This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.10 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 32,151 (1.2%) Reads that were too short: 3,611 (0.1%) Reads written (passing filters): 28,540 (1.1%) Total basepairs processed: 815,312,680 bp Total written (filtered): 7,528,409 bp (0.9%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 32151 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 331 661.3 0 331 7 7037 661.3 0 7037 8 11857 661.3 0 11857 9 49 661.3 0 49 10 28 661.3 0 28 11 39 661.3 0 39 12 38 661.3 0 38 13 30 661.3 0 30 14 40 661.3 0 40 15 31 661.3 0 31 16 37 661.3 0 37 17 39 661.3 0 39 18 30 661.3 0 30 19 39 661.3 0 39 20 34 661.3 0 34 21 35 661.3 0 35 22 35 661.3 0 35 23 30 661.3 0 30 24 40 661.3 0 40 25 29 661.3 0 29 26 30 661.3 0 30 27 20 661.3 0 20 28 28 661.3 0 28 29 25 661.3 0 25 30 39 661.3 0 39 31 41 661.3 0 41 32 31 661.3 0 31 33 31 661.3 0 31 34 21 661.3 0 21 35 33 661.3 0 33 36 47 661.3 0 47 37 25 661.3 0 25 38 39 661.3 0 39 39 38 661.3 0 38 40 39 661.3 0 39 41 31 661.3 0 31 42 34 661.3 0 34 43 49 661.3 0 49 44 29 661.3 0 29 45 35 661.3 0 35 46 37 661.3 0 37 47 22 661.3 0 22 48 42 661.3 0 42 49 42 661.3 0 42 50 44 661.3 0 44 51 98 661.3 0 98 52 69 661.3 0 69 53 40 661.3 0 40 54 43 661.3 0 43 55 33 661.3 0 33 56 55 661.3 0 55 57 43 661.3 0 43 58 30 661.3 0 30 59 41 661.3 0 41 60 38 661.3 0 38 61 42 661.3 0 42 62 37 661.3 0 37 63 21 661.3 0 21 64 42 661.3 0 42 65 36 661.3 0 36 66 40 661.3 0 40 67 43 661.3 0 43 68 54 661.3 0 54 69 273 661.3 0 273 70 106 661.3 0 106 71 319 661.3 0 319 72 697 661.3 0 697 73 289 661.3 0 289 74 224 661.3 0 224 75 50 661.3 0 50 76 21 661.3 0 21 77 20 661.3 0 20 78 27 661.3 0 27 79 33 661.3 0 33 80 35 661.3 0 35 81 58 661.3 0 58 82 54 661.3 0 54 83 65 661.3 0 65 84 391 661.3 0 391 85 87 661.3 0 87 86 168 661.3 0 168 87 26 661.3 0 26 88 30 661.3 0 30 89 30 661.3 0 30 90 7 661.3 0 7 91 21 661.3 0 21 92 25 661.3 0 25 93 35 661.3 0 35 94 47 661.3 0 47 95 31 661.3 0 31 96 21 661.3 0 21 97 35 661.3 0 35 98 27 661.3 0 27 99 41 661.3 0 41 100 38 661.3 0 38 101 28 661.3 0 28 102 34 661.3 0 34 103 21 661.3 0 21 104 13 661.3 0 13 105 29 661.3 0 29 106 27 661.3 0 27 107 37 661.3 0 37 108 31 661.3 0 31 109 30 661.3 0 30 110 41 661.3 0 41 111 263 661.3 0 263 112 31 661.3 0 31 113 27 661.3 0 27 114 24 661.3 0 24 115 19 661.3 0 19 116 22 661.3 0 22 117 21 661.3 0 21 118 24 661.3 0 24 119 19 661.3 0 19 120 41 661.3 0 41 121 24 661.3 0 24 122 21 661.3 0 21 123 17 661.3 0 17 124 38 661.3 0 38 125 51 661.3 0 51 126 36 661.3 0 36 127 28 661.3 0 28 128 31 661.3 0 31 129 30 661.3 0 30 130 20 661.3 0 20 131 140 661.3 0 140 132 28 661.3 0 28 133 18 661.3 0 18 134 22 661.3 0 22 135 34 661.3 0 34 136 31 661.3 0 31 137 21 661.3 0 21 138 34 661.3 0 34 139 33 661.3 0 33 140 25 661.3 0 25 141 19 661.3 0 19 142 33 661.3 0 33 143 22 661.3 0 22 144 22 661.3 0 22 145 22 661.3 0 22 146 28 661.3 0 28 147 40 661.3 0 40 148 97 661.3 0 97 149 52 661.3 0 52 150 56 661.3 0 56 151 32 661.3 0 32 152 24 661.3 0 24 153 28 661.3 0 28 154 27 661.3 0 27 155 29 661.3 0 29 156 40 661.3 0 40 157 29 661.3 0 29 158 35 661.3 0 35 159 23 661.3 0 23 160 13 661.3 0 13 161 31 661.3 0 31 162 26 661.3 0 26 163 23 661.3 0 23 164 20 661.3 0 20 165 19 661.3 0 19 166 23 661.3 0 23 167 32 661.3 0 32 168 78 661.3 0 78 169 45 661.3 0 45 170 34 661.3 0 34 171 29 661.3 0 29 172 32 661.3 0 32 173 26 661.3 0 26 174 35 661.3 0 35 175 24 661.3 0 24 176 97 661.3 0 97 177 35 661.3 0 35 178 66 661.3 0 66 179 25 661.3 0 25 180 33 661.3 0 33 181 22 661.3 0 22 182 71 661.3 0 71 183 40 661.3 0 40 184 34 661.3 0 34 185 18 661.3 0 18 186 32 661.3 0 32 187 31 661.3 0 31 188 34 661.3 0 34 189 27 661.3 0 27 190 27 661.3 0 27 191 29 661.3 0 29 192 24 661.3 0 24 193 28 661.3 0 28 194 26 661.3 0 26 195 53 661.3 0 53 196 56 661.3 0 56 197 62 661.3 0 62 198 111 661.3 0 111 199 82 661.3 0 82 200 23 661.3 0 23 201 26 661.3 0 26 202 40 661.3 0 40 203 97 661.3 0 97 204 143 661.3 0 143 205 86 661.3 0 86 206 53 661.3 0 53 207 32 661.3 0 32 208 23 661.3 0 23 209 21 661.3 0 21 210 34 661.3 0 34 211 28 661.3 0 28 212 25 661.3 0 25 213 69 661.3 0 69 214 79 661.3 0 79 215 77 661.3 0 77 216 20 661.3 0 20 217 41 661.3 0 41 218 28 661.3 0 28 219 30 661.3 0 30 220 22 661.3 0 22 221 34 661.3 0 34 222 53 661.3 0 53 223 86 661.3 0 86 224 190 661.3 0 190 225 137 661.3 0 137 226 22 661.3 0 22 227 33 661.3 0 33 228 28 661.3 0 28 229 13 661.3 0 13 230 15 661.3 0 15 231 20 661.3 0 20 232 18 661.3 0 18 233 27 661.3 0 27 234 22 661.3 0 22 235 22 661.3 0 22 236 31 661.3 0 31 237 23 661.3 0 23 238 29 661.3 0 29 239 39 661.3 0 39 240 32 661.3 0 32 241 30 661.3 0 30 242 33 661.3 0 33 243 17 661.3 0 17 244 41 661.3 0 41 245 26 661.3 0 26 246 29 661.3 0 29 247 28 661.3 0 28 248 28 661.3 0 28 249 24 661.3 0 24 250 32 661.3 0 32 251 17 661.3 0 17 252 18 661.3 0 18 253 26 661.3 0 26 254 30 661.3 0 30 255 48 661.3 0 48 256 27 661.3 0 27 257 38 661.3 0 38 258 51 661.3 0 51 259 86 661.3 0 86 260 58 661.3 0 58 261 50 661.3 0 50 262 34 661.3 0 34 263 20 661.3 0 20 264 26 661.3 0 26 265 22 661.3 0 22 266 10 661.3 0 10 267 24 661.3 0 24 268 17 661.3 0 17 269 27 661.3 0 27 270 32 661.3 0 32 271 33 661.3 0 33 272 32 661.3 0 32 273 30 661.3 0 30 274 22 661.3 0 22 275 24 661.3 0 24 276 25 661.3 0 25 277 25 661.3 0 25 278 32 661.3 0 32 279 30 661.3 0 30 280 20 661.3 0 20 281 34 661.3 0 34 282 29 661.3 0 29 283 33 661.3 0 33 284 28 661.3 0 28 285 31 661.3 0 31 286 31 661.3 0 31 287 19 661.3 0 19 288 18 661.3 0 18 289 22 661.3 0 22 290 18 661.3 0 18 291 26 661.3 0 26 292 22 661.3 0 22 293 19 661.3 0 19 294 25 661.3 0 25 295 17 661.3 0 17 296 26 661.3 0 26 297 24 661.3 0 24 298 45 661.3 0 45 299 44 661.3 0 44 300 30 661.3 0 30 301 26 661.3 0 26 Finished in 1.29 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 28,540 Reads with adapters: 6,545 (22.9%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 6,540 (22.9%) Total basepairs processed: 7,528,409 bp Total written (filtered): 1,810,575 bp (24.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6545 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 51 0.0 3 0 51 17 6453 0.0 3 6364 86 3 18 10 0.0 3 0 9 1 19 4 0.0 3 0 0 4 20 15 0.0 3 0 0 0 15 23 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 6,540 Reads with adapters: 472 (7.2%) Reads written (passing filters): 6,540 (100.0%) Total basepairs processed: 1,810,575 bp Total written (filtered): 1,809,157 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 472 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 4.9% C: 87.1% G: 7.8% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 471 102.2 0 471 5 1 6.4 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.