This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.44 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 64,586 (1.9%) Reads that were too short: 10,212 (0.3%) Reads written (passing filters): 54,374 (1.6%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 12,032,893 bp (1.2%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 64586 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 843 846.0 0 843 7 277 846.0 0 277 8 14778 846.0 0 14778 9 13684 846.0 0 13684 10 23 846.0 0 23 11 6 846.0 0 6 12 1 846.0 0 1 13 7 846.0 0 7 14 3 846.0 0 3 15 4 846.0 0 4 16 5 846.0 0 5 17 5 846.0 0 5 18 15 846.0 0 15 19 11 846.0 0 11 20 18 846.0 0 18 21 11 846.0 0 11 22 1 846.0 0 1 23 8 846.0 0 8 24 1 846.0 0 1 25 6 846.0 0 6 26 2 846.0 0 2 27 7 846.0 0 7 28 5 846.0 0 5 29 3 846.0 0 3 30 6 846.0 0 6 31 5 846.0 0 5 32 6 846.0 0 6 33 7 846.0 0 7 34 2 846.0 0 2 35 8 846.0 0 8 36 3 846.0 0 3 37 4 846.0 0 4 38 4 846.0 0 4 39 5 846.0 0 5 40 5 846.0 0 5 41 5 846.0 0 5 42 5 846.0 0 5 43 10 846.0 0 10 44 10 846.0 0 10 45 44 846.0 0 44 46 11 846.0 0 11 47 1 846.0 0 1 48 8 846.0 0 8 49 7 846.0 0 7 50 12 846.0 0 12 51 9 846.0 0 9 52 2 846.0 0 2 53 4 846.0 0 4 54 10 846.0 0 10 55 3 846.0 0 3 56 6 846.0 0 6 57 3 846.0 0 3 58 9 846.0 0 9 59 4 846.0 0 4 60 16 846.0 0 16 61 2 846.0 0 2 62 7 846.0 0 7 63 5 846.0 0 5 64 7 846.0 0 7 65 8 846.0 0 8 66 22 846.0 0 22 67 5 846.0 0 5 68 5 846.0 0 5 69 4 846.0 0 4 70 4 846.0 0 4 71 4 846.0 0 4 72 10 846.0 0 10 73 17 846.0 0 17 74 26 846.0 0 26 75 36 846.0 0 36 76 80 846.0 0 80 77 2100 846.0 0 2100 78 421 846.0 0 421 79 624 846.0 0 624 80 4 846.0 0 4 81 3 846.0 0 3 82 5 846.0 0 5 83 10 846.0 0 10 84 10 846.0 0 10 85 6 846.0 0 6 86 10 846.0 0 10 87 4 846.0 0 4 88 13 846.0 0 13 89 12 846.0 0 12 90 29 846.0 0 29 91 37 846.0 0 37 92 48 846.0 0 48 93 48 846.0 0 48 94 26 846.0 0 26 95 45 846.0 0 45 96 35 846.0 0 35 97 39 846.0 0 39 98 48 846.0 0 48 99 16 846.0 0 16 100 12 846.0 0 12 101 8 846.0 0 8 102 22 846.0 0 22 103 1 846.0 0 1 105 7 846.0 0 7 106 24 846.0 0 24 107 18 846.0 0 18 108 10 846.0 0 10 109 12 846.0 0 12 110 9 846.0 0 9 111 8 846.0 0 8 112 7 846.0 0 7 113 17 846.0 0 17 114 67 846.0 0 67 115 84 846.0 0 84 116 51 846.0 0 51 117 32 846.0 0 32 118 27 846.0 0 27 119 12 846.0 0 12 120 13 846.0 0 13 121 22 846.0 0 22 122 38 846.0 0 38 123 29 846.0 0 29 124 17 846.0 0 17 125 11 846.0 0 11 126 34 846.0 0 34 127 25 846.0 0 25 128 39 846.0 0 39 129 81 846.0 0 81 130 15 846.0 0 15 131 15 846.0 0 15 132 12 846.0 0 12 133 22 846.0 0 22 134 48 846.0 0 48 135 165 846.0 0 165 136 193 846.0 0 193 137 103 846.0 0 103 138 39 846.0 0 39 139 147 846.0 0 147 140 104 846.0 0 104 141 36 846.0 0 36 142 234 846.0 0 234 143 46 846.0 0 46 144 29 846.0 0 29 145 15 846.0 0 15 146 26 846.0 0 26 147 17 846.0 0 17 148 80 846.0 0 80 149 24 846.0 0 24 150 30 846.0 0 30 151 20 846.0 0 20 152 20 846.0 0 20 153 28 846.0 0 28 154 33 846.0 0 33 155 35 846.0 0 35 156 31 846.0 0 31 157 38 846.0 0 38 158 110 846.0 0 110 159 31 846.0 0 31 160 18 846.0 0 18 161 26 846.0 0 26 162 36 846.0 0 36 163 43 846.0 0 43 164 50 846.0 0 50 165 32 846.0 0 32 166 50 846.0 0 50 167 42 846.0 0 42 168 34 846.0 0 34 169 33 846.0 0 33 170 27 846.0 0 27 171 36 846.0 0 36 172 93 846.0 0 93 173 207 846.0 0 207 174 223 846.0 0 223 175 373 846.0 0 373 176 68 846.0 0 68 177 71 846.0 0 71 178 85 846.0 0 85 179 52 846.0 0 52 180 235 846.0 0 235 181 348 846.0 0 348 182 225 846.0 0 225 183 59 846.0 0 59 184 54 846.0 0 54 185 50 846.0 0 50 186 47 846.0 0 47 187 82 846.0 0 82 188 93 846.0 0 93 189 156 846.0 0 156 190 331 846.0 0 331 191 2927 846.0 0 2927 192 9074 846.0 0 9074 193 2346 846.0 0 2346 194 95 846.0 0 95 195 43 846.0 0 43 196 45 846.0 0 45 197 78 846.0 0 78 198 42 846.0 0 42 199 63 846.0 0 63 200 58 846.0 0 58 201 48 846.0 0 48 202 287 846.0 0 287 203 264 846.0 0 264 204 223 846.0 0 223 205 164 846.0 0 164 206 29 846.0 0 29 207 40 846.0 0 40 208 84 846.0 0 84 209 62 846.0 0 62 210 58 846.0 0 58 211 26 846.0 0 26 212 17 846.0 0 17 213 22 846.0 0 22 214 21 846.0 0 21 215 26 846.0 0 26 216 39 846.0 0 39 217 32 846.0 0 32 218 22 846.0 0 22 219 23 846.0 0 23 220 18 846.0 0 18 221 36 846.0 0 36 222 17 846.0 0 17 223 18 846.0 0 18 224 19 846.0 0 19 225 38 846.0 0 38 226 34 846.0 0 34 227 31 846.0 0 31 228 23 846.0 0 23 229 24 846.0 0 24 230 20 846.0 0 20 231 24 846.0 0 24 232 25 846.0 0 25 233 26 846.0 0 26 234 43 846.0 0 43 235 49 846.0 0 49 236 28 846.0 0 28 237 23 846.0 0 23 238 30 846.0 0 30 239 13 846.0 0 13 240 13 846.0 0 13 241 12 846.0 0 12 242 21 846.0 0 21 243 6 846.0 0 6 244 19 846.0 0 19 245 16 846.0 0 16 246 21 846.0 0 21 247 18 846.0 0 18 248 24 846.0 0 24 249 45 846.0 0 45 250 110 846.0 0 110 251 58 846.0 0 58 252 59 846.0 0 59 253 56 846.0 0 56 254 70 846.0 0 70 255 34 846.0 0 34 256 26 846.0 0 26 257 28 846.0 0 28 258 239 846.0 0 239 259 476 846.0 0 476 260 251 846.0 0 251 261 315 846.0 0 315 262 356 846.0 0 356 263 136 846.0 0 136 264 499 846.0 0 499 265 203 846.0 0 203 266 501 846.0 0 501 267 24 846.0 0 24 268 37 846.0 0 37 269 99 846.0 0 99 270 387 846.0 0 387 271 38 846.0 0 38 272 38 846.0 0 38 273 17 846.0 0 17 274 19 846.0 0 19 275 33 846.0 0 33 276 45 846.0 0 45 277 44 846.0 0 44 278 37 846.0 0 37 279 29 846.0 0 29 280 27 846.0 0 27 281 67 846.0 0 67 282 32 846.0 0 32 283 59 846.0 0 59 284 274 846.0 0 274 285 291 846.0 0 291 286 487 846.0 0 487 287 39 846.0 0 39 288 60 846.0 0 60 289 618 846.0 0 618 290 580 846.0 0 580 291 90 846.0 0 90 292 43 846.0 0 43 293 65 846.0 0 65 294 19 846.0 0 19 295 381 846.0 0 381 296 273 846.0 0 273 297 304 846.0 0 304 298 34 846.0 0 34 299 14 846.0 0 14 300 11 846.0 0 11 301 7 846.0 0 7 Finished in 2.22 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 54,374 Reads with adapters: 15,320 (28.2%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 15,309 (28.2%) Total basepairs processed: 12,032,893 bp Total written (filtered): 4,222,700 bp (35.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15320 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 7 0.0 3 0 0 6 1 16 119 0.0 3 0 111 2 6 17 14439 0.0 3 14255 180 3 1 18 51 0.0 3 7 23 18 3 19 41 0.0 3 2 0 14 25 20 637 0.0 3 622 7 0 8 21 4 0.0 3 0 1 0 3 24 1 0.0 3 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 52 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 1 183 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 15,309 Reads with adapters: 212 (1.4%) Reads written (passing filters): 15,309 (100.0%) Total basepairs processed: 4,222,700 bp Total written (filtered): 4,222,064 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 212 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 71.2% C: 17.5% G: 8.5% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 212 239.2 0 212