This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 89.73 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 149,136 (4.3%) Reads that were too short: 65,553 (1.9%) Reads written (passing filters): 83,583 (2.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 22,057,075 bp (2.1%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 149136 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 337 846.0 0 337 7 718 846.0 0 718 8 30241 846.0 0 30241 9 30471 846.0 0 30471 10 57 846.0 0 57 11 16 846.0 0 16 12 8 846.0 0 8 13 12 846.0 0 12 14 18 846.0 0 18 15 15 846.0 0 15 16 17 846.0 0 17 17 16 846.0 0 16 18 21 846.0 0 21 19 50 846.0 0 50 20 21 846.0 0 21 21 38 846.0 0 38 22 44 846.0 0 44 23 21 846.0 0 21 24 16 846.0 0 16 25 22 846.0 0 22 26 24 846.0 0 24 27 20 846.0 0 20 28 22 846.0 0 22 29 40 846.0 0 40 30 16 846.0 0 16 31 13 846.0 0 13 32 28 846.0 0 28 33 65 846.0 0 65 34 23 846.0 0 23 35 20 846.0 0 20 36 214 846.0 0 214 37 19 846.0 0 19 38 36 846.0 0 36 39 14 846.0 0 14 40 14 846.0 0 14 41 9 846.0 0 9 42 16 846.0 0 16 43 13 846.0 0 13 44 9 846.0 0 9 45 12 846.0 0 12 46 10 846.0 0 10 47 20 846.0 0 20 48 14 846.0 0 14 49 16 846.0 0 16 50 20 846.0 0 20 51 44 846.0 0 44 52 16 846.0 0 16 53 15 846.0 0 15 54 44 846.0 0 44 55 23 846.0 0 23 56 45 846.0 0 45 57 147 846.0 0 147 58 35 846.0 0 35 59 50 846.0 0 50 60 25 846.0 0 25 61 40 846.0 0 40 62 23 846.0 0 23 63 16 846.0 0 16 64 32 846.0 0 32 65 22 846.0 0 22 66 11 846.0 0 11 67 28 846.0 0 28 68 13 846.0 0 13 69 77 846.0 0 77 70 111 846.0 0 111 71 49 846.0 0 49 72 29 846.0 0 29 73 39 846.0 0 39 74 40 846.0 0 40 75 72 846.0 0 72 76 70 846.0 0 70 77 26 846.0 0 26 78 50 846.0 0 50 79 45 846.0 0 45 80 72 846.0 0 72 81 79 846.0 0 79 82 59 846.0 0 59 83 83 846.0 0 83 84 51 846.0 0 51 85 95 846.0 0 95 86 88 846.0 0 88 87 132 846.0 0 132 88 232 846.0 0 232 89 4783 846.0 0 4783 90 1370 846.0 0 1370 91 1020 846.0 0 1020 92 39 846.0 0 39 93 77 846.0 0 77 94 82 846.0 0 82 95 67 846.0 0 67 96 170 846.0 0 170 97 200 846.0 0 200 98 250 846.0 0 250 99 141 846.0 0 141 100 127 846.0 0 127 101 169 846.0 0 169 102 110 846.0 0 110 103 93 846.0 0 93 104 32 846.0 0 32 105 27 846.0 0 27 106 16 846.0 0 16 107 20 846.0 0 20 108 37 846.0 0 37 109 32 846.0 0 32 110 29 846.0 0 29 111 55 846.0 0 55 112 45 846.0 0 45 113 31 846.0 0 31 114 46 846.0 0 46 115 47 846.0 0 47 116 19 846.0 0 19 117 29 846.0 0 29 118 39 846.0 0 39 119 39 846.0 0 39 120 46 846.0 0 46 121 47 846.0 0 47 122 35 846.0 0 35 123 31 846.0 0 31 124 43 846.0 0 43 125 39 846.0 0 39 126 69 846.0 0 69 127 53 846.0 0 53 128 48 846.0 0 48 129 51 846.0 0 51 130 45 846.0 0 45 131 56 846.0 0 56 132 73 846.0 0 73 133 205 846.0 0 205 134 70 846.0 0 70 135 63 846.0 0 63 136 47 846.0 0 47 137 64 846.0 0 64 138 50 846.0 0 50 139 118 846.0 0 118 140 91 846.0 0 91 141 68 846.0 0 68 142 38 846.0 0 38 143 41 846.0 0 41 144 50 846.0 0 50 145 83 846.0 0 83 146 96 846.0 0 96 147 100 846.0 0 100 148 455 846.0 0 455 149 282 846.0 0 282 150 362 846.0 0 362 151 52 846.0 0 52 152 28 846.0 0 28 153 56 846.0 0 56 154 67 846.0 0 67 155 48 846.0 0 48 156 73 846.0 0 73 157 99 846.0 0 99 158 76 846.0 0 76 159 78 846.0 0 78 160 62 846.0 0 62 161 75 846.0 0 75 162 61 846.0 0 61 163 51 846.0 0 51 164 64 846.0 0 64 165 63 846.0 0 63 166 63 846.0 0 63 167 50 846.0 0 50 168 516 846.0 0 516 169 198 846.0 0 198 170 401 846.0 0 401 171 33 846.0 0 33 172 30 846.0 0 30 173 63 846.0 0 63 174 69 846.0 0 69 175 75 846.0 0 75 176 943 846.0 0 943 177 1125 846.0 0 1125 178 509 846.0 0 509 179 61 846.0 0 61 180 46 846.0 0 46 181 48 846.0 0 48 182 77 846.0 0 77 183 51 846.0 0 51 184 72 846.0 0 72 185 61 846.0 0 61 186 90 846.0 0 90 187 43 846.0 0 43 188 46 846.0 0 46 189 39 846.0 0 39 190 39 846.0 0 39 191 41 846.0 0 41 192 50 846.0 0 50 193 90 846.0 0 90 194 106 846.0 0 106 195 99 846.0 0 99 196 72 846.0 0 72 197 38 846.0 0 38 198 57 846.0 0 57 199 40 846.0 0 40 200 51 846.0 0 51 201 84 846.0 0 84 202 68 846.0 0 68 203 88 846.0 0 88 204 98 846.0 0 98 205 144 846.0 0 144 206 81 846.0 0 81 207 286 846.0 0 286 208 397 846.0 0 397 209 251 846.0 0 251 210 131 846.0 0 131 211 66 846.0 0 66 212 94 846.0 0 94 213 92 846.0 0 92 214 177 846.0 0 177 215 163 846.0 0 163 216 126 846.0 0 126 217 130 846.0 0 130 218 165 846.0 0 165 219 282 846.0 0 282 220 3665 846.0 0 3665 221 3371 846.0 0 3371 222 2010 846.0 0 2010 223 81 846.0 0 81 224 72 846.0 0 72 225 82 846.0 0 82 226 88 846.0 0 88 227 73 846.0 0 73 228 93 846.0 0 93 229 47 846.0 0 47 230 108 846.0 0 108 231 106 846.0 0 106 232 71 846.0 0 71 233 94 846.0 0 94 234 96 846.0 0 96 235 117 846.0 0 117 236 71 846.0 0 71 237 72 846.0 0 72 238 133 846.0 0 133 239 157 846.0 0 157 240 132 846.0 0 132 241 147 846.0 0 147 242 136 846.0 0 136 243 116 846.0 0 116 244 169 846.0 0 169 245 145 846.0 0 145 246 199 846.0 0 199 247 73 846.0 0 73 248 73 846.0 0 73 249 46 846.0 0 46 250 30 846.0 0 30 251 67 846.0 0 67 252 52 846.0 0 52 253 81 846.0 0 81 254 136 846.0 0 136 255 221 846.0 0 221 256 416 846.0 0 416 257 386 846.0 0 386 258 103 846.0 0 103 259 87 846.0 0 87 260 75 846.0 0 75 261 65 846.0 0 65 262 50 846.0 0 50 263 63 846.0 0 63 264 85 846.0 0 85 265 125 846.0 0 125 266 199 846.0 0 199 267 128 846.0 0 128 268 167 846.0 0 167 269 437 846.0 0 437 270 508 846.0 0 508 271 364 846.0 0 364 272 217 846.0 0 217 273 217 846.0 0 217 274 363 846.0 0 363 275 500 846.0 0 500 276 860 846.0 0 860 277 76 846.0 0 76 278 106 846.0 0 106 279 251 846.0 0 251 280 447 846.0 0 447 281 720 846.0 0 720 282 795 846.0 0 795 283 14149 846.0 0 14149 284 15812 846.0 0 15812 285 9450 846.0 0 9450 286 680 846.0 0 680 287 68 846.0 0 68 288 683 846.0 0 683 289 75 846.0 0 75 290 165 846.0 0 165 291 65 846.0 0 65 292 79 846.0 0 79 293 96 846.0 0 96 294 132 846.0 0 132 295 104 846.0 0 104 296 723 846.0 0 723 297 41 846.0 0 41 298 51 846.0 0 51 299 45 846.0 0 45 300 21 846.0 0 21 301 35 846.0 0 35 Finished in 4.22 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 83,583 Reads with adapters: 31,062 (37.2%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 31,048 (37.1%) Total basepairs processed: 22,057,075 bp Total written (filtered): 8,435,512 bp (38.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31062 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 9 0.0 3 0 0 7 2 19 88 0.0 3 0 79 4 2 3 20 30735 0.0 4 29095 1457 126 45 12 21 88 0.0 4 17 63 5 3 22 7 0.0 4 2 1 1 1 2 23 18 0.0 4 0 0 0 13 5 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 1 28 2 0.0 4 2 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 1 53 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 87 0.0 4 0 0 0 0 87 131 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.07 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 31,048 Reads with adapters: 5,554 (17.9%) Reads written (passing filters): 31,048 (100.0%) Total basepairs processed: 8,435,512 bp Total written (filtered): 7,442,974 bp (88.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5554 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 66.6% G: 31.3% T: 0.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 485.1 0 2 4 2 121.3 0 2 6 2 7.6 0 2 9 467 0.1 0 45 422 10 635 0.0 1 27 608 11 690 0.0 1 4 686 12 3 0.0 1 0 3 13 2 0.0 1 0 2 35 1 0.0 1 1 114 1 0.0 1 1 150 12 0.0 1 7 5 199 1 0.0 1 1 208 4 0.0 1 3 1 223 1 0.0 1 1 254 2 0.0 1 2 255 4 0.0 1 4 256 546 0.0 1 525 21 257 7 0.0 1 7 258 2 0.0 1 2 259 6 0.0 1 6 260 12 0.0 1 12 261 3082 0.0 1 2916 166 262 30 0.0 1 28 2 263 17 0.0 1 17 264 22 0.0 1 22 270 1 0.0 1 1