This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.86 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 47,372 (1.7%) Reads that were too short: 13,368 (0.5%) Reads written (passing filters): 34,004 (1.3%) Total basepairs processed: 815,312,680 bp Total written (filtered): 7,039,269 bp (0.9%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 47372 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 158 661.3 0 158 7 161 661.3 0 161 8 6358 661.3 0 6358 9 78 661.3 0 78 10 36 661.3 0 36 11 23 661.3 0 23 12 40 661.3 0 40 13 30 661.3 0 30 14 28 661.3 0 28 15 25 661.3 0 25 16 30 661.3 0 30 17 25 661.3 0 25 18 60 661.3 0 60 19 43 661.3 0 43 20 64 661.3 0 64 21 23 661.3 0 23 22 52 661.3 0 52 23 32 661.3 0 32 24 31 661.3 0 31 25 40 661.3 0 40 26 50 661.3 0 50 27 45 661.3 0 45 28 39 661.3 0 39 29 38 661.3 0 38 30 39 661.3 0 39 31 42 661.3 0 42 32 35 661.3 0 35 33 39 661.3 0 39 34 42 661.3 0 42 35 41 661.3 0 41 36 32 661.3 0 32 37 33 661.3 0 33 38 32 661.3 0 32 39 37 661.3 0 37 40 38 661.3 0 38 41 46 661.3 0 46 42 27 661.3 0 27 43 38 661.3 0 38 44 56 661.3 0 56 45 27 661.3 0 27 46 41 661.3 0 41 47 46 661.3 0 46 48 50 661.3 0 50 49 33 661.3 0 33 50 27 661.3 0 27 51 51 661.3 0 51 52 41 661.3 0 41 53 37 661.3 0 37 54 62 661.3 0 62 55 46 661.3 0 46 56 40 661.3 0 40 57 37 661.3 0 37 58 31 661.3 0 31 59 59 661.3 0 59 60 45 661.3 0 45 61 42 661.3 0 42 62 51 661.3 0 51 63 32 661.3 0 32 64 44 661.3 0 44 65 46 661.3 0 46 66 56 661.3 0 56 67 95 661.3 0 95 68 76 661.3 0 76 69 151 661.3 0 151 70 217 661.3 0 217 71 3313 661.3 0 3313 72 1113 661.3 0 1113 73 1921 661.3 0 1921 74 67 661.3 0 67 75 54 661.3 0 54 76 57 661.3 0 57 77 124 661.3 0 124 78 114 661.3 0 114 79 88 661.3 0 88 80 74 661.3 0 74 81 62 661.3 0 62 82 56 661.3 0 56 83 73 661.3 0 73 84 103 661.3 0 103 85 125 661.3 0 125 86 191 661.3 0 191 87 115 661.3 0 115 88 64 661.3 0 64 89 55 661.3 0 55 90 115 661.3 0 115 91 110 661.3 0 110 92 101 661.3 0 101 93 386 661.3 0 386 94 257 661.3 0 257 95 191 661.3 0 191 96 810 661.3 0 810 97 403 661.3 0 403 98 670 661.3 0 670 99 114 661.3 0 114 100 52 661.3 0 52 101 45 661.3 0 45 102 36 661.3 0 36 103 42 661.3 0 42 104 37 661.3 0 37 105 40 661.3 0 40 106 52 661.3 0 52 107 45 661.3 0 45 108 105 661.3 0 105 109 64 661.3 0 64 110 48 661.3 0 48 111 52 661.3 0 52 112 71 661.3 0 71 113 74 661.3 0 74 114 141 661.3 0 141 115 125 661.3 0 125 116 92 661.3 0 92 117 85 661.3 0 85 118 95 661.3 0 95 119 100 661.3 0 100 120 98 661.3 0 98 121 95 661.3 0 95 122 90 661.3 0 90 123 97 661.3 0 97 124 407 661.3 0 407 125 131 661.3 0 131 126 234 661.3 0 234 127 151 661.3 0 151 128 136 661.3 0 136 129 140 661.3 0 140 130 146 661.3 0 146 131 131 661.3 0 131 132 117 661.3 0 117 133 111 661.3 0 111 134 121 661.3 0 121 135 143 661.3 0 143 136 155 661.3 0 155 137 117 661.3 0 117 138 96 661.3 0 96 139 91 661.3 0 91 140 115 661.3 0 115 141 105 661.3 0 105 142 84 661.3 0 84 143 143 661.3 0 143 144 139 661.3 0 139 145 117 661.3 0 117 146 105 661.3 0 105 147 80 661.3 0 80 148 73 661.3 0 73 149 93 661.3 0 93 150 136 661.3 0 136 151 101 661.3 0 101 152 88 661.3 0 88 153 139 661.3 0 139 154 183 661.3 0 183 155 183 661.3 0 183 156 158 661.3 0 158 157 105 661.3 0 105 158 113 661.3 0 113 159 108 661.3 0 108 160 95 661.3 0 95 161 99 661.3 0 99 162 93 661.3 0 93 163 100 661.3 0 100 164 133 661.3 0 133 165 171 661.3 0 171 166 157 661.3 0 157 167 112 661.3 0 112 168 97 661.3 0 97 169 93 661.3 0 93 170 88 661.3 0 88 171 119 661.3 0 119 172 160 661.3 0 160 173 234 661.3 0 234 174 190 661.3 0 190 175 214 661.3 0 214 176 111 661.3 0 111 177 111 661.3 0 111 178 146 661.3 0 146 179 138 661.3 0 138 180 154 661.3 0 154 181 147 661.3 0 147 182 116 661.3 0 116 183 124 661.3 0 124 184 126 661.3 0 126 185 142 661.3 0 142 186 102 661.3 0 102 187 106 661.3 0 106 188 84 661.3 0 84 189 123 661.3 0 123 190 188 661.3 0 188 191 288 661.3 0 288 192 790 661.3 0 790 193 415 661.3 0 415 194 566 661.3 0 566 195 188 661.3 0 188 196 119 661.3 0 119 197 97 661.3 0 97 198 120 661.3 0 120 199 77 661.3 0 77 200 89 661.3 0 89 201 111 661.3 0 111 202 129 661.3 0 129 203 208 661.3 0 208 204 294 661.3 0 294 205 148 661.3 0 148 206 105 661.3 0 105 207 181 661.3 0 181 208 96 661.3 0 96 209 115 661.3 0 115 210 214 661.3 0 214 211 228 661.3 0 228 212 162 661.3 0 162 213 112 661.3 0 112 214 100 661.3 0 100 215 111 661.3 0 111 216 137 661.3 0 137 217 184 661.3 0 184 218 116 661.3 0 116 219 106 661.3 0 106 220 107 661.3 0 107 221 181 661.3 0 181 222 140 661.3 0 140 223 89 661.3 0 89 224 117 661.3 0 117 225 124 661.3 0 124 226 158 661.3 0 158 227 110 661.3 0 110 228 95 661.3 0 95 229 87 661.3 0 87 230 83 661.3 0 83 231 78 661.3 0 78 232 112 661.3 0 112 233 116 661.3 0 116 234 131 661.3 0 131 235 119 661.3 0 119 236 111 661.3 0 111 237 115 661.3 0 115 238 110 661.3 0 110 239 75 661.3 0 75 240 136 661.3 0 136 241 140 661.3 0 140 242 159 661.3 0 159 243 108 661.3 0 108 244 114 661.3 0 114 245 121 661.3 0 121 246 103 661.3 0 103 247 120 661.3 0 120 248 54 661.3 0 54 249 102 661.3 0 102 250 109 661.3 0 109 251 169 661.3 0 169 252 113 661.3 0 113 253 226 661.3 0 226 254 192 661.3 0 192 255 186 661.3 0 186 256 125 661.3 0 125 257 61 661.3 0 61 258 49 661.3 0 49 259 87 661.3 0 87 260 110 661.3 0 110 261 118 661.3 0 118 262 133 661.3 0 133 263 142 661.3 0 142 264 141 661.3 0 141 265 179 661.3 0 179 266 102 661.3 0 102 267 78 661.3 0 78 268 68 661.3 0 68 269 90 661.3 0 90 270 152 661.3 0 152 271 187 661.3 0 187 272 375 661.3 0 375 273 250 661.3 0 250 274 109 661.3 0 109 275 81 661.3 0 81 276 165 661.3 0 165 277 140 661.3 0 140 278 162 661.3 0 162 279 95 661.3 0 95 280 85 661.3 0 85 281 82 661.3 0 82 282 98 661.3 0 98 283 101 661.3 0 101 284 136 661.3 0 136 285 112 661.3 0 112 286 97 661.3 0 97 287 88 661.3 0 88 288 146 661.3 0 146 289 153 661.3 0 153 290 271 661.3 0 271 291 260 661.3 0 260 292 175 661.3 0 175 293 134 661.3 0 134 294 109 661.3 0 109 295 127 661.3 0 127 296 183 661.3 0 183 297 120 661.3 0 120 298 125 661.3 0 125 299 90 661.3 0 90 300 189 661.3 0 189 301 132 661.3 0 132 Finished in 1.45 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 34,004 Reads with adapters: 6,521 (19.2%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 6,513 (19.2%) Total basepairs processed: 7,039,269 bp Total written (filtered): 1,778,205 bp (25.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6521 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 115 0.0 3 0 106 5 1 3 20 6361 0.0 4 6229 112 17 2 1 21 20 0.0 4 3 17 22 3 0.0 4 0 1 2 23 8 0.0 4 0 0 0 8 24 3 0.0 4 2 0 0 0 1 47 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 6,513 Reads with adapters: 1,036 (15.9%) Reads written (passing filters): 6,513 (100.0%) Total basepairs processed: 1,778,205 bp Total written (filtered): 1,699,630 bp (95.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1036 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 27.0% C: 1.6% G: 70.4% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 0 1 10 27 0.0 1 0 27 11 735 0.0 1 2 733 12 2 0.0 1 0 2 153 1 0.0 1 0 1 209 2 0.0 1 0 2 258 2 0.0 1 2 259 196 0.0 1 185 11 260 5 0.0 1 5 261 3 0.0 1 3 262 60 0.0 1 55 5 263 1 0.0 1 1 270 1 0.0 1 1