This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.60 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 232,750 (6.7%) Reads that were too short: 47,038 (1.4%) Reads written (passing filters): 185,712 (5.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 46,321,458 bp (4.4%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 232750 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 758 846.0 0 758 7 9907 846.0 0 9907 8 415 846.0 0 415 9 4685 846.0 0 4685 10 784 846.0 0 784 11 971 846.0 0 971 12 21570 846.0 0 21570 13 32579 846.0 0 32579 14 53486 846.0 0 53486 15 310 846.0 0 310 16 29 846.0 0 29 17 38 846.0 0 38 18 30 846.0 0 30 19 27 846.0 0 27 20 26 846.0 0 26 21 24 846.0 0 24 22 32 846.0 0 32 23 40 846.0 0 40 24 68 846.0 0 68 25 37 846.0 0 37 26 55 846.0 0 55 27 113 846.0 0 113 28 29 846.0 0 29 29 28 846.0 0 28 30 36 846.0 0 36 31 25 846.0 0 25 32 30 846.0 0 30 33 39 846.0 0 39 34 32 846.0 0 32 35 39 846.0 0 39 36 49 846.0 0 49 37 77 846.0 0 77 38 2316 846.0 0 2316 39 45 846.0 0 45 40 28 846.0 0 28 41 21 846.0 0 21 42 21 846.0 0 21 43 64 846.0 0 64 44 64 846.0 0 64 45 29 846.0 0 29 46 21 846.0 0 21 47 14 846.0 0 14 48 16 846.0 0 16 49 17 846.0 0 17 50 22 846.0 0 22 51 21 846.0 0 21 52 21 846.0 0 21 53 27 846.0 0 27 54 23 846.0 0 23 55 21 846.0 0 21 56 30 846.0 0 30 57 18 846.0 0 18 58 28 846.0 0 28 59 19 846.0 0 19 60 23 846.0 0 23 61 23 846.0 0 23 62 24 846.0 0 24 63 26 846.0 0 26 64 27 846.0 0 27 65 37 846.0 0 37 66 24 846.0 0 24 67 36 846.0 0 36 68 38 846.0 0 38 69 22 846.0 0 22 70 30 846.0 0 30 71 48 846.0 0 48 72 70 846.0 0 70 73 199 846.0 0 199 74 108 846.0 0 108 75 98 846.0 0 98 76 98 846.0 0 98 77 125 846.0 0 125 78 124 846.0 0 124 79 119 846.0 0 119 80 65 846.0 0 65 81 86 846.0 0 86 82 104 846.0 0 104 83 141 846.0 0 141 84 133 846.0 0 133 85 105 846.0 0 105 86 168 846.0 0 168 87 190 846.0 0 190 88 233 846.0 0 233 89 273 846.0 0 273 90 331 846.0 0 331 91 306 846.0 0 306 92 262 846.0 0 262 93 261 846.0 0 261 94 483 846.0 0 483 95 406 846.0 0 406 96 471 846.0 0 471 97 378 846.0 0 378 98 159 846.0 0 159 99 119 846.0 0 119 100 105 846.0 0 105 101 109 846.0 0 109 102 111 846.0 0 111 103 121 846.0 0 121 104 3232 846.0 0 3232 105 874 846.0 0 874 106 667 846.0 0 667 107 295 846.0 0 295 108 891 846.0 0 891 109 558 846.0 0 558 110 408 846.0 0 408 111 340 846.0 0 340 112 373 846.0 0 373 113 345 846.0 0 345 114 240 846.0 0 240 115 178 846.0 0 178 116 158 846.0 0 158 117 125 846.0 0 125 118 119 846.0 0 119 119 237 846.0 0 237 120 576 846.0 0 576 121 473 846.0 0 473 122 260 846.0 0 260 123 162 846.0 0 162 124 278 846.0 0 278 125 419 846.0 0 419 126 1103 846.0 0 1103 127 2481 846.0 0 2481 128 2948 846.0 0 2948 129 613 846.0 0 613 130 195 846.0 0 195 131 148 846.0 0 148 132 166 846.0 0 166 133 292 846.0 0 292 134 375 846.0 0 375 135 693 846.0 0 693 136 543 846.0 0 543 137 286 846.0 0 286 138 676 846.0 0 676 139 753 846.0 0 753 140 1058 846.0 0 1058 141 518 846.0 0 518 142 433 846.0 0 433 143 229 846.0 0 229 144 280 846.0 0 280 145 413 846.0 0 413 146 619 846.0 0 619 147 538 846.0 0 538 148 363 846.0 0 363 149 299 846.0 0 299 150 222 846.0 0 222 151 788 846.0 0 788 152 1855 846.0 0 1855 153 864 846.0 0 864 154 577 846.0 0 577 155 405 846.0 0 405 156 338 846.0 0 338 157 264 846.0 0 264 158 680 846.0 0 680 159 1824 846.0 0 1824 160 615 846.0 0 615 161 674 846.0 0 674 162 376 846.0 0 376 163 303 846.0 0 303 164 299 846.0 0 299 165 406 846.0 0 406 166 384 846.0 0 384 167 353 846.0 0 353 168 226 846.0 0 226 169 239 846.0 0 239 170 257 846.0 0 257 171 198 846.0 0 198 172 277 846.0 0 277 173 394 846.0 0 394 174 631 846.0 0 631 175 452 846.0 0 452 176 357 846.0 0 357 177 265 846.0 0 265 178 217 846.0 0 217 179 260 846.0 0 260 180 323 846.0 0 323 181 601 846.0 0 601 182 436 846.0 0 436 183 356 846.0 0 356 184 264 846.0 0 264 185 374 846.0 0 374 186 421 846.0 0 421 187 366 846.0 0 366 188 357 846.0 0 357 189 223 846.0 0 223 190 358 846.0 0 358 191 412 846.0 0 412 192 451 846.0 0 451 193 327 846.0 0 327 194 530 846.0 0 530 195 220 846.0 0 220 196 211 846.0 0 211 197 226 846.0 0 226 198 377 846.0 0 377 199 366 846.0 0 366 200 292 846.0 0 292 201 318 846.0 0 318 202 408 846.0 0 408 203 604 846.0 0 604 204 398 846.0 0 398 205 259 846.0 0 259 206 381 846.0 0 381 207 591 846.0 0 591 208 510 846.0 0 510 209 351 846.0 0 351 210 274 846.0 0 274 211 236 846.0 0 236 212 242 846.0 0 242 213 316 846.0 0 316 214 434 846.0 0 434 215 470 846.0 0 470 216 421 846.0 0 421 217 316 846.0 0 316 218 309 846.0 0 309 219 363 846.0 0 363 220 702 846.0 0 702 221 474 846.0 0 474 222 350 846.0 0 350 223 266 846.0 0 266 224 592 846.0 0 592 225 372 846.0 0 372 226 721 846.0 0 721 227 269 846.0 0 269 228 392 846.0 0 392 229 312 846.0 0 312 230 353 846.0 0 353 231 394 846.0 0 394 232 354 846.0 0 354 233 468 846.0 0 468 234 509 846.0 0 509 235 403 846.0 0 403 236 421 846.0 0 421 237 236 846.0 0 236 238 228 846.0 0 228 239 274 846.0 0 274 240 513 846.0 0 513 241 454 846.0 0 454 242 573 846.0 0 573 243 637 846.0 0 637 244 446 846.0 0 446 245 326 846.0 0 326 246 258 846.0 0 258 247 353 846.0 0 353 248 189 846.0 0 189 249 223 846.0 0 223 250 107 846.0 0 107 251 434 846.0 0 434 252 614 846.0 0 614 253 722 846.0 0 722 254 372 846.0 0 372 255 387 846.0 0 387 256 388 846.0 0 388 257 446 846.0 0 446 258 335 846.0 0 335 259 184 846.0 0 184 260 219 846.0 0 219 261 371 846.0 0 371 262 471 846.0 0 471 263 440 846.0 0 440 264 343 846.0 0 343 265 269 846.0 0 269 266 349 846.0 0 349 267 935 846.0 0 935 268 748 846.0 0 748 269 580 846.0 0 580 270 273 846.0 0 273 271 385 846.0 0 385 272 356 846.0 0 356 273 298 846.0 0 298 274 414 846.0 0 414 275 370 846.0 0 370 276 315 846.0 0 315 277 635 846.0 0 635 278 899 846.0 0 899 279 1640 846.0 0 1640 280 871 846.0 0 871 281 1228 846.0 0 1228 282 1887 846.0 0 1887 283 868 846.0 0 868 284 651 846.0 0 651 285 326 846.0 0 326 286 387 846.0 0 387 287 396 846.0 0 396 288 2922 846.0 0 2922 289 387 846.0 0 387 290 291 846.0 0 291 291 299 846.0 0 299 292 446 846.0 0 446 293 444 846.0 0 444 294 668 846.0 0 668 295 421 846.0 0 421 296 308 846.0 0 308 297 194 846.0 0 194 298 163 846.0 0 163 299 193 846.0 0 193 300 257 846.0 0 257 301 187 846.0 0 187 Finished in 8.56 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 185,712 Reads with adapters: 4,630 (2.5%) Reads that were too short: 63 (0.0%) Reads written (passing filters): 4,567 (2.5%) Total basepairs processed: 46,321,458 bp Total written (filtered): 1,227,464 bp (2.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4630 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 1 19 16 0.0 3 0 14 1 1 20 4188 0.0 4 3949 212 17 8 2 21 13 0.0 4 3 8 2 22 5 0.0 4 0 1 1 0 3 23 206 0.0 4 197 6 0 3 24 2 0.0 4 1 0 0 0 1 28 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 7 0.0 4 0 0 0 0 7 117 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 122 117 0.0 4 0 0 0 0 117 123 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 137 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 148 5 0.0 4 0 0 0 0 5 154 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 223 6 0.0 4 0 0 0 1 5 224 3 0.0 4 0 0 0 0 3 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 277 3 0.0 4 0 0 0 0 3 278 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 286 2 0.0 4 0 0 0 0 2 287 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 4,567 Reads with adapters: 907 (19.9%) Reads written (passing filters): 4,567 (100.0%) Total basepairs processed: 1,227,464 bp Total written (filtered): 1,054,092 bp (85.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 907 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 67.9% C: 2.8% G: 28.7% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 49 0.0 0 5 44 10 100 0.0 1 8 92 11 93 0.0 1 1 92 13 1 0.0 1 0 1 150 1 0.0 1 1 152 1 0.0 1 1 208 3 0.0 1 3 211 1 0.0 1 1 254 2 0.0 1 2 256 3 0.0 1 3 257 506 0.0 1 482 24 258 12 0.0 1 12 259 8 0.0 1 7 1 260 3 0.0 1 3 261 41 0.0 1 36 5 262 73 0.0 1 72 1 263 1 0.0 1 1 264 2 0.0 1 2 266 4 0.0 1 4 269 1 0.0 1 1 270 1 0.0 1 1 271 1 0.0 1 1