This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 88.79 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 83,893 (2.4%) Reads that were too short: 24,092 (0.7%) Reads written (passing filters): 59,801 (1.7%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 12,943,839 bp (1.2%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 83893 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 840 846.0 0 840 7 310 846.0 0 310 8 15427 846.0 0 15427 9 13297 846.0 0 13297 10 54 846.0 0 54 11 9 846.0 0 9 12 9 846.0 0 9 13 11 846.0 0 11 14 14 846.0 0 14 15 19 846.0 0 19 16 4 846.0 0 4 17 4 846.0 0 4 18 12 846.0 0 12 19 93 846.0 0 93 20 170 846.0 0 170 21 5 846.0 0 5 22 9 846.0 0 9 23 9 846.0 0 9 24 3 846.0 0 3 25 7 846.0 0 7 26 20 846.0 0 20 27 4 846.0 0 4 28 4 846.0 0 4 29 8 846.0 0 8 30 7 846.0 0 7 31 5 846.0 0 5 32 2 846.0 0 2 33 2 846.0 0 2 34 7 846.0 0 7 35 5 846.0 0 5 36 1 846.0 0 1 37 7 846.0 0 7 38 1 846.0 0 1 39 6 846.0 0 6 40 5 846.0 0 5 41 5 846.0 0 5 42 2 846.0 0 2 43 72 846.0 0 72 44 46 846.0 0 46 45 97 846.0 0 97 46 14 846.0 0 14 47 7 846.0 0 7 48 9 846.0 0 9 49 7 846.0 0 7 50 5 846.0 0 5 51 5 846.0 0 5 52 8 846.0 0 8 53 5 846.0 0 5 54 4 846.0 0 4 55 12 846.0 0 12 56 10 846.0 0 10 57 7 846.0 0 7 58 7 846.0 0 7 59 10 846.0 0 10 60 24 846.0 0 24 61 8 846.0 0 8 62 9 846.0 0 9 63 11 846.0 0 11 64 2 846.0 0 2 65 11 846.0 0 11 66 5 846.0 0 5 67 9 846.0 0 9 68 12 846.0 0 12 69 7 846.0 0 7 70 8 846.0 0 8 71 10 846.0 0 10 72 24 846.0 0 24 73 31 846.0 0 31 74 63 846.0 0 63 75 76 846.0 0 76 76 181 846.0 0 181 77 2194 846.0 0 2194 78 593 846.0 0 593 79 629 846.0 0 629 80 17 846.0 0 17 81 14 846.0 0 14 82 32 846.0 0 32 83 39 846.0 0 39 84 109 846.0 0 109 85 62 846.0 0 62 86 31 846.0 0 31 87 12 846.0 0 12 88 18 846.0 0 18 89 28 846.0 0 28 90 32 846.0 0 32 91 82 846.0 0 82 92 89 846.0 0 89 93 78 846.0 0 78 94 69 846.0 0 69 95 123 846.0 0 123 96 49 846.0 0 49 97 35 846.0 0 35 98 40 846.0 0 40 99 23 846.0 0 23 100 10 846.0 0 10 101 12 846.0 0 12 102 22 846.0 0 22 103 6 846.0 0 6 104 2 846.0 0 2 105 5 846.0 0 5 106 19 846.0 0 19 107 18 846.0 0 18 108 19 846.0 0 19 109 14 846.0 0 14 110 14 846.0 0 14 111 14 846.0 0 14 112 31 846.0 0 31 113 38 846.0 0 38 114 79 846.0 0 79 115 95 846.0 0 95 116 63 846.0 0 63 117 40 846.0 0 40 118 30 846.0 0 30 119 26 846.0 0 26 120 23 846.0 0 23 121 29 846.0 0 29 122 43 846.0 0 43 123 54 846.0 0 54 124 44 846.0 0 44 125 42 846.0 0 42 126 63 846.0 0 63 127 42 846.0 0 42 128 68 846.0 0 68 129 108 846.0 0 108 130 32 846.0 0 32 131 35 846.0 0 35 132 21 846.0 0 21 133 22 846.0 0 22 134 61 846.0 0 61 135 178 846.0 0 178 136 244 846.0 0 244 137 119 846.0 0 119 138 55 846.0 0 55 139 166 846.0 0 166 140 97 846.0 0 97 141 83 846.0 0 83 142 292 846.0 0 292 143 67 846.0 0 67 144 27 846.0 0 27 145 28 846.0 0 28 146 45 846.0 0 45 147 29 846.0 0 29 148 80 846.0 0 80 149 46 846.0 0 46 150 59 846.0 0 59 151 29 846.0 0 29 152 28 846.0 0 28 153 44 846.0 0 44 154 47 846.0 0 47 155 57 846.0 0 57 156 46 846.0 0 46 157 38 846.0 0 38 158 167 846.0 0 167 159 31 846.0 0 31 160 25 846.0 0 25 161 32 846.0 0 32 162 58 846.0 0 58 163 62 846.0 0 62 164 90 846.0 0 90 165 59 846.0 0 59 166 67 846.0 0 67 167 58 846.0 0 58 168 51 846.0 0 51 169 39 846.0 0 39 170 42 846.0 0 42 171 55 846.0 0 55 172 117 846.0 0 117 173 270 846.0 0 270 174 289 846.0 0 289 175 692 846.0 0 692 176 82 846.0 0 82 177 67 846.0 0 67 178 89 846.0 0 89 179 80 846.0 0 80 180 321 846.0 0 321 181 932 846.0 0 932 182 262 846.0 0 262 183 59 846.0 0 59 184 80 846.0 0 80 185 58 846.0 0 58 186 62 846.0 0 62 187 91 846.0 0 91 188 157 846.0 0 157 189 213 846.0 0 213 190 351 846.0 0 351 191 2825 846.0 0 2825 192 9908 846.0 0 9908 193 2637 846.0 0 2637 194 113 846.0 0 113 195 51 846.0 0 51 196 59 846.0 0 59 197 80 846.0 0 80 198 47 846.0 0 47 199 47 846.0 0 47 200 75 846.0 0 75 201 92 846.0 0 92 202 332 846.0 0 332 203 267 846.0 0 267 204 203 846.0 0 203 205 151 846.0 0 151 206 29 846.0 0 29 207 60 846.0 0 60 208 212 846.0 0 212 209 139 846.0 0 139 210 101 846.0 0 101 211 58 846.0 0 58 212 29 846.0 0 29 213 43 846.0 0 43 214 29 846.0 0 29 215 47 846.0 0 47 216 52 846.0 0 52 217 43 846.0 0 43 218 32 846.0 0 32 219 20 846.0 0 20 220 30 846.0 0 30 221 29 846.0 0 29 222 23 846.0 0 23 223 25 846.0 0 25 224 15 846.0 0 15 225 39 846.0 0 39 226 43 846.0 0 43 227 81 846.0 0 81 228 27 846.0 0 27 229 43 846.0 0 43 230 39 846.0 0 39 231 30 846.0 0 30 232 50 846.0 0 50 233 30 846.0 0 30 234 52 846.0 0 52 235 40 846.0 0 40 236 53 846.0 0 53 237 62 846.0 0 62 238 23 846.0 0 23 239 20 846.0 0 20 240 27 846.0 0 27 241 27 846.0 0 27 242 21 846.0 0 21 243 21 846.0 0 21 244 31 846.0 0 31 245 29 846.0 0 29 246 24 846.0 0 24 247 43 846.0 0 43 248 80 846.0 0 80 249 101 846.0 0 101 250 177 846.0 0 177 251 126 846.0 0 126 252 142 846.0 0 142 253 103 846.0 0 103 254 118 846.0 0 118 255 68 846.0 0 68 256 46 846.0 0 46 257 52 846.0 0 52 258 501 846.0 0 501 259 1357 846.0 0 1357 260 969 846.0 0 969 261 1732 846.0 0 1732 262 2042 846.0 0 2042 263 567 846.0 0 567 264 1133 846.0 0 1133 265 263 846.0 0 263 266 695 846.0 0 695 267 30 846.0 0 30 268 68 846.0 0 68 269 147 846.0 0 147 270 1009 846.0 0 1009 271 114 846.0 0 114 272 110 846.0 0 110 273 48 846.0 0 48 274 66 846.0 0 66 275 212 846.0 0 212 276 260 846.0 0 260 277 222 846.0 0 222 278 41 846.0 0 41 279 55 846.0 0 55 280 78 846.0 0 78 281 169 846.0 0 169 282 142 846.0 0 142 283 463 846.0 0 463 284 1191 846.0 0 1191 285 752 846.0 0 752 286 1782 846.0 0 1782 287 149 846.0 0 149 288 156 846.0 0 156 289 772 846.0 0 772 290 1074 846.0 0 1074 291 167 846.0 0 167 292 48 846.0 0 48 293 75 846.0 0 75 294 82 846.0 0 82 295 505 846.0 0 505 296 365 846.0 0 365 297 441 846.0 0 441 298 47 846.0 0 47 299 23 846.0 0 23 300 19 846.0 0 19 301 114 846.0 0 114 Finished in 2.65 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 59,801 Reads with adapters: 13,746 (23.0%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 13,723 (22.9%) Total basepairs processed: 12,943,839 bp Total written (filtered): 3,732,173 bp (28.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13746 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 294 0.0 3 0 273 11 5 5 20 13365 0.0 4 12581 700 63 15 6 21 27 0.0 4 10 15 1 0 1 22 4 0.0 4 1 0 3 23 13 0.0 4 2 0 0 8 3 26 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 58 1 0.0 4 0 0 0 1 61 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 67 6 0.0 4 0 0 0 0 6 69 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 230 2 0.0 4 0 0 0 1 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 281 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 13,723 Reads with adapters: 751 (5.5%) Reads written (passing filters): 13,723 (100.0%) Total basepairs processed: 3,732,173 bp Total written (filtered): 3,719,937 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 751 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 4.8% G: 88.3% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 214.4 0 6 4 3 53.6 0 3 9 148 0.1 0 11 137 10 409 0.0 1 16 393 11 164 0.0 1 1 163 12 1 0.0 1 0 1 122 2 0.0 1 2 208 1 0.0 1 1 257 2 0.0 1 2 261 4 0.0 1 4 263 2 0.0 1 1 1 269 2 0.0 1 2 270 3 0.0 1 3 271 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.