This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 87.49 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 365,945 (10.6%) Reads that were too short: 329,700 (9.5%) Reads written (passing filters): 36,245 (1.0%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 5,414,331 bp (0.5%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 365945 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 77 846.0 0 77 7 119 846.0 0 119 8 98 846.0 0 98 9 3809 846.0 0 3809 10 41 846.0 0 41 11 28 846.0 0 28 12 11 846.0 0 11 13 27 846.0 0 27 14 29 846.0 0 29 15 12 846.0 0 12 16 22 846.0 0 22 17 29 846.0 0 29 18 31 846.0 0 31 19 28 846.0 0 28 20 23 846.0 0 23 21 42 846.0 0 42 22 20 846.0 0 20 23 20 846.0 0 20 24 31 846.0 0 31 25 35 846.0 0 35 26 18 846.0 0 18 27 17 846.0 0 17 28 28 846.0 0 28 29 14 846.0 0 14 30 10 846.0 0 10 31 27 846.0 0 27 32 29 846.0 0 29 33 97 846.0 0 97 34 56 846.0 0 56 35 34 846.0 0 34 36 25 846.0 0 25 37 59 846.0 0 59 38 498 846.0 0 498 39 26 846.0 0 26 40 14 846.0 0 14 41 23 846.0 0 23 42 26 846.0 0 26 43 38 846.0 0 38 44 21 846.0 0 21 45 47 846.0 0 47 46 20 846.0 0 20 47 25 846.0 0 25 48 27 846.0 0 27 49 10 846.0 0 10 50 13 846.0 0 13 51 18 846.0 0 18 52 27 846.0 0 27 53 37 846.0 0 37 54 14 846.0 0 14 55 26 846.0 0 26 56 34 846.0 0 34 57 38 846.0 0 38 58 60 846.0 0 60 59 38 846.0 0 38 60 259 846.0 0 259 61 82 846.0 0 82 62 26 846.0 0 26 63 20 846.0 0 20 64 13 846.0 0 13 65 46 846.0 0 46 66 25 846.0 0 25 67 25 846.0 0 25 68 20 846.0 0 20 69 17 846.0 0 17 70 62 846.0 0 62 71 31 846.0 0 31 72 29 846.0 0 29 73 33 846.0 0 33 74 20 846.0 0 20 75 130 846.0 0 130 76 76 846.0 0 76 77 95 846.0 0 95 78 30 846.0 0 30 79 28 846.0 0 28 80 27 846.0 0 27 81 16 846.0 0 16 82 17 846.0 0 17 83 10 846.0 0 10 84 15 846.0 0 15 85 23 846.0 0 23 86 22 846.0 0 22 87 25 846.0 0 25 88 21 846.0 0 21 89 19 846.0 0 19 90 24 846.0 0 24 91 22 846.0 0 22 92 22 846.0 0 22 93 19 846.0 0 19 94 20 846.0 0 20 95 31 846.0 0 31 96 24 846.0 0 24 97 16 846.0 0 16 98 31 846.0 0 31 99 17 846.0 0 17 100 42 846.0 0 42 101 23 846.0 0 23 102 22 846.0 0 22 103 28 846.0 0 28 104 36 846.0 0 36 105 18 846.0 0 18 106 26 846.0 0 26 107 20 846.0 0 20 108 12 846.0 0 12 109 15 846.0 0 15 110 19 846.0 0 19 111 21 846.0 0 21 112 41 846.0 0 41 113 175 846.0 0 175 114 391 846.0 0 391 115 493 846.0 0 493 116 14 846.0 0 14 117 29 846.0 0 29 118 15 846.0 0 15 119 18 846.0 0 18 120 19 846.0 0 19 121 20 846.0 0 20 122 26 846.0 0 26 123 22 846.0 0 22 124 20 846.0 0 20 125 19 846.0 0 19 126 27 846.0 0 27 127 20 846.0 0 20 128 22 846.0 0 22 129 29 846.0 0 29 130 26 846.0 0 26 131 22 846.0 0 22 132 15 846.0 0 15 133 26 846.0 0 26 134 43 846.0 0 43 135 32 846.0 0 32 136 31 846.0 0 31 137 28 846.0 0 28 138 96 846.0 0 96 139 135 846.0 0 135 140 82 846.0 0 82 141 23 846.0 0 23 142 206 846.0 0 206 143 167 846.0 0 167 144 176 846.0 0 176 145 24 846.0 0 24 146 50 846.0 0 50 147 298 846.0 0 298 148 568 846.0 0 568 149 994 846.0 0 994 150 82 846.0 0 82 151 23 846.0 0 23 152 17 846.0 0 17 153 24 846.0 0 24 154 18 846.0 0 18 155 18 846.0 0 18 156 20 846.0 0 20 157 17 846.0 0 17 158 20 846.0 0 20 159 13 846.0 0 13 160 16 846.0 0 16 161 24 846.0 0 24 162 33 846.0 0 33 163 31 846.0 0 31 164 27 846.0 0 27 165 29 846.0 0 29 166 14 846.0 0 14 167 23 846.0 0 23 168 44 846.0 0 44 169 24 846.0 0 24 170 52 846.0 0 52 171 30 846.0 0 30 172 17 846.0 0 17 173 29 846.0 0 29 174 34 846.0 0 34 175 22 846.0 0 22 176 27 846.0 0 27 177 26 846.0 0 26 178 38 846.0 0 38 179 90 846.0 0 90 180 55 846.0 0 55 181 187 846.0 0 187 182 98 846.0 0 98 183 52 846.0 0 52 184 78 846.0 0 78 185 149 846.0 0 149 186 142 846.0 0 142 187 226 846.0 0 226 188 224 846.0 0 224 189 1026 846.0 0 1026 190 5158 846.0 0 5158 191 1567 846.0 0 1567 192 1386 846.0 0 1386 193 577 846.0 0 577 194 486 846.0 0 486 195 915 846.0 0 915 196 1266 846.0 0 1266 197 778 846.0 0 778 198 844 846.0 0 844 199 2026 846.0 0 2026 200 2730 846.0 0 2730 201 3155 846.0 0 3155 202 6238 846.0 0 6238 203 1957 846.0 0 1957 204 3433 846.0 0 3433 205 92827 846.0 0 92827 206 18638 846.0 0 18638 207 58642 846.0 0 58642 208 17927 846.0 0 17927 209 5714 846.0 0 5714 210 17607 846.0 0 17607 211 919 846.0 0 919 212 1176 846.0 0 1176 213 4841 846.0 0 4841 214 297 846.0 0 297 215 285 846.0 0 285 216 399 846.0 0 399 217 381 846.0 0 381 218 737 846.0 0 737 219 1881 846.0 0 1881 220 2452 846.0 0 2452 221 2705 846.0 0 2705 222 2061 846.0 0 2061 223 604 846.0 0 604 224 1280 846.0 0 1280 225 2726 846.0 0 2726 226 9228 846.0 0 9228 227 14319 846.0 0 14319 228 20750 846.0 0 20750 229 618 846.0 0 618 230 217 846.0 0 217 231 132 846.0 0 132 232 22 846.0 0 22 233 10 846.0 0 10 234 21 846.0 0 21 235 29 846.0 0 29 236 124 846.0 0 124 237 11 846.0 0 11 238 40 846.0 0 40 239 16 846.0 0 16 240 27 846.0 0 27 241 20 846.0 0 20 242 24 846.0 0 24 243 20 846.0 0 20 244 102 846.0 0 102 245 52 846.0 0 52 246 95 846.0 0 95 247 269 846.0 0 269 248 166 846.0 0 166 249 252 846.0 0 252 250 44 846.0 0 44 251 73 846.0 0 73 252 147 846.0 0 147 253 176 846.0 0 176 254 97 846.0 0 97 255 132 846.0 0 132 256 139 846.0 0 139 257 181 846.0 0 181 258 150 846.0 0 150 259 273 846.0 0 273 260 359 846.0 0 359 261 400 846.0 0 400 262 8143 846.0 0 8143 263 6629 846.0 0 6629 264 19476 846.0 0 19476 265 116 846.0 0 116 266 19 846.0 0 19 267 7 846.0 0 7 268 12 846.0 0 12 269 13 846.0 0 13 270 6 846.0 0 6 271 8 846.0 0 8 272 4 846.0 0 4 273 9 846.0 0 9 274 85 846.0 0 85 275 22 846.0 0 22 276 19 846.0 0 19 277 5 846.0 0 5 278 9 846.0 0 9 279 5 846.0 0 5 280 4 846.0 0 4 281 9 846.0 0 9 282 9 846.0 0 9 283 14 846.0 0 14 284 15 846.0 0 15 285 12 846.0 0 12 286 23 846.0 0 23 287 8 846.0 0 8 288 11 846.0 0 11 289 12 846.0 0 12 290 29 846.0 0 29 291 20 846.0 0 20 292 81 846.0 0 81 293 20 846.0 0 20 294 28 846.0 0 28 295 32 846.0 0 32 296 254 846.0 0 254 297 7 846.0 0 7 298 20 846.0 0 20 299 9 846.0 0 9 300 11 846.0 0 11 301 23 846.0 0 23 Finished in 1.15 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 36,245 Reads with adapters: 3,963 (10.9%) Reads that were too short: 36 (0.1%) Reads written (passing filters): 3,927 (10.8%) Total basepairs processed: 5,414,331 bp Total written (filtered): 1,077,783 bp (19.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3963 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 3 1 16 56 0.0 3 0 56 17 3805 0.0 3 3737 61 6 1 18 23 0.0 3 1 7 12 3 19 20 0.0 3 0 1 1 18 20 2 0.0 3 0 0 0 2 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 1 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 78 16 0.0 3 0 0 1 15 80 5 0.0 3 0 0 0 5 81 5 0.0 3 0 0 0 5 84 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 3,927 Reads with adapters: 255 (6.5%) Reads written (passing filters): 3,927 (100.0%) Total basepairs processed: 1,077,783 bp Total written (filtered): 1,077,018 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 255 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.9% C: 2.7% G: 42.0% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 255 61.4 0 255