This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.65 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 171,660 (5.0%) Reads that were too short: 12,133 (0.4%) Reads written (passing filters): 159,527 (4.6%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 26,609,866 bp (2.6%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 171660 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 69 846.0 0 69 7 1799 846.0 0 1799 8 80 846.0 0 80 9 67 846.0 0 67 10 72 846.0 0 72 11 627 846.0 0 627 12 75 846.0 0 75 13 61 846.0 0 61 14 56 846.0 0 56 15 68 846.0 0 68 16 58 846.0 0 58 17 66 846.0 0 66 18 83 846.0 0 83 19 68 846.0 0 68 20 76 846.0 0 76 21 59 846.0 0 59 22 66 846.0 0 66 23 84 846.0 0 84 24 64 846.0 0 64 25 47 846.0 0 47 26 53 846.0 0 53 27 64 846.0 0 64 28 47 846.0 0 47 29 126 846.0 0 126 30 51 846.0 0 51 31 57 846.0 0 57 32 50 846.0 0 50 33 47 846.0 0 47 34 39 846.0 0 39 35 85 846.0 0 85 36 67 846.0 0 67 37 82 846.0 0 82 38 96 846.0 0 96 39 175 846.0 0 175 40 71 846.0 0 71 41 78 846.0 0 78 42 61 846.0 0 61 43 82 846.0 0 82 44 112 846.0 0 112 45 88 846.0 0 88 46 85 846.0 0 85 47 85 846.0 0 85 48 71 846.0 0 71 49 99 846.0 0 99 50 95 846.0 0 95 51 198 846.0 0 198 52 310 846.0 0 310 53 421 846.0 0 421 54 523 846.0 0 523 55 769 846.0 0 769 56 29876 846.0 0 29876 57 10509 846.0 0 10509 58 16120 846.0 0 16120 59 109 846.0 0 109 60 52 846.0 0 52 61 52 846.0 0 52 62 64 846.0 0 64 63 130 846.0 0 130 64 51 846.0 0 51 65 37 846.0 0 37 66 49 846.0 0 49 67 53 846.0 0 53 68 50 846.0 0 50 69 44 846.0 0 44 70 51 846.0 0 51 71 41 846.0 0 41 72 58 846.0 0 58 73 64 846.0 0 64 74 74 846.0 0 74 75 50 846.0 0 50 76 43 846.0 0 43 77 39 846.0 0 39 78 41 846.0 0 41 79 45 846.0 0 45 80 126 846.0 0 126 81 70 846.0 0 70 82 65 846.0 0 65 83 114 846.0 0 114 84 199 846.0 0 199 85 67 846.0 0 67 86 58 846.0 0 58 87 282 846.0 0 282 88 198 846.0 0 198 89 534 846.0 0 534 90 66 846.0 0 66 91 32 846.0 0 32 92 81 846.0 0 81 93 93 846.0 0 93 94 68 846.0 0 68 95 102 846.0 0 102 96 44 846.0 0 44 97 42 846.0 0 42 98 41 846.0 0 41 99 57 846.0 0 57 100 55 846.0 0 55 101 81 846.0 0 81 102 56 846.0 0 56 103 225 846.0 0 225 104 74 846.0 0 74 105 85 846.0 0 85 106 29 846.0 0 29 107 40 846.0 0 40 108 45 846.0 0 45 109 53 846.0 0 53 110 60 846.0 0 60 111 79 846.0 0 79 112 44 846.0 0 44 113 53 846.0 0 53 114 64 846.0 0 64 115 51 846.0 0 51 116 65 846.0 0 65 117 56 846.0 0 56 118 59 846.0 0 59 119 72 846.0 0 72 120 72 846.0 0 72 121 65 846.0 0 65 122 60 846.0 0 60 123 88 846.0 0 88 124 284 846.0 0 284 125 311 846.0 0 311 126 754 846.0 0 754 127 104 846.0 0 104 128 111 846.0 0 111 129 40 846.0 0 40 130 63 846.0 0 63 131 58 846.0 0 58 132 69 846.0 0 69 133 47 846.0 0 47 134 64 846.0 0 64 135 85 846.0 0 85 136 50 846.0 0 50 137 65 846.0 0 65 138 75 846.0 0 75 139 54 846.0 0 54 140 75 846.0 0 75 141 86 846.0 0 86 142 74 846.0 0 74 143 63 846.0 0 63 144 84 846.0 0 84 145 93 846.0 0 93 146 287 846.0 0 287 147 651 846.0 0 651 148 291 846.0 0 291 149 328 846.0 0 328 150 887 846.0 0 887 151 204 846.0 0 204 152 221 846.0 0 221 153 95 846.0 0 95 154 58 846.0 0 58 155 68 846.0 0 68 156 144 846.0 0 144 157 81 846.0 0 81 158 96 846.0 0 96 159 74 846.0 0 74 160 157 846.0 0 157 161 121 846.0 0 121 162 124 846.0 0 124 163 102 846.0 0 102 164 95 846.0 0 95 165 116 846.0 0 116 166 135 846.0 0 135 167 929 846.0 0 929 168 488 846.0 0 488 169 998 846.0 0 998 170 321 846.0 0 321 171 180 846.0 0 180 172 196 846.0 0 196 173 305 846.0 0 305 174 195 846.0 0 195 175 369 846.0 0 369 176 407 846.0 0 407 177 346 846.0 0 346 178 137 846.0 0 137 179 123 846.0 0 123 180 167 846.0 0 167 181 147 846.0 0 147 182 111 846.0 0 111 183 159 846.0 0 159 184 106 846.0 0 106 185 118 846.0 0 118 186 114 846.0 0 114 187 96 846.0 0 96 188 119 846.0 0 119 189 124 846.0 0 124 190 107 846.0 0 107 191 176 846.0 0 176 192 215 846.0 0 215 193 147 846.0 0 147 194 174 846.0 0 174 195 761 846.0 0 761 196 543 846.0 0 543 197 739 846.0 0 739 198 716 846.0 0 716 199 755 846.0 0 755 200 6460 846.0 0 6460 201 65931 846.0 0 65931 202 3216 846.0 0 3216 203 117 846.0 0 117 204 52 846.0 0 52 205 80 846.0 0 80 206 66 846.0 0 66 207 75 846.0 0 75 208 59 846.0 0 59 209 55 846.0 0 55 210 53 846.0 0 53 211 47 846.0 0 47 212 55 846.0 0 55 213 78 846.0 0 78 214 72 846.0 0 72 215 71 846.0 0 71 216 65 846.0 0 65 217 85 846.0 0 85 218 74 846.0 0 74 219 131 846.0 0 131 220 157 846.0 0 157 221 109 846.0 0 109 222 58 846.0 0 58 223 47 846.0 0 47 224 63 846.0 0 63 225 76 846.0 0 76 226 68 846.0 0 68 227 72 846.0 0 72 228 101 846.0 0 101 229 426 846.0 0 426 230 153 846.0 0 153 231 181 846.0 0 181 232 219 846.0 0 219 233 326 846.0 0 326 234 147 846.0 0 147 235 51 846.0 0 51 236 58 846.0 0 58 237 52 846.0 0 52 238 55 846.0 0 55 239 74 846.0 0 74 240 78 846.0 0 78 241 57 846.0 0 57 242 62 846.0 0 62 243 61 846.0 0 61 244 60 846.0 0 60 245 49 846.0 0 49 246 69 846.0 0 69 247 49 846.0 0 49 248 40 846.0 0 40 249 47 846.0 0 47 250 72 846.0 0 72 251 64 846.0 0 64 252 68 846.0 0 68 253 93 846.0 0 93 254 52 846.0 0 52 255 86 846.0 0 86 256 127 846.0 0 127 257 106 846.0 0 106 258 71 846.0 0 71 259 48 846.0 0 48 260 72 846.0 0 72 261 61 846.0 0 61 262 69 846.0 0 69 263 96 846.0 0 96 264 92 846.0 0 92 265 88 846.0 0 88 266 87 846.0 0 87 267 90 846.0 0 90 268 98 846.0 0 98 269 76 846.0 0 76 270 38 846.0 0 38 271 57 846.0 0 57 272 62 846.0 0 62 273 335 846.0 0 335 274 160 846.0 0 160 275 88 846.0 0 88 276 94 846.0 0 94 277 97 846.0 0 97 278 187 846.0 0 187 279 117 846.0 0 117 280 114 846.0 0 114 281 96 846.0 0 96 282 100 846.0 0 100 283 92 846.0 0 92 284 89 846.0 0 89 285 56 846.0 0 56 286 57 846.0 0 57 287 55 846.0 0 55 288 54 846.0 0 54 289 68 846.0 0 68 290 56 846.0 0 56 291 104 846.0 0 104 292 153 846.0 0 153 293 139 846.0 0 139 294 67 846.0 0 67 295 34 846.0 0 34 296 39 846.0 0 39 297 37 846.0 0 37 298 36 846.0 0 36 299 73 846.0 0 73 300 163 846.0 0 163 301 84 846.0 0 84 Finished in 5.08 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 159,527 Reads with adapters: 1,809 (1.1%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 1,781 (1.1%) Total basepairs processed: 26,609,866 bp Total written (filtered): 490,736 bp (1.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1809 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 18 0.0 3 0 16 2 17 1740 0.0 3 1725 15 18 6 0.0 3 1 2 2 1 20 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 88 4 0.0 3 0 0 0 4 89 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 232 5 0.0 3 0 0 0 5 233 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 1,781 Reads with adapters: 120 (6.7%) Reads written (passing filters): 1,781 (100.0%) Total basepairs processed: 490,736 bp Total written (filtered): 490,368 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 120 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.3% C: 45.8% G: 15.0% T: 10.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 115 27.8 0 115 4 4 7.0 0 4 7 1 0.1 0 1