This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.90 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 449,266 (16.6%) Reads that were too short: 402,565 (14.9%) Reads written (passing filters): 46,701 (1.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 11,708,719 bp (1.4%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 449266 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 253 661.3 0 253 7 832 661.3 0 832 8 30200 661.3 0 30200 9 84 661.3 0 84 10 46 661.3 0 46 11 47 661.3 0 47 12 43 661.3 0 43 13 31 661.3 0 31 14 40 661.3 0 40 15 47 661.3 0 47 16 43 661.3 0 43 17 56 661.3 0 56 18 51 661.3 0 51 19 46 661.3 0 46 20 52 661.3 0 52 21 47 661.3 0 47 22 54 661.3 0 54 23 49 661.3 0 49 24 43 661.3 0 43 25 41 661.3 0 41 26 53 661.3 0 53 27 41 661.3 0 41 28 41 661.3 0 41 29 59 661.3 0 59 30 66 661.3 0 66 31 38 661.3 0 38 32 39 661.3 0 39 33 58 661.3 0 58 34 34 661.3 0 34 35 42 661.3 0 42 36 46 661.3 0 46 37 49 661.3 0 49 38 52 661.3 0 52 39 54 661.3 0 54 40 35 661.3 0 35 41 39 661.3 0 39 42 37 661.3 0 37 43 38 661.3 0 38 44 29 661.3 0 29 45 35 661.3 0 35 46 46 661.3 0 46 47 38 661.3 0 38 48 49 661.3 0 49 49 46 661.3 0 46 50 37 661.3 0 37 51 39 661.3 0 39 52 37 661.3 0 37 53 47 661.3 0 47 54 38 661.3 0 38 55 95 661.3 0 95 56 62 661.3 0 62 57 46 661.3 0 46 58 30 661.3 0 30 59 29 661.3 0 29 60 46 661.3 0 46 61 36 661.3 0 36 62 34 661.3 0 34 63 74 661.3 0 74 64 44 661.3 0 44 65 48 661.3 0 48 66 48 661.3 0 48 67 57 661.3 0 57 68 41 661.3 0 41 69 32 661.3 0 32 70 38 661.3 0 38 71 44 661.3 0 44 72 37 661.3 0 37 73 37 661.3 0 37 74 42 661.3 0 42 75 49 661.3 0 49 76 50 661.3 0 50 77 58 661.3 0 58 78 35 661.3 0 35 79 59 661.3 0 59 80 35 661.3 0 35 81 42 661.3 0 42 82 52 661.3 0 52 83 40 661.3 0 40 84 41 661.3 0 41 85 53 661.3 0 53 86 37 661.3 0 37 87 46 661.3 0 46 88 42 661.3 0 42 89 60 661.3 0 60 90 44 661.3 0 44 91 43 661.3 0 43 92 54 661.3 0 54 93 114 661.3 0 114 94 51 661.3 0 51 95 32 661.3 0 32 96 40 661.3 0 40 97 36 661.3 0 36 98 57 661.3 0 57 99 52 661.3 0 52 100 41 661.3 0 41 101 40 661.3 0 40 102 54 661.3 0 54 103 38 661.3 0 38 104 46 661.3 0 46 105 61 661.3 0 61 106 41 661.3 0 41 107 38 661.3 0 38 108 42 661.3 0 42 109 53 661.3 0 53 110 43 661.3 0 43 111 45 661.3 0 45 112 39 661.3 0 39 113 48 661.3 0 48 114 46 661.3 0 46 115 44 661.3 0 44 116 48 661.3 0 48 117 55 661.3 0 55 118 41 661.3 0 41 119 48 661.3 0 48 120 53 661.3 0 53 121 48 661.3 0 48 122 61 661.3 0 61 123 65 661.3 0 65 124 138 661.3 0 138 125 72 661.3 0 72 126 122 661.3 0 122 127 66 661.3 0 66 128 62 661.3 0 62 129 68 661.3 0 68 130 100 661.3 0 100 131 81 661.3 0 81 132 271 661.3 0 271 133 169 661.3 0 169 134 179 661.3 0 179 135 58 661.3 0 58 136 43 661.3 0 43 137 67 661.3 0 67 138 39 661.3 0 39 139 51 661.3 0 51 140 54 661.3 0 54 141 63 661.3 0 63 142 76 661.3 0 76 143 62 661.3 0 62 144 63 661.3 0 63 145 72 661.3 0 72 146 58 661.3 0 58 147 58 661.3 0 58 148 86 661.3 0 86 149 66 661.3 0 66 150 68 661.3 0 68 151 46 661.3 0 46 152 46 661.3 0 46 153 62 661.3 0 62 154 66 661.3 0 66 155 63 661.3 0 63 156 65 661.3 0 65 157 61 661.3 0 61 158 57 661.3 0 57 159 74 661.3 0 74 160 68 661.3 0 68 161 67 661.3 0 67 162 55 661.3 0 55 163 70 661.3 0 70 164 53 661.3 0 53 165 63 661.3 0 63 166 84 661.3 0 84 167 67 661.3 0 67 168 79 661.3 0 79 169 63 661.3 0 63 170 62 661.3 0 62 171 59 661.3 0 59 172 74 661.3 0 74 173 123 661.3 0 123 174 83 661.3 0 83 175 105 661.3 0 105 176 93 661.3 0 93 177 54 661.3 0 54 178 100 661.3 0 100 179 96 661.3 0 96 180 104 661.3 0 104 181 113 661.3 0 113 182 97 661.3 0 97 183 108 661.3 0 108 184 98 661.3 0 98 185 427 661.3 0 427 186 536 661.3 0 536 187 403 661.3 0 403 188 99 661.3 0 99 189 81 661.3 0 81 190 90 661.3 0 90 191 111 661.3 0 111 192 174 661.3 0 174 193 1174 661.3 0 1174 194 371 661.3 0 371 195 783 661.3 0 783 196 107 661.3 0 107 197 98 661.3 0 98 198 84 661.3 0 84 199 130 661.3 0 130 200 328 661.3 0 328 201 168 661.3 0 168 202 99 661.3 0 99 203 74 661.3 0 74 204 77 661.3 0 77 205 90 661.3 0 90 206 75 661.3 0 75 207 82 661.3 0 82 208 83 661.3 0 83 209 74 661.3 0 74 210 91 661.3 0 91 211 100 661.3 0 100 212 97 661.3 0 97 213 162 661.3 0 162 214 118 661.3 0 118 215 112 661.3 0 112 216 128 661.3 0 128 217 135 661.3 0 135 218 185 661.3 0 185 219 302 661.3 0 302 220 235 661.3 0 235 221 289 661.3 0 289 222 380 661.3 0 380 223 504 661.3 0 504 224 1004 661.3 0 1004 225 386 661.3 0 386 226 642 661.3 0 642 227 890 661.3 0 890 228 549 661.3 0 549 229 554 661.3 0 554 230 683 661.3 0 683 231 876 661.3 0 876 232 3558 661.3 0 3558 233 15443 661.3 0 15443 234 4990 661.3 0 4990 235 961 661.3 0 961 236 1181 661.3 0 1181 237 14191 661.3 0 14191 238 19119 661.3 0 19119 239 43818 661.3 0 43818 240 2902 661.3 0 2902 241 8626 661.3 0 8626 242 688 661.3 0 688 243 977 661.3 0 977 244 1841 661.3 0 1841 245 1117 661.3 0 1117 246 773 661.3 0 773 247 1329 661.3 0 1329 248 1149 661.3 0 1149 249 1001 661.3 0 1001 250 1488 661.3 0 1488 251 2472 661.3 0 2472 252 6402 661.3 0 6402 253 6026 661.3 0 6026 254 10377 661.3 0 10377 255 1591 661.3 0 1591 256 2419 661.3 0 2419 257 23244 661.3 0 23244 258 66549 661.3 0 66549 259 108616 661.3 0 108616 260 37290 661.3 0 37290 261 211 661.3 0 211 262 72 661.3 0 72 263 70 661.3 0 70 264 66 661.3 0 66 265 75 661.3 0 75 266 66 661.3 0 66 267 67 661.3 0 67 268 68 661.3 0 68 269 71 661.3 0 71 270 75 661.3 0 75 271 57 661.3 0 57 272 60 661.3 0 60 273 73 661.3 0 73 274 80 661.3 0 80 275 65 661.3 0 65 276 82 661.3 0 82 277 45 661.3 0 45 278 48 661.3 0 48 279 67 661.3 0 67 280 73 661.3 0 73 281 66 661.3 0 66 282 85 661.3 0 85 283 148 661.3 0 148 284 100 661.3 0 100 285 63 661.3 0 63 286 62 661.3 0 62 287 67 661.3 0 67 288 67 661.3 0 67 289 60 661.3 0 60 290 71 661.3 0 71 291 155 661.3 0 155 292 146 661.3 0 146 293 187 661.3 0 187 294 85 661.3 0 85 295 69 661.3 0 69 296 68 661.3 0 68 297 64 661.3 0 64 298 62 661.3 0 62 299 55 661.3 0 55 300 65 661.3 0 65 301 115 661.3 0 115 Finished in 1.99 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 46,701 Reads with adapters: 31,159 (66.7%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 31,153 (66.7%) Total basepairs processed: 11,708,719 bp Total written (filtered): 8,598,248 bp (73.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31159 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 10 16 218 0.0 3 0 214 3 1 17 30502 0.0 3 30129 368 5 18 90 0.0 3 3 73 11 3 19 272 0.0 3 247 2 14 9 20 51 0.0 3 2 1 0 48 21 1 0.0 3 0 0 0 1 22 1 0.0 3 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 1 37 1 0.0 3 1 48 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 1 275 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 31,153 Reads with adapters: 891 (2.9%) Reads written (passing filters): 31,153 (100.0%) Total basepairs processed: 8,598,248 bp Total written (filtered): 8,595,547 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 891 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.0% C: 18.2% G: 15.7% T: 6.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 866 486.8 0 866 4 22 121.7 0 22 5 3 30.4 0 3