This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.97 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 78,890 (2.3%) Reads that were too short: 30,357 (0.9%) Reads written (passing filters): 48,533 (1.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 10,762,066 bp (1.0%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 78890 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 240 846.0 0 240 7 8827 846.0 0 8827 8 11845 846.0 0 11845 9 40 846.0 0 40 10 7 846.0 0 7 11 6 846.0 0 6 12 37 846.0 0 37 13 9 846.0 0 9 14 9 846.0 0 9 15 13 846.0 0 13 16 4 846.0 0 4 17 16 846.0 0 16 18 31 846.0 0 31 19 55 846.0 0 55 20 14 846.0 0 14 21 8 846.0 0 8 22 3 846.0 0 3 23 9 846.0 0 9 24 6 846.0 0 6 25 9 846.0 0 9 26 19 846.0 0 19 27 11 846.0 0 11 28 24 846.0 0 24 29 17 846.0 0 17 30 35 846.0 0 35 31 11 846.0 0 11 32 12 846.0 0 12 33 20 846.0 0 20 34 30 846.0 0 30 35 19 846.0 0 19 36 237 846.0 0 237 37 97 846.0 0 97 38 101 846.0 0 101 39 37 846.0 0 37 40 9 846.0 0 9 41 11 846.0 0 11 42 40 846.0 0 40 43 1035 846.0 0 1035 44 89 846.0 0 89 45 202 846.0 0 202 46 22 846.0 0 22 47 14 846.0 0 14 48 15 846.0 0 15 49 17 846.0 0 17 50 15 846.0 0 15 51 9 846.0 0 9 52 7 846.0 0 7 53 8 846.0 0 8 54 9 846.0 0 9 55 12 846.0 0 12 56 16 846.0 0 16 57 112 846.0 0 112 58 18 846.0 0 18 59 18 846.0 0 18 60 8 846.0 0 8 61 14 846.0 0 14 62 11 846.0 0 11 63 12 846.0 0 12 64 2 846.0 0 2 65 10 846.0 0 10 66 11 846.0 0 11 67 10 846.0 0 10 68 8 846.0 0 8 69 5 846.0 0 5 70 11 846.0 0 11 71 25 846.0 0 25 72 29 846.0 0 29 73 41 846.0 0 41 74 67 846.0 0 67 75 193 846.0 0 193 76 59 846.0 0 59 77 52 846.0 0 52 78 185 846.0 0 185 79 84 846.0 0 84 80 58 846.0 0 58 81 77 846.0 0 77 82 123 846.0 0 123 83 126 846.0 0 126 84 123 846.0 0 123 85 83 846.0 0 83 86 73 846.0 0 73 87 62 846.0 0 62 88 206 846.0 0 206 89 176 846.0 0 176 90 203 846.0 0 203 91 290 846.0 0 290 92 138 846.0 0 138 93 142 846.0 0 142 94 303 846.0 0 303 95 318 846.0 0 318 96 182 846.0 0 182 97 151 846.0 0 151 98 116 846.0 0 116 99 166 846.0 0 166 100 71 846.0 0 71 101 45 846.0 0 45 102 17 846.0 0 17 103 13 846.0 0 13 104 8 846.0 0 8 105 22 846.0 0 22 106 60 846.0 0 60 107 123 846.0 0 123 108 148 846.0 0 148 109 144 846.0 0 144 110 70 846.0 0 70 111 91 846.0 0 91 112 100 846.0 0 100 113 149 846.0 0 149 114 194 846.0 0 194 115 166 846.0 0 166 116 115 846.0 0 115 117 63 846.0 0 63 118 70 846.0 0 70 119 105 846.0 0 105 120 236 846.0 0 236 121 352 846.0 0 352 122 231 846.0 0 231 123 121 846.0 0 121 124 111 846.0 0 111 125 181 846.0 0 181 126 167 846.0 0 167 127 250 846.0 0 250 128 605 846.0 0 605 129 358 846.0 0 358 130 244 846.0 0 244 131 136 846.0 0 136 132 276 846.0 0 276 133 150 846.0 0 150 134 244 846.0 0 244 135 1040 846.0 0 1040 136 715 846.0 0 715 137 378 846.0 0 378 138 124 846.0 0 124 139 189 846.0 0 189 140 333 846.0 0 333 141 299 846.0 0 299 142 374 846.0 0 374 143 272 846.0 0 272 144 173 846.0 0 173 145 238 846.0 0 238 146 195 846.0 0 195 147 237 846.0 0 237 148 232 846.0 0 232 149 252 846.0 0 252 150 216 846.0 0 216 151 159 846.0 0 159 152 127 846.0 0 127 153 168 846.0 0 168 154 223 846.0 0 223 155 245 846.0 0 245 156 233 846.0 0 233 157 163 846.0 0 163 158 154 846.0 0 154 159 203 846.0 0 203 160 152 846.0 0 152 161 170 846.0 0 170 162 120 846.0 0 120 163 177 846.0 0 177 164 190 846.0 0 190 165 251 846.0 0 251 166 276 846.0 0 276 167 269 846.0 0 269 168 212 846.0 0 212 169 167 846.0 0 167 170 105 846.0 0 105 171 112 846.0 0 112 172 101 846.0 0 101 173 166 846.0 0 166 174 282 846.0 0 282 175 232 846.0 0 232 176 176 846.0 0 176 177 149 846.0 0 149 178 151 846.0 0 151 179 146 846.0 0 146 180 215 846.0 0 215 181 289 846.0 0 289 182 248 846.0 0 248 183 183 846.0 0 183 184 233 846.0 0 233 185 234 846.0 0 234 186 234 846.0 0 234 187 131 846.0 0 131 188 143 846.0 0 143 189 251 846.0 0 251 190 208 846.0 0 208 191 316 846.0 0 316 192 227 846.0 0 227 193 152 846.0 0 152 194 273 846.0 0 273 195 413 846.0 0 413 196 345 846.0 0 345 197 154 846.0 0 154 198 100 846.0 0 100 199 97 846.0 0 97 200 175 846.0 0 175 201 441 846.0 0 441 202 583 846.0 0 583 203 450 846.0 0 450 204 341 846.0 0 341 205 207 846.0 0 207 206 158 846.0 0 158 207 297 846.0 0 297 208 489 846.0 0 489 209 442 846.0 0 442 210 402 846.0 0 402 211 390 846.0 0 390 212 354 846.0 0 354 213 276 846.0 0 276 214 274 846.0 0 274 215 375 846.0 0 375 216 408 846.0 0 408 217 284 846.0 0 284 218 151 846.0 0 151 219 141 846.0 0 141 220 293 846.0 0 293 221 436 846.0 0 436 222 509 846.0 0 509 223 238 846.0 0 238 224 252 846.0 0 252 225 660 846.0 0 660 226 274 846.0 0 274 227 187 846.0 0 187 228 187 846.0 0 187 229 181 846.0 0 181 230 261 846.0 0 261 231 224 846.0 0 224 232 281 846.0 0 281 233 378 846.0 0 378 234 332 846.0 0 332 235 302 846.0 0 302 236 272 846.0 0 272 237 224 846.0 0 224 238 176 846.0 0 176 239 106 846.0 0 106 240 201 846.0 0 201 241 261 846.0 0 261 242 644 846.0 0 644 243 523 846.0 0 523 244 466 846.0 0 466 245 200 846.0 0 200 246 117 846.0 0 117 247 71 846.0 0 71 248 54 846.0 0 54 249 93 846.0 0 93 250 162 846.0 0 162 251 350 846.0 0 350 252 274 846.0 0 274 253 419 846.0 0 419 254 344 846.0 0 344 255 291 846.0 0 291 256 217 846.0 0 217 257 125 846.0 0 125 258 136 846.0 0 136 259 132 846.0 0 132 260 189 846.0 0 189 261 214 846.0 0 214 262 246 846.0 0 246 263 250 846.0 0 250 264 288 846.0 0 288 265 248 846.0 0 248 266 187 846.0 0 187 267 123 846.0 0 123 268 118 846.0 0 118 269 1146 846.0 0 1146 270 643 846.0 0 643 271 594 846.0 0 594 272 383 846.0 0 383 273 271 846.0 0 271 274 192 846.0 0 192 275 194 846.0 0 194 276 320 846.0 0 320 277 222 846.0 0 222 278 217 846.0 0 217 279 188 846.0 0 188 280 228 846.0 0 228 281 255 846.0 0 255 282 249 846.0 0 249 283 242 846.0 0 242 284 404 846.0 0 404 285 383 846.0 0 383 286 272 846.0 0 272 287 127 846.0 0 127 288 115 846.0 0 115 289 205 846.0 0 205 290 1153 846.0 0 1153 291 404 846.0 0 404 292 280 846.0 0 280 293 172 846.0 0 172 294 504 846.0 0 504 295 307 846.0 0 307 296 405 846.0 0 405 297 348 846.0 0 348 298 370 846.0 0 370 299 145 846.0 0 145 300 343 846.0 0 343 301 338 846.0 0 338 Finished in 2.13 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 48,533 Reads with adapters: 12,223 (25.2%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 12,204 (25.1%) Total basepairs processed: 10,762,066 bp Total written (filtered): 3,321,430 bp (30.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12223 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 8 0.0 3 0 0 6 1 1 19 155 0.0 3 0 152 0 2 1 20 11903 0.0 4 11616 248 29 7 3 21 30 0.0 4 3 25 1 1 22 2 0.0 4 1 0 1 23 8 0.0 4 0 0 0 7 1 40 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 92 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 1 115 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 85 0.0 4 0 0 0 0 85 132 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 246 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 12,204 Reads with adapters: 2,024 (16.6%) Reads written (passing filters): 12,204 (100.0%) Total basepairs processed: 3,321,430 bp Total written (filtered): 3,102,173 bp (93.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2024 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 31.4% C: 3.7% G: 57.0% T: 7.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 190.7 0 1 9 3 0.0 0 0 3 10 80 0.0 1 2 78 11 1145 0.0 1 4 1141 12 2 0.0 1 0 2 123 2 0.0 1 1 1 151 10 0.0 1 10 209 1 0.0 1 1 224 1 0.0 1 1 240 1 0.0 1 1 256 1 0.0 1 1 257 1 0.0 1 1 258 12 0.0 1 12 259 491 0.0 1 473 18 260 13 0.0 1 11 2 261 2 0.0 1 2 262 7 0.0 1 6 1 263 3 0.0 1 3 264 130 0.0 1 129 1 265 1 0.0 1 1 269 101 0.0 1 99 2 270 15 0.0 1 14 1 272 1 0.0 1 1