This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.13 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 353,366 (10.2%) Reads that were too short: 25,174 (0.7%) Reads written (passing filters): 328,192 (9.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 54,478,444 bp (5.2%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 353366 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 160 846.0 0 160 7 9397 846.0 0 9397 8 8468 846.0 0 8468 9 24 846.0 0 24 10 10 846.0 0 10 11 22 846.0 0 22 12 30 846.0 0 30 13 72 846.0 0 72 14 12 846.0 0 12 15 9 846.0 0 9 16 9 846.0 0 9 17 12 846.0 0 12 18 12 846.0 0 12 19 8 846.0 0 8 20 17 846.0 0 17 21 10 846.0 0 10 22 3 846.0 0 3 23 8 846.0 0 8 24 9 846.0 0 9 25 11 846.0 0 11 26 9 846.0 0 9 27 22 846.0 0 22 28 19 846.0 0 19 29 6 846.0 0 6 30 9 846.0 0 9 31 11 846.0 0 11 32 25 846.0 0 25 33 7 846.0 0 7 34 23 846.0 0 23 35 42 846.0 0 42 36 13 846.0 0 13 37 5 846.0 0 5 38 163 846.0 0 163 39 15 846.0 0 15 40 8 846.0 0 8 41 3 846.0 0 3 42 9 846.0 0 9 43 19 846.0 0 19 44 9 846.0 0 9 45 3 846.0 0 3 46 7 846.0 0 7 47 11 846.0 0 11 48 12 846.0 0 12 49 12 846.0 0 12 50 6 846.0 0 6 51 15 846.0 0 15 52 2 846.0 0 2 53 6 846.0 0 6 54 12 846.0 0 12 55 14 846.0 0 14 56 16 846.0 0 16 57 10 846.0 0 10 58 11 846.0 0 11 59 19 846.0 0 19 60 12 846.0 0 12 61 5 846.0 0 5 62 13 846.0 0 13 63 10 846.0 0 10 64 15 846.0 0 15 65 9 846.0 0 9 66 18 846.0 0 18 67 10 846.0 0 10 68 10 846.0 0 10 69 11 846.0 0 11 70 16 846.0 0 16 71 34 846.0 0 34 72 64 846.0 0 64 73 35 846.0 0 35 74 32 846.0 0 32 75 85 846.0 0 85 76 68 846.0 0 68 77 97 846.0 0 97 78 107 846.0 0 107 79 50 846.0 0 50 80 189 846.0 0 189 81 155 846.0 0 155 82 147 846.0 0 147 83 210 846.0 0 210 84 147 846.0 0 147 85 160 846.0 0 160 86 90 846.0 0 90 87 93 846.0 0 93 88 94 846.0 0 94 89 365 846.0 0 365 90 472 846.0 0 472 91 432 846.0 0 432 92 356 846.0 0 356 93 494 846.0 0 494 94 110 846.0 0 110 95 189 846.0 0 189 96 185 846.0 0 185 97 250 846.0 0 250 98 319 846.0 0 319 99 231 846.0 0 231 100 244 846.0 0 244 101 504 846.0 0 504 102 785 846.0 0 785 103 739 846.0 0 739 104 394 846.0 0 394 105 165 846.0 0 165 106 149 846.0 0 149 107 99 846.0 0 99 108 131 846.0 0 131 109 420 846.0 0 420 110 442 846.0 0 442 111 523 846.0 0 523 112 351 846.0 0 351 113 402 846.0 0 402 114 385 846.0 0 385 115 441 846.0 0 441 116 221 846.0 0 221 117 142 846.0 0 142 118 103 846.0 0 103 119 100 846.0 0 100 120 117 846.0 0 117 121 123 846.0 0 123 122 396 846.0 0 396 123 589 846.0 0 589 124 377 846.0 0 377 125 618 846.0 0 618 126 233 846.0 0 233 127 375 846.0 0 375 128 347 846.0 0 347 129 412 846.0 0 412 130 360 846.0 0 360 131 284 846.0 0 284 132 346 846.0 0 346 133 241 846.0 0 241 134 255 846.0 0 255 135 376 846.0 0 376 136 503 846.0 0 503 137 937 846.0 0 937 138 925 846.0 0 925 139 1547 846.0 0 1547 140 2555 846.0 0 2555 141 2899 846.0 0 2899 142 52642 846.0 0 52642 143 182178 846.0 0 182178 144 23371 846.0 0 23371 145 373 846.0 0 373 146 283 846.0 0 283 147 484 846.0 0 484 148 408 846.0 0 408 149 307 846.0 0 307 150 249 846.0 0 249 151 231 846.0 0 231 152 186 846.0 0 186 153 201 846.0 0 201 154 132 846.0 0 132 155 536 846.0 0 536 156 367 846.0 0 367 157 329 846.0 0 329 158 289 846.0 0 289 159 212 846.0 0 212 160 327 846.0 0 327 161 323 846.0 0 323 162 3060 846.0 0 3060 163 6026 846.0 0 6026 164 1452 846.0 0 1452 165 189 846.0 0 189 166 157 846.0 0 157 167 310 846.0 0 310 168 542 846.0 0 542 169 305 846.0 0 305 170 207 846.0 0 207 171 144 846.0 0 144 172 93 846.0 0 93 173 138 846.0 0 138 174 99 846.0 0 99 175 218 846.0 0 218 176 448 846.0 0 448 177 452 846.0 0 452 178 423 846.0 0 423 179 185 846.0 0 185 180 131 846.0 0 131 181 145 846.0 0 145 182 122 846.0 0 122 183 214 846.0 0 214 184 297 846.0 0 297 185 220 846.0 0 220 186 188 846.0 0 188 187 143 846.0 0 143 188 269 846.0 0 269 189 269 846.0 0 269 190 279 846.0 0 279 191 187 846.0 0 187 192 108 846.0 0 108 193 193 846.0 0 193 194 154 846.0 0 154 195 184 846.0 0 184 196 225 846.0 0 225 197 267 846.0 0 267 198 486 846.0 0 486 199 157 846.0 0 157 200 150 846.0 0 150 201 108 846.0 0 108 202 181 846.0 0 181 203 379 846.0 0 379 204 318 846.0 0 318 205 250 846.0 0 250 206 329 846.0 0 329 207 279 846.0 0 279 208 217 846.0 0 217 209 297 846.0 0 297 210 353 846.0 0 353 211 353 846.0 0 353 212 347 846.0 0 347 213 277 846.0 0 277 214 385 846.0 0 385 215 328 846.0 0 328 216 330 846.0 0 330 217 304 846.0 0 304 218 240 846.0 0 240 219 213 846.0 0 213 220 124 846.0 0 124 221 147 846.0 0 147 222 266 846.0 0 266 223 423 846.0 0 423 224 579 846.0 0 579 225 257 846.0 0 257 226 248 846.0 0 248 227 290 846.0 0 290 228 217 846.0 0 217 229 178 846.0 0 178 230 184 846.0 0 184 231 127 846.0 0 127 232 286 846.0 0 286 233 316 846.0 0 316 234 408 846.0 0 408 235 379 846.0 0 379 236 242 846.0 0 242 237 238 846.0 0 238 238 177 846.0 0 177 239 189 846.0 0 189 240 206 846.0 0 206 241 144 846.0 0 144 242 169 846.0 0 169 243 342 846.0 0 342 244 406 846.0 0 406 245 437 846.0 0 437 246 391 846.0 0 391 247 208 846.0 0 208 248 128 846.0 0 128 249 54 846.0 0 54 250 85 846.0 0 85 251 118 846.0 0 118 252 261 846.0 0 261 253 362 846.0 0 362 254 225 846.0 0 225 255 332 846.0 0 332 256 448 846.0 0 448 257 378 846.0 0 378 258 340 846.0 0 340 259 284 846.0 0 284 260 186 846.0 0 186 261 162 846.0 0 162 262 517 846.0 0 517 263 360 846.0 0 360 264 334 846.0 0 334 265 262 846.0 0 262 266 322 846.0 0 322 267 165 846.0 0 165 268 253 846.0 0 253 269 138 846.0 0 138 270 156 846.0 0 156 271 185 846.0 0 185 272 256 846.0 0 256 273 338 846.0 0 338 274 168 846.0 0 168 275 170 846.0 0 170 276 117 846.0 0 117 277 112 846.0 0 112 278 240 846.0 0 240 279 349 846.0 0 349 280 222 846.0 0 222 281 109 846.0 0 109 282 238 846.0 0 238 283 213 846.0 0 213 284 302 846.0 0 302 285 340 846.0 0 340 286 214 846.0 0 214 287 211 846.0 0 211 288 79 846.0 0 79 289 81 846.0 0 81 290 99 846.0 0 99 291 171 846.0 0 171 292 363 846.0 0 363 293 297 846.0 0 297 294 181 846.0 0 181 295 236 846.0 0 236 296 216 846.0 0 216 297 162 846.0 0 162 298 174 846.0 0 174 299 173 846.0 0 173 300 170 846.0 0 170 301 160 846.0 0 160 Finished in 11.31 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 328,192 Reads with adapters: 8,674 (2.6%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 8,651 (2.6%) Total basepairs processed: 54,478,444 bp Total written (filtered): 2,356,543 bp (4.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8674 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 0 1 19 29 0.0 3 0 25 1 2 1 20 8542 0.0 4 8354 154 22 5 7 21 26 0.0 4 4 22 22 2 0.0 4 0 0 2 23 3 0.0 4 0 0 0 3 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 1 33 1 0.0 4 0 0 0 0 1 36 2 0.0 4 0 0 0 0 2 37 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 36 0.0 4 0 0 0 0 36 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 8,651 Reads with adapters: 982 (11.4%) Reads written (passing filters): 8,651 (100.0%) Total basepairs processed: 2,356,543 bp Total written (filtered): 2,324,579 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 982 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.3% C: 3.8% G: 85.0% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 135.2 0 1 6 1 2.1 0 1 9 6 0.0 0 0 6 10 62 0.0 1 5 57 11 823 0.0 1 5 818 12 1 0.0 1 0 1 14 1 0.0 1 0 1 151 2 0.0 1 2 209 1 0.0 1 1 224 5 0.0 1 5 259 2 0.0 1 2 260 73 0.0 1 72 1 262 1 0.0 1 1 268 1 0.0 1 1 271 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.