This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.42 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 177,871 (6.6%) Reads that were too short: 32,340 (1.2%) Reads written (passing filters): 145,531 (5.4%) Total basepairs processed: 815,312,680 bp Total written (filtered): 32,361,664 bp (4.0%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 177871 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1806 661.3 0 1806 7 6680 661.3 0 6680 8 1152 661.3 0 1152 9 60787 661.3 0 60787 10 97 661.3 0 97 11 32 661.3 0 32 12 40 661.3 0 40 13 27 661.3 0 27 14 29 661.3 0 29 15 25 661.3 0 25 16 12 661.3 0 12 17 30 661.3 0 30 18 30 661.3 0 30 19 30 661.3 0 30 20 22 661.3 0 22 21 26 661.3 0 26 22 18 661.3 0 18 23 34 661.3 0 34 24 20 661.3 0 20 25 24 661.3 0 24 26 36 661.3 0 36 27 25 661.3 0 25 28 27 661.3 0 27 29 21 661.3 0 21 30 22 661.3 0 22 31 27 661.3 0 27 32 26 661.3 0 26 33 22 661.3 0 22 34 56 661.3 0 56 35 18 661.3 0 18 36 19 661.3 0 19 37 22 661.3 0 22 38 39 661.3 0 39 39 35 661.3 0 35 40 35 661.3 0 35 41 191 661.3 0 191 42 30 661.3 0 30 43 42 661.3 0 42 44 41 661.3 0 41 45 131 661.3 0 131 46 191 661.3 0 191 47 30 661.3 0 30 48 33 661.3 0 33 49 21 661.3 0 21 50 37 661.3 0 37 51 23 661.3 0 23 52 27 661.3 0 27 53 24 661.3 0 24 54 25 661.3 0 25 55 22 661.3 0 22 56 38 661.3 0 38 57 26 661.3 0 26 58 44 661.3 0 44 59 34 661.3 0 34 60 31 661.3 0 31 61 22 661.3 0 22 62 51 661.3 0 51 63 39 661.3 0 39 64 38 661.3 0 38 65 24 661.3 0 24 66 34 661.3 0 34 67 33 661.3 0 33 68 25 661.3 0 25 69 30 661.3 0 30 70 32 661.3 0 32 71 47 661.3 0 47 72 33 661.3 0 33 73 99 661.3 0 99 74 27 661.3 0 27 75 35 661.3 0 35 76 43 661.3 0 43 77 31 661.3 0 31 78 30 661.3 0 30 79 31 661.3 0 31 80 29 661.3 0 29 81 45 661.3 0 45 82 52 661.3 0 52 83 91 661.3 0 91 84 61 661.3 0 61 85 65 661.3 0 65 86 80 661.3 0 80 87 223 661.3 0 223 88 167 661.3 0 167 89 295 661.3 0 295 90 231 661.3 0 231 91 217 661.3 0 217 92 74 661.3 0 74 93 66 661.3 0 66 94 106 661.3 0 106 95 60 661.3 0 60 96 47 661.3 0 47 97 64 661.3 0 64 98 52 661.3 0 52 99 94 661.3 0 94 100 211 661.3 0 211 101 171 661.3 0 171 102 101 661.3 0 101 103 194 661.3 0 194 104 151 661.3 0 151 105 161 661.3 0 161 106 111 661.3 0 111 107 156 661.3 0 156 108 162 661.3 0 162 109 65 661.3 0 65 110 72 661.3 0 72 111 71 661.3 0 71 112 107 661.3 0 107 113 80 661.3 0 80 114 89 661.3 0 89 115 102 661.3 0 102 116 98 661.3 0 98 117 74 661.3 0 74 118 80 661.3 0 80 119 97 661.3 0 97 120 121 661.3 0 121 121 119 661.3 0 119 122 102 661.3 0 102 123 133 661.3 0 133 124 133 661.3 0 133 125 110 661.3 0 110 126 106 661.3 0 106 127 109 661.3 0 109 128 127 661.3 0 127 129 182 661.3 0 182 130 141 661.3 0 141 131 162 661.3 0 162 132 327 661.3 0 327 133 250 661.3 0 250 134 178 661.3 0 178 135 288 661.3 0 288 136 148 661.3 0 148 137 133 661.3 0 133 138 208 661.3 0 208 139 203 661.3 0 203 140 1149 661.3 0 1149 141 722 661.3 0 722 142 466 661.3 0 466 143 277 661.3 0 277 144 454 661.3 0 454 145 872 661.3 0 872 146 9357 661.3 0 9357 147 3355 661.3 0 3355 148 12461 661.3 0 12461 149 877 661.3 0 877 150 10015 661.3 0 10015 151 4514 661.3 0 4514 152 10381 661.3 0 10381 153 184 661.3 0 184 154 149 661.3 0 149 155 215 661.3 0 215 156 1221 661.3 0 1221 157 513 661.3 0 513 158 846 661.3 0 846 159 180 661.3 0 180 160 227 661.3 0 227 161 308 661.3 0 308 162 192 661.3 0 192 163 106 661.3 0 106 164 159 661.3 0 159 165 174 661.3 0 174 166 196 661.3 0 196 167 94 661.3 0 94 168 169 661.3 0 169 169 232 661.3 0 232 170 138 661.3 0 138 171 95 661.3 0 95 172 68 661.3 0 68 173 71 661.3 0 71 174 116 661.3 0 116 175 126 661.3 0 126 176 92 661.3 0 92 177 126 661.3 0 126 178 108 661.3 0 108 179 124 661.3 0 124 180 147 661.3 0 147 181 262 661.3 0 262 182 301 661.3 0 301 183 188 661.3 0 188 184 237 661.3 0 237 185 188 661.3 0 188 186 271 661.3 0 271 187 227 661.3 0 227 188 209 661.3 0 209 189 152 661.3 0 152 190 177 661.3 0 177 191 165 661.3 0 165 192 85 661.3 0 85 193 71 661.3 0 71 194 84 661.3 0 84 195 104 661.3 0 104 196 100 661.3 0 100 197 124 661.3 0 124 198 122 661.3 0 122 199 85 661.3 0 85 200 99 661.3 0 99 201 156 661.3 0 156 202 227 661.3 0 227 203 174 661.3 0 174 204 177 661.3 0 177 205 203 661.3 0 203 206 207 661.3 0 207 207 270 661.3 0 270 208 516 661.3 0 516 209 397 661.3 0 397 210 232 661.3 0 232 211 230 661.3 0 230 212 594 661.3 0 594 213 344 661.3 0 344 214 290 661.3 0 290 215 205 661.3 0 205 216 210 661.3 0 210 217 126 661.3 0 126 218 152 661.3 0 152 219 171 661.3 0 171 220 546 661.3 0 546 221 552 661.3 0 552 222 506 661.3 0 506 223 275 661.3 0 275 224 223 661.3 0 223 225 329 661.3 0 329 226 307 661.3 0 307 227 178 661.3 0 178 228 188 661.3 0 188 229 131 661.3 0 131 230 205 661.3 0 205 231 218 661.3 0 218 232 225 661.3 0 225 233 239 661.3 0 239 234 279 661.3 0 279 235 256 661.3 0 256 236 540 661.3 0 540 237 436 661.3 0 436 238 311 661.3 0 311 239 157 661.3 0 157 240 133 661.3 0 133 241 189 661.3 0 189 242 162 661.3 0 162 243 315 661.3 0 315 244 286 661.3 0 286 245 148 661.3 0 148 246 123 661.3 0 123 247 120 661.3 0 120 248 85 661.3 0 85 249 61 661.3 0 61 250 126 661.3 0 126 251 223 661.3 0 223 252 216 661.3 0 216 253 186 661.3 0 186 254 338 661.3 0 338 255 616 661.3 0 616 256 3692 661.3 0 3692 257 2839 661.3 0 2839 258 2378 661.3 0 2378 259 627 661.3 0 627 260 236 661.3 0 236 261 297 661.3 0 297 262 308 661.3 0 308 263 232 661.3 0 232 264 252 661.3 0 252 265 188 661.3 0 188 266 274 661.3 0 274 267 190 661.3 0 190 268 125 661.3 0 125 269 104 661.3 0 104 270 175 661.3 0 175 271 172 661.3 0 172 272 165 661.3 0 165 273 150 661.3 0 150 274 172 661.3 0 172 275 157 661.3 0 157 276 150 661.3 0 150 277 163 661.3 0 163 278 177 661.3 0 177 279 228 661.3 0 228 280 451 661.3 0 451 281 487 661.3 0 487 282 405 661.3 0 405 283 518 661.3 0 518 284 384 661.3 0 384 285 314 661.3 0 314 286 131 661.3 0 131 287 75 661.3 0 75 288 97 661.3 0 97 289 87 661.3 0 87 290 230 661.3 0 230 291 161 661.3 0 161 292 93 661.3 0 93 293 171 661.3 0 171 294 144 661.3 0 144 295 76 661.3 0 76 296 125 661.3 0 125 297 176 661.3 0 176 298 207 661.3 0 207 299 158 661.3 0 158 300 154 661.3 0 154 301 92 661.3 0 92 Finished in 5.67 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 145,531 Reads with adapters: 62,274 (42.8%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 62,266 (42.8%) Total basepairs processed: 32,361,664 bp Total written (filtered): 17,125,078 bp (52.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 62274 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 25 0.0 3 0 0 20 5 16 593 0.0 3 0 581 12 17 61394 0.0 3 60239 1124 27 4 18 106 0.0 3 7 85 9 5 19 48 0.0 3 0 0 32 16 20 89 0.0 3 0 0 0 89 29 1 0.0 3 0 0 0 1 30 1 0.0 3 1 32 1 0.0 3 1 34 2 0.0 3 1 0 0 1 46 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 267 1 0.0 3 0 1 275 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.23 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 62,266 Reads with adapters: 5,130 (8.2%) Reads written (passing filters): 62,266 (100.0%) Total basepairs processed: 17,125,078 bp Total written (filtered): 17,109,672 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5130 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.0% C: 3.2% G: 54.0% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5115 972.9 0 5115 4 14 243.2 0 14 5 1 60.8 0 1