filtered): 7,236,621 bp (10.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26619 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 4 0.0 3 0 0 3 1 19 66 0.0 3 0 54 5 5 2 20 26418 0.0 4 25105 1115 147 39 12 21 58 0.0 4 14 41 3 22 11 0.0 4 2 0 6 1 2 23 18 0.0 4 0 0 0 15 3 24 21 0.0 4 0 0 0 0 21 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 38 3 0.0 4 2 0 0 0 1 39 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 1 83 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 26,606 Reads with adapters: 8,110 (30.5%) Reads written (passing filters): 26,606 (100.0%) Total basepairs processed: 7,236,621 bp Total written (filtered): 5,436,278 bp (75.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8110 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.3% C: 0.7% G: 90.3% T: 3.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 415.7 0 4 4 2 103.9 0 2 8 3 0.4 0 3 9 427 0.1 0 38 389 10 499 0.0 1 34 465 11 318 0.0 1 3 315 12 1 0.0 1 0 1 13 2 0.0 1 0 2 150 6 0.0 1 5 1 172 1 0.0 1 1 189 1 0.0 1 0 1 208 6 0.0 1 5 1 210 1 0.0 1 0 1 229 1 0.0 1 1 243 1 0.0 1 1 254 2 0.0 1 2 256 3 0.0 1 3 257 322 0.0 1 311 11 258 4 0.0 1 4 259 6 0.0 1 6 260 22 0.0 1 20 2 261 6066 0.0 1 5801 265 262 63 0.0 1 59 4 263 39 0.0 1 35 4 264 34 0.0 1 33 1 266 267 0.0 1 259 8 267 3 0.0 1 3 268 1 0.0 1 1 269 4 0.0 1 4 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.