This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.73 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 79,679 (2.3%) Reads that were too short: 25,993 (0.8%) Reads written (passing filters): 53,686 (1.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 11,285,752 bp (1.1%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 79679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 144 846.0 0 144 7 3242 846.0 0 3242 8 215 846.0 0 215 9 6866 846.0 0 6866 10 97 846.0 0 97 11 90 846.0 0 90 12 71 846.0 0 71 13 79 846.0 0 79 14 79 846.0 0 79 15 74 846.0 0 74 16 106 846.0 0 106 17 80 846.0 0 80 18 87 846.0 0 87 19 84 846.0 0 84 20 101 846.0 0 101 21 120 846.0 0 120 22 129 846.0 0 129 23 81 846.0 0 81 24 92 846.0 0 92 25 94 846.0 0 94 26 113 846.0 0 113 27 119 846.0 0 119 28 80 846.0 0 80 29 93 846.0 0 93 30 89 846.0 0 89 31 105 846.0 0 105 32 100 846.0 0 100 33 102 846.0 0 102 34 144 846.0 0 144 35 947 846.0 0 947 36 363 846.0 0 363 37 549 846.0 0 549 38 101 846.0 0 101 39 79 846.0 0 79 40 101 846.0 0 101 41 101 846.0 0 101 42 643 846.0 0 643 43 131 846.0 0 131 44 97 846.0 0 97 45 90 846.0 0 90 46 78 846.0 0 78 47 64 846.0 0 64 48 83 846.0 0 83 49 79 846.0 0 79 50 73 846.0 0 73 51 83 846.0 0 83 52 85 846.0 0 85 53 73 846.0 0 73 54 66 846.0 0 66 55 71 846.0 0 71 56 79 846.0 0 79 57 65 846.0 0 65 58 84 846.0 0 84 59 100 846.0 0 100 60 115 846.0 0 115 61 83 846.0 0 83 62 72 846.0 0 72 63 101 846.0 0 101 64 319 846.0 0 319 65 77 846.0 0 77 66 78 846.0 0 78 67 83 846.0 0 83 68 103 846.0 0 103 69 901 846.0 0 901 70 292 846.0 0 292 71 308 846.0 0 308 72 74 846.0 0 74 73 74 846.0 0 74 74 103 846.0 0 103 75 89 846.0 0 89 76 173 846.0 0 173 77 153 846.0 0 153 78 150 846.0 0 150 79 148 846.0 0 148 80 378 846.0 0 378 81 211 846.0 0 211 82 221 846.0 0 221 83 175 846.0 0 175 84 133 846.0 0 133 85 128 846.0 0 128 86 142 846.0 0 142 87 208 846.0 0 208 88 294 846.0 0 294 89 820 846.0 0 820 90 511 846.0 0 511 91 188 846.0 0 188 92 147 846.0 0 147 93 205 846.0 0 205 94 256 846.0 0 256 95 248 846.0 0 248 96 578 846.0 0 578 97 806 846.0 0 806 98 980 846.0 0 980 99 1551 846.0 0 1551 100 2696 846.0 0 2696 101 453 846.0 0 453 102 185 846.0 0 185 103 148 846.0 0 148 104 118 846.0 0 118 105 304 846.0 0 304 106 170 846.0 0 170 107 249 846.0 0 249 108 177 846.0 0 177 109 135 846.0 0 135 110 425 846.0 0 425 111 601 846.0 0 601 112 565 846.0 0 565 113 238 846.0 0 238 114 203 846.0 0 203 115 149 846.0 0 149 116 110 846.0 0 110 117 138 846.0 0 138 118 153 846.0 0 153 119 200 846.0 0 200 120 219 846.0 0 219 121 426 846.0 0 426 122 490 846.0 0 490 123 287 846.0 0 287 124 175 846.0 0 175 125 177 846.0 0 177 126 194 846.0 0 194 127 267 846.0 0 267 128 226 846.0 0 226 129 224 846.0 0 224 130 186 846.0 0 186 131 271 846.0 0 271 132 229 846.0 0 229 133 225 846.0 0 225 134 234 846.0 0 234 135 185 846.0 0 185 136 160 846.0 0 160 137 176 846.0 0 176 138 158 846.0 0 158 139 271 846.0 0 271 140 293 846.0 0 293 141 559 846.0 0 559 142 193 846.0 0 193 143 319 846.0 0 319 144 168 846.0 0 168 145 155 846.0 0 155 146 324 846.0 0 324 147 249 846.0 0 249 148 217 846.0 0 217 149 185 846.0 0 185 150 152 846.0 0 152 151 117 846.0 0 117 152 171 846.0 0 171 153 166 846.0 0 166 154 173 846.0 0 173 155 225 846.0 0 225 156 169 846.0 0 169 157 173 846.0 0 173 158 161 846.0 0 161 159 396 846.0 0 396 160 230 846.0 0 230 161 266 846.0 0 266 162 177 846.0 0 177 163 136 846.0 0 136 164 148 846.0 0 148 165 159 846.0 0 159 166 158 846.0 0 158 167 176 846.0 0 176 168 274 846.0 0 274 169 166 846.0 0 166 170 159 846.0 0 159 171 109 846.0 0 109 172 147 846.0 0 147 173 202 846.0 0 202 174 183 846.0 0 183 175 186 846.0 0 186 176 146 846.0 0 146 177 192 846.0 0 192 178 129 846.0 0 129 179 154 846.0 0 154 180 131 846.0 0 131 181 168 846.0 0 168 182 187 846.0 0 187 183 161 846.0 0 161 184 193 846.0 0 193 185 228 846.0 0 228 186 167 846.0 0 167 187 168 846.0 0 168 188 141 846.0 0 141 189 158 846.0 0 158 190 189 846.0 0 189 191 192 846.0 0 192 192 145 846.0 0 145 193 188 846.0 0 188 194 261 846.0 0 261 195 344 846.0 0 344 196 190 846.0 0 190 197 405 846.0 0 405 198 126 846.0 0 126 199 179 846.0 0 179 200 320 846.0 0 320 201 349 846.0 0 349 202 425 846.0 0 425 203 272 846.0 0 272 204 234 846.0 0 234 205 226 846.0 0 226 206 540 846.0 0 540 207 732 846.0 0 732 208 530 846.0 0 530 209 444 846.0 0 444 210 266 846.0 0 266 211 240 846.0 0 240 212 213 846.0 0 213 213 203 846.0 0 203 214 233 846.0 0 233 215 175 846.0 0 175 216 179 846.0 0 179 217 151 846.0 0 151 218 168 846.0 0 168 219 167 846.0 0 167 220 249 846.0 0 249 221 294 846.0 0 294 222 341 846.0 0 341 223 250 846.0 0 250 224 169 846.0 0 169 225 189 846.0 0 189 226 148 846.0 0 148 227 147 846.0 0 147 228 154 846.0 0 154 229 234 846.0 0 234 230 243 846.0 0 243 231 282 846.0 0 282 232 261 846.0 0 261 233 271 846.0 0 271 234 236 846.0 0 236 235 266 846.0 0 266 236 410 846.0 0 410 237 478 846.0 0 478 238 250 846.0 0 250 239 394 846.0 0 394 240 334 846.0 0 334 241 845 846.0 0 845 242 827 846.0 0 827 243 298 846.0 0 298 244 177 846.0 0 177 245 151 846.0 0 151 246 105 846.0 0 105 247 109 846.0 0 109 248 228 846.0 0 228 249 338 846.0 0 338 250 538 846.0 0 538 251 191 846.0 0 191 252 168 846.0 0 168 253 144 846.0 0 144 254 177 846.0 0 177 255 190 846.0 0 190 256 197 846.0 0 197 257 156 846.0 0 156 258 183 846.0 0 183 259 332 846.0 0 332 260 770 846.0 0 770 261 849 846.0 0 849 262 570 846.0 0 570 263 169 846.0 0 169 264 164 846.0 0 164 265 138 846.0 0 138 266 95 846.0 0 95 267 103 846.0 0 103 268 152 846.0 0 152 269 172 846.0 0 172 270 240 846.0 0 240 271 182 846.0 0 182 272 204 846.0 0 204 273 172 846.0 0 172 274 199 846.0 0 199 275 326 846.0 0 326 276 154 846.0 0 154 277 136 846.0 0 136 278 142 846.0 0 142 279 133 846.0 0 133 280 156 846.0 0 156 281 226 846.0 0 226 282 301 846.0 0 301 283 321 846.0 0 321 284 275 846.0 0 275 285 124 846.0 0 124 286 100 846.0 0 100 287 123 846.0 0 123 288 156 846.0 0 156 289 357 846.0 0 357 290 186 846.0 0 186 291 150 846.0 0 150 292 177 846.0 0 177 293 141 846.0 0 141 294 186 846.0 0 186 295 385 846.0 0 385 296 206 846.0 0 206 297 185 846.0 0 185 298 163 846.0 0 163 299 165 846.0 0 165 300 233 846.0 0 233 301 255 846.0 0 255 Finished in 2.26 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 53,686 Reads with adapters: 3,148 (5.9%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 3,123 (5.8%) Total basepairs processed: 11,285,752 bp Total written (filtered): 852,377 bp (7.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3148 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 0 1 19 32 0.0 3 0 27 3 2 20 3049 0.0 4 2996 46 5 1 1 21 9 0.0 4 3 4 1 0 1 22 1 0.0 4 1 23 3 0.0 4 1 0 0 1 1 26 1 0.0 4 1 29 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 50 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 76 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 15 0.0 4 0 0 0 0 15 133 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 175 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,123 Reads with adapters: 88 (2.8%) Reads written (passing filters): 3,123 (100.0%) Total basepairs processed: 852,377 bp Total written (filtered): 850,224 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 88 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 3.4% G: 89.8% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 48.8 0 1 10 1 0.0 1 0 1 11 76 0.0 1 1 75 12 5 0.0 1 0 5 171 1 0.0 1 1 257 1 0.0 1 0 1 271 1 0.0 1 1 272 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.