This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 90.92 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 300,225 (8.7%) Reads that were too short: 135,144 (3.9%) Reads written (passing filters): 165,081 (4.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 27,125,877 bp (2.6%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 300225 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 107 846.0 0 107 7 6802 846.0 0 6802 8 82 846.0 0 82 9 60 846.0 0 60 10 94 846.0 0 94 11 71 846.0 0 71 12 68 846.0 0 68 13 72 846.0 0 72 14 66 846.0 0 66 15 58 846.0 0 58 16 66 846.0 0 66 17 64 846.0 0 64 18 64 846.0 0 64 19 76 846.0 0 76 20 75 846.0 0 75 21 90 846.0 0 90 22 63 846.0 0 63 23 80 846.0 0 80 24 74 846.0 0 74 25 79 846.0 0 79 26 62 846.0 0 62 27 73 846.0 0 73 28 68 846.0 0 68 29 69 846.0 0 69 30 50 846.0 0 50 31 84 846.0 0 84 32 51 846.0 0 51 33 74 846.0 0 74 34 92 846.0 0 92 35 66 846.0 0 66 36 80 846.0 0 80 37 64 846.0 0 64 38 59 846.0 0 59 39 92 846.0 0 92 40 53 846.0 0 53 41 83 846.0 0 83 42 638 846.0 0 638 43 82 846.0 0 82 44 64 846.0 0 64 45 77 846.0 0 77 46 67 846.0 0 67 47 72 846.0 0 72 48 69 846.0 0 69 49 65 846.0 0 65 50 65 846.0 0 65 51 67 846.0 0 67 52 60 846.0 0 60 53 68 846.0 0 68 54 57 846.0 0 57 55 62 846.0 0 62 56 74 846.0 0 74 57 79 846.0 0 79 58 71 846.0 0 71 59 49 846.0 0 49 60 90 846.0 0 90 61 72 846.0 0 72 62 62 846.0 0 62 63 81 846.0 0 81 64 64 846.0 0 64 65 63 846.0 0 63 66 92 846.0 0 92 67 88 846.0 0 88 68 84 846.0 0 84 69 157 846.0 0 157 70 74 846.0 0 74 71 66 846.0 0 66 72 63 846.0 0 63 73 103 846.0 0 103 74 265 846.0 0 265 75 55 846.0 0 55 76 150 846.0 0 150 77 78 846.0 0 78 78 129 846.0 0 129 79 129 846.0 0 129 80 187 846.0 0 187 81 223 846.0 0 223 82 452 846.0 0 452 83 569 846.0 0 569 84 639 846.0 0 639 85 475 846.0 0 475 86 469 846.0 0 469 87 1724 846.0 0 1724 88 1033 846.0 0 1033 89 785 846.0 0 785 90 756 846.0 0 756 91 444 846.0 0 444 92 341 846.0 0 341 93 1674 846.0 0 1674 94 2092 846.0 0 2092 95 2061 846.0 0 2061 96 658 846.0 0 658 97 393 846.0 0 393 98 523 846.0 0 523 99 315 846.0 0 315 100 337 846.0 0 337 101 501 846.0 0 501 102 455 846.0 0 455 103 599 846.0 0 599 104 698 846.0 0 698 105 497 846.0 0 497 106 670 846.0 0 670 107 1550 846.0 0 1550 108 2052 846.0 0 2052 109 2668 846.0 0 2668 110 489 846.0 0 489 111 623 846.0 0 623 112 516 846.0 0 516 113 614 846.0 0 614 114 699 846.0 0 699 115 593 846.0 0 593 116 450 846.0 0 450 117 369 846.0 0 369 118 397 846.0 0 397 119 646 846.0 0 646 120 1243 846.0 0 1243 121 1051 846.0 0 1051 122 574 846.0 0 574 123 585 846.0 0 585 124 619 846.0 0 619 125 748 846.0 0 748 126 1012 846.0 0 1012 127 1102 846.0 0 1102 128 963 846.0 0 963 129 1280 846.0 0 1280 130 550 846.0 0 550 131 483 846.0 0 483 132 658 846.0 0 658 133 2030 846.0 0 2030 134 2970 846.0 0 2970 135 3521 846.0 0 3521 136 4998 846.0 0 4998 137 880 846.0 0 880 138 653 846.0 0 653 139 896 846.0 0 896 140 1126 846.0 0 1126 141 1409 846.0 0 1409 142 852 846.0 0 852 143 548 846.0 0 548 144 769 846.0 0 769 145 1275 846.0 0 1275 146 1351 846.0 0 1351 147 1283 846.0 0 1283 148 1373 846.0 0 1373 149 885 846.0 0 885 150 854 846.0 0 854 151 953 846.0 0 953 152 1558 846.0 0 1558 153 1762 846.0 0 1762 154 1549 846.0 0 1549 155 1936 846.0 0 1936 156 1965 846.0 0 1965 157 9315 846.0 0 9315 158 14616 846.0 0 14616 159 4657 846.0 0 4657 160 1418 846.0 0 1418 161 1296 846.0 0 1296 162 927 846.0 0 927 163 683 846.0 0 683 164 1024 846.0 0 1024 165 1634 846.0 0 1634 166 1500 846.0 0 1500 167 1005 846.0 0 1005 168 654 846.0 0 654 169 723 846.0 0 723 170 703 846.0 0 703 171 854 846.0 0 854 172 905 846.0 0 905 173 2013 846.0 0 2013 174 2590 846.0 0 2590 175 1415 846.0 0 1415 176 760 846.0 0 760 177 556 846.0 0 556 178 544 846.0 0 544 179 637 846.0 0 637 180 1147 846.0 0 1147 181 1521 846.0 0 1521 182 1227 846.0 0 1227 183 758 846.0 0 758 184 712 846.0 0 712 185 970 846.0 0 970 186 1869 846.0 0 1869 187 1160 846.0 0 1160 188 771 846.0 0 771 189 646 846.0 0 646 190 1060 846.0 0 1060 191 1509 846.0 0 1509 192 1344 846.0 0 1344 193 1224 846.0 0 1224 194 724 846.0 0 724 195 644 846.0 0 644 196 464 846.0 0 464 197 505 846.0 0 505 198 901 846.0 0 901 199 761 846.0 0 761 200 927 846.0 0 927 201 1226 846.0 0 1226 202 897 846.0 0 897 203 1580 846.0 0 1580 204 1024 846.0 0 1024 205 755 846.0 0 755 206 939 846.0 0 939 207 1014 846.0 0 1014 208 998 846.0 0 998 209 659 846.0 0 659 210 635 846.0 0 635 211 533 846.0 0 533 212 662 846.0 0 662 213 1008 846.0 0 1008 214 1153 846.0 0 1153 215 937 846.0 0 937 216 1111 846.0 0 1111 217 1044 846.0 0 1044 218 959 846.0 0 959 219 1032 846.0 0 1032 220 1307 846.0 0 1307 221 1450 846.0 0 1450 222 813 846.0 0 813 223 554 846.0 0 554 224 752 846.0 0 752 225 824 846.0 0 824 226 901 846.0 0 901 227 997 846.0 0 997 228 1044 846.0 0 1044 229 959 846.0 0 959 230 982 846.0 0 982 231 983 846.0 0 983 232 1051 846.0 0 1051 233 1230 846.0 0 1230 234 1022 846.0 0 1022 235 852 846.0 0 852 236 1132 846.0 0 1132 237 840 846.0 0 840 238 708 846.0 0 708 239 814 846.0 0 814 240 861 846.0 0 861 241 962 846.0 0 962 242 932 846.0 0 932 243 971 846.0 0 971 244 897 846.0 0 897 245 796 846.0 0 796 246 922 846.0 0 922 247 850 846.0 0 850 248 858 846.0 0 858 249 443 846.0 0 443 250 693 846.0 0 693 251 853 846.0 0 853 252 2029 846.0 0 2029 253 2823 846.0 0 2823 254 1272 846.0 0 1272 255 783 846.0 0 783 256 1049 846.0 0 1049 257 1211 846.0 0 1211 258 1030 846.0 0 1030 259 791 846.0 0 791 260 1034 846.0 0 1034 261 966 846.0 0 966 262 962 846.0 0 962 263 815 846.0 0 815 264 690 846.0 0 690 265 620 846.0 0 620 266 1374 846.0 0 1374 267 1329 846.0 0 1329 268 1687 846.0 0 1687 269 1566 846.0 0 1566 270 1001 846.0 0 1001 271 1026 846.0 0 1026 272 1156 846.0 0 1156 273 1010 846.0 0 1010 274 884 846.0 0 884 275 1098 846.0 0 1098 276 2752 846.0 0 2752 277 4886 846.0 0 4886 278 2276 846.0 0 2276 279 1839 846.0 0 1839 280 1651 846.0 0 1651 281 1917 846.0 0 1917 282 982 846.0 0 982 283 716 846.0 0 716 284 1291 846.0 0 1291 285 1209 846.0 0 1209 286 1129 846.0 0 1129 287 1141 846.0 0 1141 288 1306 846.0 0 1306 289 1044 846.0 0 1044 290 874 846.0 0 874 291 905 846.0 0 905 292 1213 846.0 0 1213 293 1505 846.0 0 1505 294 1974 846.0 0 1974 295 4312 846.0 0 4312 296 7313 846.0 0 7313 297 14596 846.0 0 14596 298 594 846.0 0 594 299 542 846.0 0 542 300 800 846.0 0 800 301 1978 846.0 0 1978 Finished in 5.12 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 165,081 Reads with adapters: 2,591 (1.6%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 2,557 (1.5%) Total basepairs processed: 27,125,877 bp Total written (filtered): 703,073 bp (2.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2591 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 6 0.0 3 0 6 17 2514 0.0 3 2086 401 27 19 4 0.0 3 0 0 2 2 20 1 0.0 3 0 0 0 1 21 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 4 0.0 3 0 0 0 4 33 1 0.0 3 0 0 0 1 37 5 0.0 3 0 0 0 5 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 58 6 0.0 3 0 0 0 6 61 4 0.0 3 0 0 0 4 67 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 2,557 Reads with adapters: 254 (9.9%) Reads written (passing filters): 2,557 (100.0%) Total basepairs processed: 703,073 bp Total written (filtered): 702,311 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 254 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 3.5% C: 93.7% G: 2.8% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 254 40.0 0 254 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.