This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 88.33 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 53,809 (1.6%) Reads that were too short: 4,961 (0.1%) Reads written (passing filters): 48,848 (1.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 11,807,122 bp (1.1%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 53809 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 356 846.0 0 356 7 1458 846.0 0 1458 8 5284 846.0 0 5284 9 48 846.0 0 48 10 31 846.0 0 31 11 12 846.0 0 12 12 25 846.0 0 25 13 42 846.0 0 42 14 13 846.0 0 13 15 20 846.0 0 20 16 6 846.0 0 6 17 18 846.0 0 18 18 26 846.0 0 26 19 19 846.0 0 19 20 16 846.0 0 16 21 25 846.0 0 25 22 25 846.0 0 25 23 23 846.0 0 23 24 19 846.0 0 19 25 16 846.0 0 16 26 7 846.0 0 7 27 13 846.0 0 13 28 45 846.0 0 45 29 19 846.0 0 19 30 15 846.0 0 15 31 17 846.0 0 17 32 16 846.0 0 16 33 16 846.0 0 16 34 25 846.0 0 25 35 28 846.0 0 28 36 39 846.0 0 39 37 50 846.0 0 50 38 363 846.0 0 363 39 660 846.0 0 660 40 69 846.0 0 69 41 413 846.0 0 413 42 44 846.0 0 44 43 158 846.0 0 158 44 64 846.0 0 64 45 45 846.0 0 45 46 114 846.0 0 114 47 298 846.0 0 298 48 80 846.0 0 80 49 389 846.0 0 389 50 120 846.0 0 120 51 151 846.0 0 151 52 189 846.0 0 189 53 427 846.0 0 427 54 575 846.0 0 575 55 12893 846.0 0 12893 56 6514 846.0 0 6514 57 7669 846.0 0 7669 58 69 846.0 0 69 59 14 846.0 0 14 60 14 846.0 0 14 61 12 846.0 0 12 62 12 846.0 0 12 63 18 846.0 0 18 64 12 846.0 0 12 65 16 846.0 0 16 66 15 846.0 0 15 67 19 846.0 0 19 68 28 846.0 0 28 69 11 846.0 0 11 70 23 846.0 0 23 71 16 846.0 0 16 72 23 846.0 0 23 73 34 846.0 0 34 74 28 846.0 0 28 75 27 846.0 0 27 76 29 846.0 0 29 77 28 846.0 0 28 78 20 846.0 0 20 79 20 846.0 0 20 80 32 846.0 0 32 81 48 846.0 0 48 82 56 846.0 0 56 83 66 846.0 0 66 84 74 846.0 0 74 85 1978 846.0 0 1978 86 787 846.0 0 787 87 1588 846.0 0 1588 88 51 846.0 0 51 89 81 846.0 0 81 90 25 846.0 0 25 91 21 846.0 0 21 92 27 846.0 0 27 93 33 846.0 0 33 94 54 846.0 0 54 95 20 846.0 0 20 96 25 846.0 0 25 97 23 846.0 0 23 98 12 846.0 0 12 99 15 846.0 0 15 100 21 846.0 0 21 101 27 846.0 0 27 102 90 846.0 0 90 103 94 846.0 0 94 104 32 846.0 0 32 105 24 846.0 0 24 106 13 846.0 0 13 107 11 846.0 0 11 108 13 846.0 0 13 109 16 846.0 0 16 110 23 846.0 0 23 111 37 846.0 0 37 112 22 846.0 0 22 113 18 846.0 0 18 114 13 846.0 0 13 115 20 846.0 0 20 116 16 846.0 0 16 117 20 846.0 0 20 118 68 846.0 0 68 119 48 846.0 0 48 120 21 846.0 0 21 121 20 846.0 0 20 122 21 846.0 0 21 123 40 846.0 0 40 124 43 846.0 0 43 125 32 846.0 0 32 126 127 846.0 0 127 127 114 846.0 0 114 128 98 846.0 0 98 129 24 846.0 0 24 130 37 846.0 0 37 131 76 846.0 0 76 132 46 846.0 0 46 133 60 846.0 0 60 134 33 846.0 0 33 135 29 846.0 0 29 136 27 846.0 0 27 137 125 846.0 0 125 138 96 846.0 0 96 139 135 846.0 0 135 140 46 846.0 0 46 141 25 846.0 0 25 142 28 846.0 0 28 143 22 846.0 0 22 144 21 846.0 0 21 145 23 846.0 0 23 146 47 846.0 0 47 147 13 846.0 0 13 148 33 846.0 0 33 149 28 846.0 0 28 150 24 846.0 0 24 151 37 846.0 0 37 152 23 846.0 0 23 153 32 846.0 0 32 154 37 846.0 0 37 155 16 846.0 0 16 156 29 846.0 0 29 157 119 846.0 0 119 158 70 846.0 0 70 159 89 846.0 0 89 160 35 846.0 0 35 161 31 846.0 0 31 162 101 846.0 0 101 163 29 846.0 0 29 164 43 846.0 0 43 165 34 846.0 0 34 166 36 846.0 0 36 167 76 846.0 0 76 168 41 846.0 0 41 169 37 846.0 0 37 170 32 846.0 0 32 171 30 846.0 0 30 172 39 846.0 0 39 173 18 846.0 0 18 174 19 846.0 0 19 175 25 846.0 0 25 176 27 846.0 0 27 177 24 846.0 0 24 178 17 846.0 0 17 179 22 846.0 0 22 180 31 846.0 0 31 181 40 846.0 0 40 182 29 846.0 0 29 183 46 846.0 0 46 184 36 846.0 0 36 185 33 846.0 0 33 186 23 846.0 0 23 187 128 846.0 0 128 188 135 846.0 0 135 189 75 846.0 0 75 190 84 846.0 0 84 191 66 846.0 0 66 192 27 846.0 0 27 193 24 846.0 0 24 194 27 846.0 0 27 195 28 846.0 0 28 196 18 846.0 0 18 197 18 846.0 0 18 198 21 846.0 0 21 199 40 846.0 0 40 200 75 846.0 0 75 201 25 846.0 0 25 202 20 846.0 0 20 203 17 846.0 0 17 204 24 846.0 0 24 205 39 846.0 0 39 206 56 846.0 0 56 207 56 846.0 0 56 208 35 846.0 0 35 209 26 846.0 0 26 210 19 846.0 0 19 211 21 846.0 0 21 212 33 846.0 0 33 213 64 846.0 0 64 214 39 846.0 0 39 215 46 846.0 0 46 216 42 846.0 0 42 217 41 846.0 0 41 218 55 846.0 0 55 219 274 846.0 0 274 220 1087 846.0 0 1087 221 71 846.0 0 71 222 30 846.0 0 30 223 29 846.0 0 29 224 27 846.0 0 27 225 47 846.0 0 47 226 45 846.0 0 45 227 23 846.0 0 23 228 28 846.0 0 28 229 33 846.0 0 33 230 17 846.0 0 17 231 21 846.0 0 21 232 16 846.0 0 16 233 25 846.0 0 25 234 22 846.0 0 22 235 33 846.0 0 33 236 34 846.0 0 34 237 9 846.0 0 9 238 37 846.0 0 37 239 35 846.0 0 35 240 53 846.0 0 53 241 27 846.0 0 27 242 26 846.0 0 26 243 25 846.0 0 25 244 36 846.0 0 36 245 41 846.0 0 41 246 38 846.0 0 38 247 59 846.0 0 59 248 12 846.0 0 12 249 21 846.0 0 21 250 17 846.0 0 17 251 31 846.0 0 31 252 19 846.0 0 19 253 34 846.0 0 34 254 23 846.0 0 23 255 32 846.0 0 32 256 28 846.0 0 28 257 106 846.0 0 106 258 27 846.0 0 27 259 28 846.0 0 28 260 16 846.0 0 16 261 27 846.0 0 27 262 46 846.0 0 46 263 10 846.0 0 10 264 22 846.0 0 22 265 39 846.0 0 39 266 34 846.0 0 34 267 29 846.0 0 29 268 21 846.0 0 21 269 25 846.0 0 25 270 14 846.0 0 14 271 11 846.0 0 11 272 22 846.0 0 22 273 19 846.0 0 19 274 23 846.0 0 23 275 34 846.0 0 34 276 148 846.0 0 148 277 37 846.0 0 37 278 311 846.0 0 311 279 28 846.0 0 28 280 40 846.0 0 40 281 60 846.0 0 60 282 30 846.0 0 30 283 40 846.0 0 40 284 15 846.0 0 15 285 30 846.0 0 30 286 17 846.0 0 17 287 48 846.0 0 48 288 25 846.0 0 25 289 34 846.0 0 34 290 13 846.0 0 13 291 22 846.0 0 22 292 27 846.0 0 27 293 16 846.0 0 16 294 35 846.0 0 35 295 27 846.0 0 27 296 78 846.0 0 78 297 60 846.0 0 60 298 42 846.0 0 42 299 60 846.0 0 60 300 56 846.0 0 56 301 11 846.0 0 11 Finished in 2.27 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 48,848 Reads with adapters: 5,702 (11.7%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 5,696 (11.7%) Total basepairs processed: 11,807,122 bp Total written (filtered): 1,551,376 bp (13.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5702 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 0 1 19 15 0.0 3 0 14 0 1 20 5364 0.0 4 5246 104 7 5 2 21 13 0.0 4 0 12 1 22 2 0.0 4 0 2 23 273 0.0 4 250 14 1 7 1 24 1 0.0 4 0 1 28 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 1 63 1 0.0 4 1 66 1 0.0 4 0 1 77 2 0.0 4 0 0 0 0 2 123 13 0.0 4 0 0 0 0 13 124 1 0.0 4 0 0 0 0 1 127 7 0.0 4 0 0 0 0 7 132 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 5,696 Reads with adapters: 417 (7.3%) Reads written (passing filters): 5,696 (100.0%) Total basepairs processed: 1,551,376 bp Total written (filtered): 1,544,545 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 417 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.8% C: 2.6% G: 92.1% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 2 0.0 0 0 2 10 22 0.0 1 1 21 11 383 0.0 1 2 381 12 1 0.0 1 1 224 1 0.0 1 1 262 2 0.0 1 2 264 1 0.0 1 1 271 4 0.0 1 3 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.