This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.48 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 45,220 (1.3%) Reads that were too short: 12,929 (0.4%) Reads written (passing filters): 32,291 (0.9%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 7,852,611 bp (0.8%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 45220 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 199 846.0 0 199 7 212 846.0 0 212 8 19132 846.0 0 19132 9 56 846.0 0 56 10 17 846.0 0 17 11 17 846.0 0 17 12 8 846.0 0 8 13 17 846.0 0 17 14 10 846.0 0 10 15 15 846.0 0 15 16 23 846.0 0 23 17 24 846.0 0 24 18 17 846.0 0 17 19 22 846.0 0 22 20 13 846.0 0 13 21 21 846.0 0 21 22 15 846.0 0 15 23 14 846.0 0 14 24 17 846.0 0 17 25 21 846.0 0 21 26 20 846.0 0 20 27 14 846.0 0 14 28 21 846.0 0 21 29 14 846.0 0 14 30 12 846.0 0 12 31 16 846.0 0 16 32 25 846.0 0 25 33 13 846.0 0 13 34 27 846.0 0 27 35 14 846.0 0 14 36 22 846.0 0 22 37 23 846.0 0 23 38 39 846.0 0 39 39 20 846.0 0 20 40 41 846.0 0 41 41 23 846.0 0 23 42 28 846.0 0 28 43 21 846.0 0 21 44 20 846.0 0 20 45 24 846.0 0 24 46 20 846.0 0 20 47 20 846.0 0 20 48 38 846.0 0 38 49 48 846.0 0 48 50 23 846.0 0 23 51 24 846.0 0 24 52 22 846.0 0 22 53 23 846.0 0 23 54 16 846.0 0 16 55 23 846.0 0 23 56 23 846.0 0 23 57 24 846.0 0 24 58 19 846.0 0 19 59 21 846.0 0 21 60 26 846.0 0 26 61 35 846.0 0 35 62 26 846.0 0 26 63 57 846.0 0 57 64 37 846.0 0 37 65 67 846.0 0 67 66 34 846.0 0 34 67 39 846.0 0 39 68 20 846.0 0 20 69 23 846.0 0 23 70 28 846.0 0 28 71 20 846.0 0 20 72 28 846.0 0 28 73 28 846.0 0 28 74 83 846.0 0 83 75 23 846.0 0 23 76 29 846.0 0 29 77 25 846.0 0 25 78 38 846.0 0 38 79 43 846.0 0 43 80 21 846.0 0 21 81 18 846.0 0 18 82 24 846.0 0 24 83 21 846.0 0 21 84 37 846.0 0 37 85 38 846.0 0 38 86 24 846.0 0 24 87 25 846.0 0 25 88 36 846.0 0 36 89 23 846.0 0 23 90 22 846.0 0 22 91 37 846.0 0 37 92 19 846.0 0 19 93 38 846.0 0 38 94 73 846.0 0 73 95 233 846.0 0 233 96 68 846.0 0 68 97 292 846.0 0 292 98 452 846.0 0 452 99 179 846.0 0 179 100 192 846.0 0 192 101 39 846.0 0 39 102 44 846.0 0 44 103 35 846.0 0 35 104 36 846.0 0 36 105 28 846.0 0 28 106 23 846.0 0 23 107 20 846.0 0 20 108 35 846.0 0 35 109 85 846.0 0 85 110 34 846.0 0 34 111 67 846.0 0 67 112 447 846.0 0 447 113 260 846.0 0 260 114 30 846.0 0 30 115 36 846.0 0 36 116 36 846.0 0 36 117 37 846.0 0 37 118 27 846.0 0 27 119 30 846.0 0 30 120 39 846.0 0 39 121 35 846.0 0 35 122 48 846.0 0 48 123 34 846.0 0 34 124 29 846.0 0 29 125 54 846.0 0 54 126 37 846.0 0 37 127 49 846.0 0 49 128 67 846.0 0 67 129 142 846.0 0 142 130 60 846.0 0 60 131 93 846.0 0 93 132 498 846.0 0 498 133 162 846.0 0 162 134 189 846.0 0 189 135 81 846.0 0 81 136 104 846.0 0 104 137 43 846.0 0 43 138 54 846.0 0 54 139 42 846.0 0 42 140 49 846.0 0 49 141 44 846.0 0 44 142 45 846.0 0 45 143 26 846.0 0 26 144 40 846.0 0 40 145 47 846.0 0 47 146 81 846.0 0 81 147 65 846.0 0 65 148 47 846.0 0 47 149 53 846.0 0 53 150 47 846.0 0 47 151 33 846.0 0 33 152 32 846.0 0 32 153 32 846.0 0 32 154 111 846.0 0 111 155 110 846.0 0 110 156 111 846.0 0 111 157 60 846.0 0 60 158 35 846.0 0 35 159 52 846.0 0 52 160 71 846.0 0 71 161 57 846.0 0 57 162 42 846.0 0 42 163 26 846.0 0 26 164 46 846.0 0 46 165 38 846.0 0 38 166 112 846.0 0 112 167 70 846.0 0 70 168 68 846.0 0 68 169 50 846.0 0 50 170 31 846.0 0 31 171 51 846.0 0 51 172 30 846.0 0 30 173 46 846.0 0 46 174 163 846.0 0 163 175 108 846.0 0 108 176 68 846.0 0 68 177 48 846.0 0 48 178 64 846.0 0 64 179 125 846.0 0 125 180 137 846.0 0 137 181 192 846.0 0 192 182 80 846.0 0 80 183 76 846.0 0 76 184 77 846.0 0 77 185 75 846.0 0 75 186 85 846.0 0 85 187 68 846.0 0 68 188 44 846.0 0 44 189 41 846.0 0 41 190 60 846.0 0 60 191 56 846.0 0 56 192 78 846.0 0 78 193 160 846.0 0 160 194 531 846.0 0 531 195 461 846.0 0 461 196 403 846.0 0 403 197 94 846.0 0 94 198 104 846.0 0 104 199 206 846.0 0 206 200 176 846.0 0 176 201 212 846.0 0 212 202 150 846.0 0 150 203 51 846.0 0 51 204 99 846.0 0 99 205 101 846.0 0 101 206 98 846.0 0 98 207 99 846.0 0 99 208 103 846.0 0 103 209 69 846.0 0 69 210 61 846.0 0 61 211 69 846.0 0 69 212 72 846.0 0 72 213 51 846.0 0 51 214 69 846.0 0 69 215 136 846.0 0 136 216 108 846.0 0 108 217 130 846.0 0 130 218 78 846.0 0 78 219 108 846.0 0 108 220 527 846.0 0 527 221 364 846.0 0 364 222 309 846.0 0 309 223 173 846.0 0 173 224 66 846.0 0 66 225 53 846.0 0 53 226 64 846.0 0 64 227 72 846.0 0 72 228 51 846.0 0 51 229 93 846.0 0 93 230 41 846.0 0 41 231 77 846.0 0 77 232 50 846.0 0 50 233 67 846.0 0 67 234 67 846.0 0 67 235 84 846.0 0 84 236 77 846.0 0 77 237 222 846.0 0 222 238 382 846.0 0 382 239 156 846.0 0 156 240 104 846.0 0 104 241 124 846.0 0 124 242 103 846.0 0 103 243 258 846.0 0 258 244 191 846.0 0 191 245 88 846.0 0 88 246 81 846.0 0 81 247 98 846.0 0 98 248 78 846.0 0 78 249 46 846.0 0 46 250 44 846.0 0 44 251 53 846.0 0 53 252 77 846.0 0 77 253 124 846.0 0 124 254 219 846.0 0 219 255 190 846.0 0 190 256 354 846.0 0 354 257 126 846.0 0 126 258 181 846.0 0 181 259 89 846.0 0 89 260 78 846.0 0 78 261 58 846.0 0 58 262 701 846.0 0 701 263 410 846.0 0 410 264 1572 846.0 0 1572 265 88 846.0 0 88 266 85 846.0 0 85 267 89 846.0 0 89 268 256 846.0 0 256 269 173 846.0 0 173 270 282 846.0 0 282 271 53 846.0 0 53 272 45 846.0 0 45 273 54 846.0 0 54 274 61 846.0 0 61 275 56 846.0 0 56 276 47 846.0 0 47 277 44 846.0 0 44 278 38 846.0 0 38 279 65 846.0 0 65 280 108 846.0 0 108 281 91 846.0 0 91 282 90 846.0 0 90 283 59 846.0 0 59 284 61 846.0 0 61 285 84 846.0 0 84 286 92 846.0 0 92 287 63 846.0 0 63 288 46 846.0 0 46 289 50 846.0 0 50 290 39 846.0 0 39 291 69 846.0 0 69 292 55 846.0 0 55 293 71 846.0 0 71 294 83 846.0 0 83 295 99 846.0 0 99 296 24 846.0 0 24 297 20 846.0 0 20 298 19 846.0 0 19 299 19 846.0 0 19 300 43 846.0 0 43 301 114 846.0 0 114 Finished in 1.36 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 32,291 Reads with adapters: 19,493 (60.4%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 19,485 (60.3%) Total basepairs processed: 7,852,611 bp Total written (filtered): 5,376,776 bp (68.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19493 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 159 0.0 3 0 156 3 17 19179 0.0 3 18929 246 4 18 77 0.0 3 25 47 4 1 19 13 0.0 3 0 0 7 6 20 38 0.0 3 0 0 0 38 23 1 0.0 3 1 37 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 77 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 3 0.0 3 0 0 0 3 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.67 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 19,485 Reads with adapters: 386 (2.0%) Reads written (passing filters): 19,485 (100.0%) Total basepairs processed: 5,376,776 bp Total written (filtered): 5,375,602 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 386 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.3% C: 22.5% G: 32.9% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 380 304.5 0 380 4 5 76.1 0 5 14 1 0.0 1 0 1