This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.46 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 128,061 (4.7%) Reads that were too short: 10,672 (0.4%) Reads written (passing filters): 117,389 (4.3%) Total basepairs processed: 815,312,680 bp Total written (filtered): 30,248,500 bp (3.7%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 128061 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1330 661.3 0 1330 7 70896 661.3 0 70896 8 175 661.3 0 175 9 65 661.3 0 65 10 23 661.3 0 23 11 47 661.3 0 47 12 53 661.3 0 53 13 52 661.3 0 52 14 50 661.3 0 50 15 42 661.3 0 42 16 99 661.3 0 99 17 165 661.3 0 165 18 156 661.3 0 156 19 40 661.3 0 40 20 63 661.3 0 63 21 35 661.3 0 35 22 59 661.3 0 59 23 53 661.3 0 53 24 77 661.3 0 77 25 58 661.3 0 58 26 42 661.3 0 42 27 41 661.3 0 41 28 63 661.3 0 63 29 61 661.3 0 61 30 54 661.3 0 54 31 60 661.3 0 60 32 134 661.3 0 134 33 914 661.3 0 914 34 54 661.3 0 54 35 52 661.3 0 52 36 58 661.3 0 58 37 61 661.3 0 61 38 46 661.3 0 46 39 59 661.3 0 59 40 39 661.3 0 39 41 54 661.3 0 54 42 62 661.3 0 62 43 43 661.3 0 43 44 49 661.3 0 49 45 65 661.3 0 65 46 280 661.3 0 280 47 77 661.3 0 77 48 176 661.3 0 176 49 53 661.3 0 53 50 39 661.3 0 39 51 53 661.3 0 53 52 61 661.3 0 61 53 34 661.3 0 34 54 39 661.3 0 39 55 43 661.3 0 43 56 43 661.3 0 43 57 49 661.3 0 49 58 40 661.3 0 40 59 48 661.3 0 48 60 49 661.3 0 49 61 40 661.3 0 40 62 54 661.3 0 54 63 65 661.3 0 65 64 36 661.3 0 36 65 50 661.3 0 50 66 58 661.3 0 58 67 50 661.3 0 50 68 64 661.3 0 64 69 48 661.3 0 48 70 61 661.3 0 61 71 52 661.3 0 52 72 56 661.3 0 56 73 48 661.3 0 48 74 52 661.3 0 52 75 36 661.3 0 36 76 84 661.3 0 84 77 112 661.3 0 112 78 125 661.3 0 125 79 193 661.3 0 193 80 294 661.3 0 294 81 554 661.3 0 554 82 152 661.3 0 152 83 189 661.3 0 189 84 257 661.3 0 257 85 198 661.3 0 198 86 222 661.3 0 222 87 224 661.3 0 224 88 203 661.3 0 203 89 202 661.3 0 202 90 170 661.3 0 170 91 225 661.3 0 225 92 278 661.3 0 278 93 396 661.3 0 396 94 5085 661.3 0 5085 95 5598 661.3 0 5598 96 11221 661.3 0 11221 97 816 661.3 0 816 98 1913 661.3 0 1913 99 79 661.3 0 79 100 71 661.3 0 71 101 92 661.3 0 92 102 62 661.3 0 62 103 46 661.3 0 46 104 42 661.3 0 42 105 34 661.3 0 34 106 33 661.3 0 33 107 50 661.3 0 50 108 76 661.3 0 76 109 94 661.3 0 94 110 292 661.3 0 292 111 239 661.3 0 239 112 205 661.3 0 205 113 107 661.3 0 107 114 68 661.3 0 68 115 85 661.3 0 85 116 90 661.3 0 90 117 87 661.3 0 87 118 102 661.3 0 102 119 105 661.3 0 105 120 69 661.3 0 69 121 82 661.3 0 82 122 74 661.3 0 74 123 52 661.3 0 52 124 184 661.3 0 184 125 124 661.3 0 124 126 453 661.3 0 453 127 128 661.3 0 128 128 140 661.3 0 140 129 100 661.3 0 100 130 142 661.3 0 142 131 183 661.3 0 183 132 207 661.3 0 207 133 137 661.3 0 137 134 103 661.3 0 103 135 87 661.3 0 87 136 84 661.3 0 84 137 281 661.3 0 281 138 176 661.3 0 176 139 231 661.3 0 231 140 101 661.3 0 101 141 124 661.3 0 124 142 94 661.3 0 94 143 148 661.3 0 148 144 116 661.3 0 116 145 130 661.3 0 130 146 127 661.3 0 127 147 99 661.3 0 99 148 90 661.3 0 90 149 95 661.3 0 95 150 69 661.3 0 69 151 67 661.3 0 67 152 66 661.3 0 66 153 54 661.3 0 54 154 69 661.3 0 69 155 79 661.3 0 79 156 91 661.3 0 91 157 102 661.3 0 102 158 207 661.3 0 207 159 142 661.3 0 142 160 102 661.3 0 102 161 95 661.3 0 95 162 59 661.3 0 59 163 71 661.3 0 71 164 70 661.3 0 70 165 74 661.3 0 74 166 106 661.3 0 106 167 95 661.3 0 95 168 86 661.3 0 86 169 54 661.3 0 54 170 68 661.3 0 68 171 71 661.3 0 71 172 67 661.3 0 67 173 70 661.3 0 70 174 76 661.3 0 76 175 99 661.3 0 99 176 79 661.3 0 79 177 87 661.3 0 87 178 65 661.3 0 65 179 90 661.3 0 90 180 90 661.3 0 90 181 86 661.3 0 86 182 85 661.3 0 85 183 88 661.3 0 88 184 99 661.3 0 99 185 108 661.3 0 108 186 109 661.3 0 109 187 109 661.3 0 109 188 72 661.3 0 72 189 53 661.3 0 53 190 72 661.3 0 72 191 89 661.3 0 89 192 186 661.3 0 186 193 153 661.3 0 153 194 624 661.3 0 624 195 75 661.3 0 75 196 51 661.3 0 51 197 54 661.3 0 54 198 94 661.3 0 94 199 103 661.3 0 103 200 65 661.3 0 65 201 60 661.3 0 60 202 71 661.3 0 71 203 111 661.3 0 111 204 101 661.3 0 101 205 421 661.3 0 421 206 219 661.3 0 219 207 202 661.3 0 202 208 67 661.3 0 67 209 83 661.3 0 83 210 99 661.3 0 99 211 89 661.3 0 89 212 113 661.3 0 113 213 123 661.3 0 123 214 78 661.3 0 78 215 75 661.3 0 75 216 75 661.3 0 75 217 89 661.3 0 89 218 111 661.3 0 111 219 109 661.3 0 109 220 74 661.3 0 74 221 61 661.3 0 61 222 72 661.3 0 72 223 100 661.3 0 100 224 82 661.3 0 82 225 71 661.3 0 71 226 67 661.3 0 67 227 93 661.3 0 93 228 84 661.3 0 84 229 86 661.3 0 86 230 63 661.3 0 63 231 48 661.3 0 48 232 84 661.3 0 84 233 82 661.3 0 82 234 74 661.3 0 74 235 95 661.3 0 95 236 116 661.3 0 116 237 83 661.3 0 83 238 94 661.3 0 94 239 131 661.3 0 131 240 119 661.3 0 119 241 86 661.3 0 86 242 58 661.3 0 58 243 90 661.3 0 90 244 85 661.3 0 85 245 94 661.3 0 94 246 89 661.3 0 89 247 98 661.3 0 98 248 58 661.3 0 58 249 75 661.3 0 75 250 43 661.3 0 43 251 65 661.3 0 65 252 75 661.3 0 75 253 110 661.3 0 110 254 82 661.3 0 82 255 91 661.3 0 91 256 83 661.3 0 83 257 120 661.3 0 120 258 72 661.3 0 72 259 73 661.3 0 73 260 56 661.3 0 56 261 80 661.3 0 80 262 93 661.3 0 93 263 162 661.3 0 162 264 77 661.3 0 77 265 89 661.3 0 89 266 95 661.3 0 95 267 122 661.3 0 122 268 102 661.3 0 102 269 64 661.3 0 64 270 60 661.3 0 60 271 306 661.3 0 306 272 865 661.3 0 865 273 187 661.3 0 187 274 100 661.3 0 100 275 80 661.3 0 80 276 71 661.3 0 71 277 50 661.3 0 50 278 82 661.3 0 82 279 121 661.3 0 121 280 111 661.3 0 111 281 64 661.3 0 64 282 45 661.3 0 45 283 102 661.3 0 102 284 90 661.3 0 90 285 65 661.3 0 65 286 156 661.3 0 156 287 154 661.3 0 154 288 149 661.3 0 149 289 148 661.3 0 148 290 99 661.3 0 99 291 133 661.3 0 133 292 137 661.3 0 137 293 113 661.3 0 113 294 84 661.3 0 84 295 61 661.3 0 61 296 110 661.3 0 110 297 102 661.3 0 102 298 97 661.3 0 97 299 113 661.3 0 113 300 89 661.3 0 89 301 26 661.3 0 26 Finished in 5.28 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 117,389 Reads with adapters: 72,272 (61.6%) Reads that were too short: 64 (0.1%) Reads written (passing filters): 72,208 (61.5%) Total basepairs processed: 30,248,500 bp Total written (filtered): 19,784,199 bp (65.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 72272 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 31 0.0 3 0 0 25 5 1 19 779 0.0 3 0 741 26 10 2 20 71039 0.0 4 69656 1187 140 38 18 21 209 0.0 4 23 182 2 1 1 22 25 0.0 4 4 1 11 1 8 23 62 0.0 4 1 1 1 52 7 24 7 0.0 4 2 0 0 0 5 25 44 0.0 4 0 0 0 0 44 26 1 0.0 4 0 1 29 1 0.0 4 1 36 1 0.0 4 1 105 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 117 4 0.0 4 0 0 0 0 4 140 1 0.0 4 0 0 0 0 1 141 50 0.0 4 0 0 0 0 50 142 2 0.0 4 0 0 0 0 2 143 2 0.0 4 0 0 0 0 2 145 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 162 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.48 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 72,208 Reads with adapters: 2,525 (3.5%) Reads written (passing filters): 72,208 (100.0%) Total basepairs processed: 19,784,199 bp Total written (filtered): 19,719,572 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2525 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.3% C: 4.7% G: 85.9% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 1128.2 0 27 4 3 282.1 0 3 5 2 70.5 0 2 6 1 17.6 0 1 9 1 0.3 0 0 1 10 3 0.1 1 0 3 11 2187 0.0 1 9 2178 12 135 0.0 1 0 135 13 4 0.0 1 0 4 15 1 0.0 1 0 1 152 23 0.0 1 18 5 153 2 0.0 1 2 160 1 0.0 1 0 1 210 9 0.0 1 7 2 258 1 0.0 1 1 260 1 0.0 1 1 261 123 0.0 1 117 6 262 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.