This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.85 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 583,890 (21.6%) Reads that were too short: 348,205 (12.9%) Reads written (passing filters): 235,685 (8.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 61,186,846 bp (7.5%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 583890 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6833 661.3 0 6833 7 177991 661.3 0 177991 8 723 661.3 0 723 9 109 661.3 0 109 10 48 661.3 0 48 11 31 661.3 0 31 12 21 661.3 0 21 13 38 661.3 0 38 14 32 661.3 0 32 15 17 661.3 0 17 16 29 661.3 0 29 17 63 661.3 0 63 18 34 661.3 0 34 19 41 661.3 0 41 20 27 661.3 0 27 21 33 661.3 0 33 22 17 661.3 0 17 23 13 661.3 0 13 24 26 661.3 0 26 25 30 661.3 0 30 26 21 661.3 0 21 27 25 661.3 0 25 28 21 661.3 0 21 29 28 661.3 0 28 30 39 661.3 0 39 31 88 661.3 0 88 32 777 661.3 0 777 33 44 661.3 0 44 34 33 661.3 0 33 35 30 661.3 0 30 36 29 661.3 0 29 37 29 661.3 0 29 38 25 661.3 0 25 39 23 661.3 0 23 40 23 661.3 0 23 41 33 661.3 0 33 42 25 661.3 0 25 43 25 661.3 0 25 44 23 661.3 0 23 45 41 661.3 0 41 46 23 661.3 0 23 47 30 661.3 0 30 48 24 661.3 0 24 49 14 661.3 0 14 50 30 661.3 0 30 51 24 661.3 0 24 52 31 661.3 0 31 53 22 661.3 0 22 54 21 661.3 0 21 55 61 661.3 0 61 56 30 661.3 0 30 57 35 661.3 0 35 58 34 661.3 0 34 59 30 661.3 0 30 60 32 661.3 0 32 61 29 661.3 0 29 62 25 661.3 0 25 63 18 661.3 0 18 64 25 661.3 0 25 65 37 661.3 0 37 66 39 661.3 0 39 67 29 661.3 0 29 68 17 661.3 0 17 69 17 661.3 0 17 70 24 661.3 0 24 71 30 661.3 0 30 72 31 661.3 0 31 73 187 661.3 0 187 74 28 661.3 0 28 75 200 661.3 0 200 76 121 661.3 0 121 77 162 661.3 0 162 78 25 661.3 0 25 79 15 661.3 0 15 80 20 661.3 0 20 81 28 661.3 0 28 82 18 661.3 0 18 83 11 661.3 0 11 84 20 661.3 0 20 85 17 661.3 0 17 86 22 661.3 0 22 87 16 661.3 0 16 88 16 661.3 0 16 89 20 661.3 0 20 90 15 661.3 0 15 91 27 661.3 0 27 92 35 661.3 0 35 93 30 661.3 0 30 94 27 661.3 0 27 95 21 661.3 0 21 96 25 661.3 0 25 97 25 661.3 0 25 98 28 661.3 0 28 99 36 661.3 0 36 100 84 661.3 0 84 101 31 661.3 0 31 102 51 661.3 0 51 103 54 661.3 0 54 104 36 661.3 0 36 105 27 661.3 0 27 106 35 661.3 0 35 107 52 661.3 0 52 108 45 661.3 0 45 109 35 661.3 0 35 110 39 661.3 0 39 111 43 661.3 0 43 112 64 661.3 0 64 113 502 661.3 0 502 114 582 661.3 0 582 115 3148 661.3 0 3148 116 23 661.3 0 23 117 12 661.3 0 12 118 18 661.3 0 18 119 15 661.3 0 15 120 15 661.3 0 15 121 84 661.3 0 84 122 17 661.3 0 17 123 17 661.3 0 17 124 16 661.3 0 16 125 33 661.3 0 33 126 26 661.3 0 26 127 141 661.3 0 141 128 31 661.3 0 31 129 28 661.3 0 28 130 27 661.3 0 27 131 32 661.3 0 32 132 29 661.3 0 29 133 54 661.3 0 54 134 88 661.3 0 88 135 45 661.3 0 45 136 49 661.3 0 49 137 75 661.3 0 75 138 89 661.3 0 89 139 39 661.3 0 39 140 95 661.3 0 95 141 50 661.3 0 50 142 225 661.3 0 225 143 178 661.3 0 178 144 650 661.3 0 650 145 78 661.3 0 78 146 105 661.3 0 105 147 710 661.3 0 710 148 3497 661.3 0 3497 149 2030 661.3 0 2030 150 215 661.3 0 215 151 24 661.3 0 24 152 14 661.3 0 14 153 22 661.3 0 22 154 15 661.3 0 15 155 27 661.3 0 27 156 17 661.3 0 17 157 18 661.3 0 18 158 15 661.3 0 15 159 18 661.3 0 18 160 28 661.3 0 28 161 26 661.3 0 26 162 32 661.3 0 32 163 21 661.3 0 21 164 32 661.3 0 32 165 39 661.3 0 39 166 19 661.3 0 19 167 26 661.3 0 26 168 27 661.3 0 27 169 31 661.3 0 31 170 18 661.3 0 18 171 24 661.3 0 24 172 29 661.3 0 29 173 35 661.3 0 35 174 34 661.3 0 34 175 25 661.3 0 25 176 41 661.3 0 41 177 23 661.3 0 23 178 56 661.3 0 56 179 33 661.3 0 33 180 50 661.3 0 50 181 75 661.3 0 75 182 59 661.3 0 59 183 59 661.3 0 59 184 78 661.3 0 78 185 133 661.3 0 133 186 142 661.3 0 142 187 292 661.3 0 292 188 248 661.3 0 248 189 745 661.3 0 745 190 526 661.3 0 526 191 860 661.3 0 860 192 1026 661.3 0 1026 193 590 661.3 0 590 194 617 661.3 0 617 195 819 661.3 0 819 196 672 661.3 0 672 197 666 661.3 0 666 198 802 661.3 0 802 199 1047 661.3 0 1047 200 5600 661.3 0 5600 201 16651 661.3 0 16651 202 7241 661.3 0 7241 203 1745 661.3 0 1745 204 1911 661.3 0 1911 205 11300 661.3 0 11300 206 25902 661.3 0 25902 207 48976 661.3 0 48976 208 14602 661.3 0 14602 209 6822 661.3 0 6822 210 1481 661.3 0 1481 211 2526 661.3 0 2526 212 3151 661.3 0 3151 213 2887 661.3 0 2887 214 705 661.3 0 705 215 1021 661.3 0 1021 216 975 661.3 0 975 217 813 661.3 0 813 218 1486 661.3 0 1486 219 2939 661.3 0 2939 220 3146 661.3 0 3146 221 3324 661.3 0 3324 222 3323 661.3 0 3323 223 2249 661.3 0 2249 224 2400 661.3 0 2400 225 7677 661.3 0 7677 226 38132 661.3 0 38132 227 76969 661.3 0 76969 228 36079 661.3 0 36079 229 288 661.3 0 288 230 87 661.3 0 87 231 162 661.3 0 162 232 27 661.3 0 27 233 28 661.3 0 28 234 14 661.3 0 14 235 16 661.3 0 16 236 32 661.3 0 32 237 15 661.3 0 15 238 22 661.3 0 22 239 27 661.3 0 27 240 36 661.3 0 36 241 31 661.3 0 31 242 31 661.3 0 31 243 29 661.3 0 29 244 146 661.3 0 146 245 260 661.3 0 260 246 268 661.3 0 268 247 86 661.3 0 86 248 173 661.3 0 173 249 372 661.3 0 372 250 89 661.3 0 89 251 244 661.3 0 244 252 278 661.3 0 278 253 328 661.3 0 328 254 154 661.3 0 154 255 342 661.3 0 342 256 217 661.3 0 217 257 433 661.3 0 433 258 232 661.3 0 232 259 306 661.3 0 306 260 307 661.3 0 307 261 377 661.3 0 377 262 4116 661.3 0 4116 263 8013 661.3 0 8013 264 19509 661.3 0 19509 265 211 661.3 0 211 266 67 661.3 0 67 267 16 661.3 0 16 268 24 661.3 0 24 269 21 661.3 0 21 270 31 661.3 0 31 271 27 661.3 0 27 272 65 661.3 0 65 273 15 661.3 0 15 274 41 661.3 0 41 275 41 661.3 0 41 276 18 661.3 0 18 277 10 661.3 0 10 278 16 661.3 0 16 279 20 661.3 0 20 280 17 661.3 0 17 281 12 661.3 0 12 282 11 661.3 0 11 283 33 661.3 0 33 284 23 661.3 0 23 285 24 661.3 0 24 286 18 661.3 0 18 287 19 661.3 0 19 288 11 661.3 0 11 289 21 661.3 0 21 290 26 661.3 0 26 291 24 661.3 0 24 292 100 661.3 0 100 293 97 661.3 0 97 294 85 661.3 0 85 295 33 661.3 0 33 296 75 661.3 0 75 297 10 661.3 0 10 298 30 661.3 0 30 299 11 661.3 0 11 300 10 661.3 0 10 301 15 661.3 0 15 Finished in 9.75 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 235,685 Reads with adapters: 185,067 (78.5%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 185,051 (78.5%) Total basepairs processed: 61,186,846 bp Total written (filtered): 50,711,153 bp (82.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 185067 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 5 1 18 83 0.0 3 0 0 71 11 1 19 2634 0.0 3 0 2554 54 19 7 20 181669 0.0 4 178252 2976 312 99 30 21 470 0.0 4 148 311 8 2 1 22 44 0.0 4 4 3 32 0 5 23 133 0.0 4 0 0 1 116 16 24 5 0.0 4 0 0 0 1 4 25 3 0.0 4 3 27 1 0.0 4 1 31 1 0.0 4 1 40 1 0.0 4 1 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 4 0.0 4 0 0 0 0 4 74 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 3 0.0 4 0 0 0 0 3 107 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.26 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 185,051 Reads with adapters: 3,242 (1.8%) Reads written (passing filters): 185,051 (100.0%) Total basepairs processed: 50,711,153 bp Total written (filtered): 50,565,652 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3242 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.6% C: 7.4% G: 74.2% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 99 2891.4 0 99 4 4 722.9 0 4 5 1 180.7 0 1 10 16 0.2 1 0 16 11 2267 0.0 1 16 2251 12 313 0.0 1 5 308 13 17 0.0 1 2 15 15 5 0.0 1 0 5 25 10 0.0 1 6 4 26 1 0.0 1 0 1 64 1 0.0 1 0 1 91 1 0.0 1 1 140 2 0.0 1 2 149 1 0.0 1 1 152 46 0.0 1 35 11 208 1 0.0 1 1 210 216 0.0 1 193 23 211 11 0.0 1 9 2 260 3 0.0 1 3 261 115 0.0 1 109 6 262 48 0.0 1 44 4 263 6 0.0 1 6 264 49 0.0 1 49 265 5 0.0 1 5 269 1 0.0 1 1 271 2 0.0 1 2 272 1 0.0 1 1