This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.02 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 127,377 (3.7%) Reads that were too short: 48,738 (1.4%) Reads written (passing filters): 78,639 (2.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 15,372,245 bp (1.5%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 127377 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 281 846.0 0 281 7 6485 846.0 0 6485 8 138 846.0 0 138 9 13641 846.0 0 13641 10 64 846.0 0 64 11 41 846.0 0 41 12 25 846.0 0 25 13 31 846.0 0 31 14 30 846.0 0 30 15 21 846.0 0 21 16 30 846.0 0 30 17 27 846.0 0 27 18 24 846.0 0 24 19 20 846.0 0 20 20 37 846.0 0 37 21 30 846.0 0 30 22 33 846.0 0 33 23 33 846.0 0 33 24 21 846.0 0 21 25 29 846.0 0 29 26 25 846.0 0 25 27 40 846.0 0 40 28 23 846.0 0 23 29 32 846.0 0 32 30 31 846.0 0 31 31 23 846.0 0 23 32 31 846.0 0 31 33 30 846.0 0 30 34 24 846.0 0 24 35 63 846.0 0 63 36 55 846.0 0 55 37 38 846.0 0 38 38 28 846.0 0 28 39 25 846.0 0 25 40 45 846.0 0 45 41 13 846.0 0 13 42 29 846.0 0 29 43 15 846.0 0 15 44 24 846.0 0 24 45 29 846.0 0 29 46 46 846.0 0 46 47 29 846.0 0 29 48 28 846.0 0 28 49 38 846.0 0 38 50 38 846.0 0 38 51 29 846.0 0 29 52 38 846.0 0 38 53 45 846.0 0 45 54 33 846.0 0 33 55 59 846.0 0 59 56 31 846.0 0 31 57 20 846.0 0 20 58 41 846.0 0 41 59 44 846.0 0 44 60 37 846.0 0 37 61 36 846.0 0 36 62 26 846.0 0 26 63 30 846.0 0 30 64 35 846.0 0 35 65 39 846.0 0 39 66 28 846.0 0 28 67 38 846.0 0 38 68 43 846.0 0 43 69 43 846.0 0 43 70 53 846.0 0 53 71 36 846.0 0 36 72 38 846.0 0 38 73 157 846.0 0 157 74 1145 846.0 0 1145 75 114 846.0 0 114 76 76 846.0 0 76 77 55 846.0 0 55 78 56 846.0 0 56 79 137 846.0 0 137 80 342 846.0 0 342 81 239 846.0 0 239 82 358 846.0 0 358 83 199 846.0 0 199 84 332 846.0 0 332 85 274 846.0 0 274 86 123 846.0 0 123 87 105 846.0 0 105 88 86 846.0 0 86 89 182 846.0 0 182 90 246 846.0 0 246 91 517 846.0 0 517 92 328 846.0 0 328 93 120 846.0 0 120 94 84 846.0 0 84 95 102 846.0 0 102 96 92 846.0 0 92 97 121 846.0 0 121 98 180 846.0 0 180 99 335 846.0 0 335 100 411 846.0 0 411 101 1411 846.0 0 1411 102 1405 846.0 0 1405 103 613 846.0 0 613 104 241 846.0 0 241 105 261 846.0 0 261 106 201 846.0 0 201 107 150 846.0 0 150 108 129 846.0 0 129 109 164 846.0 0 164 110 227 846.0 0 227 111 462 846.0 0 462 112 367 846.0 0 367 113 335 846.0 0 335 114 225 846.0 0 225 115 474 846.0 0 474 116 547 846.0 0 547 117 445 846.0 0 445 118 310 846.0 0 310 119 170 846.0 0 170 120 143 846.0 0 143 121 169 846.0 0 169 122 257 846.0 0 257 123 733 846.0 0 733 124 780 846.0 0 780 125 552 846.0 0 552 126 224 846.0 0 224 127 145 846.0 0 145 128 129 846.0 0 129 129 197 846.0 0 197 130 743 846.0 0 743 131 1379 846.0 0 1379 132 931 846.0 0 931 133 605 846.0 0 605 134 301 846.0 0 301 135 248 846.0 0 248 136 445 846.0 0 445 137 643 846.0 0 643 138 544 846.0 0 544 139 371 846.0 0 371 140 240 846.0 0 240 141 486 846.0 0 486 142 669 846.0 0 669 143 656 846.0 0 656 144 679 846.0 0 679 145 801 846.0 0 801 146 337 846.0 0 337 147 236 846.0 0 236 148 457 846.0 0 457 149 913 846.0 0 913 150 769 846.0 0 769 151 446 846.0 0 446 152 507 846.0 0 507 153 330 846.0 0 330 154 495 846.0 0 495 155 829 846.0 0 829 156 877 846.0 0 877 157 899 846.0 0 899 158 738 846.0 0 738 159 437 846.0 0 437 160 302 846.0 0 302 161 305 846.0 0 305 162 527 846.0 0 527 163 411 846.0 0 411 164 504 846.0 0 504 165 354 846.0 0 354 166 298 846.0 0 298 167 412 846.0 0 412 168 422 846.0 0 422 169 735 846.0 0 735 170 1752 846.0 0 1752 171 974 846.0 0 974 172 493 846.0 0 493 173 184 846.0 0 184 174 152 846.0 0 152 175 229 846.0 0 229 176 340 846.0 0 340 177 746 846.0 0 746 178 1187 846.0 0 1187 179 1030 846.0 0 1030 180 433 846.0 0 433 181 226 846.0 0 226 182 294 846.0 0 294 183 313 846.0 0 313 184 392 846.0 0 392 185 303 846.0 0 303 186 383 846.0 0 383 187 522 846.0 0 522 188 716 846.0 0 716 189 548 846.0 0 548 190 394 846.0 0 394 191 217 846.0 0 217 192 142 846.0 0 142 193 191 846.0 0 191 194 188 846.0 0 188 195 416 846.0 0 416 196 333 846.0 0 333 197 686 846.0 0 686 198 407 846.0 0 407 199 532 846.0 0 532 200 657 846.0 0 657 201 331 846.0 0 331 202 339 846.0 0 339 203 350 846.0 0 350 204 475 846.0 0 475 205 552 846.0 0 552 206 376 846.0 0 376 207 310 846.0 0 310 208 326 846.0 0 326 209 434 846.0 0 434 210 739 846.0 0 739 211 940 846.0 0 940 212 658 846.0 0 658 213 470 846.0 0 470 214 465 846.0 0 465 215 422 846.0 0 422 216 412 846.0 0 412 217 382 846.0 0 382 218 353 846.0 0 353 219 329 846.0 0 329 220 241 846.0 0 241 221 404 846.0 0 404 222 400 846.0 0 400 223 634 846.0 0 634 224 622 846.0 0 622 225 764 846.0 0 764 226 517 846.0 0 517 227 425 846.0 0 425 228 377 846.0 0 377 229 345 846.0 0 345 230 246 846.0 0 246 231 258 846.0 0 258 232 302 846.0 0 302 233 310 846.0 0 310 234 317 846.0 0 317 235 253 846.0 0 253 236 361 846.0 0 361 237 367 846.0 0 367 238 303 846.0 0 303 239 318 846.0 0 318 240 287 846.0 0 287 241 401 846.0 0 401 242 413 846.0 0 413 243 656 846.0 0 656 244 552 846.0 0 552 245 760 846.0 0 760 246 657 846.0 0 657 247 471 846.0 0 471 248 564 846.0 0 564 249 462 846.0 0 462 250 645 846.0 0 645 251 349 846.0 0 349 252 405 846.0 0 405 253 249 846.0 0 249 254 337 846.0 0 337 255 392 846.0 0 392 256 303 846.0 0 303 257 360 846.0 0 360 258 472 846.0 0 472 259 468 846.0 0 468 260 456 846.0 0 456 261 459 846.0 0 459 262 485 846.0 0 485 263 565 846.0 0 565 264 899 846.0 0 899 265 605 846.0 0 605 266 354 846.0 0 354 267 176 846.0 0 176 268 235 846.0 0 235 269 256 846.0 0 256 270 623 846.0 0 623 271 610 846.0 0 610 272 823 846.0 0 823 273 676 846.0 0 676 274 454 846.0 0 454 275 432 846.0 0 432 276 450 846.0 0 450 277 574 846.0 0 574 278 331 846.0 0 331 279 242 846.0 0 242 280 293 846.0 0 293 281 502 846.0 0 502 282 651 846.0 0 651 283 689 846.0 0 689 284 1831 846.0 0 1831 285 2315 846.0 0 2315 286 1290 846.0 0 1290 287 523 846.0 0 523 288 221 846.0 0 221 289 393 846.0 0 393 290 282 846.0 0 282 291 427 846.0 0 427 292 305 846.0 0 305 293 284 846.0 0 284 294 259 846.0 0 259 295 248 846.0 0 248 296 370 846.0 0 370 297 565 846.0 0 565 298 706 846.0 0 706 299 657 846.0 0 657 300 541 846.0 0 541 301 382 846.0 0 382 Finished in 3.17 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 78,639 Reads with adapters: 6,610 (8.4%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 6,585 (8.4%) Total basepairs processed: 15,372,245 bp Total written (filtered): 1,796,420 bp (11.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6610 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 19 23 0.0 3 0 18 0 2 3 20 6388 0.0 4 6282 92 8 5 1 21 12 0.0 4 2 10 22 7 0.0 4 4 2 1 23 88 0.0 4 73 6 1 5 3 30 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 35 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 1 95 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 58 0.0 4 0 0 0 0 58 133 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 6,585 Reads with adapters: 182 (2.8%) Reads written (passing filters): 6,585 (100.0%) Total basepairs processed: 1,796,420 bp Total written (filtered): 1,788,336 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 182 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.4% C: 8.2% G: 75.3% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 102.9 0 3 9 2 0.0 0 0 2 10 6 0.0 1 0 6 11 128 0.0 1 0 128 12 13 0.0 1 0 13 152 5 0.0 1 4 1 179 1 0.0 1 1 210 14 0.0 1 13 1 211 1 0.0 1 1 257 4 0.0 1 4 260 1 0.0 1 1 262 1 0.0 1 1 264 2 0.0 1 2 265 1 0.0 1 1