This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.60 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 95,446 (2.8%) Reads that were too short: 8,177 (0.2%) Reads written (passing filters): 87,269 (2.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 21,978,960 bp (2.1%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 95446 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 388 846.0 0 388 7 665 846.0 0 665 8 563 846.0 0 563 9 48024 846.0 0 48024 10 87 846.0 0 87 11 83 846.0 0 83 12 27 846.0 0 27 13 27 846.0 0 27 14 33 846.0 0 33 15 22 846.0 0 22 16 138 846.0 0 138 17 51 846.0 0 51 18 61 846.0 0 61 19 15 846.0 0 15 20 25 846.0 0 25 21 22 846.0 0 22 22 21 846.0 0 21 23 29 846.0 0 29 24 19 846.0 0 19 25 27 846.0 0 27 26 22 846.0 0 22 27 20 846.0 0 20 28 31 846.0 0 31 29 40 846.0 0 40 30 20 846.0 0 20 31 22 846.0 0 22 32 31 846.0 0 31 33 303 846.0 0 303 34 26 846.0 0 26 35 31 846.0 0 31 36 23 846.0 0 23 37 27 846.0 0 27 38 27 846.0 0 27 39 23 846.0 0 23 40 23 846.0 0 23 41 22 846.0 0 22 42 28 846.0 0 28 43 31 846.0 0 31 44 29 846.0 0 29 45 24 846.0 0 24 46 66 846.0 0 66 47 93 846.0 0 93 48 67 846.0 0 67 49 32 846.0 0 32 50 20 846.0 0 20 51 19 846.0 0 19 52 38 846.0 0 38 53 22 846.0 0 22 54 22 846.0 0 22 55 27 846.0 0 27 56 21 846.0 0 21 57 31 846.0 0 31 58 35 846.0 0 35 59 26 846.0 0 26 60 21 846.0 0 21 61 22 846.0 0 22 62 34 846.0 0 34 63 29 846.0 0 29 64 15 846.0 0 15 65 22 846.0 0 22 66 35 846.0 0 35 67 32 846.0 0 32 68 34 846.0 0 34 69 36 846.0 0 36 70 42 846.0 0 42 71 32 846.0 0 32 72 35 846.0 0 35 73 30 846.0 0 30 74 31 846.0 0 31 75 20 846.0 0 20 76 60 846.0 0 60 77 43 846.0 0 43 78 53 846.0 0 53 79 104 846.0 0 104 80 150 846.0 0 150 81 372 846.0 0 372 82 126 846.0 0 126 83 92 846.0 0 92 84 148 846.0 0 148 85 110 846.0 0 110 86 91 846.0 0 91 87 127 846.0 0 127 88 119 846.0 0 119 89 138 846.0 0 138 90 112 846.0 0 112 91 140 846.0 0 140 92 229 846.0 0 229 93 344 846.0 0 344 94 7314 846.0 0 7314 95 8033 846.0 0 8033 96 8476 846.0 0 8476 97 209 846.0 0 209 98 329 846.0 0 329 99 62 846.0 0 62 100 68 846.0 0 68 101 85 846.0 0 85 102 48 846.0 0 48 103 34 846.0 0 34 104 24 846.0 0 24 105 32 846.0 0 32 106 25 846.0 0 25 107 36 846.0 0 36 108 29 846.0 0 29 109 66 846.0 0 66 110 449 846.0 0 449 111 215 846.0 0 215 112 197 846.0 0 197 113 52 846.0 0 52 114 36 846.0 0 36 115 71 846.0 0 71 116 62 846.0 0 62 117 73 846.0 0 73 118 72 846.0 0 72 119 89 846.0 0 89 120 57 846.0 0 57 121 41 846.0 0 41 122 51 846.0 0 51 123 30 846.0 0 30 124 64 846.0 0 64 125 67 846.0 0 67 126 83 846.0 0 83 127 53 846.0 0 53 128 146 846.0 0 146 129 149 846.0 0 149 130 89 846.0 0 89 131 302 846.0 0 302 132 166 846.0 0 166 133 91 846.0 0 91 134 55 846.0 0 55 135 67 846.0 0 67 136 86 846.0 0 86 137 153 846.0 0 153 138 205 846.0 0 205 139 196 846.0 0 196 140 59 846.0 0 59 141 88 846.0 0 88 142 90 846.0 0 90 143 155 846.0 0 155 144 117 846.0 0 117 145 116 846.0 0 116 146 103 846.0 0 103 147 46 846.0 0 46 148 50 846.0 0 50 149 54 846.0 0 54 150 42 846.0 0 42 151 45 846.0 0 45 152 34 846.0 0 34 153 29 846.0 0 29 154 33 846.0 0 33 155 55 846.0 0 55 156 54 846.0 0 54 157 56 846.0 0 56 158 79 846.0 0 79 159 78 846.0 0 78 160 139 846.0 0 139 161 37 846.0 0 37 162 29 846.0 0 29 163 29 846.0 0 29 164 46 846.0 0 46 165 61 846.0 0 61 166 57 846.0 0 57 167 60 846.0 0 60 168 50 846.0 0 50 169 49 846.0 0 49 170 53 846.0 0 53 171 37 846.0 0 37 172 66 846.0 0 66 173 51 846.0 0 51 174 54 846.0 0 54 175 58 846.0 0 58 176 65 846.0 0 65 177 51 846.0 0 51 178 45 846.0 0 45 179 62 846.0 0 62 180 84 846.0 0 84 181 66 846.0 0 66 182 55 846.0 0 55 183 66 846.0 0 66 184 65 846.0 0 65 185 65 846.0 0 65 186 115 846.0 0 115 187 195 846.0 0 195 188 56 846.0 0 56 189 34 846.0 0 34 190 39 846.0 0 39 191 83 846.0 0 83 192 157 846.0 0 157 193 114 846.0 0 114 194 234 846.0 0 234 195 65 846.0 0 65 196 36 846.0 0 36 197 38 846.0 0 38 198 69 846.0 0 69 199 74 846.0 0 74 200 38 846.0 0 38 201 25 846.0 0 25 202 28 846.0 0 28 203 74 846.0 0 74 204 90 846.0 0 90 205 242 846.0 0 242 206 320 846.0 0 320 207 142 846.0 0 142 208 56 846.0 0 56 209 60 846.0 0 60 210 63 846.0 0 63 211 76 846.0 0 76 212 87 846.0 0 87 213 94 846.0 0 94 214 69 846.0 0 69 215 58 846.0 0 58 216 65 846.0 0 65 217 67 846.0 0 67 218 85 846.0 0 85 219 75 846.0 0 75 220 65 846.0 0 65 221 48 846.0 0 48 222 38 846.0 0 38 223 55 846.0 0 55 224 76 846.0 0 76 225 61 846.0 0 61 226 65 846.0 0 65 227 93 846.0 0 93 228 77 846.0 0 77 229 86 846.0 0 86 230 48 846.0 0 48 231 63 846.0 0 63 232 64 846.0 0 64 233 67 846.0 0 67 234 85 846.0 0 85 235 61 846.0 0 61 236 117 846.0 0 117 237 101 846.0 0 101 238 130 846.0 0 130 239 136 846.0 0 136 240 107 846.0 0 107 241 42 846.0 0 42 242 41 846.0 0 41 243 51 846.0 0 51 244 55 846.0 0 55 245 62 846.0 0 62 246 49 846.0 0 49 247 86 846.0 0 86 248 48 846.0 0 48 249 33 846.0 0 33 250 22 846.0 0 22 251 20 846.0 0 20 252 69 846.0 0 69 253 78 846.0 0 78 254 63 846.0 0 63 255 78 846.0 0 78 256 79 846.0 0 79 257 111 846.0 0 111 258 75 846.0 0 75 259 59 846.0 0 59 260 36 846.0 0 36 261 75 846.0 0 75 262 90 846.0 0 90 263 76 846.0 0 76 264 67 846.0 0 67 265 58 846.0 0 58 266 77 846.0 0 77 267 105 846.0 0 105 268 83 846.0 0 83 269 40 846.0 0 40 270 25 846.0 0 25 271 96 846.0 0 96 272 657 846.0 0 657 273 119 846.0 0 119 274 69 846.0 0 69 275 45 846.0 0 45 276 65 846.0 0 65 277 34 846.0 0 34 278 66 846.0 0 66 279 38 846.0 0 38 280 83 846.0 0 83 281 46 846.0 0 46 282 47 846.0 0 47 283 66 846.0 0 66 284 82 846.0 0 82 285 66 846.0 0 66 286 126 846.0 0 126 287 102 846.0 0 102 288 121 846.0 0 121 289 50 846.0 0 50 290 47 846.0 0 47 291 76 846.0 0 76 292 138 846.0 0 138 293 102 846.0 0 102 294 49 846.0 0 49 295 45 846.0 0 45 296 31 846.0 0 31 297 56 846.0 0 56 298 146 846.0 0 146 299 106 846.0 0 106 300 121 846.0 0 121 301 35 846.0 0 35 Finished in 3.75 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 87,269 Reads with adapters: 49,368 (56.6%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 49,358 (56.6%) Total basepairs processed: 21,978,960 bp Total written (filtered): 13,573,995 bp (61.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49368 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 18 0.0 3 0 0 17 1 16 386 0.0 3 0 379 5 2 17 48676 0.0 3 47831 829 13 3 18 100 0.0 3 3 91 6 19 34 0.0 3 0 0 20 14 20 128 0.0 3 106 3 0 19 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.75 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 49,358 Reads with adapters: 3,211 (6.5%) Reads written (passing filters): 49,358 (100.0%) Total basepairs processed: 13,573,995 bp Total written (filtered): 13,564,353 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3211 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.4% C: 8.3% G: 26.7% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3207 771.2 0 3207 4 2 192.8 0 2 6 1 12.1 0 1 7 1 3.0 0 1