This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.88 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 104,836 (3.9%) Reads that were too short: 50,202 (1.9%) Reads written (passing filters): 54,634 (2.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 15,263,042 bp (1.9%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 104836 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1413 661.3 0 1413 7 44569 661.3 0 44569 8 71 661.3 0 71 9 33 661.3 0 33 10 22 661.3 0 22 11 56 661.3 0 56 12 296 661.3 0 296 13 24 661.3 0 24 14 39 661.3 0 39 15 29 661.3 0 29 16 42 661.3 0 42 17 47 661.3 0 47 18 24 661.3 0 24 19 164 661.3 0 164 20 35 661.3 0 35 21 182 661.3 0 182 22 40 661.3 0 40 23 31 661.3 0 31 24 29 661.3 0 29 25 25 661.3 0 25 26 32 661.3 0 32 27 24 661.3 0 24 28 22 661.3 0 22 29 32 661.3 0 32 30 30 661.3 0 30 31 33 661.3 0 33 32 67 661.3 0 67 33 49 661.3 0 49 34 62 661.3 0 62 35 32 661.3 0 32 36 27 661.3 0 27 37 45 661.3 0 45 38 50 661.3 0 50 39 30 661.3 0 30 40 47 661.3 0 47 41 43 661.3 0 43 42 43 661.3 0 43 43 35 661.3 0 35 44 28 661.3 0 28 45 43 661.3 0 43 46 44 661.3 0 44 47 70 661.3 0 70 48 43 661.3 0 43 49 38 661.3 0 38 50 37 661.3 0 37 51 35 661.3 0 35 52 41 661.3 0 41 53 55 661.3 0 55 54 48 661.3 0 48 55 40 661.3 0 40 56 23 661.3 0 23 57 24 661.3 0 24 58 54 661.3 0 54 59 32 661.3 0 32 60 28 661.3 0 28 61 33 661.3 0 33 62 40 661.3 0 40 63 31 661.3 0 31 64 35 661.3 0 35 65 25 661.3 0 25 66 50 661.3 0 50 67 31 661.3 0 31 68 42 661.3 0 42 69 36 661.3 0 36 70 45 661.3 0 45 71 96 661.3 0 96 72 52 661.3 0 52 73 102 661.3 0 102 74 40 661.3 0 40 75 37 661.3 0 37 76 34 661.3 0 34 77 57 661.3 0 57 78 42 661.3 0 42 79 35 661.3 0 35 80 33 661.3 0 33 81 42 661.3 0 42 82 43 661.3 0 43 83 30 661.3 0 30 84 31 661.3 0 31 85 37 661.3 0 37 86 37 661.3 0 37 87 13 661.3 0 13 88 23 661.3 0 23 89 32 661.3 0 32 90 33 661.3 0 33 91 38 661.3 0 38 92 40 661.3 0 40 93 46 661.3 0 46 94 37 661.3 0 37 95 43 661.3 0 43 96 37 661.3 0 37 97 30 661.3 0 30 98 38 661.3 0 38 99 26 661.3 0 26 100 35 661.3 0 35 101 24 661.3 0 24 102 26 661.3 0 26 103 28 661.3 0 28 104 21 661.3 0 21 105 39 661.3 0 39 106 33 661.3 0 33 107 37 661.3 0 37 108 29 661.3 0 29 109 30 661.3 0 30 110 25 661.3 0 25 111 38 661.3 0 38 112 31 661.3 0 31 113 32 661.3 0 32 114 26 661.3 0 26 115 35 661.3 0 35 116 39 661.3 0 39 117 57 661.3 0 57 118 28 661.3 0 28 119 24 661.3 0 24 120 38 661.3 0 38 121 30 661.3 0 30 122 25 661.3 0 25 123 39 661.3 0 39 124 136 661.3 0 136 125 29 661.3 0 29 126 42 661.3 0 42 127 58 661.3 0 58 128 31 661.3 0 31 129 30 661.3 0 30 130 70 661.3 0 70 131 207 661.3 0 207 132 580 661.3 0 580 133 60 661.3 0 60 134 33 661.3 0 33 135 41 661.3 0 41 136 41 661.3 0 41 137 126 661.3 0 126 138 188 661.3 0 188 139 45 661.3 0 45 140 37 661.3 0 37 141 18 661.3 0 18 142 38 661.3 0 38 143 35 661.3 0 35 144 23 661.3 0 23 145 34 661.3 0 34 146 30 661.3 0 30 147 27 661.3 0 27 148 36 661.3 0 36 149 26 661.3 0 26 150 26 661.3 0 26 151 51 661.3 0 51 152 65 661.3 0 65 153 80 661.3 0 80 154 30 661.3 0 30 155 39 661.3 0 39 156 27 661.3 0 27 157 32 661.3 0 32 158 37 661.3 0 37 159 25 661.3 0 25 160 12 661.3 0 12 161 27 661.3 0 27 162 43 661.3 0 43 163 21 661.3 0 21 164 36 661.3 0 36 165 30 661.3 0 30 166 26 661.3 0 26 167 41 661.3 0 41 168 40 661.3 0 40 169 22 661.3 0 22 170 31 661.3 0 31 171 30 661.3 0 30 172 28 661.3 0 28 173 30 661.3 0 30 174 26 661.3 0 26 175 23 661.3 0 23 176 41 661.3 0 41 177 28 661.3 0 28 178 49 661.3 0 49 179 31 661.3 0 31 180 18 661.3 0 18 181 25 661.3 0 25 182 21 661.3 0 21 183 30 661.3 0 30 184 44 661.3 0 44 185 27 661.3 0 27 186 35 661.3 0 35 187 32 661.3 0 32 188 38 661.3 0 38 189 41 661.3 0 41 190 39 661.3 0 39 191 29 661.3 0 29 192 29 661.3 0 29 193 20 661.3 0 20 194 37 661.3 0 37 195 27 661.3 0 27 196 39 661.3 0 39 197 36 661.3 0 36 198 41 661.3 0 41 199 34 661.3 0 34 200 32 661.3 0 32 201 34 661.3 0 34 202 46 661.3 0 46 203 172 661.3 0 172 204 144 661.3 0 144 205 88 661.3 0 88 206 44 661.3 0 44 207 39 661.3 0 39 208 23 661.3 0 23 209 26 661.3 0 26 210 28 661.3 0 28 211 42 661.3 0 42 212 156 661.3 0 156 213 282 661.3 0 282 214 356 661.3 0 356 215 47 661.3 0 47 216 38 661.3 0 38 217 45 661.3 0 45 218 62 661.3 0 62 219 25 661.3 0 25 220 57 661.3 0 57 221 31 661.3 0 31 222 36 661.3 0 36 223 46 661.3 0 46 224 40 661.3 0 40 225 96 661.3 0 96 226 46 661.3 0 46 227 36 661.3 0 36 228 77 661.3 0 77 229 36 661.3 0 36 230 30 661.3 0 30 231 39 661.3 0 39 232 67 661.3 0 67 233 58 661.3 0 58 234 53 661.3 0 53 235 138 661.3 0 138 236 341 661.3 0 341 237 341 661.3 0 341 238 330 661.3 0 330 239 75 661.3 0 75 240 30 661.3 0 30 241 47 661.3 0 47 242 32 661.3 0 32 243 100 661.3 0 100 244 130 661.3 0 130 245 188 661.3 0 188 246 103 661.3 0 103 247 65 661.3 0 65 248 35 661.3 0 35 249 36 661.3 0 36 250 50 661.3 0 50 251 41 661.3 0 41 252 50 661.3 0 50 253 43 661.3 0 43 254 73 661.3 0 73 255 79 661.3 0 79 256 95 661.3 0 95 257 58 661.3 0 58 258 53 661.3 0 53 259 82 661.3 0 82 260 223 661.3 0 223 261 221 661.3 0 221 262 1995 661.3 0 1995 263 177 661.3 0 177 264 194 661.3 0 194 265 170 661.3 0 170 266 57 661.3 0 57 267 71 661.3 0 71 268 90 661.3 0 90 269 107 661.3 0 107 270 98 661.3 0 98 271 108 661.3 0 108 272 199 661.3 0 199 273 448 661.3 0 448 274 127 661.3 0 127 275 190 661.3 0 190 276 342 661.3 0 342 277 184 661.3 0 184 278 168 661.3 0 168 279 323 661.3 0 323 280 349 661.3 0 349 281 336 661.3 0 336 282 303 661.3 0 303 283 338 661.3 0 338 284 329 661.3 0 329 285 476 661.3 0 476 286 5455 661.3 0 5455 287 11971 661.3 0 11971 288 19464 661.3 0 19464 289 147 661.3 0 147 290 41 661.3 0 41 291 38 661.3 0 38 292 40 661.3 0 40 293 31 661.3 0 31 294 40 661.3 0 40 295 69 661.3 0 69 296 53 661.3 0 53 297 66 661.3 0 66 298 56 661.3 0 56 299 30 661.3 0 30 300 26 661.3 0 26 301 26 661.3 0 26 Finished in 2.55 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 54,634 Reads with adapters: 45,260 (82.8%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 45,259 (82.8%) Total basepairs processed: 15,263,042 bp Total written (filtered): 12,402,452 bp (81.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45260 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 15 0.0 3 0 0 13 2 19 506 0.0 3 0 489 9 7 1 20 44576 0.0 4 43708 762 70 15 21 21 103 0.0 4 12 84 3 2 2 22 12 0.0 4 0 0 10 1 1 23 36 0.0 4 2 0 0 30 4 24 9 0.0 4 3 0 0 0 6 45 2 0.0 4 2 163 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 45,259 Reads with adapters: 3,761 (8.3%) Reads written (passing filters): 45,259 (100.0%) Total basepairs processed: 12,402,452 bp Total written (filtered): 11,742,906 bp (94.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3761 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 22.2% C: 1.2% G: 33.5% T: 0.6% none/other: 42.5% Overview of removed sequences length count expect max.err error counts 3 29 707.2 0 29 4 2 176.8 0 2 9 2 0.2 0 0 2 10 3 0.0 1 0 3 11 1258 0.0 1 5 1253 12 73 0.0 1 2 71 13 1 0.0 1 0 1 14 1 0.0 1 0 1 20 1 0.0 1 0 1 171 1 0.0 1 1 210 5 0.0 1 4 1 211 1 0.0 1 1 225 7 0.0 1 6 1 249 1 0.0 1 1 259 1 0.0 1 1 260 3 0.0 1 3 261 410 0.0 1 380 30 262 350 0.0 1 328 22 263 6 0.0 1 6 265 1 0.0 1 1 271 7 0.0 1 7 273 3 0.0 1 3 274 1547 0.0 1 1470 77 275 48 0.0 1 46 2