This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 87.66 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 152,325 (4.4%) Reads that were too short: 66,536 (1.9%) Reads written (passing filters): 85,789 (2.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 22,594,491 bp (2.2%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 152325 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 333 846.0 0 333 7 630 846.0 0 630 8 27042 846.0 0 27042 9 31787 846.0 0 31787 10 71 846.0 0 71 11 14 846.0 0 14 12 9 846.0 0 9 13 6 846.0 0 6 14 7 846.0 0 7 15 10 846.0 0 10 16 7 846.0 0 7 17 8 846.0 0 8 18 19 846.0 0 19 19 59 846.0 0 59 20 21 846.0 0 21 21 519 846.0 0 519 22 69 846.0 0 69 23 26 846.0 0 26 24 8 846.0 0 8 25 10 846.0 0 10 26 9 846.0 0 9 27 21 846.0 0 21 28 20 846.0 0 20 29 15 846.0 0 15 30 21 846.0 0 21 31 5 846.0 0 5 32 23 846.0 0 23 33 39 846.0 0 39 34 12 846.0 0 12 35 11 846.0 0 11 36 106 846.0 0 106 37 6 846.0 0 6 38 19 846.0 0 19 39 9 846.0 0 9 40 4 846.0 0 4 41 7 846.0 0 7 42 7 846.0 0 7 43 5 846.0 0 5 44 13 846.0 0 13 45 10 846.0 0 10 46 9 846.0 0 9 47 6 846.0 0 6 48 13 846.0 0 13 49 24 846.0 0 24 50 9 846.0 0 9 51 8 846.0 0 8 52 11 846.0 0 11 53 10 846.0 0 10 54 6 846.0 0 6 55 17 846.0 0 17 56 34 846.0 0 34 57 100 846.0 0 100 58 4887 846.0 0 4887 59 161 846.0 0 161 60 10 846.0 0 10 61 24 846.0 0 24 62 17 846.0 0 17 63 40 846.0 0 40 64 43 846.0 0 43 65 36 846.0 0 36 66 13 846.0 0 13 67 12 846.0 0 12 68 22 846.0 0 22 69 63 846.0 0 63 70 68 846.0 0 68 71 40 846.0 0 40 72 15 846.0 0 15 73 28 846.0 0 28 74 38 846.0 0 38 75 73 846.0 0 73 76 80 846.0 0 80 77 26 846.0 0 26 78 30 846.0 0 30 79 36 846.0 0 36 80 71 846.0 0 71 81 44 846.0 0 44 82 46 846.0 0 46 83 49 846.0 0 49 84 51 846.0 0 51 85 66 846.0 0 66 86 65 846.0 0 65 87 92 846.0 0 92 88 219 846.0 0 219 89 6094 846.0 0 6094 90 1204 846.0 0 1204 91 913 846.0 0 913 92 12 846.0 0 12 93 19 846.0 0 19 94 39 846.0 0 39 95 32 846.0 0 32 96 65 846.0 0 65 97 73 846.0 0 73 98 164 846.0 0 164 99 55 846.0 0 55 100 62 846.0 0 62 101 94 846.0 0 94 102 97 846.0 0 97 103 61 846.0 0 61 104 25 846.0 0 25 105 24 846.0 0 24 106 9 846.0 0 9 107 13 846.0 0 13 108 14 846.0 0 14 109 19 846.0 0 19 110 20 846.0 0 20 111 49 846.0 0 49 112 54 846.0 0 54 113 84 846.0 0 84 114 218 846.0 0 218 115 46 846.0 0 46 116 45 846.0 0 45 117 15 846.0 0 15 118 16 846.0 0 16 119 11 846.0 0 11 120 24 846.0 0 24 121 39 846.0 0 39 122 52 846.0 0 52 123 22 846.0 0 22 124 42 846.0 0 42 125 101 846.0 0 101 126 60 846.0 0 60 127 37 846.0 0 37 128 37 846.0 0 37 129 34 846.0 0 34 130 30 846.0 0 30 131 35 846.0 0 35 132 52 846.0 0 52 133 168 846.0 0 168 134 55 846.0 0 55 135 45 846.0 0 45 136 22 846.0 0 22 137 30 846.0 0 30 138 33 846.0 0 33 139 86 846.0 0 86 140 49 846.0 0 49 141 58 846.0 0 58 142 38 846.0 0 38 143 26 846.0 0 26 144 50 846.0 0 50 145 84 846.0 0 84 146 83 846.0 0 83 147 75 846.0 0 75 148 384 846.0 0 384 149 260 846.0 0 260 150 294 846.0 0 294 151 28 846.0 0 28 152 34 846.0 0 34 153 50 846.0 0 50 154 36 846.0 0 36 155 27 846.0 0 27 156 38 846.0 0 38 157 64 846.0 0 64 158 52 846.0 0 52 159 64 846.0 0 64 160 46 846.0 0 46 161 53 846.0 0 53 162 55 846.0 0 55 163 27 846.0 0 27 164 45 846.0 0 45 165 43 846.0 0 43 166 38 846.0 0 38 167 43 846.0 0 43 168 557 846.0 0 557 169 217 846.0 0 217 170 421 846.0 0 421 171 23 846.0 0 23 172 35 846.0 0 35 173 40 846.0 0 40 174 63 846.0 0 63 175 59 846.0 0 59 176 1133 846.0 0 1133 177 1252 846.0 0 1252 178 506 846.0 0 506 179 44 846.0 0 44 180 43 846.0 0 43 181 23 846.0 0 23 182 56 846.0 0 56 183 40 846.0 0 40 184 44 846.0 0 44 185 53 846.0 0 53 186 57 846.0 0 57 187 41 846.0 0 41 188 22 846.0 0 22 189 35 846.0 0 35 190 33 846.0 0 33 191 44 846.0 0 44 192 34 846.0 0 34 193 63 846.0 0 63 194 69 846.0 0 69 195 48 846.0 0 48 196 42 846.0 0 42 197 31 846.0 0 31 198 35 846.0 0 35 199 30 846.0 0 30 200 15 846.0 0 15 201 58 846.0 0 58 202 57 846.0 0 57 203 71 846.0 0 71 204 66 846.0 0 66 205 78 846.0 0 78 206 68 846.0 0 68 207 273 846.0 0 273 208 484 846.0 0 484 209 200 846.0 0 200 210 83 846.0 0 83 211 60 846.0 0 60 212 53 846.0 0 53 213 62 846.0 0 62 214 188 846.0 0 188 215 120 846.0 0 120 216 127 846.0 0 127 217 102 846.0 0 102 218 144 846.0 0 144 219 308 846.0 0 308 220 4086 846.0 0 4086 221 3635 846.0 0 3635 222 2317 846.0 0 2317 223 59 846.0 0 59 224 59 846.0 0 59 225 71 846.0 0 71 226 76 846.0 0 76 227 60 846.0 0 60 228 68 846.0 0 68 229 49 846.0 0 49 230 96 846.0 0 96 231 106 846.0 0 106 232 84 846.0 0 84 233 57 846.0 0 57 234 79 846.0 0 79 235 55 846.0 0 55 236 52 846.0 0 52 237 84 846.0 0 84 238 127 846.0 0 127 239 241 846.0 0 241 240 214 846.0 0 214 241 302 846.0 0 302 242 134 846.0 0 134 243 92 846.0 0 92 244 124 846.0 0 124 245 88 846.0 0 88 246 191 846.0 0 191 247 75 846.0 0 75 248 64 846.0 0 64 249 40 846.0 0 40 250 36 846.0 0 36 251 44 846.0 0 44 252 35 846.0 0 35 253 76 846.0 0 76 254 99 846.0 0 99 255 163 846.0 0 163 256 277 846.0 0 277 257 367 846.0 0 367 258 112 846.0 0 112 259 73 846.0 0 73 260 78 846.0 0 78 261 78 846.0 0 78 262 59 846.0 0 59 263 79 846.0 0 79 264 88 846.0 0 88 265 113 846.0 0 113 266 188 846.0 0 188 267 85 846.0 0 85 268 127 846.0 0 127 269 449 846.0 0 449 270 316 846.0 0 316 271 268 846.0 0 268 272 135 846.0 0 135 273 136 846.0 0 136 274 519 846.0 0 519 275 685 846.0 0 685 276 1206 846.0 0 1206 277 91 846.0 0 91 278 107 846.0 0 107 279 196 846.0 0 196 280 310 846.0 0 310 281 751 846.0 0 751 282 928 846.0 0 928 283 19654 846.0 0 19654 284 11903 846.0 0 11903 285 9142 846.0 0 9142 286 531 846.0 0 531 287 81 846.0 0 81 288 419 846.0 0 419 289 69 846.0 0 69 290 92 846.0 0 92 291 58 846.0 0 58 292 100 846.0 0 100 293 170 846.0 0 170 294 167 846.0 0 167 295 83 846.0 0 83 296 255 846.0 0 255 297 27 846.0 0 27 298 30 846.0 0 30 299 30 846.0 0 30 300 16 846.0 0 16 301 106 846.0 0 106 Finished in 3.84 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 85,789 Reads with adapters: 27,432 (32.0%) Reads that were too short: 67 (0.1%) Reads written (passing filters): 27,365 (31.9%) Total basepairs processed: 22,594,491 bp Total written (filtered): 7,550,021 bp (33.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 27432 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 92 0.0 3 0 91 1 17 27154 0.0 3 26866 284 4 18 64 0.0 3 2 59 3 19 7 0.0 3 0 1 5 1 20 17 0.0 3 0 0 0 17 32 1 0.0 3 1 39 1 0.0 3 0 0 0 1 43 6 0.0 3 0 0 0 6 46 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 1 97 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 217 57 0.0 3 0 0 0 57 227 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.99 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 27,365 Reads with adapters: 720 (2.6%) Reads written (passing filters): 27,365 (100.0%) Total basepairs processed: 7,550,021 bp Total written (filtered): 7,547,809 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 720 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.1% C: 24.6% G: 19.4% T: 7.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 691 427.6 0 691 4 19 106.9 0 19 5 4 26.7 0 4 6 3 6.7 0 3 7 1 1.7 0 1 8 1 0.4 0 1 10 1 0.0 1 0 1