This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 87.44 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 43,654 (1.3%) Reads that were too short: 33,467 (1.0%) Reads written (passing filters): 10,187 (0.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 2,415,239 bp (0.2%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 43654 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 57 846.0 0 57 7 73 846.0 0 73 8 82 846.0 0 82 9 3061 846.0 0 3061 10 29 846.0 0 29 11 25 846.0 0 25 12 27 846.0 0 27 13 22 846.0 0 22 14 22 846.0 0 22 15 24 846.0 0 24 16 26 846.0 0 26 17 34 846.0 0 34 18 35 846.0 0 35 19 30 846.0 0 30 20 20 846.0 0 20 21 31 846.0 0 31 22 30 846.0 0 30 23 31 846.0 0 31 24 20 846.0 0 20 25 24 846.0 0 24 26 30 846.0 0 30 27 22 846.0 0 22 28 19 846.0 0 19 29 20 846.0 0 20 30 31 846.0 0 31 31 13 846.0 0 13 32 31 846.0 0 31 33 40 846.0 0 40 34 44 846.0 0 44 35 21 846.0 0 21 36 38 846.0 0 38 37 34 846.0 0 34 38 44 846.0 0 44 39 45 846.0 0 45 40 34 846.0 0 34 41 41 846.0 0 41 42 37 846.0 0 37 43 37 846.0 0 37 44 26 846.0 0 26 45 21 846.0 0 21 46 30 846.0 0 30 47 32 846.0 0 32 48 26 846.0 0 26 49 34 846.0 0 34 50 46 846.0 0 46 51 31 846.0 0 31 52 1448 846.0 0 1448 53 105 846.0 0 105 54 50 846.0 0 50 55 23 846.0 0 23 56 28 846.0 0 28 57 25 846.0 0 25 58 40 846.0 0 40 59 66 846.0 0 66 60 30 846.0 0 30 61 29 846.0 0 29 62 42 846.0 0 42 63 29 846.0 0 29 64 38 846.0 0 38 65 26 846.0 0 26 66 23 846.0 0 23 67 22 846.0 0 22 68 32 846.0 0 32 69 26 846.0 0 26 70 30 846.0 0 30 71 95 846.0 0 95 72 20 846.0 0 20 73 102 846.0 0 102 74 26 846.0 0 26 75 12 846.0 0 12 76 18 846.0 0 18 77 20 846.0 0 20 78 27 846.0 0 27 79 15 846.0 0 15 80 21 846.0 0 21 81 26 846.0 0 26 82 17 846.0 0 17 83 20 846.0 0 20 84 22 846.0 0 22 85 14 846.0 0 14 86 18 846.0 0 18 87 16 846.0 0 16 88 11 846.0 0 11 89 13 846.0 0 13 90 21 846.0 0 21 91 23 846.0 0 23 92 25 846.0 0 25 93 24 846.0 0 24 94 14 846.0 0 14 95 30 846.0 0 30 96 22 846.0 0 22 97 18 846.0 0 18 98 27 846.0 0 27 99 29 846.0 0 29 100 21 846.0 0 21 101 26 846.0 0 26 102 35 846.0 0 35 103 19 846.0 0 19 104 14 846.0 0 14 105 13 846.0 0 13 106 11 846.0 0 11 107 13 846.0 0 13 108 15 846.0 0 15 109 26 846.0 0 26 110 23 846.0 0 23 111 30 846.0 0 30 112 23 846.0 0 23 113 18 846.0 0 18 114 19 846.0 0 19 115 24 846.0 0 24 116 26 846.0 0 26 117 44 846.0 0 44 118 28 846.0 0 28 119 20 846.0 0 20 120 240 846.0 0 240 121 23 846.0 0 23 122 20 846.0 0 20 123 37 846.0 0 37 124 21 846.0 0 21 125 13 846.0 0 13 126 18 846.0 0 18 127 15 846.0 0 15 128 23 846.0 0 23 129 32 846.0 0 32 130 175 846.0 0 175 131 58 846.0 0 58 132 212 846.0 0 212 133 58 846.0 0 58 134 15 846.0 0 15 135 27 846.0 0 27 136 21 846.0 0 21 137 91 846.0 0 91 138 51 846.0 0 51 139 8 846.0 0 8 140 15 846.0 0 15 141 11 846.0 0 11 142 24 846.0 0 24 143 13 846.0 0 13 144 12 846.0 0 12 145 13 846.0 0 13 146 15 846.0 0 15 147 13 846.0 0 13 148 16 846.0 0 16 149 16 846.0 0 16 150 22 846.0 0 22 151 20 846.0 0 20 152 17 846.0 0 17 153 43 846.0 0 43 154 9 846.0 0 9 155 14 846.0 0 14 156 26 846.0 0 26 157 14 846.0 0 14 158 14 846.0 0 14 159 17 846.0 0 17 160 15 846.0 0 15 161 16 846.0 0 16 162 14 846.0 0 14 163 18 846.0 0 18 164 21 846.0 0 21 165 32 846.0 0 32 166 9 846.0 0 9 167 18 846.0 0 18 168 9 846.0 0 9 169 10 846.0 0 10 170 13 846.0 0 13 171 5 846.0 0 5 172 14 846.0 0 14 173 16 846.0 0 16 174 13 846.0 0 13 175 14 846.0 0 14 176 9 846.0 0 9 177 22 846.0 0 22 178 23 846.0 0 23 179 20 846.0 0 20 180 20 846.0 0 20 181 13 846.0 0 13 182 12 846.0 0 12 183 25 846.0 0 25 184 19 846.0 0 19 185 17 846.0 0 17 186 49 846.0 0 49 187 35 846.0 0 35 188 53 846.0 0 53 189 23 846.0 0 23 190 11 846.0 0 11 191 19 846.0 0 19 192 16 846.0 0 16 193 29 846.0 0 29 194 48 846.0 0 48 195 62 846.0 0 62 196 19 846.0 0 19 197 12 846.0 0 12 198 13 846.0 0 13 199 23 846.0 0 23 200 14 846.0 0 14 201 6 846.0 0 6 202 17 846.0 0 17 203 126 846.0 0 126 204 39 846.0 0 39 205 67 846.0 0 67 206 23 846.0 0 23 207 19 846.0 0 19 208 14 846.0 0 14 209 8 846.0 0 8 210 19 846.0 0 19 211 11 846.0 0 11 212 129 846.0 0 129 213 108 846.0 0 108 214 130 846.0 0 130 215 21 846.0 0 21 216 37 846.0 0 37 217 18 846.0 0 18 218 35 846.0 0 35 219 199 846.0 0 199 220 22 846.0 0 22 221 13 846.0 0 13 222 17 846.0 0 17 223 8 846.0 0 8 224 18 846.0 0 18 225 21 846.0 0 21 226 21 846.0 0 21 227 11 846.0 0 11 228 14 846.0 0 14 229 13 846.0 0 13 230 20 846.0 0 20 231 16 846.0 0 16 232 64 846.0 0 64 233 100 846.0 0 100 234 21 846.0 0 21 235 49 846.0 0 49 236 119 846.0 0 119 237 40 846.0 0 40 238 44 846.0 0 44 239 21 846.0 0 21 240 13 846.0 0 13 241 22 846.0 0 22 242 17 846.0 0 17 243 72 846.0 0 72 244 34 846.0 0 34 245 68 846.0 0 68 246 40 846.0 0 40 247 18 846.0 0 18 248 26 846.0 0 26 249 11 846.0 0 11 250 12 846.0 0 12 251 16 846.0 0 16 252 14 846.0 0 14 253 11 846.0 0 11 254 29 846.0 0 29 255 46 846.0 0 46 256 198 846.0 0 198 257 75 846.0 0 75 258 50 846.0 0 50 259 39 846.0 0 39 260 140 846.0 0 140 261 258 846.0 0 258 262 685 846.0 0 685 263 65 846.0 0 65 264 17 846.0 0 17 265 84 846.0 0 84 266 18 846.0 0 18 267 21 846.0 0 21 268 49 846.0 0 49 269 26 846.0 0 26 270 21 846.0 0 21 271 39 846.0 0 39 272 107 846.0 0 107 273 273 846.0 0 273 274 65 846.0 0 65 275 136 846.0 0 136 276 175 846.0 0 175 277 95 846.0 0 95 278 98 846.0 0 98 279 237 846.0 0 237 280 128 846.0 0 128 281 206 846.0 0 206 282 186 846.0 0 186 283 252 846.0 0 252 284 189 846.0 0 189 285 286 846.0 0 286 286 3212 846.0 0 3212 287 7226 846.0 0 7226 288 16312 846.0 0 16312 289 80 846.0 0 80 290 10 846.0 0 10 291 19 846.0 0 19 292 20 846.0 0 20 293 15 846.0 0 15 294 21 846.0 0 21 295 13 846.0 0 13 296 36 846.0 0 36 297 21 846.0 0 21 298 87 846.0 0 87 299 24 846.0 0 24 300 20 846.0 0 20 301 12 846.0 0 12 Finished in 0.44 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 10,187 Reads with adapters: 3,113 (30.6%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 3,111 (30.5%) Total basepairs processed: 2,415,239 bp Total written (filtered): 854,586 bp (35.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3113 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 30 0.0 3 0 29 1 17 3052 0.0 3 2984 66 0 2 18 16 0.0 3 0 9 4 3 19 2 0.0 3 0 0 1 1 20 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 3,111 Reads with adapters: 165 (5.3%) Reads written (passing filters): 3,111 (100.0%) Total basepairs processed: 854,586 bp Total written (filtered): 854,091 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 165 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.9% C: 25.5% G: 40.0% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 165 48.6 0 165