This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.94 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 246,093 (7.1%) Reads that were too short: 8,923 (0.3%) Reads written (passing filters): 237,170 (6.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 42,904,252 bp (4.1%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 246093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 110 846.0 0 110 7 7101 846.0 0 7101 8 16 846.0 0 16 9 18 846.0 0 18 10 19 846.0 0 19 11 12 846.0 0 12 12 12 846.0 0 12 13 21 846.0 0 21 14 16 846.0 0 16 15 18 846.0 0 18 16 16 846.0 0 16 17 20 846.0 0 20 18 16 846.0 0 16 19 22 846.0 0 22 20 16 846.0 0 16 21 35 846.0 0 35 22 20 846.0 0 20 23 19 846.0 0 19 24 15 846.0 0 15 25 24 846.0 0 24 26 23 846.0 0 23 27 17 846.0 0 17 28 15 846.0 0 15 29 19 846.0 0 19 30 24 846.0 0 24 31 14 846.0 0 14 32 14 846.0 0 14 33 26 846.0 0 26 34 12 846.0 0 12 35 15 846.0 0 15 36 16 846.0 0 16 37 26 846.0 0 26 38 30 846.0 0 30 39 31 846.0 0 31 40 14 846.0 0 14 41 19 846.0 0 19 42 27 846.0 0 27 43 20 846.0 0 20 44 16 846.0 0 16 45 24 846.0 0 24 46 35 846.0 0 35 47 16 846.0 0 16 48 28 846.0 0 28 49 32 846.0 0 32 50 16 846.0 0 16 51 31 846.0 0 31 52 26 846.0 0 26 53 25 846.0 0 25 54 39 846.0 0 39 55 27 846.0 0 27 56 54 846.0 0 54 57 30 846.0 0 30 58 40 846.0 0 40 59 520 846.0 0 520 60 185 846.0 0 185 61 20556 846.0 0 20556 62 61 846.0 0 61 63 44 846.0 0 44 64 30 846.0 0 30 65 17 846.0 0 17 66 26 846.0 0 26 67 28 846.0 0 28 68 26 846.0 0 26 69 25 846.0 0 25 70 18 846.0 0 18 71 21 846.0 0 21 72 26 846.0 0 26 73 20 846.0 0 20 74 22 846.0 0 22 75 19 846.0 0 19 76 32 846.0 0 32 77 115 846.0 0 115 78 46 846.0 0 46 79 35 846.0 0 35 80 24 846.0 0 24 81 25 846.0 0 25 82 24 846.0 0 24 83 43 846.0 0 43 84 130 846.0 0 130 85 45 846.0 0 45 86 41 846.0 0 41 87 109 846.0 0 109 88 24 846.0 0 24 89 25 846.0 0 25 90 33 846.0 0 33 91 30 846.0 0 30 92 100 846.0 0 100 93 191 846.0 0 191 94 234 846.0 0 234 95 49 846.0 0 49 96 73 846.0 0 73 97 224 846.0 0 224 98 61 846.0 0 61 99 92 846.0 0 92 100 87 846.0 0 87 101 109 846.0 0 109 102 222 846.0 0 222 103 103 846.0 0 103 104 68 846.0 0 68 105 40 846.0 0 40 106 88 846.0 0 88 107 147 846.0 0 147 108 231 846.0 0 231 109 266 846.0 0 266 110 495 846.0 0 495 111 705 846.0 0 705 112 891 846.0 0 891 113 1530 846.0 0 1530 114 58535 846.0 0 58535 115 19953 846.0 0 19953 116 30375 846.0 0 30375 117 272 846.0 0 272 118 110 846.0 0 110 119 56 846.0 0 56 120 52 846.0 0 52 121 33 846.0 0 33 122 27 846.0 0 27 123 37 846.0 0 37 124 46 846.0 0 46 125 44 846.0 0 44 126 118 846.0 0 118 127 95 846.0 0 95 128 354 846.0 0 354 129 192 846.0 0 192 130 351 846.0 0 351 131 70 846.0 0 70 132 56 846.0 0 56 133 49 846.0 0 49 134 54 846.0 0 54 135 94 846.0 0 94 136 124 846.0 0 124 137 182 846.0 0 182 138 89 846.0 0 89 139 41 846.0 0 41 140 150 846.0 0 150 141 397 846.0 0 397 142 378 846.0 0 378 143 267 846.0 0 267 144 205 846.0 0 205 145 340 846.0 0 340 146 625 846.0 0 625 147 21161 846.0 0 21161 148 1373 846.0 0 1373 149 9005 846.0 0 9005 150 512 846.0 0 512 151 396 846.0 0 396 152 462 846.0 0 462 153 5471 846.0 0 5471 154 42120 846.0 0 42120 155 2707 846.0 0 2707 156 226 846.0 0 226 157 128 846.0 0 128 158 92 846.0 0 92 159 31 846.0 0 31 160 43 846.0 0 43 161 52 846.0 0 52 162 87 846.0 0 87 163 94 846.0 0 94 164 64 846.0 0 64 165 35 846.0 0 35 166 46 846.0 0 46 167 45 846.0 0 45 168 109 846.0 0 109 169 311 846.0 0 311 170 137 846.0 0 137 171 105 846.0 0 105 172 25 846.0 0 25 173 30 846.0 0 30 174 35 846.0 0 35 175 63 846.0 0 63 176 53 846.0 0 53 177 31 846.0 0 31 178 43 846.0 0 43 179 37 846.0 0 37 180 38 846.0 0 38 181 56 846.0 0 56 182 105 846.0 0 105 183 385 846.0 0 385 184 106 846.0 0 106 185 126 846.0 0 126 186 83 846.0 0 83 187 57 846.0 0 57 188 33 846.0 0 33 189 48 846.0 0 48 190 53 846.0 0 53 191 43 846.0 0 43 192 41 846.0 0 41 193 39 846.0 0 39 194 38 846.0 0 38 195 31 846.0 0 31 196 33 846.0 0 33 197 38 846.0 0 38 198 42 846.0 0 42 199 27 846.0 0 27 200 23 846.0 0 23 201 47 846.0 0 47 202 435 846.0 0 435 203 218 846.0 0 218 204 255 846.0 0 255 205 54 846.0 0 54 206 36 846.0 0 36 207 23 846.0 0 23 208 19 846.0 0 19 209 31 846.0 0 31 210 24 846.0 0 24 211 45 846.0 0 45 212 35 846.0 0 35 213 37 846.0 0 37 214 41 846.0 0 41 215 56 846.0 0 56 216 93 846.0 0 93 217 117 846.0 0 117 218 94 846.0 0 94 219 24 846.0 0 24 220 34 846.0 0 34 221 35 846.0 0 35 222 41 846.0 0 41 223 33 846.0 0 33 224 73 846.0 0 73 225 46 846.0 0 46 226 111 846.0 0 111 227 68 846.0 0 68 228 128 846.0 0 128 229 21 846.0 0 21 230 44 846.0 0 44 231 17 846.0 0 17 232 33 846.0 0 33 233 51 846.0 0 51 234 59 846.0 0 59 235 76 846.0 0 76 236 101 846.0 0 101 237 68 846.0 0 68 238 53 846.0 0 53 239 27 846.0 0 27 240 28 846.0 0 28 241 32 846.0 0 32 242 30 846.0 0 30 243 25 846.0 0 25 244 47 846.0 0 47 245 52 846.0 0 52 246 58 846.0 0 58 247 26 846.0 0 26 248 23 846.0 0 23 249 114 846.0 0 114 250 25 846.0 0 25 251 42 846.0 0 42 252 32 846.0 0 32 253 50 846.0 0 50 254 101 846.0 0 101 255 62 846.0 0 62 256 71 846.0 0 71 257 39 846.0 0 39 258 33 846.0 0 33 259 54 846.0 0 54 260 53 846.0 0 53 261 50 846.0 0 50 262 43 846.0 0 43 263 61 846.0 0 61 264 44 846.0 0 44 265 45 846.0 0 45 266 56 846.0 0 56 267 58 846.0 0 58 268 29 846.0 0 29 269 29 846.0 0 29 270 52 846.0 0 52 271 62 846.0 0 62 272 77 846.0 0 77 273 235 846.0 0 235 274 235 846.0 0 235 275 306 846.0 0 306 276 101 846.0 0 101 277 60 846.0 0 60 278 45 846.0 0 45 279 105 846.0 0 105 280 221 846.0 0 221 281 694 846.0 0 694 282 223 846.0 0 223 283 228 846.0 0 228 284 103 846.0 0 103 285 100 846.0 0 100 286 61 846.0 0 61 287 51 846.0 0 51 288 42 846.0 0 42 289 49 846.0 0 49 290 163 846.0 0 163 291 946 846.0 0 946 292 231 846.0 0 231 293 109 846.0 0 109 294 70 846.0 0 70 295 45 846.0 0 45 296 32 846.0 0 32 297 23 846.0 0 23 298 29 846.0 0 29 299 21 846.0 0 21 300 22 846.0 0 22 301 14 846.0 0 14 Finished in 8.00 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 237,170 Reads with adapters: 7,224 (3.0%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 7,175 (3.0%) Total basepairs processed: 42,904,252 bp Total written (filtered): 1,981,517 bp (4.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7224 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 70 0.0 3 0 69 1 17 7041 0.0 3 6957 80 3 1 18 20 0.0 3 0 19 0 1 19 1 0.0 3 0 0 1 20 3 0.0 3 0 0 0 3 22 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 1 1 63 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 85 16 0.0 3 0 0 0 16 86 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 1 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 136 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 5 0.0 3 0 0 0 5 148 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 5 0.0 3 0 0 0 5 175 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.26 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 7,175 Reads with adapters: 524 (7.3%) Reads written (passing filters): 7,175 (100.0%) Total basepairs processed: 1,981,517 bp Total written (filtered): 1,979,924 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 524 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.3% C: 40.6% G: 14.3% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 511 112.1 0 511 4 7 28.0 0 7 5 4 7.0 0 4 6 2 1.8 0 2