This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.89 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 218,461 (8.1%) Reads that were too short: 9,960 (0.4%) Reads written (passing filters): 208,501 (7.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 49,067,050 bp (6.0%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 218461 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 402 661.3 0 402 7 411 661.3 0 411 8 12002 661.3 0 12002 9 149 661.3 0 149 10 83 661.3 0 83 11 96 661.3 0 96 12 317 661.3 0 317 13 79 661.3 0 79 14 159 661.3 0 159 15 79 661.3 0 79 16 68 661.3 0 68 17 64 661.3 0 64 18 90 661.3 0 90 19 80 661.3 0 80 20 85 661.3 0 85 21 72 661.3 0 72 22 82 661.3 0 82 23 103 661.3 0 103 24 90 661.3 0 90 25 86 661.3 0 86 26 91 661.3 0 91 27 67 661.3 0 67 28 93 661.3 0 93 29 235 661.3 0 235 30 77 661.3 0 77 31 64 661.3 0 64 32 63 661.3 0 63 33 72 661.3 0 72 34 64 661.3 0 64 35 81 661.3 0 81 36 92 661.3 0 92 37 96 661.3 0 96 38 168 661.3 0 168 39 412 661.3 0 412 40 143 661.3 0 143 41 275 661.3 0 275 42 130 661.3 0 130 43 241 661.3 0 241 44 516 661.3 0 516 45 172 661.3 0 172 46 164 661.3 0 164 47 108 661.3 0 108 48 172 661.3 0 172 49 222 661.3 0 222 50 411 661.3 0 411 51 579 661.3 0 579 52 819 661.3 0 819 53 1263 661.3 0 1263 54 2088 661.3 0 2088 55 5247 661.3 0 5247 56 90909 661.3 0 90909 57 10084 661.3 0 10084 58 50211 661.3 0 50211 59 316 661.3 0 316 60 77 661.3 0 77 61 74 661.3 0 74 62 266 661.3 0 266 63 71 661.3 0 71 64 112 661.3 0 112 65 54 661.3 0 54 66 75 661.3 0 75 67 61 661.3 0 61 68 132 661.3 0 132 69 57 661.3 0 57 70 69 661.3 0 69 71 63 661.3 0 63 72 86 661.3 0 86 73 69 661.3 0 69 74 65 661.3 0 65 75 84 661.3 0 84 76 51 661.3 0 51 77 58 661.3 0 58 78 54 661.3 0 54 79 63 661.3 0 63 80 61 661.3 0 61 81 85 661.3 0 85 82 86 661.3 0 86 83 144 661.3 0 144 84 141 661.3 0 141 85 123 661.3 0 123 86 92 661.3 0 92 87 253 661.3 0 253 88 197 661.3 0 197 89 201 661.3 0 201 90 109 661.3 0 109 91 56 661.3 0 56 92 96 661.3 0 96 93 99 661.3 0 99 94 58 661.3 0 58 95 76 661.3 0 76 96 84 661.3 0 84 97 73 661.3 0 73 98 60 661.3 0 60 99 59 661.3 0 59 100 60 661.3 0 60 101 78 661.3 0 78 102 43 661.3 0 43 103 87 661.3 0 87 104 97 661.3 0 97 105 110 661.3 0 110 106 54 661.3 0 54 107 51 661.3 0 51 108 72 661.3 0 72 109 87 661.3 0 87 110 68 661.3 0 68 111 96 661.3 0 96 112 54 661.3 0 54 113 87 661.3 0 87 114 78 661.3 0 78 115 64 661.3 0 64 116 57 661.3 0 57 117 77 661.3 0 77 118 85 661.3 0 85 119 128 661.3 0 128 120 136 661.3 0 136 121 99 661.3 0 99 122 86 661.3 0 86 123 123 661.3 0 123 124 822 661.3 0 822 125 926 661.3 0 926 126 1209 661.3 0 1209 127 272 661.3 0 272 128 62 661.3 0 62 129 71 661.3 0 71 130 73 661.3 0 73 131 67 661.3 0 67 132 245 661.3 0 245 133 75 661.3 0 75 134 76 661.3 0 76 135 105 661.3 0 105 136 71 661.3 0 71 137 73 661.3 0 73 138 103 661.3 0 103 139 79 661.3 0 79 140 86 661.3 0 86 141 80 661.3 0 80 142 98 661.3 0 98 143 94 661.3 0 94 144 93 661.3 0 93 145 128 661.3 0 128 146 196 661.3 0 196 147 1274 661.3 0 1274 148 348 661.3 0 348 149 525 661.3 0 525 150 836 661.3 0 836 151 277 661.3 0 277 152 231 661.3 0 231 153 113 661.3 0 113 154 78 661.3 0 78 155 96 661.3 0 96 156 135 661.3 0 135 157 99 661.3 0 99 158 90 661.3 0 90 159 98 661.3 0 98 160 229 661.3 0 229 161 138 661.3 0 138 162 138 661.3 0 138 163 76 661.3 0 76 164 104 661.3 0 104 165 109 661.3 0 109 166 189 661.3 0 189 167 1336 661.3 0 1336 168 388 661.3 0 388 169 496 661.3 0 496 170 203 661.3 0 203 171 134 661.3 0 134 172 161 661.3 0 161 173 227 661.3 0 227 174 168 661.3 0 168 175 212 661.3 0 212 176 237 661.3 0 237 177 298 661.3 0 298 178 126 661.3 0 126 179 88 661.3 0 88 180 128 661.3 0 128 181 125 661.3 0 125 182 81 661.3 0 81 183 87 661.3 0 87 184 90 661.3 0 90 185 100 661.3 0 100 186 105 661.3 0 105 187 88 661.3 0 88 188 124 661.3 0 124 189 113 661.3 0 113 190 84 661.3 0 84 191 138 661.3 0 138 192 174 661.3 0 174 193 149 661.3 0 149 194 135 661.3 0 135 195 342 661.3 0 342 196 367 661.3 0 367 197 363 661.3 0 363 198 251 661.3 0 251 199 528 661.3 0 528 200 3332 661.3 0 3332 201 1123 661.3 0 1123 202 1942 661.3 0 1942 203 132 661.3 0 132 204 61 661.3 0 61 205 66 661.3 0 66 206 86 661.3 0 86 207 82 661.3 0 82 208 71 661.3 0 71 209 69 661.3 0 69 210 42 661.3 0 42 211 62 661.3 0 62 212 66 661.3 0 66 213 83 661.3 0 83 214 71 661.3 0 71 215 65 661.3 0 65 216 58 661.3 0 58 217 86 661.3 0 86 218 74 661.3 0 74 219 114 661.3 0 114 220 102 661.3 0 102 221 105 661.3 0 105 222 67 661.3 0 67 223 69 661.3 0 69 224 88 661.3 0 88 225 76 661.3 0 76 226 89 661.3 0 89 227 79 661.3 0 79 228 58 661.3 0 58 229 183 661.3 0 183 230 126 661.3 0 126 231 152 661.3 0 152 232 285 661.3 0 285 233 159 661.3 0 159 234 237 661.3 0 237 235 57 661.3 0 57 236 51 661.3 0 51 237 49 661.3 0 49 238 50 661.3 0 50 239 68 661.3 0 68 240 87 661.3 0 87 241 80 661.3 0 80 242 127 661.3 0 127 243 69 661.3 0 69 244 77 661.3 0 77 245 47 661.3 0 47 246 67 661.3 0 67 247 49 661.3 0 49 248 62 661.3 0 62 249 52 661.3 0 52 250 68 661.3 0 68 251 71 661.3 0 71 252 86 661.3 0 86 253 114 661.3 0 114 254 70 661.3 0 70 255 80 661.3 0 80 256 63 661.3 0 63 257 41 661.3 0 41 258 40 661.3 0 40 259 32 661.3 0 32 260 26 661.3 0 26 261 72 661.3 0 72 262 75 661.3 0 75 263 69 661.3 0 69 264 85 661.3 0 85 265 87 661.3 0 87 266 82 661.3 0 82 267 55 661.3 0 55 268 54 661.3 0 54 269 75 661.3 0 75 270 57 661.3 0 57 271 75 661.3 0 75 272 61 661.3 0 61 273 133 661.3 0 133 274 66 661.3 0 66 275 89 661.3 0 89 276 78 661.3 0 78 277 64 661.3 0 64 278 93 661.3 0 93 279 93 661.3 0 93 280 80 661.3 0 80 281 62 661.3 0 62 282 94 661.3 0 94 283 153 661.3 0 153 284 86 661.3 0 86 285 62 661.3 0 62 286 33 661.3 0 33 287 43 661.3 0 43 288 48 661.3 0 48 289 62 661.3 0 62 290 76 661.3 0 76 291 91 661.3 0 91 292 202 661.3 0 202 293 200 661.3 0 200 294 92 661.3 0 92 295 53 661.3 0 53 296 40 661.3 0 40 297 33 661.3 0 33 298 51 661.3 0 51 299 44 661.3 0 44 300 43 661.3 0 43 301 61 661.3 0 61 Finished in 9.16 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 208,501 Reads with adapters: 12,648 (6.1%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 12,635 (6.1%) Total basepairs processed: 49,067,050 bp Total written (filtered): 3,449,214 bp (7.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12648 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 11 0.0 3 0 0 9 1 1 19 151 0.0 3 0 138 6 5 2 20 12189 0.0 4 11898 246 34 6 5 21 40 0.0 4 6 30 1 2 1 22 8 0.0 4 0 5 3 23 229 0.0 4 200 10 0 17 2 24 2 0.0 4 1 0 0 0 1 32 1 0.0 4 0 0 0 0 1 76 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 202 6 0.0 4 0 0 0 0 6 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 12,635 Reads with adapters: 1,390 (11.0%) Reads written (passing filters): 12,635 (100.0%) Total basepairs processed: 3,449,214 bp Total written (filtered): 3,418,985 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1390 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 3.6% G: 88.1% T: 3.5% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 197.4 0 1 4 1 49.4 0 1 9 7 0.0 0 0 7 10 82 0.0 1 2 80 11 1226 0.0 1 5 1221 12 3 0.0 1 0 3 18 1 0.0 1 0 1 19 1 0.0 1 1 20 7 0.0 1 2 5 151 2 0.0 1 2 209 4 0.0 1 3 1 256 2 0.0 1 2 257 1 0.0 1 1 260 22 0.0 1 21 1 261 1 0.0 1 1 267 27 0.0 1 25 2 270 1 0.0 1 0 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.