This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.22 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 60,253 (2.2%) Reads that were too short: 7,549 (0.3%) Reads written (passing filters): 52,704 (1.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 11,983,153 bp (1.5%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 60253 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 567 661.3 0 567 7 387 661.3 0 387 8 626 661.3 0 626 9 23559 661.3 0 23559 10 96 661.3 0 96 11 25 661.3 0 25 12 20 661.3 0 20 13 16 661.3 0 16 14 22 661.3 0 22 15 17 661.3 0 17 16 16 661.3 0 16 17 28 661.3 0 28 18 30 661.3 0 30 19 27 661.3 0 27 20 18 661.3 0 18 21 23 661.3 0 23 22 23 661.3 0 23 23 24 661.3 0 24 24 22 661.3 0 22 25 39 661.3 0 39 26 20 661.3 0 20 27 28 661.3 0 28 28 16 661.3 0 16 29 33 661.3 0 33 30 18 661.3 0 18 31 17 661.3 0 17 32 116 661.3 0 116 33 33 661.3 0 33 34 43 661.3 0 43 35 41 661.3 0 41 36 34 661.3 0 34 37 161 661.3 0 161 38 59 661.3 0 59 39 97 661.3 0 97 40 55 661.3 0 55 41 324 661.3 0 324 42 219 661.3 0 219 43 17 661.3 0 17 44 15 661.3 0 15 45 10 661.3 0 10 46 21 661.3 0 21 47 19 661.3 0 19 48 24 661.3 0 24 49 48 661.3 0 48 50 18 661.3 0 18 51 38 661.3 0 38 52 293 661.3 0 293 53 44 661.3 0 44 54 74 661.3 0 74 55 20 661.3 0 20 56 11 661.3 0 11 57 18 661.3 0 18 58 20 661.3 0 20 59 19 661.3 0 19 60 43 661.3 0 43 61 51 661.3 0 51 62 42 661.3 0 42 63 10 661.3 0 10 64 12 661.3 0 12 65 30 661.3 0 30 66 20 661.3 0 20 67 21 661.3 0 21 68 39 661.3 0 39 69 10 661.3 0 10 70 12 661.3 0 12 71 30 661.3 0 30 72 223 661.3 0 223 73 297 661.3 0 297 74 10 661.3 0 10 75 13 661.3 0 13 76 12 661.3 0 12 77 15 661.3 0 15 78 12 661.3 0 12 79 11 661.3 0 11 80 15 661.3 0 15 81 28 661.3 0 28 82 24 661.3 0 24 83 27 661.3 0 27 84 137 661.3 0 137 85 75 661.3 0 75 86 90 661.3 0 90 87 24 661.3 0 24 88 13 661.3 0 13 89 19 661.3 0 19 90 33 661.3 0 33 91 29 661.3 0 29 92 62 661.3 0 62 93 112 661.3 0 112 94 133 661.3 0 133 95 47 661.3 0 47 96 69 661.3 0 69 97 154 661.3 0 154 98 17 661.3 0 17 99 11 661.3 0 11 100 13 661.3 0 13 101 14 661.3 0 14 102 23 661.3 0 23 103 23 661.3 0 23 104 137 661.3 0 137 105 36 661.3 0 36 106 28 661.3 0 28 107 42 661.3 0 42 108 148 661.3 0 148 109 61 661.3 0 61 110 81 661.3 0 81 111 61 661.3 0 61 112 64 661.3 0 64 113 39 661.3 0 39 114 15 661.3 0 15 115 20 661.3 0 20 116 26 661.3 0 26 117 43 661.3 0 43 118 45 661.3 0 45 119 62 661.3 0 62 120 23 661.3 0 23 121 22 661.3 0 22 122 25 661.3 0 25 123 25 661.3 0 25 124 34 661.3 0 34 125 39 661.3 0 39 126 61 661.3 0 61 127 193 661.3 0 193 128 66 661.3 0 66 129 125 661.3 0 125 130 37 661.3 0 37 131 50 661.3 0 50 132 88 661.3 0 88 133 69 661.3 0 69 134 51 661.3 0 51 135 20 661.3 0 20 136 33 661.3 0 33 137 51 661.3 0 51 138 73 661.3 0 73 139 161 661.3 0 161 140 116 661.3 0 116 141 150 661.3 0 150 142 268 661.3 0 268 143 409 661.3 0 409 144 6924 661.3 0 6924 145 2564 661.3 0 2564 146 6573 661.3 0 6573 147 72 661.3 0 72 148 24 661.3 0 24 149 26 661.3 0 26 150 31 661.3 0 31 151 171 661.3 0 171 152 101 661.3 0 101 153 106 661.3 0 106 154 101 661.3 0 101 155 43 661.3 0 43 156 91 661.3 0 91 157 136 661.3 0 136 158 97 661.3 0 97 159 174 661.3 0 174 160 25 661.3 0 25 161 32 661.3 0 32 162 16 661.3 0 16 163 20 661.3 0 20 164 24 661.3 0 24 165 40 661.3 0 40 166 32 661.3 0 32 167 28 661.3 0 28 168 18 661.3 0 18 169 27 661.3 0 27 170 24 661.3 0 24 171 74 661.3 0 74 172 76 661.3 0 76 173 53 661.3 0 53 174 36 661.3 0 36 175 28 661.3 0 28 176 38 661.3 0 38 177 53 661.3 0 53 178 89 661.3 0 89 179 559 661.3 0 559 180 109 661.3 0 109 181 205 661.3 0 205 182 34 661.3 0 34 183 37 661.3 0 37 184 28 661.3 0 28 185 43 661.3 0 43 186 32 661.3 0 32 187 27 661.3 0 27 188 17 661.3 0 17 189 25 661.3 0 25 190 129 661.3 0 129 191 60 661.3 0 60 192 97 661.3 0 97 193 37 661.3 0 37 194 34 661.3 0 34 195 14 661.3 0 14 196 59 661.3 0 59 197 68 661.3 0 68 198 185 661.3 0 185 199 69 661.3 0 69 200 32 661.3 0 32 201 27 661.3 0 27 202 21 661.3 0 21 203 17 661.3 0 17 204 31 661.3 0 31 205 32 661.3 0 32 206 38 661.3 0 38 207 27 661.3 0 27 208 20 661.3 0 20 209 19 661.3 0 19 210 21 661.3 0 21 211 18 661.3 0 18 212 28 661.3 0 28 213 28 661.3 0 28 214 23 661.3 0 23 215 24 661.3 0 24 216 32 661.3 0 32 217 56 661.3 0 56 218 73 661.3 0 73 219 33 661.3 0 33 220 19 661.3 0 19 221 25 661.3 0 25 222 17 661.3 0 17 223 25 661.3 0 25 224 23 661.3 0 23 225 26 661.3 0 26 226 42 661.3 0 42 227 44 661.3 0 44 228 67 661.3 0 67 229 41 661.3 0 41 230 84 661.3 0 84 231 62 661.3 0 62 232 52 661.3 0 52 233 49 661.3 0 49 234 59 661.3 0 59 235 215 661.3 0 215 236 349 661.3 0 349 237 183 661.3 0 183 238 171 661.3 0 171 239 33 661.3 0 33 240 199 661.3 0 199 241 74 661.3 0 74 242 129 661.3 0 129 243 25 661.3 0 25 244 19 661.3 0 19 245 43 661.3 0 43 246 47 661.3 0 47 247 84 661.3 0 84 248 47 661.3 0 47 249 68 661.3 0 68 250 40 661.3 0 40 251 62 661.3 0 62 252 122 661.3 0 122 253 65 661.3 0 65 254 53 661.3 0 53 255 62 661.3 0 62 256 46 661.3 0 46 257 30 661.3 0 30 258 29 661.3 0 29 259 20 661.3 0 20 260 317 661.3 0 317 261 395 661.3 0 395 262 771 661.3 0 771 263 485 661.3 0 485 264 35 661.3 0 35 265 46 661.3 0 46 266 31 661.3 0 31 267 56 661.3 0 56 268 49 661.3 0 49 269 20 661.3 0 20 270 32 661.3 0 32 271 101 661.3 0 101 272 102 661.3 0 102 273 23 661.3 0 23 274 13 661.3 0 13 275 22 661.3 0 22 276 32 661.3 0 32 277 54 661.3 0 54 278 35 661.3 0 35 279 27 661.3 0 27 280 29 661.3 0 29 281 32 661.3 0 32 282 40 661.3 0 40 283 141 661.3 0 141 284 158 661.3 0 158 285 121 661.3 0 121 286 69 661.3 0 69 287 44 661.3 0 44 288 104 661.3 0 104 289 37 661.3 0 37 290 46 661.3 0 46 291 144 661.3 0 144 292 108 661.3 0 108 293 156 661.3 0 156 294 124 661.3 0 124 295 38 661.3 0 38 296 39 661.3 0 39 297 23 661.3 0 23 298 17 661.3 0 17 299 15 661.3 0 15 300 8 661.3 0 8 301 19 661.3 0 19 Finished in 2.31 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 52,704 Reads with adapters: 25,001 (47.4%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 24,999 (47.4%) Total basepairs processed: 11,983,153 bp Total written (filtered): 6,801,155 bp (56.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25001 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 14 0.0 3 0 0 13 1 19 394 0.0 3 0 365 19 5 5 20 24190 0.0 4 22970 1065 99 37 19 21 57 0.0 4 4 51 1 1 22 11 0.0 4 2 4 4 1 23 320 0.0 4 295 7 2 11 5 24 4 0.0 4 0 1 0 0 3 27 1 0.0 4 1 40 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 49 1 0.0 4 1 68 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 24,999 Reads with adapters: 1,861 (7.4%) Reads written (passing filters): 24,999 (100.0%) Total basepairs processed: 6,801,155 bp Total written (filtered): 6,639,028 bp (97.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1861 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 18.0% C: 3.3% G: 75.6% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 390.6 0 9 6 45 6.1 0 45 9 185 0.1 0 24 161 10 696 0.0 1 31 665 11 342 0.0 1 2 340 12 3 0.0 1 0 3 13 1 0.0 1 0 1 150 7 0.0 1 4 3 208 14 0.0 1 14 218 1 0.0 1 1 223 6 0.0 1 6 224 2 0.0 1 2 225 1 0.0 1 1 240 11 0.0 1 11 256 1 0.0 1 1 257 247 0.0 1 239 8 258 10 0.0 1 10 259 8 0.0 1 8 260 1 0.0 1 1 261 2 0.0 1 2 263 1 0.0 1 1 264 1 0.0 1 1 265 230 0.0 1 220 10 266 11 0.0 1 11 267 1 0.0 1 1 268 4 0.0 1 4 269 19 0.0 1 19 270 1 0.0 1 1 271 1 0.0 1 1