This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.06 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 254,342 (9.4%) Reads that were too short: 13,524 (0.5%) Reads written (passing filters): 240,818 (8.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 68,205,935 bp (8.4%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 254342 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1205 661.3 0 1205 7 1650 661.3 0 1650 8 29451 661.3 0 29451 9 188294 661.3 0 188294 10 328 661.3 0 328 11 62 661.3 0 62 12 52 661.3 0 52 13 43 661.3 0 43 14 31 661.3 0 31 15 37 661.3 0 37 16 28 661.3 0 28 17 46 661.3 0 46 18 39 661.3 0 39 19 51 661.3 0 51 20 42 661.3 0 42 21 28 661.3 0 28 22 37 661.3 0 37 23 30 661.3 0 30 24 50 661.3 0 50 25 48 661.3 0 48 26 28 661.3 0 28 27 31 661.3 0 31 28 35 661.3 0 35 29 42 661.3 0 42 30 38 661.3 0 38 31 35 661.3 0 35 32 25 661.3 0 25 33 32 661.3 0 32 34 36 661.3 0 36 35 39 661.3 0 39 36 33 661.3 0 33 37 31 661.3 0 31 38 40 661.3 0 40 39 44 661.3 0 44 40 79 661.3 0 79 41 17 661.3 0 17 42 32 661.3 0 32 43 38 661.3 0 38 44 58 661.3 0 58 45 50 661.3 0 50 46 36 661.3 0 36 47 42 661.3 0 42 48 39 661.3 0 39 49 399 661.3 0 399 50 27 661.3 0 27 51 122 661.3 0 122 52 93 661.3 0 93 53 41 661.3 0 41 54 28 661.3 0 28 55 32 661.3 0 32 56 30 661.3 0 30 57 39 661.3 0 39 58 40 661.3 0 40 59 32 661.3 0 32 60 18 661.3 0 18 61 42 661.3 0 42 62 45 661.3 0 45 63 49 661.3 0 49 64 51 661.3 0 51 65 49 661.3 0 49 66 37 661.3 0 37 67 54 661.3 0 54 68 31 661.3 0 31 69 48 661.3 0 48 70 54 661.3 0 54 71 96 661.3 0 96 72 49 661.3 0 49 73 45 661.3 0 45 74 46 661.3 0 46 75 38 661.3 0 38 76 53 661.3 0 53 77 68 661.3 0 68 78 69 661.3 0 69 79 110 661.3 0 110 80 727 661.3 0 727 81 289 661.3 0 289 82 324 661.3 0 324 83 91 661.3 0 91 84 37 661.3 0 37 85 76 661.3 0 76 86 77 661.3 0 77 87 61 661.3 0 61 88 41 661.3 0 41 89 64 661.3 0 64 90 52 661.3 0 52 91 63 661.3 0 63 92 57 661.3 0 57 93 80 661.3 0 80 94 80 661.3 0 80 95 93 661.3 0 93 96 923 661.3 0 923 97 1207 661.3 0 1207 98 2241 661.3 0 2241 99 85 661.3 0 85 100 50 661.3 0 50 101 45 661.3 0 45 102 48 661.3 0 48 103 38 661.3 0 38 104 38 661.3 0 38 105 39 661.3 0 39 106 48 661.3 0 48 107 40 661.3 0 40 108 60 661.3 0 60 109 86 661.3 0 86 110 43 661.3 0 43 111 67 661.3 0 67 112 44 661.3 0 44 113 65 661.3 0 65 114 83 661.3 0 83 115 51 661.3 0 51 116 46 661.3 0 46 117 49 661.3 0 49 118 68 661.3 0 68 119 59 661.3 0 59 120 57 661.3 0 57 121 53 661.3 0 53 122 47 661.3 0 47 123 51 661.3 0 51 124 58 661.3 0 58 125 45 661.3 0 45 126 43 661.3 0 43 127 70 661.3 0 70 128 143 661.3 0 143 129 87 661.3 0 87 130 158 661.3 0 158 131 78 661.3 0 78 132 321 661.3 0 321 133 113 661.3 0 113 134 194 661.3 0 194 135 79 661.3 0 79 136 63 661.3 0 63 137 69 661.3 0 69 138 112 661.3 0 112 139 69 661.3 0 69 140 68 661.3 0 68 141 69 661.3 0 69 142 57 661.3 0 57 143 65 661.3 0 65 144 66 661.3 0 66 145 56 661.3 0 56 146 60 661.3 0 60 147 60 661.3 0 60 148 226 661.3 0 226 149 705 661.3 0 705 150 244 661.3 0 244 151 41 661.3 0 41 152 40 661.3 0 40 153 56 661.3 0 56 154 139 661.3 0 139 155 58 661.3 0 58 156 49 661.3 0 49 157 64 661.3 0 64 158 71 661.3 0 71 159 40 661.3 0 40 160 50 661.3 0 50 161 56 661.3 0 56 162 52 661.3 0 52 163 47 661.3 0 47 164 60 661.3 0 60 165 57 661.3 0 57 166 58 661.3 0 58 167 60 661.3 0 60 168 41 661.3 0 41 169 54 661.3 0 54 170 71 661.3 0 71 171 54 661.3 0 54 172 62 661.3 0 62 173 53 661.3 0 53 174 57 661.3 0 57 175 62 661.3 0 62 176 73 661.3 0 73 177 60 661.3 0 60 178 50 661.3 0 50 179 66 661.3 0 66 180 75 661.3 0 75 181 80 661.3 0 80 182 65 661.3 0 65 183 57 661.3 0 57 184 101 661.3 0 101 185 107 661.3 0 107 186 95 661.3 0 95 187 272 661.3 0 272 188 458 661.3 0 458 189 827 661.3 0 827 190 556 661.3 0 556 191 98 661.3 0 98 192 63 661.3 0 63 193 61 661.3 0 61 194 65 661.3 0 65 195 57 661.3 0 57 196 100 661.3 0 100 197 57 661.3 0 57 198 54 661.3 0 54 199 44 661.3 0 44 200 53 661.3 0 53 201 84 661.3 0 84 202 60 661.3 0 60 203 71 661.3 0 71 204 70 661.3 0 70 205 83 661.3 0 83 206 89 661.3 0 89 207 93 661.3 0 93 208 98 661.3 0 98 209 285 661.3 0 285 210 313 661.3 0 313 211 356 661.3 0 356 212 178 661.3 0 178 213 82 661.3 0 82 214 73 661.3 0 73 215 88 661.3 0 88 216 59 661.3 0 59 217 70 661.3 0 70 218 61 661.3 0 61 219 95 661.3 0 95 220 80 661.3 0 80 221 89 661.3 0 89 222 102 661.3 0 102 223 90 661.3 0 90 224 88 661.3 0 88 225 108 661.3 0 108 226 131 661.3 0 131 227 97 661.3 0 97 228 122 661.3 0 122 229 80 661.3 0 80 230 108 661.3 0 108 231 89 661.3 0 89 232 138 661.3 0 138 233 127 661.3 0 127 234 198 661.3 0 198 235 173 661.3 0 173 236 156 661.3 0 156 237 147 661.3 0 147 238 148 661.3 0 148 239 99 661.3 0 99 240 211 661.3 0 211 241 404 661.3 0 404 242 674 661.3 0 674 243 160 661.3 0 160 244 154 661.3 0 154 245 112 661.3 0 112 246 82 661.3 0 82 247 86 661.3 0 86 248 83 661.3 0 83 249 257 661.3 0 257 250 445 661.3 0 445 251 832 661.3 0 832 252 95 661.3 0 95 253 281 661.3 0 281 254 510 661.3 0 510 255 298 661.3 0 298 256 152 661.3 0 152 257 112 661.3 0 112 258 77 661.3 0 77 259 71 661.3 0 71 260 165 661.3 0 165 261 215 661.3 0 215 262 192 661.3 0 192 263 83 661.3 0 83 264 81 661.3 0 81 265 84 661.3 0 84 266 81 661.3 0 81 267 36 661.3 0 36 268 120 661.3 0 120 269 128 661.3 0 128 270 175 661.3 0 175 271 178 661.3 0 178 272 114 661.3 0 114 273 87 661.3 0 87 274 123 661.3 0 123 275 93 661.3 0 93 276 65 661.3 0 65 277 84 661.3 0 84 278 78 661.3 0 78 279 61 661.3 0 61 280 65 661.3 0 65 281 100 661.3 0 100 282 133 661.3 0 133 283 77 661.3 0 77 284 93 661.3 0 93 285 92 661.3 0 92 286 59 661.3 0 59 287 38 661.3 0 38 288 69 661.3 0 69 289 87 661.3 0 87 290 73 661.3 0 73 291 47 661.3 0 47 292 68 661.3 0 68 293 52 661.3 0 52 294 48 661.3 0 48 295 52 661.3 0 52 296 56 661.3 0 56 297 55 661.3 0 55 298 70 661.3 0 70 299 66 661.3 0 66 300 81 661.3 0 81 301 110 661.3 0 110 Finished in 10.69 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 240,818 Reads with adapters: 24,788 (10.3%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 24,764 (10.3%) Total basepairs processed: 68,205,935 bp Total written (filtered): 6,832,137 bp (10.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24788 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 16 0.0 3 0 0 14 2 16 252 0.0 3 0 248 4 17 24290 0.0 3 24012 269 8 1 18 66 0.0 3 3 54 6 3 19 17 0.0 3 1 1 13 2 20 96 0.0 3 68 5 0 23 22 1 0.0 3 0 0 1 30 1 0.0 3 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 70 5 0.0 3 0 0 0 5 73 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 0 5 172 1 0.0 3 1 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 1 282 2 0.0 3 0 0 0 2 283 3 0.0 3 0 0 0 3 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 24,764 Reads with adapters: 183 (0.7%) Reads written (passing filters): 24,764 (100.0%) Total basepairs processed: 6,832,137 bp Total written (filtered): 6,831,588 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 183 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.0% C: 27.3% G: 31.1% T: 6.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 183 386.9 0 183