This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.53 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 586,910 (21.7%) Reads that were too short: 215,698 (8.0%) Reads written (passing filters): 371,212 (13.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 68,813,724 bp (8.4%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 586910 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 989 661.3 0 989 7 17195 661.3 0 17195 8 1027 661.3 0 1027 9 44638 661.3 0 44638 10 77 661.3 0 77 11 18 661.3 0 18 12 16 661.3 0 16 13 14 661.3 0 14 14 18 661.3 0 18 15 15 661.3 0 15 16 29 661.3 0 29 17 25 661.3 0 25 18 11 661.3 0 11 19 30 661.3 0 30 20 17 661.3 0 17 21 26 661.3 0 26 22 24 661.3 0 24 23 20 661.3 0 20 24 15 661.3 0 15 25 26 661.3 0 26 26 22 661.3 0 22 27 26 661.3 0 26 28 39 661.3 0 39 29 28 661.3 0 28 30 27 661.3 0 27 31 33 661.3 0 33 32 78 661.3 0 78 33 35 661.3 0 35 34 30 661.3 0 30 35 20 661.3 0 20 36 29 661.3 0 29 37 37 661.3 0 37 38 19 661.3 0 19 39 22 661.3 0 22 40 116 661.3 0 116 41 52 661.3 0 52 42 90 661.3 0 90 43 95 661.3 0 95 44 32 661.3 0 32 45 47 661.3 0 47 46 36 661.3 0 36 47 28 661.3 0 28 48 46 661.3 0 46 49 54 661.3 0 54 50 136 661.3 0 136 51 1518 661.3 0 1518 52 1401 661.3 0 1401 53 15 661.3 0 15 54 11 661.3 0 11 55 16 661.3 0 16 56 28 661.3 0 28 57 24 661.3 0 24 58 23 661.3 0 23 59 14 661.3 0 14 60 14 661.3 0 14 61 17 661.3 0 17 62 16 661.3 0 16 63 29 661.3 0 29 64 21 661.3 0 21 65 15 661.3 0 15 66 32 661.3 0 32 67 30 661.3 0 30 68 96 661.3 0 96 69 108 661.3 0 108 70 29 661.3 0 29 71 59 661.3 0 59 72 33 661.3 0 33 73 29 661.3 0 29 74 29 661.3 0 29 75 39 661.3 0 39 76 35 661.3 0 35 77 46 661.3 0 46 78 64 661.3 0 64 79 277 661.3 0 277 80 925 661.3 0 925 81 1264 661.3 0 1264 82 1935 661.3 0 1935 83 3053 661.3 0 3053 84 2955 661.3 0 2955 85 4673 661.3 0 4673 86 3346 661.3 0 3346 87 2150 661.3 0 2150 88 1696 661.3 0 1696 89 1464 661.3 0 1464 90 1350 661.3 0 1350 91 3976 661.3 0 3976 92 2073 661.3 0 2073 93 3047 661.3 0 3047 94 1798 661.3 0 1798 95 3474 661.3 0 3474 96 2448 661.3 0 2448 97 983 661.3 0 983 98 804 661.3 0 804 99 935 661.3 0 935 100 843 661.3 0 843 101 1572 661.3 0 1572 102 1899 661.3 0 1899 103 3113 661.3 0 3113 104 3962 661.3 0 3962 105 4938 661.3 0 4938 106 3427 661.3 0 3427 107 3275 661.3 0 3275 108 1762 661.3 0 1762 109 2740 661.3 0 2740 110 1855 661.3 0 1855 111 2691 661.3 0 2691 112 2762 661.3 0 2762 113 3104 661.3 0 3104 114 2747 661.3 0 2747 115 2545 661.3 0 2545 116 2180 661.3 0 2180 117 1949 661.3 0 1949 118 2899 661.3 0 2899 119 3215 661.3 0 3215 120 2866 661.3 0 2866 121 2495 661.3 0 2495 122 2209 661.3 0 2209 123 2373 661.3 0 2373 124 2782 661.3 0 2782 125 3578 661.3 0 3578 126 3491 661.3 0 3491 127 2684 661.3 0 2684 128 2463 661.3 0 2463 129 1912 661.3 0 1912 130 2242 661.3 0 2242 131 1943 661.3 0 1943 132 2534 661.3 0 2534 133 4940 661.3 0 4940 134 4359 661.3 0 4359 135 3449 661.3 0 3449 136 2213 661.3 0 2213 137 1944 661.3 0 1944 138 1880 661.3 0 1880 139 1965 661.3 0 1965 140 2192 661.3 0 2192 141 2139 661.3 0 2139 142 2002 661.3 0 2002 143 1990 661.3 0 1990 144 2141 661.3 0 2141 145 2559 661.3 0 2559 146 2815 661.3 0 2815 147 2637 661.3 0 2637 148 3317 661.3 0 3317 149 3185 661.3 0 3185 150 2668 661.3 0 2668 151 2779 661.3 0 2779 152 2771 661.3 0 2771 153 3224 661.3 0 3224 154 2972 661.3 0 2972 155 2573 661.3 0 2573 156 2439 661.3 0 2439 157 2222 661.3 0 2222 158 2251 661.3 0 2251 159 2550 661.3 0 2550 160 2698 661.3 0 2698 161 2948 661.3 0 2948 162 4349 661.3 0 4349 163 4227 661.3 0 4227 164 3625 661.3 0 3625 165 2837 661.3 0 2837 166 2026 661.3 0 2026 167 2080 661.3 0 2080 168 1752 661.3 0 1752 169 2170 661.3 0 2170 170 2417 661.3 0 2417 171 2478 661.3 0 2478 172 2734 661.3 0 2734 173 2801 661.3 0 2801 174 2303 661.3 0 2303 175 1790 661.3 0 1790 176 1737 661.3 0 1737 177 1601 661.3 0 1601 178 2274 661.3 0 2274 179 2368 661.3 0 2368 180 2370 661.3 0 2370 181 2410 661.3 0 2410 182 2106 661.3 0 2106 183 2354 661.3 0 2354 184 1973 661.3 0 1973 185 2131 661.3 0 2131 186 2223 661.3 0 2223 187 1952 661.3 0 1952 188 1928 661.3 0 1928 189 2172 661.3 0 2172 190 2591 661.3 0 2591 191 2778 661.3 0 2778 192 2631 661.3 0 2631 193 2580 661.3 0 2580 194 1657 661.3 0 1657 195 1270 661.3 0 1270 196 1334 661.3 0 1334 197 1499 661.3 0 1499 198 2648 661.3 0 2648 199 2713 661.3 0 2713 200 1780 661.3 0 1780 201 1847 661.3 0 1847 202 1587 661.3 0 1587 203 1984 661.3 0 1984 204 1676 661.3 0 1676 205 1576 661.3 0 1576 206 1793 661.3 0 1793 207 1816 661.3 0 1816 208 1684 661.3 0 1684 209 1653 661.3 0 1653 210 1512 661.3 0 1512 211 1830 661.3 0 1830 212 2677 661.3 0 2677 213 14136 661.3 0 14136 214 4156 661.3 0 4156 215 9784 661.3 0 9784 216 2277 661.3 0 2277 217 2077 661.3 0 2077 218 2123 661.3 0 2123 219 2182 661.3 0 2182 220 1920 661.3 0 1920 221 1681 661.3 0 1681 222 2051 661.3 0 2051 223 1830 661.3 0 1830 224 2111 661.3 0 2111 225 2491 661.3 0 2491 226 2290 661.3 0 2290 227 2724 661.3 0 2724 228 2126 661.3 0 2126 229 2439 661.3 0 2439 230 1954 661.3 0 1954 231 1807 661.3 0 1807 232 1493 661.3 0 1493 233 1455 661.3 0 1455 234 1606 661.3 0 1606 235 1897 661.3 0 1897 236 1782 661.3 0 1782 237 1685 661.3 0 1685 238 1876 661.3 0 1876 239 1809 661.3 0 1809 240 1732 661.3 0 1732 241 1525 661.3 0 1525 242 1697 661.3 0 1697 243 1595 661.3 0 1595 244 1335 661.3 0 1335 245 1608 661.3 0 1608 246 2024 661.3 0 2024 247 3285 661.3 0 3285 248 3223 661.3 0 3223 249 2656 661.3 0 2656 250 2549 661.3 0 2549 251 3336 661.3 0 3336 252 2069 661.3 0 2069 253 2172 661.3 0 2172 254 1710 661.3 0 1710 255 1859 661.3 0 1859 256 1711 661.3 0 1711 257 2000 661.3 0 2000 258 1954 661.3 0 1954 259 1792 661.3 0 1792 260 1456 661.3 0 1456 261 1322 661.3 0 1322 262 1314 661.3 0 1314 263 1583 661.3 0 1583 264 1960 661.3 0 1960 265 1959 661.3 0 1959 266 2384 661.3 0 2384 267 3083 661.3 0 3083 268 2451 661.3 0 2451 269 2332 661.3 0 2332 270 1524 661.3 0 1524 271 1929 661.3 0 1929 272 1493 661.3 0 1493 273 1897 661.3 0 1897 274 1871 661.3 0 1871 275 1819 661.3 0 1819 276 1633 661.3 0 1633 277 1518 661.3 0 1518 278 1664 661.3 0 1664 279 2361 661.3 0 2361 280 2102 661.3 0 2102 281 1242 661.3 0 1242 282 1727 661.3 0 1727 283 1393 661.3 0 1393 284 1800 661.3 0 1800 285 1634 661.3 0 1634 286 2016 661.3 0 2016 287 2468 661.3 0 2468 288 1850 661.3 0 1850 289 1742 661.3 0 1742 290 1729 661.3 0 1729 291 2225 661.3 0 2225 292 1808 661.3 0 1808 293 2342 661.3 0 2342 294 1917 661.3 0 1917 295 2139 661.3 0 2139 296 1597 661.3 0 1597 297 1273 661.3 0 1273 298 1030 661.3 0 1030 299 1108 661.3 0 1108 300 1370 661.3 0 1370 301 4251 661.3 0 4251 Finished in 12.16 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 371,212 Reads with adapters: 46,278 (12.5%) Reads that were too short: 74 (0.0%) Reads written (passing filters): 46,204 (12.4%) Total basepairs processed: 68,813,724 bp Total written (filtered): 12,703,123 bp (18.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46278 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 14 0.0 3 0 0 13 1 16 329 0.0 3 0 324 3 2 17 45592 0.0 3 44702 867 21 2 18 141 0.0 3 10 120 9 2 19 47 0.0 3 0 0 30 17 20 45 0.0 3 1 0 0 44 24 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 2 0.0 3 1 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 1 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 1 114 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.62 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 46,204 Reads with adapters: 2,719 (5.9%) Reads written (passing filters): 46,204 (100.0%) Total basepairs processed: 12,703,123 bp Total written (filtered): 12,694,944 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2719 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.0% C: 4.3% G: 48.1% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2702 721.9 0 2702 4 14 180.5 0 14 5 2 45.1 0 2 7 1 2.8 0 1