This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.57 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 66,025 (2.4%) Reads that were too short: 17,222 (0.6%) Reads written (passing filters): 48,803 (1.8%) Total basepairs processed: 815,312,680 bp Total written (filtered): 12,023,348 bp (1.5%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 66025 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 251 661.3 0 251 7 360 661.3 0 360 8 754 661.3 0 754 9 27836 661.3 0 27836 10 215 661.3 0 215 11 48 661.3 0 48 12 41 661.3 0 41 13 53 661.3 0 53 14 56 661.3 0 56 15 47 661.3 0 47 16 56 661.3 0 56 17 39 661.3 0 39 18 43 661.3 0 43 19 56 661.3 0 56 20 52 661.3 0 52 21 58 661.3 0 58 22 55 661.3 0 55 23 52 661.3 0 52 24 43 661.3 0 43 25 32 661.3 0 32 26 43 661.3 0 43 27 52 661.3 0 52 28 50 661.3 0 50 29 44 661.3 0 44 30 50 661.3 0 50 31 37 661.3 0 37 32 48 661.3 0 48 33 53 661.3 0 53 34 55 661.3 0 55 35 41 661.3 0 41 36 57 661.3 0 57 37 50 661.3 0 50 38 74 661.3 0 74 39 49 661.3 0 49 40 68 661.3 0 68 41 53 661.3 0 53 42 70 661.3 0 70 43 45 661.3 0 45 44 31 661.3 0 31 45 31 661.3 0 31 46 46 661.3 0 46 47 56 661.3 0 56 48 45 661.3 0 45 49 52 661.3 0 52 50 41 661.3 0 41 51 61 661.3 0 61 52 57 661.3 0 57 53 50 661.3 0 50 54 50 661.3 0 50 55 53 661.3 0 53 56 56 661.3 0 56 57 65 661.3 0 65 58 64 661.3 0 64 59 51 661.3 0 51 60 71 661.3 0 71 61 57 661.3 0 57 62 70 661.3 0 70 63 51 661.3 0 51 64 42 661.3 0 42 65 48 661.3 0 48 66 94 661.3 0 94 67 78 661.3 0 78 68 70 661.3 0 70 69 33 661.3 0 33 70 48 661.3 0 48 71 52 661.3 0 52 72 57 661.3 0 57 73 79 661.3 0 79 74 119 661.3 0 119 75 83 661.3 0 83 76 80 661.3 0 80 77 47 661.3 0 47 78 88 661.3 0 88 79 82 661.3 0 82 80 84 661.3 0 84 81 52 661.3 0 52 82 63 661.3 0 63 83 50 661.3 0 50 84 71 661.3 0 71 85 80 661.3 0 80 86 51 661.3 0 51 87 94 661.3 0 94 88 92 661.3 0 92 89 77 661.3 0 77 90 57 661.3 0 57 91 118 661.3 0 118 92 67 661.3 0 67 93 81 661.3 0 81 94 229 661.3 0 229 95 237 661.3 0 237 96 125 661.3 0 125 97 325 661.3 0 325 98 398 661.3 0 398 99 367 661.3 0 367 100 306 661.3 0 306 101 68 661.3 0 68 102 61 661.3 0 61 103 62 661.3 0 62 104 69 661.3 0 69 105 50 661.3 0 50 106 59 661.3 0 59 107 55 661.3 0 55 108 67 661.3 0 67 109 91 661.3 0 91 110 78 661.3 0 78 111 256 661.3 0 256 112 648 661.3 0 648 113 472 661.3 0 472 114 90 661.3 0 90 115 76 661.3 0 76 116 59 661.3 0 59 117 70 661.3 0 70 118 72 661.3 0 72 119 63 661.3 0 63 120 71 661.3 0 71 121 62 661.3 0 62 122 76 661.3 0 76 123 50 661.3 0 50 124 74 661.3 0 74 125 67 661.3 0 67 126 94 661.3 0 94 127 94 661.3 0 94 128 234 661.3 0 234 129 173 661.3 0 173 130 115 661.3 0 115 131 144 661.3 0 144 132 207 661.3 0 207 133 322 661.3 0 322 134 243 661.3 0 243 135 120 661.3 0 120 136 107 661.3 0 107 137 72 661.3 0 72 138 60 661.3 0 60 139 73 661.3 0 73 140 93 661.3 0 93 141 90 661.3 0 90 142 78 661.3 0 78 143 53 661.3 0 53 144 75 661.3 0 75 145 63 661.3 0 63 146 138 661.3 0 138 147 98 661.3 0 98 148 93 661.3 0 93 149 104 661.3 0 104 150 68 661.3 0 68 151 61 661.3 0 61 152 73 661.3 0 73 153 57 661.3 0 57 154 101 661.3 0 101 155 168 661.3 0 168 156 197 661.3 0 197 157 101 661.3 0 101 158 59 661.3 0 59 159 66 661.3 0 66 160 82 661.3 0 82 161 72 661.3 0 72 162 83 661.3 0 83 163 64 661.3 0 64 164 90 661.3 0 90 165 88 661.3 0 88 166 176 661.3 0 176 167 113 661.3 0 113 168 124 661.3 0 124 169 74 661.3 0 74 170 66 661.3 0 66 171 67 661.3 0 67 172 77 661.3 0 77 173 51 661.3 0 51 174 115 661.3 0 115 175 99 661.3 0 99 176 76 661.3 0 76 177 80 661.3 0 80 178 86 661.3 0 86 179 223 661.3 0 223 180 186 661.3 0 186 181 227 661.3 0 227 182 86 661.3 0 86 183 73 661.3 0 73 184 83 661.3 0 83 185 104 661.3 0 104 186 122 661.3 0 122 187 89 661.3 0 89 188 63 661.3 0 63 189 65 661.3 0 65 190 87 661.3 0 87 191 69 661.3 0 69 192 97 661.3 0 97 193 291 661.3 0 291 194 693 661.3 0 693 195 370 661.3 0 370 196 447 661.3 0 447 197 85 661.3 0 85 198 121 661.3 0 121 199 157 661.3 0 157 200 192 661.3 0 192 201 162 661.3 0 162 202 133 661.3 0 133 203 78 661.3 0 78 204 98 661.3 0 98 205 125 661.3 0 125 206 116 661.3 0 116 207 122 661.3 0 122 208 135 661.3 0 135 209 96 661.3 0 96 210 86 661.3 0 86 211 97 661.3 0 97 212 106 661.3 0 106 213 97 661.3 0 97 214 94 661.3 0 94 215 116 661.3 0 116 216 125 661.3 0 125 217 179 661.3 0 179 218 101 661.3 0 101 219 117 661.3 0 117 220 297 661.3 0 297 221 329 661.3 0 329 222 352 661.3 0 352 223 157 661.3 0 157 224 99 661.3 0 99 225 83 661.3 0 83 226 104 661.3 0 104 227 136 661.3 0 136 228 115 661.3 0 115 229 105 661.3 0 105 230 105 661.3 0 105 231 98 661.3 0 98 232 79 661.3 0 79 233 97 661.3 0 97 234 94 661.3 0 94 235 107 661.3 0 107 236 117 661.3 0 117 237 292 661.3 0 292 238 503 661.3 0 503 239 752 661.3 0 752 240 99 661.3 0 99 241 110 661.3 0 110 242 135 661.3 0 135 243 195 661.3 0 195 244 231 661.3 0 231 245 132 661.3 0 132 246 153 661.3 0 153 247 106 661.3 0 106 248 121 661.3 0 121 249 121 661.3 0 121 250 132 661.3 0 132 251 89 661.3 0 89 252 193 661.3 0 193 253 119 661.3 0 119 254 387 661.3 0 387 255 369 661.3 0 369 256 788 661.3 0 788 257 232 661.3 0 232 258 162 661.3 0 162 259 112 661.3 0 112 260 94 661.3 0 94 261 97 661.3 0 97 262 417 661.3 0 417 263 793 661.3 0 793 264 1880 661.3 0 1880 265 104 661.3 0 104 266 100 661.3 0 100 267 114 661.3 0 114 268 323 661.3 0 323 269 346 661.3 0 346 270 426 661.3 0 426 271 91 661.3 0 91 272 97 661.3 0 97 273 106 661.3 0 106 274 80 661.3 0 80 275 76 661.3 0 76 276 90 661.3 0 90 277 61 661.3 0 61 278 42 661.3 0 42 279 68 661.3 0 68 280 110 661.3 0 110 281 97 661.3 0 97 282 71 661.3 0 71 283 69 661.3 0 69 284 67 661.3 0 67 285 60 661.3 0 60 286 77 661.3 0 77 287 70 661.3 0 70 288 73 661.3 0 73 289 51 661.3 0 51 290 53 661.3 0 53 291 75 661.3 0 75 292 74 661.3 0 74 293 101 661.3 0 101 294 390 661.3 0 390 295 108 661.3 0 108 296 34 661.3 0 34 297 28 661.3 0 28 298 35 661.3 0 35 299 37 661.3 0 37 300 43 661.3 0 43 301 66 661.3 0 66 Finished in 2.24 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 48,803 Reads with adapters: 29,052 (59.5%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 29,045 (59.5%) Total basepairs processed: 12,023,348 bp Total written (filtered): 7,901,676 bp (65.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29052 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 10 0.0 3 0 0 8 2 19 408 0.0 3 0 382 17 5 4 20 28515 0.0 4 27173 1192 106 30 14 21 83 0.0 4 16 60 3 2 2 22 6 0.0 4 1 1 2 0 2 23 18 0.0 4 1 0 0 14 3 25 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 1 93 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.99 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 29,045 Reads with adapters: 8,148 (28.1%) Reads written (passing filters): 29,045 (100.0%) Total basepairs processed: 7,901,676 bp Total written (filtered): 6,159,292 bp (77.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8148 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 0.9% G: 97.0% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 113.5 0 1 6 1 7.1 0 1 9 242 0.1 0 25 217 10 684 0.0 1 29 655 11 584 0.0 1 3 581 12 1 0.0 1 0 1 150 8 0.0 1 2 6 152 1 0.0 1 1 159 1 0.0 1 0 1 189 1 0.0 1 0 1 207 1 0.0 1 1 208 72 0.0 1 65 7 209 5 0.0 1 4 1 211 1 0.0 1 1 257 1 0.0 1 1 258 3 0.0 1 3 259 1 0.0 1 1 260 36 0.0 1 34 2 261 6132 0.0 1 5895 237 262 239 0.0 1 231 8 263 62 0.0 1 61 1 264 66 0.0 1 60 6 265 3 0.0 1 2 1 266 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.