This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 89.99 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 46,449 (1.3%) Reads that were too short: 9,483 (0.3%) Reads written (passing filters): 36,966 (1.1%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 9,455,928 bp (0.9%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 46449 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 134 846.0 0 134 7 560 846.0 0 560 8 8667 846.0 0 8667 9 15040 846.0 0 15040 10 104 846.0 0 104 11 7 846.0 0 7 12 7 846.0 0 7 13 11 846.0 0 11 14 12 846.0 0 12 15 10 846.0 0 10 16 6 846.0 0 6 17 7 846.0 0 7 18 4 846.0 0 4 19 7 846.0 0 7 20 12 846.0 0 12 21 8 846.0 0 8 22 15 846.0 0 15 23 16 846.0 0 16 24 6 846.0 0 6 25 5 846.0 0 5 26 2 846.0 0 2 27 11 846.0 0 11 28 6 846.0 0 6 29 18 846.0 0 18 30 13 846.0 0 13 31 11 846.0 0 11 32 6 846.0 0 6 33 77 846.0 0 77 34 12 846.0 0 12 35 23 846.0 0 23 36 183 846.0 0 183 37 11 846.0 0 11 38 39 846.0 0 39 39 13 846.0 0 13 40 9 846.0 0 9 41 9 846.0 0 9 42 8 846.0 0 8 43 6 846.0 0 6 44 13 846.0 0 13 45 83 846.0 0 83 46 19 846.0 0 19 47 7 846.0 0 7 48 7 846.0 0 7 49 12 846.0 0 12 50 20 846.0 0 20 51 10 846.0 0 10 52 7 846.0 0 7 53 13 846.0 0 13 54 10 846.0 0 10 55 7 846.0 0 7 56 10 846.0 0 10 57 18 846.0 0 18 58 124 846.0 0 124 59 68 846.0 0 68 60 155 846.0 0 155 61 10 846.0 0 10 62 31 846.0 0 31 63 23 846.0 0 23 64 9 846.0 0 9 65 10 846.0 0 10 66 10 846.0 0 10 67 21 846.0 0 21 68 11 846.0 0 11 69 13 846.0 0 13 70 24 846.0 0 24 71 9 846.0 0 9 72 11 846.0 0 11 73 18 846.0 0 18 74 17 846.0 0 17 75 62 846.0 0 62 76 1773 846.0 0 1773 77 46 846.0 0 46 78 245 846.0 0 245 79 20 846.0 0 20 80 327 846.0 0 327 81 415 846.0 0 415 82 83 846.0 0 83 83 27 846.0 0 27 84 19 846.0 0 19 85 19 846.0 0 19 86 15 846.0 0 15 87 65 846.0 0 65 88 28 846.0 0 28 89 65 846.0 0 65 90 43 846.0 0 43 91 39 846.0 0 39 92 15 846.0 0 15 93 39 846.0 0 39 94 42 846.0 0 42 95 66 846.0 0 66 96 46 846.0 0 46 97 62 846.0 0 62 98 98 846.0 0 98 99 46 846.0 0 46 100 321 846.0 0 321 101 804 846.0 0 804 102 275 846.0 0 275 103 25 846.0 0 25 104 7 846.0 0 7 105 33 846.0 0 33 106 19 846.0 0 19 107 9 846.0 0 9 108 18 846.0 0 18 109 71 846.0 0 71 110 30 846.0 0 30 111 56 846.0 0 56 112 257 846.0 0 257 113 84 846.0 0 84 114 20 846.0 0 20 115 21 846.0 0 21 116 29 846.0 0 29 117 33 846.0 0 33 118 31 846.0 0 31 119 33 846.0 0 33 120 22 846.0 0 22 121 24 846.0 0 24 122 28 846.0 0 28 123 35 846.0 0 35 124 29 846.0 0 29 125 26 846.0 0 26 126 25 846.0 0 25 127 53 846.0 0 53 128 25 846.0 0 25 129 27 846.0 0 27 130 43 846.0 0 43 131 46 846.0 0 46 132 61 846.0 0 61 133 59 846.0 0 59 134 54 846.0 0 54 135 29 846.0 0 29 136 38 846.0 0 38 137 37 846.0 0 37 138 76 846.0 0 76 139 39 846.0 0 39 140 31 846.0 0 31 141 50 846.0 0 50 142 48 846.0 0 48 143 49 846.0 0 49 144 48 846.0 0 48 145 40 846.0 0 40 146 30 846.0 0 30 147 148 846.0 0 148 148 41 846.0 0 41 149 64 846.0 0 64 150 55 846.0 0 55 151 55 846.0 0 55 152 25 846.0 0 25 153 35 846.0 0 35 154 40 846.0 0 40 155 66 846.0 0 66 156 64 846.0 0 64 157 64 846.0 0 64 158 79 846.0 0 79 159 62 846.0 0 62 160 85 846.0 0 85 161 62 846.0 0 62 162 121 846.0 0 121 163 62 846.0 0 62 164 55 846.0 0 55 165 42 846.0 0 42 166 41 846.0 0 41 167 62 846.0 0 62 168 162 846.0 0 162 169 139 846.0 0 139 170 179 846.0 0 179 171 41 846.0 0 41 172 39 846.0 0 39 173 34 846.0 0 34 174 42 846.0 0 42 175 50 846.0 0 50 176 60 846.0 0 60 177 96 846.0 0 96 178 72 846.0 0 72 179 56 846.0 0 56 180 49 846.0 0 49 181 50 846.0 0 50 182 60 846.0 0 60 183 66 846.0 0 66 184 84 846.0 0 84 185 63 846.0 0 63 186 78 846.0 0 78 187 99 846.0 0 99 188 71 846.0 0 71 189 67 846.0 0 67 190 25 846.0 0 25 191 56 846.0 0 56 192 68 846.0 0 68 193 54 846.0 0 54 194 73 846.0 0 73 195 137 846.0 0 137 196 69 846.0 0 69 197 100 846.0 0 100 198 144 846.0 0 144 199 109 846.0 0 109 200 193 846.0 0 193 201 105 846.0 0 105 202 97 846.0 0 97 203 58 846.0 0 58 204 239 846.0 0 239 205 116 846.0 0 116 206 100 846.0 0 100 207 109 846.0 0 109 208 89 846.0 0 89 209 88 846.0 0 88 210 62 846.0 0 62 211 73 846.0 0 73 212 101 846.0 0 101 213 114 846.0 0 114 214 111 846.0 0 111 215 93 846.0 0 93 216 114 846.0 0 114 217 123 846.0 0 123 218 119 846.0 0 119 219 117 846.0 0 117 220 108 846.0 0 108 221 121 846.0 0 121 222 203 846.0 0 203 223 181 846.0 0 181 224 339 846.0 0 339 225 114 846.0 0 114 226 87 846.0 0 87 227 82 846.0 0 82 228 274 846.0 0 274 229 96 846.0 0 96 230 77 846.0 0 77 231 58 846.0 0 58 232 67 846.0 0 67 233 64 846.0 0 64 234 73 846.0 0 73 235 62 846.0 0 62 236 83 846.0 0 83 237 187 846.0 0 187 238 82 846.0 0 82 239 85 846.0 0 85 240 214 846.0 0 214 241 196 846.0 0 196 242 179 846.0 0 179 243 93 846.0 0 93 244 91 846.0 0 91 245 95 846.0 0 95 246 53 846.0 0 53 247 70 846.0 0 70 248 82 846.0 0 82 249 89 846.0 0 89 250 61 846.0 0 61 251 49 846.0 0 49 252 28 846.0 0 28 253 73 846.0 0 73 254 100 846.0 0 100 255 122 846.0 0 122 256 120 846.0 0 120 257 96 846.0 0 96 258 105 846.0 0 105 259 98 846.0 0 98 260 82 846.0 0 82 261 40 846.0 0 40 262 73 846.0 0 73 263 54 846.0 0 54 264 68 846.0 0 68 265 67 846.0 0 67 266 64 846.0 0 64 267 62 846.0 0 62 268 81 846.0 0 81 269 101 846.0 0 101 270 74 846.0 0 74 271 49 846.0 0 49 272 75 846.0 0 75 273 45 846.0 0 45 274 41 846.0 0 41 275 71 846.0 0 71 276 115 846.0 0 115 277 61 846.0 0 61 278 34 846.0 0 34 279 42 846.0 0 42 280 83 846.0 0 83 281 146 846.0 0 146 282 78 846.0 0 78 283 45 846.0 0 45 284 73 846.0 0 73 285 51 846.0 0 51 286 41 846.0 0 41 287 90 846.0 0 90 288 86 846.0 0 86 289 82 846.0 0 82 290 64 846.0 0 64 291 39 846.0 0 39 292 81 846.0 0 81 293 252 846.0 0 252 294 262 846.0 0 262 295 82 846.0 0 82 296 55 846.0 0 55 297 38 846.0 0 38 298 50 846.0 0 50 299 37 846.0 0 37 300 31 846.0 0 31 301 38 846.0 0 38 Finished in 1.80 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 36,966 Reads with adapters: 15,467 (41.8%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 15,458 (41.8%) Total basepairs processed: 9,455,928 bp Total written (filtered): 4,199,156 bp (44.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15467 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 2 0 1 19 46 0.0 3 0 43 2 1 20 14913 0.0 4 14137 681 65 22 8 21 418 0.0 4 381 30 1 6 22 5 0.0 4 0 2 3 23 11 0.0 4 0 0 0 7 4 24 1 0.0 4 0 0 0 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 1 36 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 52 0.0 4 0 0 0 0 52 128 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 158 1 0.0 4 1 184 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 15,458 Reads with adapters: 983 (6.4%) Reads written (passing filters): 15,458 (100.0%) Total basepairs processed: 4,199,156 bp Total written (filtered): 4,166,291 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 983 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.9% C: 3.9% G: 81.9% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 2 60.4 0 2 8 3 0.2 0 3 9 180 0.1 0 18 162 10 434 0.0 1 16 418 11 266 0.0 1 0 266 12 4 0.0 1 0 4 150 1 0.0 1 0 1 208 4 0.0 1 3 1 257 85 0.0 1 79 6 261 1 0.0 1 1 269 1 0.0 1 1 271 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.